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WISBECK,ELISABETH; FACCHINI,JEAN M.; ALVES,ENDI P.; SILVEIRA,MARCIA L.L.; GERN,REGINA M.M.; NINOW,JORGE L.; FURLAN,SANDRA A.. |
ABSTRACT Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/L of glucose) and the pH (4 and 6) on kinetic parameters of production of biomass. The effectiveness of different doses (10, 30 and 50 mg/kg) of a mycelium polysaccharide fraction extracted from P. ostreatus in reducing Sarcoma 180 development in mice was also verified. In the range of this study, maximum concentration of mycelial biomass (about 12.8 g/L) was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antitumor Activity; Mycelium; Pleurotus ostreatus; Polysaccharides; Sarcoma 180. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502013 |
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MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R. |
The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to... |
Tipo: Text |
Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf |
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PEIXOTO, F. J. B.; SANTANA, H. M.; OLIVEIRA, L. A. de; JESUS, J. L. de; RANGEL, M. A. S.; SANTOS, V. da S.. |
A mandioca (Manihot esculenta Crantz) é uma das principais culturas responsáveis pela produção de alimento nos trópicos. O amido de mandioca pode ser usado no preparo de inúmeros produtos, como nas indústrias de alimentos, farmacêutica, de papel e celulose, química e têxtil. |
Tipo: Parte de livro |
Palavras-chave: Fator ácido; Mandioca; Amido; Amilose; PH; Cassava; Starch; Polysaccharides; Acidity. |
Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1011128 |
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YAN,Wensheng; JIANG,Lingjun; XU,Jifen. |
Abstract This study aimed to investigate the effects of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides (CPP) on the glucose-lipid metabolism in T2DM rats. T2DM model was established in 40 Wistar rats which were then randomly divided into model, CPP-1, CPP-2 and CPP-3 groups. Ten normal rats were selected as the control group. The CPP-1, CPP-2 and CPP-3 group were intragastrically administrated with 100, 200 and 400 mg/kg CPP for 4 weeks, respectively. Results showed that, during the treatment, compared with model group, in CPP treatment groups the T2DM symptoms were lightened, and the body weight was improved. After treatment, compared with model group, in CPP-3 group the blood fasting plasma glucose, fasting insulin and glycosylated hemoglobin... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cyclocarya paliurus (Batal.) Iljinskaja; Polysaccharides; Type 2 diabetes mellitus; Inflammatory response; Oxidative stress. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005028102 |
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COSTA, F. H. da S.; PEREIRA, M. A. A.; OLIVEIRA, J. P. de; PEREIRA, J. E. S.. |
Com este trabalho, objetivou-se avaliar a ação do ágar e sua substituição parcial e total pelo amido de mandioca na composição de meios de cultura de bananeira e abacaxizeiro. Gemas axilares de abacaxizeiro, cvs. Rio Branco e Quinari foram estabelecidas e avaliadas por quatro subcultivos quanto à multiplicação em meio de MS, suplementado com 2 mg.L-1 de BAP e 0,25 mg.L-1 de ANA, e os seguintes tratamentos: M1: ágar (5 g.L-1), M2: ágar (2,5 g.L-1) + fécula de mandioca (60 g.L-1), M3: fécula de mandioca (60 g.L-1), e M4: ágar (2,5 g.L-1) + fécula de mandioca (30 g.L-1). Num segundo experimento, por três subcultivos sucessivos brotações de bananeira da cv. Grand Naine foram avaliadas quanto à multiplicação em meio de cultura composto por: MM1: ágar (6 g.L-1)... |
Tipo: Artigo de periódico |
Palavras-chave: Agente gelificante; Bananos; Agentes de gelificación; Almidón de mandioca; Cultivo in vitro; Micropropagación; Piñas; Polisacáridas.; Banana; Musa sp; Abacaxi; Ananas comosus; Micropropagação; Cultura in vitro; Polissacarídeo; Amido; Agar.; Pineapples; Micropropagation; In vitro culture; Polysaccharides; Gelling agents; Cassava starch.. |
Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/498371 |
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Verçosa Júnior,D.; Ferraz,V.P.; Duarte,E.R.; Oliveira,N.J. F.; Soto-Blanco,B.; Cassali,G.D.; Melo,M.M.. |
The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Ehrlich solid tumor; Polysaccharides; Hematological profile. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300679 |
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FREITAS, M. B.; MEDUGNO, C. C.; SCHONS, R. F.; STADNIK, M. J.. |
O presente trabalho objetivou avaliar a eficácia da ulvana, um polissacarídeo proveniente de alga, em induzir resistência em Phaseolus vulgaris contra Colletotrichum lindemuthianum, quando formulada sozinha ou com caulinita, sílica amorfa ou atapulgita e armazenada por 0, 4, 8 ou 12 meses. Após cada período de armazenamento, as formulações e água (controle) foram aplicadas à folhagem de plantas de feijão (cv. Uirapuru, estádio fenológico V4) aos 6 e 3 dias antes da inoculação com C. lindemuthianum. A severidade da antracnose foi estimada em intervalos de 2 dias, com o aparecimento dos primeiros sintomas até 13 dias após a inoculação. A partir dos dados de severidade calculou-se a Área Abaixo da Curva de Progresso da Doença (AACPD). A aplicação de ulvana... |
Tipo: Artigo de periódico |
Palavras-chave: Atapulgita; Caulinita; Sílica amorfa; Ulvana.; Feijão; Colletotrichum lindemuthianum; Antracnose; Polissacarídeo; Controle biológico; Anthracnose; Biological control; Polysaccharides; Beans. |
Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/898329 |
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Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M.. |
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602 |
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Lebellenger, Lou. |
The marine bacteria Vibrio diabolicus produces an exopolysaccharide (EPS) called HE800. The native form of HE800 has already shown biological efficiency in bone healing. It is studied as glycosaminoglycan-mimetic for applications in cosmetic and human health. To enhance biotechnological applications of polysaccharides, the chemical structures should be properly controlled; moreover, the production yield could also be a bottleneck. The genetic cluster of the HE800 EPS biosynthesis has been identified in 2014. However, the biosynthesis molecular mechanisms and regulations remain sometimes unclear. The main objective of this work is to obtain a recombinant production of the HE800 EPS in the common host Escherichia coli, to improve the knowledge of its... |
Tipo: Text |
Palavras-chave: Vibrio diabolicus; Polysaccharides; HE800; GAGmimétiques; Biosynthèse; Vibrio diabolicus; Polysaccharides; HE800; GAGmimetics; Biosynthesis.. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00497/60911/64261.pdf |
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CAVALCANTE, M. M. da S. |
A manga (Mangifera indica L.) faz parte da família dos Anacardiaceae. O Brasil é um país de grande atividade agroindustrial e, com isso, produz uma quantidade considerável de resíduos que podem ser aproveitados para a obtenção de compostos fenólicos, mangiferina e pectina, que possuem aplicabilidade nas indústrias farmacêuticas, cosméticos, químicas e alimentícias. Dessa forma, o objetivo do presente trabalho foi avaliar o processo de extração de polifenóis, mangiferina e pectina da casca de manga. As extrações foram realizadas utilizando as técnicas de extração por solvente pressurizado (ESP) e a extração por soxhlet (técnica convencional). As condições de processo para o Soxhlet foram: 600 mL de metanol P.A na proporção de 1:60 (m/v) e no tempo de 9 h,... |
Tipo: Teses |
Palavras-chave: Tommy Atkins; ESP; Polysaccharides. |
Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105093 |
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Registros recuperados: 45 | |
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