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| SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F.. |
| Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase,... |
| Tipo: Artigo de periódico |
Palavras-chave: Enzima; Clonagem; Programa de Computador; Peptídeo; Metagenomics; Enzymes; Cloning (animals); Signal peptide; Prevotella; Computer software; Screening. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129624 |
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| PAZ VENTERO, M.; CUADRAT, R. R. C.; VIDAL, I.; ANDRADE, B. G. N.; MOLINA-PARDINES, C.; HARI-MORENO, J. M.; COUTINHO, F. H.; MERINO, E.; REGITANO, L. C. de A.; SILVEIRA, C. B.; AFLI, H.; LÓPEZ-PÉREZ, M.; RODRÍGUEZ, J. C.. |
| Background: SARS-CoV-2 is an RNA virus causing COVID-19. The clinical characteristics and epidemiology of COVID-19 have been extensively investigated, however, only one study so far focused on the patient?s nasopharynx microbiota. In this study we investigated the nasopharynx microbial community of patients that developed different severity levels of COVID-19. We performed 16S ribosomal DNA sequencing from nasopharyngeal swab samples obtained from SARS-CoV-2 positive (56) and negative (18) patients in the province of Alicante (Spain) in their first visit to the hospital. Positive SARS-CoV-2 patients were observed and later categorized in mild (symptomatic without hospitalization), moderate (hospitalization), and severe (admission to ICU). We compared the... |
| Tipo: Artigo de periódico |
Palavras-chave: COVID 19; SARS CoV 2; NGS next generation sequencing; Coronavirus; Microbiome; Prevotella. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132766 |
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