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Registros recuperados: 9
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Agrowaste-based Polyhydroxyalkanoate (PHA) production using hydrolytic potential of Bacillus thuringiensis IAM 12077 BABT
Gowda,Vaishnavi; Shivakumar,Srividya.
The study identified the innate enzymatic potential (amylase) of the PHB producing strain B.thuringiensis IAM 12077 and explored the same for cost-effective production of PHB using agrowastes, eliminating the need for pretreatment (acid hydrolysis and/or commercial enzyme). Comparative polyhydroxyalkanoate (PHA) production by B. thuringiensis IAM 12077 in biphasic growth conditions using glucose and starch showed appreciable levels of growth (5.7 and 6.8 g/L) and PHA production (58.5 and 41.5%) with a PHA yield of 3.3 and 2.8 g/L, respectively. Nitrogen deficiency supported maximum PHA yield (2.46 g/L) and accumulation (53.3%). Maximum growth (3.6 g/L), PHB yield (2.6 g/L) and PHA accumulation (72.8%) was obtained with C:N ratio of 8:1 using starch as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis IAM 12077; Polyhydroxybutyrate; Nutrient limitation; Hydrolytic enzymes; Mango peel.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100009
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Compósito à base de poli(hidroxibutirato) (PHB) reforçado com fibras de rami espécie Bohemeria nivea. Repositório Alice
MARINHO, N. P.; BOLZON de MUÑIZ, G. I.; NISGOSKI, S.; KLOCK, U.; ANDRADE, A. S. de; MAGALHAES, W. L. E..
Fibras de rami da espécie Bohemeria nivea foram usadas como elemento de reforço, nas proporções de 5%, 10% e 15%, para melhorar as propriedades mecânicas nos esforços de tração do poli(hidroxibutirato). Para obtenção das fibras foi utilizado o processo de polpação organosolv com uma taxa de lignina residual (kappa) de k=46,2. Nas diferentes etapas de branqueamento das fibras foram obtidos k=29,2 (1a etapa com ClO2); k=24,3 (2a etapa com H2O2); k=19,0 (3a etapa com ClO2) e k=7,6 (4a etapa com H2O2). Nos ensaios mecânicos de tração o Módulo de Elasticidade (MOE) apresentou baixos Coeficientes de Variação (CV) entre 12,45% e 17,35% e pouca dispersão no Desvio Padrão estando entre 50,41MPa e 65,93MPa para amostras de PHB/rami-15% e PHB/rami-5%,...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Bohemeria nivea; Rami; Compósito; Organosolv; MOE; Módulo de elasticidade; PolihidróxiButirato; Composite; Polyhydroxybutyrate.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1028419
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Evaluation of short-chain-length polyhydroxyalkanoate accumulation in Bacillus aryabhattai BJM
Pillai,Aneesh Balakrishna; Kumar,Arjun Jaya; Thulasi,Kavitha; Kumarapillai,Harikrishnan.
Abstract This study was focused on the polyhydroxybutyrate (PHB) accumulation property of Bacillus aryabhattai isolated from environment. Twenty-four polyhydroxyalkanoate (PHA) producers were screened out from sixty-two environmental bacterial isolates based on Sudan Black B colony staining. Based on their PHA accumulation property, six promising isolates were further screened out. The most productive isolate PHB10 was identified as B. aryabhattai PHB10. The polymer production maxima were 3.264 g/L, 2.181 g/L, 1.47 g/L, 1.742 g/L and 1.786 g/L in glucose, fructose, maltose, starch and glycerol respectively. The bacterial culture reached its stationary and declining phases at 18 h and 21 h respectively and indicated growth-associated PHB production. Nuclear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus aryabhattai PHB10; Polyhydroxybutyrate; Domestic sewerage; Polymer characterization; 16S rRNA.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300451
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Identification of pesticide-degrading Pseudomonas strains as poly-B-hydroxybutyrate producers. Repositório Alice
CROCHEMORE, A. G.; MATTOS, M. L. T.; VENDRUSCOLO, C. T.; CASTRO, L. A. S. de; MOREIRA, A. da S..
2012
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pesticide-degrading; Polyhydroxybutyrate.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/941118
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New PHA products using unrelated carbon sources BJM
Matias,Fernanda; Rodrigues,Maria Filomena de Andrade.
Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type, Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources. The classification was based on 16S...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Burkholderia; Burkholderia cepacia complex; Genomovar-type; Mcl-PHA; Polyhydroxyalkanoates; Polyhydroxybutyrate.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400017
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Nutrient optimization for production of polyhydroxybutyrate from halotolerant photosynthetic bacteria cultivated under aerobic-dark condition Electron. J. Biotechnol.
Sangkharak,Kanokphorn; Prasertsan,Poonsuk.
Three halotolerant bacterial strains; Rhodobacter sphaeroides ES16 (the wild type) and the two mutant strains of R. sphaeroides ES16, namely N20 and U7, were cultivated in glutamate-malate (GM) medium and screened for production of polyhydroxybutyrate (PHB). The mutant strains N20 and U7 were found to accumulate PHB (53.9 and 42.0% of DCW, respectively) 3.6 and 2.8 times higher than the wild type strain (19.5% of DCW), respectively. R. sphaeroides N20 were selected for studies on the effects of nutrient and environmental conditions on PHB accumulation. The optimal condition was 4 g/l acetate, 0.02 g/l (NH4)2SO4, C/N ratio of 6:1, 1.0 g/l K2HPO4, 1.0 g/l KH2PO4 and 3% NaCl with initial pH at 7.0. Under this optimal condition, the maximum PHB accumulation...
Tipo: Journal article Palavras-chave: Aerobic-dark cultivation; Optimization; PHB; Polyhydroxybutyrate; Rhodobacter sphaeroides.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300009
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Polyhydroxybutyrate production using agro-industrial residue as substrate by Bacillus sphaericus NCIM 5149 BABT
Ramadas,Nisha V.; Singh,Sudheer Kumar; Soccol,Carlos Ricardo; Pandey,Ashok.
The aim of this work was to study the production of polyhydroxybutyrate (PHB) using agro- industrial residues as the carbon source. Seven substrates, viz., wheat bran, potato starch, sesame oil cake, groundnut oil cake, cassava powder, jackfruit seed powder and corn flour were hydrolyzed using commercial enzymes and the hydrolyzates assessed for selecting the best substrate for PHB production. Jackfruit seed powder gave the maximum production of PHB under submerged fermentation using Bacillus sphaericus (19%) at the initial pH of 7.5.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus sphaericus; Polyhydroxybutyrate; Jackfruit seed powder.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100003
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Production and characterization of poly-3-hydroxybutyrate from crude glycerol by Bacillus sphaericus NII 0838 and improving its thermal properties by blending with other polymers BABT
Sindhu,Raveendran; Ammu,Balakrishnan; Binod,Parameswaran; Deepthi,Sreelatha K.; Ramachandran,K. B.; Soccol,Carlos Ricardo; Pandey,Ashok.
The aim of this work was to study the production of poly-3-hydroxybutyrate (PHB) under nitrogen limited conditions by Bacillus sphaericus NII 0838 using crude glycerol from biodiesel industry as sole carbon source. Effect of various process parameters on PHB production such as glycerol concentration, inoculum size and pH of the medium were optimized. Characterization of extracted PHB was carried out by FT-IR, ¹H and 13C NMR. Results showed that the bacterial culture accumulated about 31% PHB in crude glycerol medium. The extracted PHB was blended with other polymers to improve its physical characteristics. The thermal properties of the polymer like melting temperature (Tm) and heat of fusion (ΔHf) were determined using DSC.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyhydroxybutyrate; Bacillus sphaericus; Polymer blends; Crude glycerol; Biodiesel industry.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400019
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Production of polyhydroxybutyrate (PHB) and biofilm by Azospirillum brasilense aiming at the development of liquid inoculants with high performance. Repositório Alice
SANTOS, M. S.; HUNGRIA, M.; NOGUEIRA, M. A..
bitstream/item/167300/1/2017SantosetalAfrJBiotechnol.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fixação de nitrogênio; Estimulante de crescimento vegetal; Polyhydroxybutyrate; Nitrogen fixation; Growth promotion.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080481
Registros recuperados: 9
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