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Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica) BABT
Llamoca-Zárate,Romulo Marino; Ponte,Luiz Ferreira Aguiar; Landsmann,Joerg; Campos,Francisco de Assis Paiva.
We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica. DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 m m and the distance between the flying disk and the target tissue was 7.5cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Opuntia ficus-indica; Prickly-pear; Cactaceae; Transient gene expression; Particle bombardment.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300005
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Isolation and characterization of a reserve protein from the seeds of Opuntia ficus-indica (Cactaceae) BJMBR
Uchoa,A.F.; Souza,P.A.S.; Zarate,R.M.L.; Gomes-Filho,E.; Campos,F.A.P..
We describe here the isolation and characterization of a major albumin from the seeds of Opuntia ficus-indica (Cactaceae). This protein has a molecular mass of 6.5 kDa and was isolated by a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of this protein was determined and it was shown to have similarities with the amino acid composition of several proteins from the 2S albumin storage protein family. The N-terminal amino acid sequence of this protein is Asp-Pro-Tyr-Trp-Glu-Gln-Arg.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cactaceae; Prickly-pear; Opuntia ficus-indica; Seed proteins; 2S albumins.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600005
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