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| Registros recuperados: 13 | |
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| Erisen,Semiha; Yorgancilar,Mustafa; Atalay,Emine; Babaoglu,Mehmet; Duran,Ahmet. |
| A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [?-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was... |
| Tipo: Journal article |
Palavras-chave: Astragalus; Callus; Organogenesis; Plant regeneration. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600013 |
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| Mercier,Helenice; Souza,Beatriz Maia; Kraus,Jane Elizabeth; Hamasaki,Regina Mayumi; Sotta,Bruno. |
| The in vitro culture of pineapple leaves on a shoot induction medium (SIM) results in the formation of protuberances and further development in shoots, and plantlets. The contents of endogenous indoleacetic acid (IAA) and five cytokinins (Cks), N6(2-isopentenyl)adenine (iP), N6(2-isopentenyl)adenosine (iPR), zeatin (Z), zeatin riboside (ZR) and N6-benzyladenine (BA), present in the basal portion of those leaves, were correlated to the organogenic response that occurs over 15 days of culture. The endogenous auxin/cytokinins ratio was lowest on the 3rd day, mainly due to a strong increase in the iP level. It seems that endogenous iP concentration triggered the induction signal for an organogenic response in pineapple leaf bases. The rise in iP content... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ananas comosus; Auxin; Bromeliaceae; Cytokinin; Hormone signalling; Plant regeneration. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200006 |
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| MONTENEGRO,GLORIA; GINOCCHIO,ROSANNA; SEGURA,ALEJANDRO; KEELY,JOHN E.; GÓMEZ,MIGUEL. |
| Wildfires resulting from thunderstorms are common in some Mediterranean-climate regions, such as southern California, and have played an important role in the ecology and evolution of the flora. Mediterranean-climate regions are major centers for human population and thus anthropogenic impacts on fire regimes may have important consequences on these plant formations. However, changes in fire regimes may have different impacts on Mediterranean type-ecosystems depending on the capability of plants to respond to such perturbations. Therefore, we compare here fire regimes and vegetation responses of two Mediterranean-climate regions which differ in wildfire regimes and history of human occupation, the central zone of Chile (matorral) and the southern area of... |
| Tipo: Journal article |
Palavras-chave: Human impacts; Plant regeneration; Matorral; Chaparral. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2004000300005 |
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| Abdullah,Ruslan; Zainal,Alizah; Yew Heng,Wee; Chui Li,Leaw; Chee Beng,Yeap; Mei Phing,Lee; Abdullah Sirajuddin,Salwa; Soo Ping,Winnie Yap; Lourdes Joseph,Juanita; Azma Jusoh,Siti. |
| Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation... |
| Tipo: Journal article |
Palavras-chave: Genetic transformation studies; Immature embryos; In vitro culture; Oil palm; Plant regeneration. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006 |
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| Bakar,Dalilah Abu; Ahmed,Bakrudeen Ali; Taha,Rosna Mat. |
| Celosia argentea (Var.) cristata (Amaranthaceae) is a widely cultivated ornamental plant, which has antibacterial, astringent, haemostatic, hypertensive, ophthalmic, and parasitic significance. This study describes a protocol for in vitro callus induction and plant regeneration from leaf and stem explants of C. argentea using Murashige and Skoog (MS) medium. Callus culture was initiated and established from seedling, leaf, and stem explants. Explants were cultured on MS medium supplemented with auxin alone (0.5 mg/L Naphthaleneacetic acid (NAA), 2, 4-Dicholorophenoxyacetic acid (2, 4-D). Green and red compact callus (98%) were induced using MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L Benzyladenine (BA). Two different concentrations (1.0 mg/L BA +... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Celosia argentea; Amaranthaceae; In vitro; Micropropagation; Callus; Plant regeneration. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600860 |
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| Carvalho,Paula Pinheiro de; Antoniazzi,Camila Aparecida; Faria,Rodrigo Brito de; Carvalho,Ilio Fealho de; Rocha,Diego Ismael; Silva,Maurecilne Lemes da. |
| ABSTRACT The present study reports a shoot organogenesis-based system for in vitro regeneration of Passiflora miniata, an Amazonia passion fruit species. Root segments were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations (range 2-9 µM) of 6-benzyladenine (BA); thidiazuron (TDZ) or kinetin (KIN). Plant growth regulators were not added to the control treatment. Root explants have showed a high regenerative potential. After 30 days of in vitro culture, the root explants showed several shoots formed direct and indirectly. TDZ provided the best response in the differentiation adventitious shoots, mainly in the presence of 6.8 µM. The cytokinins BA and KIN responded producing a reduced number of shoots. After 120 days,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Morphogenesis; Ornamental passion fruit; Plant regeneration; Root explant; Shoot organogenesis; Thidiazuron. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100406 |
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| Pesqueira,Julieta; García,Maria Dina; Staltari,Sebastian; Molina,Maria del Carmen. |
| High salt concentrations in soils negatively affect maize growth. Techniques such as remote hybridization and in vitro selection have been extensively used to accelerate breeding processes. In order to determine the usefulness of Tripsacum to improve salt tolerance in maize, the effects of NaCl, in vitro and in vivo, were evaluated in an intergeneric hybrid (MT) obtained from crossing Zea mays with Tripsacum dactyloides. Organogenic calli, induced from immature MT hybrid embryos, were exposed to different NaCl concentrations and the survival and regeneration percentages were calculated. Plants of the MT hybrid, obtained from the organogenic calli, were exposed to NaCl concentrations considered harmful for maize. The shoot dry weights of plants exposed to... |
| Tipo: Journal article |
Palavras-chave: Intergeneric hybrid; Plant regeneration; Organogenesis; Salinity tolerance. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300022 |
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| Ramírez-Villalobos,Maribel; de García,Eva. |
| The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, were solid media supplemented with (mg L-1) 22.7 BA plus 0.192 IAA, and 25 BA plus 0.217 IAA, respectively, all containing 1.8 g L-1 of phytagel, and subcultures were performed monthly and bimonthly over 16 months. The other two treatments, IT22 and IT25, resembled ME22 and ME25 but consisted in temporary immersion for four months without subcultures, followed by two months in solid media. The scalps were grown in callus-induction medium and embryogenic calluses... |
| Tipo: Journal article |
Palavras-chave: Bananas; Embryogenic callus; Embryogenic cell suspension; Musa sp; Plant regeneration; Scalp; Secondary somatic embryogenesis. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500006 |
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| Gharehmatrossian,Sormeh; Popov,Yu; Ghorbanli,Mahlagha; Safaeian,Shila; Iranbakhsh,Alireza. |
| ABSTRACT Shoot regeneration, callus growth, and biosynthesis of shikonin in callus cultures of Onosma sericeum were examined. Plant tissue culture was used as an alternative method for increasing the production of shikonin, a secondary metabolite. The isolated cultures were subjected to abiotic factors such as light, plant growth regulators, and nutritional factors. Identification was carried out by High- Performance Liquid Chromatography (HPLC) after 10th subculture. Nodal explants were incubated in Murashige and Skoog (MS) medium along with different combination of growth hormones. Shoot regeneration from calli were achieved on MS basal medium supplemented with 3 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l Naphthalene acetic acid (NAA) under light cycle.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth index; Medicinal plant; Plant regeneration; Secondary metabolite. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100428 |
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| ÇÖLGEÇEN,HATICE; TOKER,M CIHAT. |
| The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm-3 BAP and 1 mg dm-3 NAA. 94.4% of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm-3 NAA. In vitro organogénesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field |
| Tipo: Journal article |
Palavras-chave: Red clover (Trifolium pratense L.); Fabaceae; Organogenesis; Plant regeneration. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100004 |
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| Registros recuperados: 13 | |
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