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Histological assessment of the phytohemagglutinin (PHA) skin test as a method to evaluate inflammatory response and cell mediated immunity in Magellanic penguins (Spheniscus magellanicus) 195
Ruoppolo, V.; Ribeiro, J. D.; Vanstreels, R. E. T.; Hurtado, R.; Pedron, B.; Cortopassi, S. R. G.; Catão-Dias, J. L..
To quantify cell-mediated immunity in immunoecology the phytohaemagglutinin-induced skin-swelling test is one of the most employed assays, especially for wild birds. The PHA induced skin swelling is the most widely used method to measure cell-mediated immunocompetence in birds. The objective of this study was to contribute to understanding the factors that modulate susceptibility, pathogenesis and severity of infectious diseases and inflammatory response in Magellanic penguins through evaluation of an immunological test that can measure cellular responses.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytohemagglutinin; Penguins phytohemagglutinin; Penguins.
Ano: 2016 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31052
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Isolation and partial characterization of a lectin from Bauhinia pentandra (bong) vog. Ex. Steua. 93
SILVA,ANDRÉ LUIS COELHO DA; HORTA,ANA CECÍLIA GÓES; MOREIRA,RENATO DE AZEVEDO.
Bauhinia pentandra (Bong) Vog. ex. Steua seeds were investigated with respect to phenologic aspects (size, mass, hilum and length) and with respect to their chemical composition. The total nitrogen content of the seed flour was determined, and the flour was extracted in different pH values. A lectin was isolated from the seeds by Sepharose-4B affinity chromatography. The homogeneity of the lectin was demonstrated by SDS-PAGE in the presence of beta-mercaptoethanol. Only one protein band with an apparent molecular mass of 30 kDa was found. The B. pentandra lectin showed a carbohydrate specificity for D-galactose, a requirement for divalent metal cations (Ca2+ and Mn2+) for full activity and amino acid composition with a high content of aspartic acid,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bauhinia; Leguminosae; Phytohemagglutinin; Seed protein; D-galactose.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000300002
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