|
|
|
| Registros recuperados: 27 | |
|
|
| Souza,Paula Monteiro de; Bittencourt,Mona Lisa de Assis; Caprara,Carolina Canielles; Freitas,Marcela de; Almeida,Renata Paula Coppini de; Silveira,Dâmaris; Fonseca,Yris Maria; Ferreira Filho,Edivaldo Ximenes; Pessoa Junior,Adalberto; Magalhães,Pérola Oliveira. |
| Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have dominated applications in industrial sectors. Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce production. Proteases can be produced in large quantities in a short time by established methods of fermentation. The parameters such as variation in C/N ratio, presence of some sugars, besides several other physical factors are important in the development of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteases; Enzyme production; Fungal protease; Industrial application. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200337 |
| |
|
|
| Cunha,Rodrigo L. O. R.; Gouvea,Iuri E.; Juliano,Luiz. |
| Tellurium is a rare element which has been regarded as a toxic, non-essential trace element and its biological role is not clearly established to date. Besides of that, the biological effects of elemental tellurium and some of its inorganic and organic derivatives have been studied, leading to a set of interesting and promising applications. As an example, it can be highlighted the uses of alkali-metal tellurites and tellurates in microbiology, the antioxidant effects of organotellurides and diorganoditellurides and the immunomodulatory effects of the non-toxic inorganic tellurane, named AS-101, and the plethora of its uses. Inasmuch, the nascent applications of organic telluranes (organotelluranes) as protease inhibitors and its applications in disease... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antitumorals; Biological applications; Enzyme inhibitors; Organotellurium; Proteases; Tellurium. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300006 |
| |
|
| |
|
|
| Tiago,Patrícia Vieira; Silva,Ricardo José da. |
| Cigarrinhas são as principais pragas da pastagem e da cana-de-açúcar, destacando-se os gêneros Deois e Mahanarva. Essas pragas vêm causando sérios danos a estas culturas na região de Tangará da Serra, MT, Brasil. O fungo Metarhizium anisopliae é considerado um eficiente agente para o controle biológico de insetos, possuindo vários fatores de virulência, incluindo a produção de proteases, consideradas fundamentais no processo de penetração através da cutícula do inseto. Este trabalho tem como objetivos analisar a produção de proteases extracelulares a partir de isolados de M. anisopliae, utilizando substratos cuticulares e não-cuticulares, e verificar a influência desses substratos na expressão dessas enzimas. Foram utilizados 4 isolados de M. anisopliae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungos entomopatogênicos; Proteases; Mahanarva fimbriolata; Deois flavopicta. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000100005 |
| |
|
| |
|
|
| Frías-Quintana,Carlos Alfonso; Álvarez-González,Carlos Alfonso; Guerrero-Zárate,Rocío; Valverde-Chavarría,Silvia; Ulloa-Rojas,Juan B.. |
| ABSTRACT Wolf cichlid, Parachromis dovii, is a species with a high potential for aquaculture in Central America; however, the knowledge of the digestive physiology in larvae period is limited. For these reason, this study evaluated the changes on digestive enzymes (alkaline and acid proteases, trypsin, chymotrypsin, aminopeptidase, carboxypeptidase, lipases, amylases, and phosphatases) during early ontogeny by biochemical analysis. All digestive enzymes were detected at first feeding (6 days after hatching, DAH, 9.49 mm, 168 degree-days DD). Afterwards all enzymes reached two main peaks in activity at 14 or 22 DAH (15.10 mm, 364 DD and 20.83 mm, 550 DD, respectively). Later, there was a gradual decrease in activity for trypsin and acid and alkaline... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Larviculture; Proteases; Zymogram. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100215 |
| |
|
| |
|
|
| Frías-Quintana,Carlos Alfonso; Peña-Marín,Emyr Saul; Ramírez-Custodio,Carlos David; Martínez-García,Rafael; Jiménez-Martínez,Luis Daniel; Camarillo-Coop,Susana; Guerrero-Zárate,Rocío; Asencio-Alcudia,Gloria Gertrudys; Álvarez-González,Carlos Alfonso. |
| ABSTRACT In the Southeast of Mexico, there are many native cichlids with commercial interest such as redhead cichlid (Vieja melanurus) and twoband cichlid (V. bifasciata), which have a great local demand and excellent meat quality. However, it is necessary to implement their culture based on nutrition studies and digestive biochemistry. This study’s objective was to characterize these two cichlids’ digestive proteases (pH, temperature, and inhibitors) through biochemistry techniques. Results showed that V. melanurus and V. bifasciata have a digestive capacity analogous to other omnivore fishes, where the optimal pH values of stomach proteases (4 and 2, respectively) and intestinal proteases (6 and 12, respectively), the optimal temperature of acid (35°C... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inhibitors; PH; Proteases; Stability; Temperature. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100210 |
| |
|
|
| Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto. |
| Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009 |
| |
|
| |
|
| |
|
| |
|
|
| Tebaldi,Victor Maximiliano Reis; Oliveira,Thales Leandro Coutinho de; Boari,Cleube Andrade; Piccoli,Roberta Hilsdorf. |
| O isolamento e a identificação de microrganismos em leite cru se tornam interessantes do ponto de vista de saúde pública, pois dependendo das espécies isoladas, ações direcionadas podem ser tomadas visando a melhoria de sua qualidade. A deterioração do leite é conseqüência sobretudo do crescimento de microrganismos psicrotróficos, que produzem lipases e proteases termoestáveis que não são desnaturadas durante o processo de pasteurização, conferindo sabores e odores rançoso e amargo, respectivamente. Assim, o objetivo deste trabalho foi isolar e identificar os principais gêneros de bactérias pertencentes à família Enterobacteriaceae, Gram-negativas oxidase positiva, gêneros Staphylococcus e Enterococcus, bem como atividade de lipases e proteases de 16... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leite cru; Identificação bacteriana; Actérias mesofílicas; Lípases; Proteases. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000300036 |
| |
|
|
| Bordusa,F.. |
| This article reports on the design and characteristics of substrate mimetics in protease-catalyzed reactions. Firstly, the basis of protease-catalyzed peptide synthesis and the general advantages of substrate mimetics over common acyl donor components are described. The binding behavior of these artificial substrates and the mechanism of catalysis are further discussed on the basis of hydrolysis, acyl transfer, protein-ligand docking, and molecular dynamics studies on the trypsin model. The general validity of the substrate mimetic concept is illustrated by the expansion of this strategy to trypsin-like, glutamic acid-specific, and hydrophobic amino acid-specific proteases. Finally, opportunities for the combination of the substrate mimetic strategy with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioorganic chemistry; Enzyme catalysis; Peptide synthesis; Proteases; Substrate mimetics. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500001 |
| |
|
|
| Nascimento,Wellingta Cristina Almeida do; Silva,Camila Rocha da; Carvalho,Raquel Vieira de; Martins,Meire Lelis Leal. |
| Bacillus sp. SMIA-2 cresceu e secretou proteases quando cultivado em culturas líquidas contendo citrato trissódico como fonte de carbono e nitrato de amônio como fonte de nitrogênio. A atividade máxima da enzima foi alcançada após 8 horas de incubação do microrganismo, com níveis de 7,2 U.mg -1 proteína. A suplementação do meio com 2,0 mM de CaCl2 não proporcionou um aumento da atividade da protease, porém aumentou a sua estabilidade de 2 para 4 horas. A redução da concentração do fosfato de potássio no meio de cultura de 11,0 para 5,0 mM promoveu um acréscimo de 82% (13 U.mg -1 proteína) na atividade da protease. A substituição do nitrato de amônio presente no meio de cultura por 0,1% de soro de queijo aumentou a atividade da protease para 25 U.mg -1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteases; Bacillus sp.; Água de maceração de milho; Soro de queijo. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000200035 |
| |
|
|
| Merino-Contreras,María L.; Sánchez-Morales,Froylán; Jiménez-Badillo,María L.; Peña-Marín,Emyr S.; Álvarez-González,Carlos A.. |
| ABSTRACT Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Inhibitors; Pepsin; Proteases; Zymogram. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400206 |
| |
|
|
| Guzmán,Fanny; Barberis,Sonia; Illanes,Andrés. |
| Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis... |
| Tipo: Journal article |
Palavras-chave: Enzymatic synthesis; Peptides; Proteases; Solid-phase synthesis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012 |
| |
|
| |
|
| |
|
| |
| Registros recuperados: 27 | |
|
|
|
