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Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani 69
Shu,Canwei; Sun,Si; Chen,Jieling; Chen,Jianyi; Zhou,Erxun.
Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and contain a large quantity of polysaccharides, lipids, proteins and pigments. In order to extract high-quality total RNA from the sclerotia of R. solani, five methods, including E.Z.N.A.™ Fungal RNA Kit, sodium dodecyl sulfate (SDS)-sodium borate, SDS-polyvinylpyrrolidone (PVP), guanidinium thiocyanate (GTC) and modified Trizol, were compared in this study. Results The electrophoresis results showed that it failed to extract total RNA from the...
Tipo: Journal article Palavras-chave: Polyvinylpyrrolidone; Rhizoctonia solani; RT-PCR; Sclerotia; Sodium dodecyl sulfate; Total RNA extraction.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100009
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Formulation and characterization of gastroretentive discs containing famotidine 52
Raval,Jignyasha Amitkumar; Patel,Madhabhai M.
In this work a different type of formulation, as disc, containing a selected mucoadhesive polymer, fillers, and binders were investigated for their potential as a mucoadhesive gastroretentive delivery system to deliver famotidine in the stomach. Various types of hydrophilic diluents were evaluated for their swelling and mucoadhesive property and one (polyvinylpyrrolidone, PVP) was selected to combine with the selected mucoadhesive polymer (polyethylene oxide, PEO). Discs with different ratios of PEO and PVP were prepared and evaluated for swelling, dissolution, and mucoadhesion. The swelling property of the discs increased as the concentration of PEO was increased and also did the mucoadhesion. These discs retained their integrity and adherence onto...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucoadhesion; Discs; Gastroretentive; Polyethylene oxide; Polyvinylpyrrolidone.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200010
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