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| Petrillo,Caroline Pereira; Carneiro,Newton Portilho; Purcino,Antônio Álvaro Corsetti; Carvalho,Carlos Henrique Siqueira; Alves,José Donizeti; Carneiro,Andréa Almeida. |
| The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm. Transformation frequencies obtained using these conditions ranged from 0.9 to 2.31%. Integration of foreign genes into the genome of maize plants was confirmed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Ammonium glufosinate; Beta-glucuronidase; Immature embryos; Phosphinotricin acetyl transferase; Plant transformation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000300012 |
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| Alves,Adriana Cristina; Quecini,Vera Maria; Vieira,Maria Lucia Carneiro. |
| Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation), screening (reporter gene possibilities and performance) and transformant selection (ipt selective marker) are discussed. Transgene expression and mechanisms underlying (trans)gene inactivation are presented.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant transformation; Agrobiolistics; Electroporation; Reporter genes; Transgenic crops. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000100001 |
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| FREITAS-ASTÚA,JULIANA; PURCIFULL,DAN E.; POLSTON,JANE E.; HIEBERT,ERNEST. |
| Viruses of to the family Geminiviridae are considered some of the most important pathogens in tropical and subtropical regions of the world. Members of one Geminiviridae genus, Begomovirus, have been causing severe losses, particularly in tomato (Lycopersicon esculentum) production in the Americas and the Caribbean. Several new begomoviruses have been reported in the region and, at least one, Tomato yellow leaf curl virus (TYLCV), has been brought in from the Old World via infected transplants. In addition, the recombination events that are playing an important role in Begomovirus diversity have increased the complexity of their control. This scenario has led to the search for control measures that go beyond traditional host genetic resistance, chemical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Whitefly-transmitted geminivirus; Gene silencing; Plant transformation; Tomato begomovirus; Geminivirus control; Geminiviridae. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000500001 |
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