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Involvement of Src tyrosine kinase and protein kinase C in the expression of macrophage migration inhibitory factor induced by H2O2 in HL-1 mouse cardiac muscle cells BJMBR
Rao,F.; Deng,C.Y.; Zhang,Q.H.; Xue,Y.M.; Xiao,D.Z.; Kuang,S.J.; Lin,Q.X.; Shan,Z.X.; Liu,X.Y.; Zhu,J.N.; Yu,X.Y.; Wu,S.L..
Macrophage migration inhibitory factor (MIF), a pleiotropic cytokine, plays an important role in the pathogenesis of atrial fibrillation; however, the upstream regulation of MIF in atrial myocytes remains unclear. In the present study, we investigated whether and how MIF is regulated in response to the renin-angiotensin system and oxidative stress in atrium myocytes (HL-1 cells). MIF protein and mRNA levels in HL-1 cells were assayed using immunofluorescence, real-time PCR, and Western blot. The result indicated that MIF was expressed in the cytoplasm of HL-1 cells. Hydrogen peroxide (H2O2), but not angiotensin II, stimulated MIF expression in HL-1 cells. H2O2-induced MIF protein and gene levels increased in a dose-dependent manner and were completely...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Macrophage migration inhibitory factor; HL-1 cells; Hydrogen peroxide; Atrial fibrillation; Protein kinases.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900746
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Regulation of gap junctions by protein phosphorylation BJMBR
Sáez,J.C.; Martínez,A.D.; Brañes,M.C.; González,H.E..
Gap junctions are constituted by intercellular channels and provide a pathway for transfer of ions and small molecules between adjacent cells of most tissues. The degree of intercellular coupling mediated by gap junctions depends on the number of gap junction channels and their activity may be a function of the state of phosphorylation of connexins, the structural subunit of gap junction channels. Protein phosphorylation has been proposed to control intercellular gap junctional communication at several steps from gene expression to protein degradation, including translational and post-translational modification of connexins (i.e., phosphorylation of the assembled channel acting as a gating mechanism) and assembly into and removal from the plasma membrane....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gap junctions; Connexins; Protein phosphorylation; Sites of phosphorylation; Protein kinases; Cell-to-cell communication.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500001
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Restoration of sensory dysfunction following peripheral nerve injury by the polysaccharide from culinary and medicinal mushroom, Hericium erinaceus (Bull.: Fr.) Pers. through its neuroregenerative action Ciênc. Tecnol. Aliment.
WONG,Kah-Hui; KANAGASABAPATHY,Gowri; BAKAR,Robiah; PHAN,Chia-Wei; SABARATNAM,Vikineswary.
Abstract Peripheral nerves have the unique capability to regenerate after injury. Insights into regeneration of peripheral nerves after injury may have implications for neurodegenerative diseases of the nervous system. We investigated the ability of polysaccharide from Hericium erinaceus mushroom in the treatment of nerve injury following peroneal nerve crush in Sprague-Dawley rats by daily oral administration. In sensory functional recovery test, the time taken for the rats to withdraw its hind limb from contact with the hot plate was measured. The test revealed acceleration of sensory recovery in the polysaccharide group compared to negative controls. Further, peripheral nerve injury leads to changes at the remotely located DRG containing cell bodies of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hericium erinaceus; Polysaccharide; Peripheral nerve regeneration; Sensory functional recovery; Protein kinases; Blood-nerve barrier.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000400712
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