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Registros recuperados: 13
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AtchitIV gene expression is stimulated under abiotic stresses and is spatially and temporally regulated during embryo development Genet. Mol. Biol.
Gerhardt,Liliane B. de A.; Magioli,Cláudia; Perez,Ana B.U.C.M.; Margis,Rogério; Sachetto-Martins,Gilberto; Margis-Pinheiro,Márcia.
The expression of AtchitIV gene was analysed in Arabidopsis plants submitted to abiotic stresses. Transcript accumulation was detected in leaves in response to UV light exposure, exogenous salicylic acid administration and wounding. Transgenic Arabidopsis plants carrying AtchitIV promoter::gus fusion also showed differential expression of the reporter gene in response to these treatments. The AtchitIV expression was also analysed during Arabidopsis embryo development. GUS assay demonstrated AtchitIV promoter activation in zygotic embryos from torpedo stage up to full maturation. Promoter deletion analysis indicated that all the 5' cis-acting elements responsible for the specific tissue expression are located in a region of 1083 bp, adjacent to the start of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abiotic stress; Chia4 Arabidopsis thaliana endochitinase; Embryogenesis; Gene expression; Promoter.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100020
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Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): Comparison with salmonid species and analysis of CpG methylation ArchiMer
Geay, Florian; Zambonino, Jose-luis; Reinhardt, Richard; Kuhl, Heiner; Santigosa, Ester; Cahu, Chantal; Mazurais, David.
Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by quantitative PCR the relative amounts of FADS2 mRNA in European sea bass (Dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (Oncorhynchus mykiss L.). The analysis of the expression data was performed regarding the difference of the characteristics of a critical fragment of the fads2 gene promoter between sea bass and Atlantic salmon. The lower level of fads2 gene expression observed in sea bass suggested that fads2 gene putative promoter, which exhibited an...
Tipo: Text Palavras-chave: FADS2; Sea bass; Gene expression; Promoter; Methylation.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00065/17621/15310.pdf
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Cloning of high molecular weight gluten subunit promoter and study on its function in wheat BABT
Weibo,Jin; Jin,Liu; Fangli,Wu; Aiguang,Guo.
The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Glutenin; Promoter; Endosperm-specific; Transient expression.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002
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El perfil del promotor de créditos de FR en el cumplimiento de sus metas en el estado de Chihuahua. Colegio de Postgraduados
Tovar Rangel, Delfino.
La discusión de considerar a un empleado por su perfil proporcionado por su formación académica o considerar por sus capacidades laborales, habilidades, destreza y aptitudes para desarrollar sus funciones de una forma eficaz y eficiente para lograr tener éxito, en este caso, el promotor de financiamiento rural en el estado de Chihuahua para lograr cumplir con sus metas en Financiera Rural hipotéticamente, es necesario que sea agrónomo o de carrera afín. En el presente estudio, en la discusión, análisis, revisión y conclusión de los datos e información recolectada mediante encuestas telefónicas, revisión bibliográfica, consultas en Internet y en la red Institucional. Se emiten los comentarios concluyentes de que, dado que el promotor de crédito rural en...
Palavras-chave: Desarrollo rural; Crédito; Promotor; Perfil; Capacidades; Rural development; Credit; Promoter; Profile; Capabilities; Gestión Financiera para el Desarrollo Rural; Maestría Tecnológica.
Ano: 2014 URL: http://hdl.handle.net/10521/2392
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Genome walking in Sugarcane: an approach to clone unknown flanking sequences. Repositório Alice
LEAO, A. P.; SOUTO, B. de M.; DIAS, B. B. A.; MOLINARI, H. B. C..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sugarcane; Genome walking; Promoter; Genes; Cell wall.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908733
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Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato. Repositório Alice
QUINTERO, F. O. C.; PINTO, L. G.; BARSALOBRES-CAVALLARI, C. F.; ARCURI, M. de L. C.; PINO, L. E.; PERES, L. E. P.; MALUF, M. P.; MAIA, I. G..
In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tomato; Promoter; Seed maturation protein; Seed-specific expression; Coffee; Café.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113247
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Interleukin-6 promoter polymorphisms (-174 G/C) in Malaysian patients with systemic lupus erythematosus BJMBR
Chua,K.H.; Kee,B.P.; Tan,S.Y.; Lian,L.H..
Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that involves the inflammation of various organs upon deposition of immune complexes and is characterized by uncontrolled B cell hyperactivity. Despite intensive research on the etiology of the disease, the exact cause of the onset of SLE is unknown. The pathogenesis of the disease has been proposed to be associated with the imbalance of T helper type 1 (Th1) and Th2 cytokine activities. Elevated serum levels of interleukin-6 (IL-6), a Th2 cytokine with various functions in the regulation of human biological systems, are observed in SLE patients. In the present study, 100 Malaysian SLE patients and 100 controls were evaluated in order to determine the association of polymorphisms existing...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Interleukin-6; Systemic lupus erythematosus; Promoter; Polymorphisms.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600012
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Molecular cloning, expression pattern, and putative cis-acting elements of a 4-coumarate:CoA ligase gene in bamboo (Neosinocalamus affinis) Electron. J. Biotechnol.
Cao,Ying; Hu,Shang-lian; Huang,Sheng-xiong; Ren,Peng; Lu,Xue-qin.
Background: 4-coumarate:CoA ligase (4CL) plays an important role at the divergence point from general phenylpropanoid metabolism to several branch pathways. Although 4CL sin higher plants have been extensively studied, little has known about the 4CL gene of bamboo. Results: In current study, a Na4CL gene putative encoding 4-coumarate:CoA ligase (4CL) and its 5’-flanking region were isolated from bamboo (Neosinocalamus affinis) by RACE-PCR and genomic DNA walker, respectively. Na4CL encodes a predicted protein of 557 amino acids, with conserved motifs of adenylate-forming enzymes. Phylogenetic analysis showed that Na4CL shared 62~85% identity with other known plant 4CLs, and cluster closely with some known 4CLs in monocots. Sequence analysis...
Tipo: Journal article Palavras-chave: Expression pattern; Neosinocalamus affinis; Promoter; RACE; RNAi.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500009
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Potential regulatory elements on PCDH7 gene affecting residual feed intake in Nelore cattle. Repositório Alice
LIMA, A. O. de; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; GEISTLINGER, L.; ROCHA, M. I. P.; DINIZ, W. J. da S.; AFONSO, J.; BUSS, C. E.; PETRINI, J.; MOURAO, G. B.; COUTINHO, L. L.; REGITANO, L. C. de A..
Editores: Luiz Lehmann Coutinho, ESALQ/USP; Luciana Correia de Almeida Regitano, Embrapa Pecuária Sudeste; Gerson Barreto Mourão, ESALQ/USP; Aline Silva Mello Cesar, ESALQ/USP; Bárbara Silva Vignato, FZEA/USP; Mirele Daiana Poleti, ESALQ/USP; Wellison Jarles da Silva Diniz, UFSCar.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Residual feed intake; Promoter; Transcription factor; SNPs.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1075636
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Rules extraction from neural networks applied to the prediction and recognition of prokaryotic promoters Genet. Mol. Biol.
Silva,Scheila de Avila e; Gerhardt,Günther J.L.; Echeverrigaray,Sergio.
Promoters are DNA sequences located upstream of the gene region and play a central role in gene expression. Computational techniques show good accuracy in gene prediction but are less successful in predicting promoters, primarily because of the high number of false positives that reflect characteristics of the promoter sequences. Many machine learning methods have been used to address this issue. Neural Networks (NN) have been successfully used in this field because of their ability to recognize imprecise and incomplete patterns characteristic of promoter sequences. In this paper, NN was used to predict and recognize promoter sequences in two data sets: (i) one based on nucleotide sequence information and (ii) another based on stability sequence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neural network; Promoter; Rule extraction.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200031
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Simplified Transformation of Ostreococcus tauri Using Polyethylene Glycol ArchiMer
Sanchez, Frédéric; Geffroy, Solene; Norest, Manon; Yau, Sheree; Moreau, Hervé; Grimsley, Nigel.
Ostreococcus tauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13–22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic data produced from marine samples. Here we describe a simplified and robust transformation protocol for the smallest of these algae (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than the previously described electroporation protocol. Short (2 min or less) incubation times in PEG gave >104 transformants per microgram DNA. The time of cell recovery after transformation could be reduced to a few hours, permitting the experiment to be...
Tipo: Text Palavras-chave: Chlorophyta; Prasinophyte; Mamiellophyceae; Ostreococcus; Plankton; Picoeukaryote; Ecosystem; Bioluminescence; Promoter; Luciferase; Gene expression.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00500/61170/64639.pdf
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The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle Biol. Res.
ZIMMERMANN,KATRIN; HOLTZ,MARLIS; PHI-VAN,LOC.
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase
Tipo: Journal article Palavras-chave: Chromatin structure; Origin of bidirectional replication; Lysozyme; GAS41; Promoter.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009
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The promoter of a gene encoding an isoflavon reductase-like protein in coffee (Coffea arabica) drives a stress-responsive expression in leaves. Repositório Alice
BRANDALISE, M.; SEVERINO, F. E.; MALUF, M. P.; MAIA, I. G..
A cDNA clone (designated CaIRL) encoding an isoflavone reductase-like protein from coffee (Coffea arabica) was retrieved during a search for genes showing organ/tissue-specific expression among the expressed sequence tags (EST) of the Brazilian coffee EST database. The CaIRL cDNA contains a single open reading frame of 946 nucleotides (nt) encoding 314 amino acids (predicted molecular weight of 34 kDa). Several features identified the predicted CaIRL protein as a new member of the PIP family of NADPH-dependent reductases. Expression studies demonstrated that CaIRL is expressed exclusively in coffee leaves and its transcript level is markedly increased in response to fungal infection and mechanical injury. Analysis of transgenic tobacco plants harboring a...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coffee; Promoter; Tissue-specificity; Gene expression; Isoflavone-like proteins.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/880372
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