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Controle ecológico de mutucas (Tabanidae sp.): experiência no Vale do Ribeira. Infoteca-e
MARCHI, G.; MARCHI, P..
bitstream/item/75747/1/art-005.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Inseto; Praga; Insecta; Plague.
Ano: 2008 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/571710
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The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA BJMBR
Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B..
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013
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Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA BJM
Feng,Na; Zhou,Yazhou; Fan,Yanxiao; Bi,Yujing; Yang,Ruifu; Zhou,Yusen; Wang,Xiaoyi.
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23 CFU for pure culture, whereas 2.3 × 104 or 2.3 × 106 CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3 × 106 CFU, but PCR was negative at the level of 2.3 × 107 CFU. After simulated spleen and lung samples were treated with magnetic beads, the sensitivity of LAMP or PCR was 2.3 × 103 or 2.3 × 106 CFU, whereas 2.3 × 105 or 2.3 × 107 CFU for magnetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plague; Yersinia pestis; Loop-mediated isothermal amplification; Magnetic beads.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100128
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