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Registros recuperados: 8
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Comparison of haploid and doubled haploid sugar beet clones in their ability to micropropagate and regenerate Electron. J. Biotechnol.
Klimek-Chodacka,Magdalena; Baranski,Rafal.
Background: Haploid plant material is considered as recalcitrant to organogenesis, propagation, and maintenance in vitro. However, sugar beet (Beta vulgaris L.) breeders utilizing doubled haploid (DH) technology in their breeding programs indicate that sugar beet haploids may be cultured in vitro as well as diploids. Thus in this paper the in vitro performance of haploid and the doubled haploid sugar beet of various origin was evaluated. The DHs were derived from haploids by diploidization and twelve such haploid and corresponding DH clone pairs were obtained thus the comparison included haploid and DH clones that had identical allelic composition and differed only in their ploidy level. Results: The genotypes differed in shoot morphology and...
Tipo: Journal article Palavras-chave: Adventitious buds; Axillary shoots; Beta vulgaris; Organogenesis; Ploidy.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200001
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Effects of Alexandrium minutum exposure upon physiological and hematological variables of diploid and triploid oysters, Crassostrea gigas ArchiMer
Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Gueguen, Marielle; Moal, Jeanne; Palacios, Elena; Lassus, Patrick; Soudant, Philippe.
The effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon physiological parameters of the Pacific oyster, Crassostrea gigas, were assessed. Diploid and triploid oysters were exposed to cultured A. minutum and compared to control diploid and triploid oysters fed T. Isochrysis. Experiments were repeated twice, in April and mid-May 2007, to investigate effects of maturation stage on oyster responses to A. minutum exposure. Oyster maturation stage, Paralytic Shellfish Toxin (PST) accumulation, as well as several digestive gland and hematological variables, were assessed at the ends of the exposures. In both experiments, triploid oysters accumulated more PSTs (approximately twice) than diploid oysters. Significant...
Tipo: Text Palavras-chave: Oysters; Ploidy; Physiology; Harmful-algal bloom; Alexandrium minutum; PST accumulation.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11378/7988.pdf
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Herbage Production, Nutritive Value and Grazing Preference of Diploid and Tetraploid Perennial Ryegrass Cultivars (Lolium perenne L.) Chilean J. Agric. Res.
Balocchi,Oscar A; López,Ignacio F.
The objective of this study was to determine, under the soil and climatic conditions of Southern Chile, the effect of the ploidy of perennial ryegrass (Lolium perenne L.) cultivars on herbage production, nutritive value, grazing preference and utilization of pasture produced. This study was conducted in southern Chile, Valdivia Province, and was evaluated for 3 years. The tetraploid cultivars used were Quartet (4n), Gwendal (4n), Pastoral (4n) and Napoleon (4n). The diploid cultivars were Anita (2n), Jumbo (2n), Aries (2n), and Yatsyn 1 (2n).When the average sward height reached 20 cm, all plots were simultaneously grazed by dairy cows for a period of 24 h. Before and after grazing, sward height, dry matter availability and nutritive value were evaluated....
Tipo: Journal article Palavras-chave: Grazing preference; Herbage mass; Ploidy.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300005
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Ploidy level and caffeine content in leaves of Coffea Scientia Agricola
Silvarolla,Maria Bernadete; Mazzafera,Paulo; Lima,Marinez Muraro Alves de; Medina Filho,Herculano Penna; Fazuoli,Luiz Carlos.
Several species of Coffea and varieties of C. arabica differing in chromosome number had their caffeine content determined in the leaves. For Coffea arabica var. "Angustifolia", var. "Caturra" and var. "Icatu", it was observed caffeine decrease from the haploid (2n = 22) to tetraploid leaves (2n = 44). Caffeine in the tetraploid "Angustifolia" was decreased by 50% when compared to the haploid. Caffeine reduction was also observed in leaves of C. canephora var. "Kouilou" as chromosome number was increased (2n = 22 ® 2n = 44). In this case, caffeine in the leaves of the diploid genotype was close to 4 times higher than in the tetraploid. On the other hand it was observed an increase of the alkaloid when the chromosome number was doubled in the C. canephora...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caffeine; Coffea; Leaves; Ploidy.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000300021
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Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures BABT
Carone,Sílvia Bicudo; Santa-Catarina,Claudete; Silveira,Vanildo; Floh,Eny Iochevet Segal.
The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Nicotiana tabacum; Ploidy; Putrescine; Spermidine; Spermine.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020
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Single or dual experimental infections with Vibrio aestuarianus and OsHV-1 in diploid and triploid Crassostrea gigas at the spat, juvenile and adult stages ArchiMer
Azema, Patrick; Travers, Marie-agnes; Benabdelmouna, Abdellah; Degremont, Lionel.
French production of the Pacific cupped oyster, Crassostrea gigas, is currently threatened by two pathogens, OsHV-1 and V. aestuarianus. While oysters selected for their higher resistance to OsHV-1 are now available for the industry, the impact of V. aestuarianus on such oysters is unknown, especially for triploids. In addition, experimental infection has used the virus or the bacteria alone, but there have been no investigations of dual exposure to these pathogens. This study is the first report of single or dual exposure in spat (Spat1 and Spat2), juvenile and adult naïve oysters. For each of the two stocks evaluated, unselected oysters and oysters selected for their higher resistance to OsHV-1 infection were tested, as well as their triploid siblings of...
Tipo: Text Palavras-chave: Vibrio aestuarianus; OsHV-1; Dual exposure; Ploidy; Selection; Crassostrea gigas.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45835/45918.pdf
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Somaclonal variation in Asparagus officinalis plants regenerated by organogenesis from long-term callus cultures Genet. Mol. Biol.
Pontaroli,Ana Clara; Camadro,Elsa Lucila.
Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower morphology. Ploidy changes were observed in 37.8% of the 37 regenerants studied. Meiotic alterations in 12 out of 21 regenerants included laggards, dicentric bridges, micronuclei, restitution nuclei and polyads. Of the 408 AFLP markers screened in 43 regenerants and the donors, 2.94% showed polymorphism. High pollen viability was observed in the 22 regenerants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AFLP; Fertility; Plant morphology; Meiotic behavior; Ploidy.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300015
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The mass mortality of blue mussels (Mytilus spp) from the Atlantic coast of France is associated with heavy genomic abnormalities as evidenced by flow cytometry ArchiMer
Benabdelmouna, Abdellah; Ledu, Christophe.
Since 2014, France’s blue mussel industry has been facing heavy mortality outbreaks (90-100%) affecting both juveniles and adults. This report presents evidence of heavy genomic abnormalities associated with mortality outbreaks in blue mussels, Mytilus edulis-galloprovincialis, from the Atlantic coast of France. In this study, ploidy characteristics of hemic cells were investigated using Flow CytoMetry (FCM), revealing an unusual, broad continuum of ploidy distribution from hypodiploidy to tetraploidy. FCM was additionally used to evaluate, at individual and populations levels, different thresholds of genomic abnormality (GA%) using the percentage of non-diploid nuclei. Individual mussels were considered to be abnormal when more than 10% of hemocytes in...
Tipo: Text Palavras-chave: Mussels; Mortality; Cytogenetic quality; Hemocytes; Ploidy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00341/45171/44566.pdf
Registros recuperados: 8
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