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Registros recuperados: 8
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Détection et étude de la stabilité de l'ADN de virus de type herpès infectant les huîtres dans des échantillons d'eau ArchiMer
Vigneron, Vassilia.
Depuis 1991, des mortalités massives associées à des infections à herpès virus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Les études ont porté essentiellement sur la mise en évidence de la présence de ce type de virus chez les coquillages d'intérêt économique. Actuellement, l'intérêt se porte sur la persistance du virus dans le milieu marin, et cela dans le but de déterminer si le milieu constitue ou non un moyen de contamination des animaux. Le travail a constitué en une étude sur la persistance de la détection de l'ADN d'herpès virus (OsHV-1) afin de mieux appréhender les modes de dissémination et de transmission du virus. Dans un premier temps, la stabilité de l'ADN purifié et...
Tipo: Text Palavras-chave: Herpèsvirus; Eau de mer; Détection; PCR; Persistance; Stabilité.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00032/14350/11634.pdf
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Determination of ametryn herbicide by bioassay and gas chromatography-mass spectrometry in analysis of residues in drinking water. Repositório Alice
QUEIROZ, R. H. C.; LANCHOTE, V. L.; BONATO, P. S.; TOZZATO, E.; CARVALHO, D. de; GOMES, M. A.; CERDEIRA, A. L..
A simple, rapid, and quantitative bioassay method was compared to a gas chromotography/mass spectrometry (GC/MS) procedure for the analysis of ametryn in surface and groundwater. This method was based on the activity of ametryn in inhibiting the growth of the primary root and shoot of germinating lettuce, Lactuca sativa L. seed. The procedure was sensitive to 0.01 ug/l and was applicable from this concentration up to 0.6 ug/l. Initial surface sterilization of the seed selection of pregerminated seed of certain root lengths, and special equipment are not necessary. So, we concluded that the sensitivity of the bioassay method is compatible with the chromatographic method (GC-MS). However, the study of the correlation between methods suggests that the...
Tipo: Artigo de periódico Palavras-chave: Persistance; Beneficial effects; Contamination; Water samples; Method; Persistent; Movement; Watershed; Environment; Herbicides; Leaching; Monitoring; Toxicity.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/13134
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Devenir des virus entériques humains en milieu marin : Apport des VLPs (Virus Like Particles) pour la purification des coquillages ArchiMer
Loisy, Fabienne.
This work was developed following two research axes: the improvement of norovirus (human and animal strain) and rotavirus detection, and the evaluation of VLPs as a viral substitute for studying their comportment in marine environment. The main point concerning the method optimisation was the development of real-time RT-PCR for norovirus detection. The second part of the work has demonstrated the potential of norovirus and rotavirus VLPs as a viral surrogate as proven by the study of their stability in seawater. As VLPs are non-pathogen particles, the long persistence of rotavirus VLPs in shellfish has been shown in natural conditions (oysters on marine estuaries). The studies of Norwalk virus VLPs attachment to oyster tissues have demonstrated a specific...
Tipo: Text Palavras-chave: Specific binding; Persistence; Shellfish; Real time RT PCR; VLPs; Rotavirus; Norovirus; Fixation spécifique; Persistance; Coquillage; RT PCR en temps réel; VLPs; Rotavirus.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-327.pdf
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Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte ArchiMer
Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf
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Les Escherichia Coli potentiellement pathogenes dans l’environnement littoral : cas des STEC et des EPEC ArchiMer
Baliere, Charlotte.
The contamination of coastal areas by potentially pathogenic enteric bacteria is of concern for the sustainability of some uses, such as shellfish farming, recreational shellfish harvesting and bathing. The contamination of these environments may occur through the land-spreading of livestock wastes, animal feces deposited on pastures, wastewaters from slaughterhouses or from municipal wastewater treatment plants. The presence of these bacteria in coastal environment may present a potential risk to human health. In fact, shellfish-borne outbreaks or human infections may occur by the consumption of shellfish from contaminated areas or the ingestion of water during bathing in contaminated waters, respectively. To date, few studies focusing on the presence and...
Tipo: Text Palavras-chave: STEC; EPEC; Coquillages; Diversité; Virulence; Persistance; STEC; EPEC; Shellfish; Diversity; Virulence; Persistence.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00312/42322/41653.pdf
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Produção de biomassa em Brachiaria decumbes com níveis deferenciados de manejo. Repositório Alice
BATISTA, L. A. R.; GODOY, R..
Com objetivo de elevar a produtividade das pastagens de Brachiaria decumbens, foi utilizado manejo com o uso de corretivos no preparo do solo e aplicação de fertilizantes no plantio e em cobertura após cada corte baseada na produtividade do corte, e irrigação suplementar (N1). Esses tratamentos foram contrastados com tratamentos que receberam somente corretivos e metade da adubação de recuperação no plantio, nenhuma adubação de cobertura e nenhuma irrigação suplementar (N2). O delineamento experimental foi o de blocos subdivididos no espaço e no tempo, com duas repetições. A produção de matéria seca foi avaliada por três anos, com diferentes intervalos entre cortes. Em média, N1 apresentou PBS 373% superior à produção de N2. A PBS acumulada no ano foi de...
Tipo: Separatas Palavras-chave: Pastagem tropical; Persistência; Fertilization; Persistance; Tropical pasture.; Adubação; Forragem; Irrigação.; Forage; Irrigation..
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/46116
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Recherche par PCR d'OsHV-1 (Ostreid Herpesvirus type 1), dans des échantillons d'eau de claires ostréicoles ArchiMer
Solliec, Gaelle.
Depuis 1991, des mortalités massives associées à des infections à herpèsvirus sont rapportées chez des larves et des juvéniles de différentes espèces de bivalves dans différentes régions du globe. Un nouveau groupe au sein de la famille des Herpesviridae a été créé afin d'y intégrer les herpèsvirus d'invertébrés marins avec actuellement pour unique représentant le virus infectant l'huître creuse, Crassostrea gigas, OsHV-1. Des études ont porté sur la mise en évidence d'OsHV-1 chez les coquillages d'intérêt économique après développement d'outils de diagnostic rapides et fiables. Ainsi, la maladie est aujourd'hui assez bien connue. Toutefois, le devenir d'OsHV-1 dans le milieu extérieur n'a fait l'objet que de peu d'études. Aussi, l'objectif principal de ce...
Tipo: Text Palavras-chave: Herpèsvirus; Bivalves; Huître creuse; OsHV-1; Eau de mer; Claire ostréicole; Persistance; Détection; Spécificité; Champignons marins; Isolement; Virus de type herpès.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00025/13644/10726.pdf
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การเคลื่อนย้ายและความคงทนของสารเคมีกำจัดวัชพืช กลุ่ม Amides ในพื้นที่ที่มีความลาดชันแตกต่างกัน ณ สถานีเกษตรหลวงอ่างขาง Thai Agricultural
Montri Toopornsiri.
Thesis (M.Sc. in Agriculture)
Tipo: PhysicalObject Palavras-chave: Herbicides; Amides; Persistance; Soil slopes; Royal Ang Khang Highland Agriculture Research Station; สารเคมีกำจัดวัชพืช; การเคลื่อนย้าย; ความคงทนต่อสาร; ผลตกค้าง; สารกลุ่มอะไมด์; พื้นที่ลาดชัน.
Ano: 1987 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5459
Registros recuperados: 8
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