|
|
|
|
| |
|
| |
|
| |
|
|
| Duarte,M.C.; Babeto,E.; Leite,K.R.M.; Miyazaki,K.; Borim,A.A.; Rahal,P.; Silva,A.E.. |
| The objective of this study was to determine the levels of TERT mRNA and TERT protein expression in stomach precancerous lesions such as intestinal metaplasia (IM) and gastric ulcer (GU) and compare them to gastric cancer (GC). Real-time PCR was performed to detect TERT mRNA expression levels in 35 biopsies of IM, 30 of GU, and 22 of GC and their respective normal mucosas. TERT protein was detected by immunohistochemistry in 68 samples, 34 of IM, 23 of GU, and 11 of GC. Increased TERT mRNA expression levels were observed in a significant number of cases, i.e., 46% of IM, 50% of GU, and 79% of GC. The relative mean level of TERT mRNA after normalization with the β-actin reference gene and comparison with the respective adjacent normal mucosa was slightly... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Gastric ulcer; Intestinal metaplasia; Gastric cancer; TERT; Gene expression; Protein expression. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200003 |
| |
|
| |
|
| |
|
|
| Xu,Xiaochun; Wei,Xuan; Yang,Yuxin; Niu,Wenzhi; Kou,Qifang; Wang,Xiaolong; Chen,Yulin. |
| Background The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein expressions of PPARγ, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR) and Western blot. Results The tail measurements increased with age. We observed no significant differences (P > 0.05) of PPARγ mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS... |
| Tipo: Journal article |
Palavras-chave: Fat-tailed sheep; MRNA transcription; Protein expression; Transcription factor and enzyme genes. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200010 |
| |
|
|
| Manzur,María J.; Muñoz,Rosana V.; Lucero,Adrián A.; Juri Ayub,Maximiliano; Alvarez,Sergio E.; Ciuffo,Gladys M.. |
| For biotechnological purposes, protein expression refers to the directed synthesis of large amounts of desired proteins. The aim of the present work was to produce reverse transcriptase Moloney murine Leukaemia Virus retro-transcriptase and Taq DNA polymerase, as bioactive products. In the present paper, we report the preparation of recombinant enzymes, expressed in E. coli strains. The enzymes produced exhibited quite good activity, compared with commercial enzymes, allowing us to replace the last ones for several lab applications. We are reporting changes and modifications to standard protocols described. The standard protocols were modified, i.e. for the purification step of Taq, a temperature dependent procedure was designed. The enzymes produced were... |
| Tipo: Journal article |
Palavras-chave: Bioactivity; Protein expression; Purification; Recombinant enzymes. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300023 |
| |
|
|
|
