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Registros recuperados: 48 | |
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ORSI,RICARDO O.; SANTOS,VIVIAN G. DOS; PEZZATO,LUIZ E.; CARVALHO,PEDRO L.P.F. DE; TEIXEIRA,CAROLINE P.; FREITAS,JAKELINE M.A.; PADOVANI,CARLOS R.; SARTORI,MARIA M.P.; BARROS,MARGARIDA M.. |
ABSTRACT The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 Nile tilapia (50.0 ± 5.7 g fish-1). Hemato-immune parameters were analyzed before and after the bacterial challenge. Red blood cell, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were evaluated. The MIC of the EEP was 13% (v/v) with a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Health; Hematology; Immunology; Oreochromis niloticus; Propolis; Stress. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401785 |
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Sawaya,Alexandra C. H. F.; Souza,Kirllian S.; Marcucci,Maria C.; Cunha,Ildenize B.S.; Shimizu,Mario T.. |
Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propolis; Thin layer chromatography; Bioauthography; High performance liquid chromatography; Extraction methods; Minimal bactericidal concentration. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100017 |
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Muli,E. M.; Maingi,J. M.. |
The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted using four different concentrations of ethanol: pure, 70%, 50%, and 30%. Ethanol (70%) and Streptomycin were used as controls. The agar diffusion method using filter paper disks was employed. Antibacterial activity was determined as an equivalent of the inhibition zones diameters (in millimeters) after incubation at 37°C for 24h. Significant differences in the antibacterial activities of propolis were observed among the three regions, depending on the test... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apis mellifera; Propolis; Antibacterial agents; Disk diffusion antimicrobial tests. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300008 |
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Ferreira,Daiane; Unfer,Taís Cristina; Rocha,Hélio Carlos; Kreutz,Luiz Carlos; Koakoski,Gessi; Barcellos,Leonardo José Gil. |
An experiment was conducted to evaluate the potential of honey, propolis, and bee pollen for the reversal of lipid peroxidation induced by tebuconazole (TEB) in South American catfish (Rhamdia quelen), in which the concentration of thiobarbituric acid reactive substances (TBARS), the activity of the antioxidant enzyme glutathione-S-transferase (GST) and the concentrations of non-enzymatic antioxidants, reduced glutathione (GSH), ascorbic acid, and non-protein thiols were assessed. Honey (0.125 g L-1) and bee pollen (0.05 g L-1) added to the water reverse the production of TBARS induced by TEB, while propolis demonstrated a pro-oxidant effect, inducing an increase in TBARS production. The data presented herein suggest that the addition of water to honey and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Bee pollen; Honey; Lipid peroxidation; Propolis; Tebuconazole. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100021 |
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Borges,Kleiton Silva; Brassesco,María Sol; Scrideli,Carlos Alberto; Soares,Ademilson Espencer Egea; Tone,Luiz Gonzaga. |
Propolis is a resin formed by a complex chemical composition of substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine, due to its biological properties, that include antimicrobial, anti-inflammatory, antitumoral and immunomodulatory activities. Glioblastoma is the most common human brain tumor. Despite the improvements in GBM standard treatment, patients' prognosis is still very poor. The aim of this work was to evaluate in vitro the Tubi-bee propolis effects on human glioblastoma (U251 and U343) and fibroblast (MRC-5) cell lines. Proliferation, clonogenic capacity and apoptosis were analyzed after treatment with 1 mg/mL and 2 mg/mL propolis concentrations for different time periods. Additionally,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glioblastoma; Propolis; Temozolomide; U251; U343. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200024 |
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Sá,Rafael A. de; Castro,Frederico A.V. de; Eleutherio,Elis C.A.; Souza,Raquel M. de; Silva,Joaquim F.M. da; Pereira,Marcos D.. |
Propolis is a natural product widely used for humans. Due to its complex composition, a number of applications (antimicrobial, antiinflammatory, anesthetic, cytostatic and antioxidant) have been attributed to this substance. Using Saccharomyces cerevisiae as a eukaryotic model we investigated the mechanisms underlying the antioxidant effect of propolis from Guarapari against oxidative stress. Submitting a wild type (BY4741) and antioxidant deficient strains (ctt1∆, sod1∆, gsh1∆, gtt1∆ and gtt2∆) either to 15 mM menadione or to 2 mM hydrogen peroxide during 60 min, we observed that all strains, except the mutant sod1∆, acquired tolerance when previously treated with 25 µg/mL of alcoholic propolis extract. Such a treatment reduced the levels of ROS... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propolis; Antioxidant; Oxidative stress; Saccharomyces cerevisiae. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300051 |
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Azevedo,Rosa Vitória Palamin; Komesu,Marilena Chinalli; Candido,Regina Celia; Salvetti,Cristiane; Rezende,Fausto Hanaoka Caetano. |
Fifty individuals of both sexes aged on average 45.2 years were evaluated at the Semiology Clinic of FORP-USP in order to isolate and identify yeasts from the oral cavity, with and without lesions, and to determine the maximal inhibitory dilution (MID) of the commercial products Propolis (Apis-Flora) and Periogard (Colgate) against the strains isolated. Yeasts of the genus Candida were detected in the saliva of 9/19 (47.4%) individuals with a clinically healthy mouth, 18/22 (81.8%) of individuals with oral lesions, and in 4/9 (44.4%) of patients with deviation from normality, and were detected in 19/22 (86.4%) of the lesions. In the group with oral candidiasis, we isolated in tongue and lesion, respectively for each specie: C.tropicalis (8% and 10.7%),... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oral candidiasis; Propolis; Chlorhexidine; Yeasts. |
Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400008 |
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Barrientos,Leticia; Herrera,Christian L.; Montenegro,Gloria; Ortega,Ximena; Veloz,Jorge; Alvear,Marysol; Cuevas,Alejandro; Saavedra,Nicolás; Salazar,Luis A.. |
Propolis is a non-toxic natural substance with multiple pharmacological properties including anticancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propolis; Botanical characterization; Chemical characterization; Streptococcus mutans; Streptococcus sobrinus. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200038 |
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Torres,A.R.; Sandjo,L.P.; Friedemann,M.T.; Tomazzoli,M.M.; Maraschin,M.; Mello,C.F.; Santos,A.R.S.. |
In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stingless bees; Propolis; Antimicrobial activity; S. aureus; M. quadrifasciata quadrifasciata; T. angustula. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617 |
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Pereira-Filho,Rose Nely; Batista,Fellipe Santos; Ribeiro,Danielle Rodrigues; Melo,Genecy Calado de; Reis,Francisco Prado; Melo,Allan Ulisses Carvalho de; Gomes,Margarete Zanardo; Cardoso,Juliana Cordeiro; Albuquerque Júnior,Ricardo Luiz Cavalcanti de. |
The aim of this study was to assess the effect of oral administration of Hydroalcoholic Extract of Green Propolis (HEGP) on dermal carcinogenesis in rodent model. For the biological assay, we used 36 mice, assigned into 6 groups (n=6): CTR (treated with 100 mg/kg HEGP and no tumor induction), TUM (treated with water and tumor induction), GP10 (treated with 10 mg/kg HEGP and tumor induction), GP50 (treated with 50 mg/kg HEGP and tumor induction) and GP100 (treated with 100 mg/kg HEGP and tumor induction). Cancer induction was performed in the back of the mice by topical application of DMBA. After 16 weeks, mice were euthanized and their backs were submitted to post-mortem histological analysis. The mean number of lesions developed in TUM (4.14±0.89) was... |
Tipo: Journal article |
Palavras-chave: Propolis; Skin cancer. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200024 |
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Moraes,Cleber Silveira; Daugsch,Andreas; Li,Hongzhen; Rhim,Johng Suk; Park,Yong Kun. |
Two groups of propolis, group 12, which was collected in the southeastern Brazil and group 13, which was collected in the northeastern Brazil, were examined for antiproliferation of primary malignant tumor (RC-58T/h/SA#4)-derived human prostate cancer cells and human prostate epithelial cells. The strongest inhibition of RC-58T/h/SA#4 cells was observed in propolis group 13 extracts, whereas moderate growth inhibition was observed in human prostate epithelial cells in comparison with group 12. It can be said that the Brazilian propolis of group 13 contains important chemical ingredients. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prostate cancer; Antiproliferation; Propolis. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200038 |
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Albuquerque-Júnior,Ricardo Luiz Cavalcanti de; Barreto,André Luiz Santos; Pires,Jônatas Almeida; Reis,Francisco Prado; Lima,Sônia Oliveira; Ribeiro,Maria Amália Gonzaga; Cardoso,Juliana Cordeiro. |
Propolis, an apitherapic product widely employed in natural medicine, presents various biological properties, such as bactericidal and anti-inflammatory activities, but only few reports have looked at the Brazilian red propolis variety. Collagen films have been employed to improve wound healing and some studies have investigated the effect of natural products incorporated into these films. Thus, the aim of this study was to analyze the effect of collagen-based films containing red propolis on wound healing. Therefore, wounds performed in the back of rats were dressed with collagen-based films (G2), and collagen-based films containing red propolis (G3); undressed worked as control (G1). After 3, 7 and 14 days the animals were euthanized, and the wounds were... |
Tipo: Journal article |
Palavras-chave: Propolis; Wound healing; Occlusive dressings; Type-I collagen. |
Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400025 |
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Saavedra,Nicolás; Barrientos,Leticia; Herrera,Christian L; Alvear,Marysol; Montenegro,Gloria; Salazar,Luis A. |
Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of new antibacterial products after a greater resistance to those already known. Propolis has been used since ancient times, so their effects against various microorganisms have been already investigated. In our study, we evaluated the antimicrobial effect of 6 commercial ethanolic propolis extracts on the bacterium Lactobacillus fermentum. This bacterium was isolated after its identification by Polymerase Chain Reaction using species specific primers, and after... |
Tipo: Journal article |
Palavras-chave: Lactobacillus fermentum; Dental caries; Propolis; Antibacterial activity. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011 |
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Orsi,R. O.; Sforcin,J. M.; Funari,S. R. C.; Gomes,J. C.. |
The direct effect of ethanolic extract of propolis on guinea pig lung cell suspension containing mast cells, as well as its influence on the histamine release induced by antigen (ovoalbumin 10 mug/ml) and ionophore A 23187 (3 muM) were investigated. Propolis ethanolic extract (300 mug/ml) increased the histamine release in guinea pig lung suspension containing mast cells by a cytotoxic effect. Lower concentrations of propolis had no effect on histamine release. Our results demonstrated that propolis (3, 10, 30, and 100 mug/ml) shows no significant effect on the histamine release induced by ionophore and antigen. Based on these results, we suggest that propolis could directly activate mast cells, promoting inflammatory mediators release by cytotoxic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mast cells; Allergy; Histamine; Propolis. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100009 |
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Registros recuperados: 48 | |
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