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Registros recuperados: 27
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AN IN VITRO STUDY OF THE EFFECTS OF VENOM OF AUSTRALIAN ELAPIDS ON MURINE SKELETAL MUSCLE AND THE PROTECTIVE EFFECT OF HOMOLOGOUS PLASMA J. Venom. Anim. Toxins
BUTLER,T. L.; JACOBSEN,P. F.; MIRTSCHIN,P. J.; KAKULAS,B. A..
The venom of many dangerous Australian snakes has a myotoxic component and some are strongly myolytic. The myotoxicity of venom of seven Australian elapid snakes was studied to determine their relative in vitro potency in causing cell death of C2C12 cells, a myoblast cell line, and murine myotubes in mixed cell culture. The venom of Pseudechis australis proved to be the most myotoxic, Austrelaps superbus and Pseudechis porphyriacus venoms also exhibited high myotoxicity relative to the other venoms tested. The specificity of Pseudechis porphyriacus venom was tested using the human glioma cell line TC3 and was shown to exhibit a general cytotoxicity. Myotoxicity, however, was the predominant action of the venom. It has long been known that certain animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom antidote; Plasma; Myotoxicity; Murine skeletal muscle; Australian elapids.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100004
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Balanço de nitrogênio e estimativas de perdas endógenas em vacas lactantes alimentadas com dietas contendo palma forrageira e teores crescentes de uréia e mandioca - DOI: 10.4025/actascianimsci.v28i1.848 Animal Sciences
Soares Cruz, Maria Cláudia; CEFET-Petrolina; Véras, Antonia Sherlânea Chaves; UFRPE; Ferreira, Marcelo de Andrade; UFRPE; Batista, Angela Maria Vieira; UFRPE; Santos, Djalma Cordeiro dos; IPA; Coelho, Marcelo Iran de Souza; CEFET-Pernambuco.
Foram avaliados os efeitos da substituição do farelo de soja pela mistura uréia mais mandioca, em rações constituídas de palma, silagem de sorgo e concentrado, sobre concentrações de uréia, balanço de nitrogênio (N) e estimativas de perdas endógenas totais em 8 vacas Girolando em lactação, distribuídas em dois quadrados latinos simultâneos (4x4). As concentrações de uréia e N-uréico no plasma e leite, em mg/dL e N-uréico no leite (g/dia) não foram afetadas pelo aumento do nível de uréia na ração, apresentando médias de 31,37; 14,62; 37,43; 17,44; 5,37. As excreções urinárias de uréia e N-uréico também não foram influenciadas, com médias de 249,45 e 116,24 mg/kg PV. Igualmente, o balanço de N não foi afetado pelos tratamentos, cujos valores médios foram de...
Palavras-chave: 5.04.00.00-2 Zootecnia bovinos; Leite; Plasma; Uréia; Urina. 5.04.00.00-2 Zootecnia.
Ano: 2006 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/848
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Cálcio, fósforo e proteína total no sangue de frangos de corte em função de níveis de balanço eletrolítico da ração Arq. Bras. Med. Vet. Zootec.
Vieites,F.M.; Fraga,A.L.; Moraes,G.H.K.; Vargas Júnior,J.G.; Nalon,R.P.; Corrêa,G.S.S.; Nunes,R.V..
Determinaram-se os melhores valores do balanço eletrolítico (BE) de dietas para frangos quanto aos teores de cálcio, fósforo e proteína total no sangue de frangos de corte aos 42 dias de idade. Foram utilizadas 1728 aves de marca comercial, machos. As aves foram alimentadas com duas dietas basais, 20 ou 23% de proteína bruta (PB), na fase inicial, e uma única ração basal, 20% de PB, na fase de crescimento, combinadas com níveis de balanço eletrolítico de 0; 50; 100; 150; 200; 250; 300 e 350mEq/kg. Foi utilizado delineamento experimental inteiramente ao acaso, em arranjo fatorial, com seis repetições de 18 aves cada e 96 unidades experimentais. Foi escolhida uma ave em cada unidade experimental para a coleta de sangue. Foram avaliados os teores de cálcio,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicultura industrial; Equilíbrio eletrolítico; Homeostasia; Minerais; Plasma.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400014
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Changes of Progesterone and Endothelin Concentrations in the Peripheral Plasma of Female Calves and Cycling Cows: Effects of PGF2α Injection OAK
OHTANI, Masayuki; OKI, Naoko; TANIMURA, Miyune; KOBAYASHI, Shu-ichi; ACOSTA, Tomas J.; HAYASHI, Kanako; MIYAMOTO, Akio; 宮本, 明夫.
It is well known that an injection of prostaglandin F2α (PGF2α) or its analogue during the mid luteal phase of the estrous cycle induces a rapid decrease in plasma progesterone (P4) concentration, followed by luteolysis in the cow. There is evidence that a potent vasoactive peptide, endothelin-1 (ET-1), is produced in the bovine corpus luteum (CL), and that it is directly involved in luteolysis. We previously found that ET-1 concentrations in the peripheral plasma increase during the period of luteolysis and ovulation in cows. However, it is not clear whether the elevation of peripheral plasma ET-1 concentration observed during luteolysis and ovulation originates exclusively from the ovary and/or CL. Such a profile of plasma ET-1 concentration may be...
Palavras-chave: Endothelin; Progesterone; Plasma; Calves; Cows.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2973
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Characterization of innate immune activity in Phrynops geoffroanus (Testudines: Chelidae) Rev. Bras. Zool.
Ferronato,Bruno O.; Merchant,Mark E.; Marques,Thiago S.; Verdade,Luciano M..
The innate immune activity of the freshwater turtle Phrynops geoffroanus (Schweigger, 1812) was investigated, using a sheep-red-blood cell hemolysis assay. The time- and concentration-dependent hemolytic activity of the turtle plasma was low compared to that reported for other reptiles. However the plasma of P. geoffroanus exhibited higher activity at elevated temperatures, resulting in temperature-dependent hemolysis. The sensitivity of turtle plasma to temperature could be interpreted as a mechanism by which freshwater turtles use basking behavior to elevate body temperature, thus enhancing the innate immune response. However, we cannot discard the possibility that environmental contaminants could be affecting the turtle's immune response, since the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chelonian; Immunology; Plasma.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000400020
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Chromosome damage in underground coal miners: detection by conventional cytogenetic techniques and by submitting lymphocytes of unexposed individuals to plasma from at-risk groups Braz. J. Genet.
Agostini,J.M.S.; Otto,P.A.; Wajntal,A..
Chromosome abnormalities and the mitotic index in lymphocyte cultures and micronuclei in buccal mucosa cells were investigated in a sample of underground mineral coal miners from Southern Brazil. A decreased mitotic index, an excess of micronuclei and a higher frequency of chromosome abnormalities (fragments, polyploidy and overall chromosome alterations) were observed in the miners when compared to age-paired normal controls from the same area. An alternative assay for clastogenesis in occupational exposition was tested by submitting lymphocytes from non-exposed individuals to a pool of plasmas from the exposed population. This assay proved to be very convenient, as the lymphocytes obtained from the same individuals can be used as target as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome damage; Underground coal miners; Cytogenetic; Lymphocytes; Plasma; At-risk groups.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400021
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Comparison of the quantification of caffeine in human plasma by gas chromatography and ELISA BJMBR
Carregaro,A.B.; Woods,W.E.; Tobin,T.; Queiroz-Neto,A..
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according to the criterion of difference, in modulus, of the I50 of the standard curve and of the I50 of the sample. The samples were classified into three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Gas chromatography; ELISA; Caffeine; Plasma; Quantification.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600019
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Concentração iônica plasmática e biliar do "curimbatá" Prochilodus scrofa Steindachner (Osteichthyes, Characiformes) em relação aos estádios de repouso e maduro do ciclo reprodutivo Zoologia
Moreira,Renata Guimarães; Mimura,Olga Martins; Lima,Rossana L.V. Andrade; Bernardino,Geraldo.
Body fluid ionic content of fishes varies with ambient. Variations in the plasmatic ionic concentration have also been related with sex or maturation cycle, once that reproduction modifies metabolic processes. In fishes, the gallbladder is an important osmoregulatory organ. So, the present work has been focused on the relation of plasmatic and biliar electrolytes with sex and maturation cycle, of the teleost fish Prochilodus scrofa at the resting and mature stages of gonadal cycle. The animals (28 males and 30 females) were collected at Cachoeira de Emas (Pirassununga, São Paulo) and they were fasted for three days before the experiments. Deteminations of Na+, K+, Mg++, and Ca++ were made by spectophotometry and Cl- by microtitulation. Comparisons of ionic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteichthyes; Characiformes; Prochilodus scrofa; Bile; Plasma; Ionic content.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000100019
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Determination of Gemifloxacin in human plasma by high performance liquid chromatography using Ultra Violet detector and its application to a bioequivalence study BJPS
Mousavi,Syed Husain Hashemi.
A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gemifloxacin/determination; Bioequivalence; Plasma; HPLC.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400610
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Development of a new HPLC method for in vitro and in vivo studies of haloperidol in solid lipid nanoparticles BJPS
Yasir,Mohd; Sara,Udai Vir Singh; Som,Iti.
ABSTRACT A simple and sensitive HPLC method was developed and validated for the quantification of haloperidol in solid lipid nanoparticles (SLNs). The developed method was used for detection of shelf life of haloperidol in SLNs. Calibration curve of haloperidol was also constructed in rat plasma using loratidine as internal standard. In vivo studies were performed on rats and concentration of haloperidol in brain and blood was measured for the determination of various pharmacokinetic and hence brain targeting parameters. Chromatogram separation was achieved using C18 column as stationary phase. The mobile phase consisted of 100 mM/L potassium dihydrogen phosphate-acetonitrile-TEA (10:90:0.1, v/v/v) and the pH was adjusted with o-phosphoric acid to 3.5....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haloperidol/quantification; Haloperidol/pharmacokinetic; Haloperidol/in vitro study; Haloperidol/in vivo study; High performance liquid chromatography/solid lipid nanoparticles; Plasma; Validation; Brain targeting..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200623
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Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents BABT
Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p<0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035
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Effects of diets with increasing levels of citrus pulp on the blood parameters linked to energy metabolism in horses Ciência e Agrotecnologia
Menezes,Madalena Lima; Brandi,Roberta Ariboni; Bueno,Ives Cláudio da Silva; Balieiro,Júlio Cesar de Carvalho; Moreira,Camilla Garcia; Nascimento,Olivia Carmen de Araújo.
Currently, the use of alternative energy ingredients for horses has increased because these foods contain "superfibers", making safer diet for these animals. To study the influence of diets containing increasing levels of citrus pulp on albumin, triglycerides, cholesterol, glucose, insulin and short chain fatty acid (SCFA) concentrations in the blood, five animals were used, with an average age of 3.5 years and a live weight of 460.66±76.86 kg, they were fed twice a day, at 7:00 am and 4:00 pm. The diets were formulated to meet the requirements of the animals at maintenance. 60% of the energy was obtained from forage, and 40% from concentrate, containing the following inclusion levels of citrus pulp (0, 7, 14, 21 and 28%). The area under the curve (AUC)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soluble fiber; Nutrition; Pectin; Plasma.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000600008
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Eletroforese das proteínas plasmáticas em emas (Rhea americana) de diferentes faixas etárias Ciência Rural
Conrado,Alexandre de Carvalho; Lopes,Sonia Terezinha dos Anjos; Martins,Danieli Brolo; Duarte,Marta Frescura; Mortari,Antonio Carlos; Flores,Maristela Lovato; Barasuól,Luciane.
A rheacultura vem crescendo em todo o Brasil. Porém, ainda há uma escassez de informações sobre esta espécie e de médicos veterinários especializados na área. Este trabalho teve como objetivo verificar as diferenças existentes na eletroforese plasmática (gel de agarose) em idades distintas de emas (Rhea americana). Utilizaram-se 45 emas, separadas em quatro grupos: grupo 1 (n=10), animais com 15 dias de idade; grupo 2 (n=10), animais com 30 dias; grupo 3 (n=10), animais com 45 dias e grupo 4 (n=15), animais com 1 ano. Verificou-se homogeneidade nos valores eletroforéticos analisados nas aves dentro de cada faixa etária. Houve diferença entre grupos etários em valores de proteína plasmática total, albumina, globulinas e relação albumina/globulinas. Também...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eletroforese; Plasma; Rhea americana; Idade.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400018
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Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens BJMBR
Veloso,D..
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of ~65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178 <FONT FACE="Symbol">®</FONT> stop) has been blamed for the absence of LK and HK. However, using Western blots...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kininogen deficiency; Kinins; Antibodies; Plasma; Kininogen-like species; Human and nonhuman primates.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000700004
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Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy &amp; Gaimard, 1824 and) Leporinus obtusidens (Valenciennes, 1837) Neotropical Ichthyology
Loro,Vania Lucia; Glusczak,Lissandra; Moraes,Bibiana S.; Leal,Claudio A. M.; Menezes,Charlene; Murussi,Camila R.; Leitemperger,Jossiele; Schetinger,Maria Rosa C.; Morsch,Vera Maria.
Rhamdia quelen (silver catfish) and Leporinus obtusidens (piava) were exposed to a commercial formulation Roundup(r), a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase) activities. Plasmatic glucose levels were reduced in both species, whereas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Mucus; Nucleotidases; Plasma; Protein carbonyl.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100229
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Hidrolisado de mucosa intestinal de suínos em substituição ao plasma sangüíneo em dietas para leitões de 21 a 49 dias R. Bras. Zootec.
Corassa,Anderson; Lopes,Darci Clementino; Pena,Sérgio de Miranda; Freitas,Letícia Silva de; Pena,Graciane de Miranda.
Um experimento foi conduzido para avaliar o efeito da substituição parcial ou total do plasma sangüíneo por hidrolisado de mucosa intestinal de suínos (HI) em dietas para leitões de 21 a 49 dias. Cento e quarenta leitões desmamados aos 21,7 ± 2,9 dias com peso médio de 5,54 ± 0,65 kg foram distribuídos em um delineamento experimental de blocos ao acaso, com cinco tratamentos, sete repetições e quatro animais por unidade experimental. O experimento foi dividido nas fases de 21 a 35 dias e de 36 a 49 dias de idade. Os tratamentos consistiram de uma dieta com plasma e de quatro dietas contendo diferentes níveis dos produtos HI 50 e HI 62 em substituição ao plasma. A substituição parcial ou total do plasma pelos produtos HI 50 e HI 62 não resultou em...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hidrolisado de mucosa intestinal de suínos; Leitões; Morfologia intestinal; Plasma.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007000900012
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Metabolismo de nitrogênio em novilhas alimentadas com dietas contendo cana-de-açúcar tratada com óxido de cálcio R. Bras. Zootec.
Carvalho,Gleidson Giordano Pinto de; Garcia,Rasmo; Pires,Aureliano José Vieira; Detmann,Edenio; Silva,Robério Rodrigues; Pereira,Mara Lúcia Albuquerque; Santos,Alana Batista dos; Pereira,Taiala Cristina de Jesus.
Esta pesquisa foi realizada para avaliar o balanço de nitrogênio, as concentrações de ureia na urina e no plasma e a síntese de proteína microbiana em novilhas alimentadas com dietas contendo cana-de-açúcar tratada com óxido de cálcio. Utilizaram-se 20 novilhas mestiças Holandês-Zebu com peso corporal médio inicial de 200 kg distribuídas em delineamento inteiramente ao acaso, com quatro tratamentos e cinco repetições. Os animais foram alojados em baias individuais cobertas, com piso de concreto, providas de cocho individual de concreto para alimentação e bebedouro automático comum a duas baias. O período experimental foi de 21 dias, e a coleta de dados foi realizada nos sete dias finais. As dietas foram formuladas para ser isoproteicas, com 14% de proteína...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cal; Plasma; Purinas microbianas; Retenção de nitrogênio.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000300022
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Níveis de fósforo para caprinos: perda endógena fecal e exigência líquida para mantença PAB
Bueno,Mauro Sartori; Vitti,Dorinha Miriam Silber Schmidt.
Foram utilizados, no experimento, nove caprinos machos castrados, da raça Alpina, em dois períodos. Os animais permaneceram 28 dias em dieta experimental composta de feno e concentrado, sem suplementação de P, ou com 1g ou 2 g de P, na forma de fosfato bicálcico. No 21º dia os animais receberam injeção de 7,4 MBq de 32P na jugular, e coletaram-se amostras de sangue, fezes e urina, por sete dias, com o objetivo de avaliar o metabolismo do P. O aumento do consumo de P levou a aumento linear significativo do P excretado nas fezes, do P absorvido, do P endógeno fecal, da eficiência de absorção, e dos teores de P nas fezes e no plasma. O aumento do P endógeno fecal (Y) em função do consumo de P (X) pode ser descrito pela equação Y= 10,36 + 0,58X (r=0,94); a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fezes; Fósforo radioativo; Plasma; Saliva; Urina.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000400020
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Oxidation of the albumin thiol to sulfenic acid and its implications in the intravascular compartment BJMBR
Turell,L.; Carballal,S.; Botti,H.; Radi,R.; Alvarez,B..
Human serum albumin (HSA) is the most abundant protein in the intravascular compartment. It possesses a single thiol, Cys34, which constitutes ~80% of the total thiols in plasma. This thiol is able to scavenge plasma oxidants. A central intermediate in this potential antioxidant activity of human serum albumin is sulfenic acid (HSA-SOH). Work from our laboratories has demonstrated the formation of a relatively stable sulfenic acid in albumin through complementary spectrophotometric and mass spectrometric approaches. Recently, we have been able to obtain quantitative data that allowed us to measure the rate constants of sulfenic acid reactions with molecules of analytical and biological interest. Kinetic considerations led us to conclude that the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thiol; Sulfhydryl; Albumin; Sulfenic acid; Mixed disulfides; Plasma.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400001
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Phytase in rations of growing pigs: performance, blood parameters and bone mineral content Ciência e Agrotecnologia
Silva,Hunaldo Oliveira; Fialho,Elias Tadeu; Freitas,Rilke Tadeu Fonseca de; Lima,José Augusto de Freitas; Logato,Priscila Rosa Vieira; Schoulten,Neudi Artemio.
It was intended with the present work to verify the effect of phytase on the performance, bone mineralization and content of plasma urea of swine. 80 swine (30 ± 3.1 kg/LW), crossbred (LD x LW) males and females, allocated to a randomized block design (RBD) with four treatments and five replicates. The treatments consisted of a growing swine diet on the basis of corn, soybean meal and defatted rice bran (DRB) supplemented with four levels of phytase (0, 400, 800 and 1200 FTU/kg) The diet was formulated to meet the requirements of growing swine except for available calcium and phosphorus, the levels of which were 0.64 and 0.25%, respectively. The enzyme phytase utilized was Natuphos 5000. Over the experimental period every eight days, blood of two animals...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme; Minerals; Plasma; Urea.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600028
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