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Aislamiento y caracterización bioquímica de metabolitos producidos por rizobacterias que solubilizan fosfato. Colegio de Postgraduados
Paredes Mendoza, Marianela.
El empleo de bacterias solubilizadoras de fosfato (BSP) para la solubilización de distintas rocas fosfatadas y de otras fuentes de fósforo es una alternativa para incrementar la cantidad de P disponible para las plantas. Se aislaron 147 BSP de la raíz y rizoplano de diferentes plantas cultivadas. El medio de cultivo denominado Medio Mínimo para Solubilización de Fosfato Colegio de Postgraduados con MgCl (MMSFCP-Mg) fue el más eficiente, debido a que en medio sólido se observaron halos de solubilización de hasta 3.8 cm a los catorce días, y en medio líquido se determinaron hasta 380 mg mL-1 de fosfato soluble a los seis días. Veintisiete de las mejores cepas fueron seleccionadas con base en la eficiencia de solubilización de fosfato...
Palavras-chave: Acidos orgánicos; Fosfatasas; Solubilización de fosfato; Inmunodetección; Roca fosfórica; Bacterias solubilizadoras de fosfato; Enfófitas; Organic acids; Phosphatases; Phosphate solubilization; Immunodetection; Rock phosphate; Phosphate solubilizing; Bacteria; Endophyte; Doctorado; Edafología.
Ano: 2010 URL: http://hdl.handle.net/10521/245
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Biochemical and cellularchanges in Oreochromis niloticus related to the water pollution of a degraded river - doi: 10.4025/actascibiolsci.v35i3.13207 Biological Sciences
Cruz, Zilma Maria Almeida; Universidade Vila Velha; Castro, Rodrigo Roque Lesqueves de; Universidade Vila Velha; Canal, Adriana; Azevedo Júnior, Romildo Rocha; Universidade Vila Velha; Silva, Ary Gomes da; Universidade Vila Velha; Ramos, Alessandro; Universidade Vila Velha.
The effects of polluted water at three sites in the Marinho River, Brazil, on Oreochromis niloticus (Nile tilápia) were investigated using histological, hematological and biochemical approaches. Fish exposed to the impacted water demonstrated that histological changes in gills were accompanied by nuclear and micronuclei abnormalities in cells. The activity of liver and plasma biomarkers (alkaline phosphatase (ALP), acid phosphatase (ACP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver glutathione S-transferase (GST)) showed an expressive change due to the. The results were also correlated with the highest levels of Cu+2, Zn+2 and Mn+2 in the water. The data of this study evidenced the importance of using a set of biomarkers to...
Palavras-chave: Aminotransferases; Phosphatases; Glutathione-S-transferase; Histology; Cell abnormality; Tilapia.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/13207
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Changes in phosphate content and phosphatase activities in rice seedlings exposed to arsenite Braz. J. Plant Physiol.
Mishra,Shruti; Dubey,R.S..
The effect of arsenite (As2O3) in situ on the level of the phosphate pool and activities of phosphohydrolytic enzymes was examined in rice (Oryza sativa L.) seedlings grown for 5-20 d in sand cultures. The effects were manifested via a decline in phosphate content and inhibition of the activities of key phosphatases. Application of 50 µM As2O3 in situ resulted in 34 to 77% inhibition of acid phosphatase activity in roots and about 38 to 50% inhibition of activity in shoots of 15-20-d-old seedlings. Similarly, alkaline phosphatase activity was inhibited in shoots under in situ As (III) toxicity. Varietal as well as organ specific differences were observed in the response of inorganic pyrophosphatase activity to in situ As (III) treatment. A moderately toxic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arsenite; Chloroplast; Mitochondria; Oryza sativa; Phosphate; Phosphatases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000100003
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Comparative toxicity of diflubenzuron and p-chloroaniline in phosphatases and antioxidant enzymes of on-target organisms. Repositório Alice
DANTZGER, D. D.; JONSSON, C. M.; AOYAMA, H..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Diflubenzuron; P-chloroaniline; Phosphatases; Antioxidant Enzymes.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/917714
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Copper influence on polyphosphate metabolism of Cunninghamella elegans BJM
Souza,Patrícia Mendes de; Marinho,Petrusk Homero; Lima,Marcos Antônio Barbosa de; Nascimento,Aline Elesbão do; Takaki,Galba Maria de Campos.
The aim of this work was to evaluate the physiological aspects of polyphosphate metabolism of Cunninghamella elegans grown in presence of copper. The growth profile was obtained by means of biomass yields, orthophosphate consumption, polyphosphate accumulation and phosphatases activities. The results revealed the influence of copper on the growth, observed by biomass yields. Orthophosphate consumption was faster in cells grown in the presence of copper. The presence of copper in the culture medium induced polyphosphate accumulation. The polyphosphate level was almost constant in the beginning of control culture growth, and could be related to the exponential growth phase. On the other hand, the copper treated cultures exhibited a significant reduction in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cunninghamella elegans; Copper; Polyphosphate; Phosphatases.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400002
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Dynamique du phosphore et rôle comme facteur limitant de la production planctonique dans le panache de la Gironde sur le plateau continental Sud Gascogne ArchiMer
Labry, Claire.
This study was supported by the Atlantic working site of the « Programme National d’Océanographie Côtière » (PNOC, PNEC since 1999). The objectives were (1) to check the existence and to understand the mechanisms of winter phytoplankton blooms in the bay of Biscay influenced by the Gironde waters (2) to determine the limiting nutrient of the primary production associated to these blooms and to follow the seasonal evolution of the limiting nutrient, (3) to understand the consequences of this early limitation on the structuration of algal populations in spring. Since phosphorus was supposed to be the major limiting nutrient of algal growth in the Gironde plume, the different forms of the phosphorus cycle as well as the bacterial compartment, central in this...
Tipo: Text Palavras-chave: Golfe de Gascogne; Gironde; Phytoplancton; Bactéries; Phosphates; Phosphore organique; Dissous; Phosphatases; Bay of Biscay; Gironde; Phytoplankton; Bacteria; Phosphate; Dissolved organic phosphorus; Phosphatases.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00013/12463/9298.pdf
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Evaluation of the toxicity of the mixture of diflubenzuron and p-chloroaniline in tilapia fish in the presence and absence of soil. Repositório Alice
DANTZGER, D. D.; JONSSON, C. M.; NUNES, A. A. S.; AOYAMA, H..
aBSTRACT: Several chemicals occur together in the environment as a result of different sources. However, in the great majority of risk assessments only a single chemical is considered. Diflubenzuron (DFB), the most used compound to combat parasites in aquaculture, can be toxic for non-target species. When degraded, it generates p-chloroaniline (PCA), a potentially carcinogenic and mutagenic compound. This study analyzed the activity of the enzymes: Catalase (CAT), acid and alkaline Phosphatase (ACP/ALP) of the gills of the Tilapia fish (Oreochromis niloticus) exposed to different concentrations of the mixture of DFB and PCA in the presence and absence of soil.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: P-chloroaniline; Phosphatases; Soil; Aquicultura; Agrotóxico; Impacto ambiental; Toxidez; Tilápia nilótica; Aquaculture; Diflubenzuron; Catalase; Antiparasitic agents; Ecotoxicology.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1064401
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Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010
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Phosphatases and antioxidant enzymes as potential biochemical indicators of toxicity in aquatic non-target organisms. Repositório Alice
DANTZGER, D. D.; JONSSON, C. M.; AOYAMA, H..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Phosphatases; Antioxidant Enzymes; Biochemical Indicators; Antioxidants; Enzymes; Diflubenzuron.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010917
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Presence of multiple acid phosphatases activity in seedlings of cucumber, radish and rocket salad Ciência Rural
Tabaldi,Luciane Almeri; Ruppenthal,Raquel; Pereira,Luciane Belmonte; Cargnelutti,Denise; Gonçalves,Jamile Fabbrin; Morsch,Vera Maria; Schetinger,Maria Rosa Chitolina.
Acid phosphatases (3.1.3.2) are a group of enzymes widely distributed in nature, which catalyze the hydrolysis of a variety of phosphate esters in the pH range of 4-6. We confirmed the presence of acid phosphatases in seedlings of cucumber (Cucumis sativus), radish (Raphanus sativus) and rocket salad (Eruca vesicaria) under different assay conditions using a rapid and simple preparation. The results showed that the optimum pH and temperature used for all species were close to 5.5 and 35°C, respectively. The enzyme was inhibited by molybdate, fluoride, azide, levamisole, orthovanadate, Zn2+ and Cu2+. Suramin had no effect on enzyme activity. The acid phosphatase from cucumber, radish and rocket salad hydrolyzed a wide variety of phosphate esters and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucumis sativus; Raphanus sativus; Eruca vesicaria; Phosphatases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300009
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