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Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States PAB
Botelho,Stephanie Regina Alves; Duarte,Macária Ferreira; Barbosa,Andreza Viveiros; Lau,Douglas; Sanches,Márcio Martinello; Fernandes,Fernanda Rausch.
Abstract: The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real-time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Exotic pest; High plains virus; Plant quarantine; Phytosanitary analysis..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500688
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Population dynamics of Pseudomonas savastanoi pv. phaseolicola in bean, throughout the epiphytic and pathogenic phases PAB
Marques,Abi Soares dos Anjos; Samson,Régine.
Abstract: The objective of this work was to monitor traits of the life cycle of Pseudomonas savastanoi pv. phaseolicola, in order to better understand the outbreak of bean halo blight, originating from a bacterial population in asymptomatic plants. Five experiments were conducted in the field, in greenhouses, and in humidity chambers. Changes in population size were evaluated in three field plantings, by introducing the bacteria in contamination focal points and observing the weather conditions favoring an outbreak. The dispersion of the bacteria in the field was followed by isolation and Bio-PCR analysis. Two assays were conducted in greenhouses and humidity chambers to evaluate the effect of leaf age on disease expression and the relationship between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Bio-PCR; Epidemiology; Halo blight; Plant quarantine; Weather conditions..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500623
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