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Registros recuperados: 33
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A method for germinating perennial Cicer species Open Agri
Coyne, C.J.
Palavras-chave: Polysaccharides; Chickpeas; Grafting; Pigeonpeas; Germplasm; Relative humidity; Transplanting; Regeneration; Tillage equipment; Developmental stages.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3229
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A polysaccharide fraction extracted from Pleurotus ostreatus mycelial biomass inhibit Sarcoma 180 tumor Anais da ABC (AABC)
WISBECK,ELISABETH; FACCHINI,JEAN M.; ALVES,ENDI P.; SILVEIRA,MARCIA L.L.; GERN,REGINA M.M.; NINOW,JORGE L.; FURLAN,SANDRA A..
ABSTRACT Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in submerged culture, evaluating the influence of the initial concentration of substrate (20 and 40 g/L of glucose) and the pH (4 and 6) on kinetic parameters of production of biomass. The effectiveness of different doses (10, 30 and 50 mg/kg) of a mycelium polysaccharide fraction extracted from P. ostreatus in reducing Sarcoma 180 development in mice was also verified. In the range of this study, maximum concentration of mycelial biomass (about 12.8 g/L) was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antitumor Activity; Mycelium; Pleurotus ostreatus; Polysaccharides; Sarcoma 180.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502013
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A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses Biol. Res.
MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
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Anatomical and histochemical aspects of zigotic embryo and leaves in 'Coqueiro Anão' Ciência Rural
Santana,Marlucia Cruz de; Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Teixeira,Sílvio Lopes.
The paper provides information about histochemical staining reactions in leaves and embryos of 'Coqueiro Anão' (Cocos nucifera). It was compared in vitro coconut and autotrophic palm leaves. Reactions for insoluble polysaccharides and acidic compounds, protein, extractable lipids, lignin and other classes of compounds were tested using histochemical tests. None sample gave positive reaction for lignin and phenolic compounds. All the samples gave positive reaction for protein, starch and insoluble polysaccharides while acidic compounds were positive only in in vitro leaves. Both in vitro and autotrophic leaves gave positive reaction for lipids showing presence of cuticle even in in vitro leaves. Only autotrophic palm leaves showed idioblasts containing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucifera; In vitro culture; Polysaccharides; Protein; Lipids.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400019
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Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins: study of the binding properties of fucoidan to antithrombin ArchiMer
Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R.
The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to...
Tipo: Text Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf
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Chemical composition of wild edible mushrooms and antioxidant properties of their water soluble polysaccharidic and ethanolic fractions IPB - Escola Superior Agrária
Vaz, Josiana A.; Barros, Lillian; Martins, Anabela; Santos-Buelga, Celestino; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R..
Mushrooms have become attractive as functional foods and as a source of physiologically beneficial bioactive compounds. Herein, we describe and compare the chemical constituents (phenolic compounds, macronutrients, sugars, fatty acids, tocopherols and ascorbic acid) of four wild edible mushrooms widely appreciated in gastronomy: Armillaria mellea (Vahl) P. Kumm, Calocybe gambosa (Fr.) Donk, Clitocybe odora (Fr.) P. Kumm., Coprinus comatus (O.F. Müll.) Pers. Furthermore, the antioxidant activity of their water soluble polysaccharidic and ethanolic fractions was studied by three different in vitro assays. Coprinus comatus revealed the highest concentrations of sugars (43.23 g/100 g dry weight), PUFA (77.46%), phenolic compounds (45.02 mg/Kg), tocopherols...
Tipo: Article Palavras-chave: Wild edible mushrooms; Bioactive compounds; Phenolic compounds; Polysaccharides; Chemical composition.
Ano: 2011 URL: http://hdl.handle.net/10198/3881
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Colleters in Chamaecrista (L.) Moench sect. Chamaecrista and sect. Caliciopsis (Leguminosae-Caesalpinioideae): anatomy and taxonomic implications Acta Botanica
Silva,Marinalva dos Santos; Coutinho,Ítalo Antônio Cotta; Araújo,Maicon Nascimento; Meira,Renata Maria Strozi Alves.
ABSTRACT The genus Chamaecrista contains ca. 330 species organized into six sections, of which section Chamaecrista is the second largest (ca.75 species) distributed among six series, while the section Caliciopsis contains only two species. Colleters have been described in the genus Chamaecrista and they show potential taxonomic and phylogenetic significance. They are associated with lubrication, desiccation prevention and protection from microbial attacks of young developing organs. Although six types of colleters have been described for the genus Chamaecrista, there have been no studies focusing on the diversity of colleters in the sections Chamaecrista and Caliciopsis. Samples from developing leaves and flowers of both sections were obtained from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colleter; Flower; Histochemical analysis; Leaf; Polysaccharides; Secretory structures; Taxonomy.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300382
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Comparative anatomy of calyx and foliar glands of Banisteriopsis C. B. Rob. (Malpighiaceae) Acta Botanica
Araújo,Josiane Silva; Meira,Renata Maria Strozi Alves.
Banisteriopsis is considered one of the largest genera of Malpighiaceae with 58 species, of which 47 occur in Brazil. The typical calyx and leaf glands of Banisteriopsis are considered relevant to the adaptive success of Malpighiaceae. Comparative studies of anatomical and histochemical characteristics may reveal similarities and assist in the interpretation of the functions performed by such glands. The present study aimed to describe the anatomy of the calyx and leaf glands of 38 species ofBanisteriopsis that occur in Brazil, and to analyze these structures histochemically in B. campestris, B. laevifolia and B malifolia, using standard methods. Calyx glands differ from leaf glands by possessing an irregular surface that is covered by a thick cuticle that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banisteriopsis; Calyx glands; Histochemistry; Leaf glands; Lipids; Micromorphology; Polysaccharides; Protein.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100112
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Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides alleviate type 2 diabetes mellitus in rats by resisting inflammatory response and oxidative stress Ciênc. Tecnol. Aliment.
YAN,Wensheng; JIANG,Lingjun; XU,Jifen.
Abstract This study aimed to investigate the effects of Cyclocarya paliurus (Batal.) Iljinskaja polysaccharides (CPP) on the glucose-lipid metabolism in T2DM rats. T2DM model was established in 40 Wistar rats which were then randomly divided into model, CPP-1, CPP-2 and CPP-3 groups. Ten normal rats were selected as the control group. The CPP-1, CPP-2 and CPP-3 group were intragastrically administrated with 100, 200 and 400 mg/kg CPP for 4 weeks, respectively. Results showed that, during the treatment, compared with model group, in CPP treatment groups the T2DM symptoms were lightened, and the body weight was improved. After treatment, compared with model group, in CPP-3 group the blood fasting plasma glucose, fasting insulin and glycosylated hemoglobin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclocarya paliurus (Batal.) Iljinskaja; Polysaccharides; Type 2 diabetes mellitus; Inflammatory response; Oxidative stress.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005028102
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Effects of different extracts of the mushroom Agaricus blazei Murill on the hematologic profile of mice with Ehrlich tumor Arq. Bras. Med. Vet. Zootec.
Verçosa Júnior,D.; Ferraz,V.P.; Duarte,E.R.; Oliveira,N.J. F.; Soto-Blanco,B.; Cassali,G.D.; Melo,M.M..
The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mouse; Ehrlich solid tumor; Polysaccharides; Hematological profile.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300679
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Enhancement of cellular activity in hyperglycemic mice dermal wounds dressed with chitosan-alginate membranes BJMBR
Breder,J.S.C.; Pires,A.L.R.; Azevedo,F.F.; Apolinário,P.P.; Cantaruti,T.; Jiwani,S.I.; Moraes,Â.M.; Consonni,S.R.; Araújo,E.P.; Adams,G.G.; Saad,M.J.A.; Lima,M.H.M..
The use of specially designed wound dressings could be an important alternative to facilitate the healing process of wounds in the hyperglycemic state. Biocompatible dressings combining chitosan and alginate can speed up wound healing by modulating the inflammatory phase, stimulating fibroblast proliferation, and aiding in remodeling phases. However, this biomaterial has not yet been explored in chronic and acute lesions of diabetic patients. The aim of this study was to evaluate the effect of topical treatment with a chitosan-alginate membrane on acute skin wounds of hyperglycemic mice. Diabetes mellitus was induced by streptozotocin (60 mg · kg-1 · day-1 for 5 days, intraperitoneally) and the cutaneous wound was performed by removing the epidermis using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C57BL/6J mice; Wound healing; Wound dressing; Polysaccharides; Diabetes.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000100602
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Etude de la biosynthèse des polysaccharides bactériens : vers la production recombinante d'hétéropolysaccharide ArchiMer
Lebellenger, Lou.
The marine bacteria Vibrio diabolicus produces an exopolysaccharide (EPS) called HE800. The native form of HE800 has already shown biological efficiency in bone healing. It is studied as glycosaminoglycan-mimetic for applications in cosmetic and human health. To enhance biotechnological applications of polysaccharides, the chemical structures should be properly controlled; moreover, the production yield could also be a bottleneck. The genetic cluster of the HE800 EPS biosynthesis has been identified in 2014. However, the biosynthesis molecular mechanisms and regulations remain sometimes unclear. The main objective of this work is to obtain a recombinant production of the HE800 EPS in the common host Escherichia coli, to improve the knowledge of its...
Tipo: Text Palavras-chave: Vibrio diabolicus; Polysaccharides; HE800; GAGmimétiques; Biosynthèse; Vibrio diabolicus; Polysaccharides; HE800; GAGmimetics; Biosynthesis..
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00497/60911/64261.pdf
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Etude de la réponse immunitaire chez l'huître Crassostrea gigas : caractérisation et fonctions de protéines de reconnaissance aux LPS et d'effecteurs antimicrobiens ArchiMer
Gonzalez, Marcelo.
Research on the immune response of oysters is motivated by the problems of infectious diseases potentially found in the farms. Over the course of the last few years, the development of genomic approaches has allowed us to make headway on characterising genes involved in the cup oyster's (Crassostrea gigas) innate immunity, particularly thanks to various programmes for analysing transcriptome, of type EST (Express Sequence Tag) and subtractive banks. Within the context of this work, over the course of which we have used the data from genomic research and we have also developed biochemical approaches, we were particularly interested in studying the proteins involved in reconnaissance reactions to LPS as well as to antimicrobial effectors. In an earlier part,...
Tipo: Text Palavras-chave: Defense peptides; Polysaccharides; LPS; Hemocytes; Immune response; Crassostrea gigas; Shellfish culture; LPS; Défensine; Peptides antimicrobiens; LBP; BPI; Superoxide dismutase; Hémocytes; Crassostrea gigas; Huître; Mollusque; Immunité.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2098.pdf
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Evaluation of sorghum germplasm for resistance to insect pests Open Agri
H.C..
Palavras-chave: Biological interaction; Sorghum; Genotypes; Germplasm; Animal developmental stages; Grain; Diapause; Sowing date; Eggs; Polysaccharides.
Ano: 2003 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4232
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Extraction of Soluble Polysaccharides from Grape Skins Ciencia e Investigación Agraria
Gil Cortiella,Mariona; Peña-Neira,Álvaro.
Obtaining polysaccharides from grape cell walls is an interesting topic for the wine industry given its implications with regards to wine treatments and potential sensory attributes. In the present work, several conditions (i.e., a chelating agent, pH, titratable acidity, extraction time, number of successive extractions, solid to liquid ratio and alcoholic fermentation) were tested to assess their effects on the yield of soluble polysaccharides obtained from grape skins. Among the four tested solvents (i.e., water, tartaric acid, ammonium oxalate, and ethylenediaminetetraacetic acid), aqueous ammonium oxalate was the most effective for polysaccharide extraction but also produced the highest amount of protein impurities. Increasing the number of successive...
Tipo: Journal article Palavras-chave: Carménère; Extraction; Grape skin polysaccharides; Pectin; Polysaccharides; Wine.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000100009
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Glycoconjugates and polysaccharides of fungal cell wall and activation of immune system BJM
Pinto,M.R.; Barreto-Bergter,E.; Taborda,C.P..
The fate of organochlorine 14C-dicofol in activated sludge process was investigated. Results showed that the major part of radioactivity remained adsorbed on biological sludge. Consequently, its final disposal deserves special attention. The small amounts of dicofol, biotransformed or not, which remained in the treated effluent could contaminate receiving bodies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycoproteins; Polysaccharides; Glycosphingolipids; Immune system; Fungi.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200001
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Incidence of hydrocolloid type on quality parameters in mango leathers (Mangifera indica L.) Yulima variety Ciênc. Tecnol. Aliment.
SÁNCHEZ RIAÑO,Andrea Milena; BERMEO ANDRADE,Helga Patricia; VALENZUELA REAL,Claudia Patricia.
Abstract The effect of four hydrocolloids on the physico-chemical, bioactive and rheological properties in sweetened mango leather sheets of the Yulima variety were analyzed. Parametric and non-parametric statistical tests were done to analyze the differences among samples (four of treatment against two of control). The findings show significant positive effects caused by the hydrocolloids made from Gum Arabic (AG), Maltodextrin (MTD) and Citric Slow Pectin (CSP) on leathers’ quality and appearance attributes. Only the Carboxymethylcellulose (CMC) reported adverse effects on the mango leather's quality; therefore, its use on an industrial scale is not recommended for this line of processed product.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fruit leathering; Mango pulp; Food additives; Polysaccharides; Quality attributes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000500109
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Isolation of high-quality RNA from Platycladus orientalis and other Cupressaceae plants Electron. J. Biotechnol.
Chang,Ermei; Zhao,Yuxin; Wei,Qianchun; Shi,Shengqing; Jiang,Zeping.
Platycladus orientalis has a lifespan of several thousand years in China, making it a good plant in which to study aging at the molecular level, but this requires sufficient quantities of high-quality P. orientalis RNA. However, no appropriate methods have been reported for total RNA isolation from P. orientalis leaves. The TRIzol method did not extract RNA, while cetyltrimethylammonium bromide, sodium dodecyl sulfate-phenol, and plant RNAout kit (Tianz, Inc., China) protocols resulted in low yields of poor quality RNA. Isolating total RNA using the Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) resulted in a high-quality product but a low yield. However, the two-step removal of polyphenols and polysaccharides in the improved plant...
Tipo: Journal article Palavras-chave: Cupressaceae plants; Polysaccharides; Polyphenols; Total RNA isolation; Two-step removal of polyphenols and polysaccharides.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500004
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Isolation of total RNA from tissues rich in polyphenols and polysaccharides of mangrove plants Electron. J. Biotechnol.
Rubio-Piña,Jorge A; Zapata-Pérez,Omar.
RNA extraction from mangrove tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds upon cell disruption. Besides, a successful RNA isolation from mangrove tissues sometimes can be strain and species specific. Two different methods were used to extract RNA from tissues (stems, leaves and roots) of black mangrove (Avicennia germinans) and white mangrove (Laguncularia racemosa), collected from the mangrove area at Progreso, Yucatan, Mexico. An optimized and modified total RNA isolation method was developed for these recalcitrant species. The protocol is based on the CTAB method including β-mercaptoethanol and PVPP with sequential Phenol/Chloroform/isoamyl alcohol extractions to remove protein, and polyphenols,...
Tipo: Journal article Palavras-chave: Mangrove; Plant tissues; Polysaccharides; RNA isolation.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500011
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Mechanical and water vapor permeability properties of biodegradables films based on methylcellulose, glucomannan, pectin and gelatin Ciênc. Tecnol. Aliment.
Chambi,Hulda Noemi Mamani; Grosso,Carlos Raimundo Ferreira.
Mimic biological structures such as the cell wall of plant tissues may be an alternative to obtain biodegradable films with improved mechanical and water vapor barrier properties. This study aims to evaluate the mechanical properties and water vapor permeability (WVP) of films produced by using the solvent-casting technique from blended methylcellulose, glucomannan, pectin and gelatin. First, films from polysaccharides at pH 4 were produced. The film with the best mechanical performance (tensile strength = 72.63 MPa; elongation = 9.85%) was obtained from methylcellulose-glucomannan-pectin at ratio 1:4:1, respectively. Then, gelatin was added to this polysaccharide blend and the pH was adjusted to 4, 5 and 6. Results showed significant improvement in WVP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polysaccharides; Proteins; Films; Biodegradable materials.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000300029
Registros recuperados: 33
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