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Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica) BABT
Llamoca-Zárate,Romulo Marino; Ponte,Luiz Ferreira Aguiar; Landsmann,Joerg; Campos,Francisco de Assis Paiva.
We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Opuntia ficus-indica; Prickly-pear; Cactaceae; Transient gene expression; Particle bombardment.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300005
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Gene delivery into plant cells and magnetic separation using magnetite particles OAK
Yoshizumi, Takeshi; Horikawa, Yoh; Kakuta, Hideo; 吉積, 毅; 堀川, 洋; 角田, 英男.
Palavras-chave: Β-glucruronidase (GUS) gene; Magnetite; Magnetic separation; Particle bombardment; Transient gene expression; 一過的遺伝子発現; 磁気分離; 磁性体; 微粒子撃ち込み; Β-グルクロニダーゼ(GUS)遺伝子.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1343
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Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. Repositório Alice
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F..
bitstream/item/216085/1/fpls-11-01228.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Agrobacterium-mediated transformation; Particle bombardment; High-efficiency plant transformation; Embryonic axis; Glycine Max; Genetic transformation.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125013
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Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants Genet. Mol. Biol.
Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena.
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015
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