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Biosynthesis of medium chain length poly-3-hydroxyalkanoates by Pseudomonas guezennei from various carbon sources ArchiMer
Simon-colin, Christelle; Raguenes, Gerard; Costa, Bernard; Guezennec, Jean.
The biosynthesis of poly-(3-hydroxyalkanoates) (PHAs) by Pseudomonas guezennei using glucose and/or fatty acids with chain-length from 3 to 18 carbon atoms as carbon sources was investigated. Glucose, acetate, pyruvate, propionate, valerate, hexanoate, heptanoate, octanoate, decanoate, and oleic acid were supplemented in the mineral medium as the sole carbon source or as a mixture for PHAs accumulation. Chemical composition of the resulting PHAs was analysed by coupled gas chromatography mass spectroscopy (GCMS), Fourier Transform Infrared Spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR). PHAs contained up to 10 different types of 3-hydroxyalkanoic acid units, including saturated 3-hydroxyacids from C4 to C14, and unsaturated monomers...
Tipo: Text Palavras-chave: Carbon substrates; Pseudomonas guezennei; Polymer characterization; Polyhydroxyalkanoates.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6115.pdf
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Evaluation of short-chain-length polyhydroxyalkanoate accumulation in Bacillus aryabhattai BJM
Pillai,Aneesh Balakrishna; Kumar,Arjun Jaya; Thulasi,Kavitha; Kumarapillai,Harikrishnan.
Abstract This study was focused on the polyhydroxybutyrate (PHB) accumulation property of Bacillus aryabhattai isolated from environment. Twenty-four polyhydroxyalkanoate (PHA) producers were screened out from sixty-two environmental bacterial isolates based on Sudan Black B colony staining. Based on their PHA accumulation property, six promising isolates were further screened out. The most productive isolate PHB10 was identified as B. aryabhattai PHB10. The polymer production maxima were 3.264 g/L, 2.181 g/L, 1.47 g/L, 1.742 g/L and 1.786 g/L in glucose, fructose, maltose, starch and glycerol respectively. The bacterial culture reached its stationary and declining phases at 18 h and 21 h respectively and indicated growth-associated PHB production. Nuclear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus aryabhattai PHB10; Polyhydroxybutyrate; Domestic sewerage; Polymer characterization; 16S rRNA.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300451
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