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Registros recuperados: 9
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Comparison of protein profile of co-existing Fasciola hepatica and Fasciola gigantica parasite in Bos taurus (cattle) and Bubalus bubalis (Philippine water buffalo) OAK
De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G.
Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host...
Palavras-chave: Cattle & carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382
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Desication sensitivity from different coffee seed phenological stages. Repositório Alice
SANTOS, F. C.; ROSA, S. D. V. F. da; VON PINHO, E. V. de R.; CIRILLO, M. A.; CLEMENTE, A. da C. S..
Maturity stage and drying method are the factors that most influence coffee seed quality. The objective of this study was to assess the physiological quality and investigate the electrophoretic patterns of catalase and endo-ß-mannanaseenzymes and heat resistant proteins in coffee seeds harvested at different phenological stages and dried under different conditions. Physiological quality was assessed when the seeds had developed the green, greenish-yellow, cherry, overripe and dry stages after three treatments: no drying, conventional drying and fast drying. After each treatment, the physiological quality of the seeds was assessed using the germination test and electrophoretic patterns of heat resistant proteins and the activity of catalase and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Seed drying; Physiological quality; Protein profile; Qualidade fisiológica; Pefil proteico; Coffea arabica; Secagem; Coffea arabica.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1039773
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Protein profile analysis by SDS-PAGE of Mycoplasma Gallisepticum strains S6(208) and F-K810 grown in Hayflick's and Frey's media BJM
Ferraz,Priscila N.; Menezes,Claudio C. P.; Danelli,Maria das Graças M.; Lizeu,Jackeline de O. P.; Nascimento,Elmiro R. do; Lucchesi,Daniela M..
The Mycoplasma gallisepticum strains [wild-type S6 (208) and a vaccine-type F-K810] grown in Frey´s and Hayflick´s media were analyzed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mycoplasma gallisepticum; SDS-PAGE; Protein profile.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200010
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Protein profile in freeze-dried chicken embryo eggs with different periods of development Braz. J. Vet. Res. Anim. Sci.
Campos,Célia Maria Teixeira de; Hamad,Antônio José Simões; Amante,Edna Regina; Thapon,Jean-Louis; Nau,Françoise; Guerin-Dubiard,Catherine.
This article presents the protein profile in freeze-dried chicken embryo eggs with different development periods (0, 3, 5, 7, 9, 11 days). The protein profile was determined through HPLC-reverse phase, electrophoresis SDS-PAGE and IFE-electrophoresis. Protein profile of these eggs changes according to the period of development and the change is more evident after the 5th day, where there was an increase in ovalbumin, ovotransferrin, apoLDL, apoHDL and lysozyme concentration, and a decrease in ovomucin and ovostatin concentration. The hypothesis that the change in the protein profile is due to protein biosynthesis and disintegration that happens during incubation process is discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo eggs; Protein profile.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000700001
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Proteomic analysis of developing somatic embryos of Coffea Arabica. Repositório Alice
TONIETTO, A.; SATO, J. H.; TEIXEIRA, J. B.; SOUZA, E. M. de; PEDROSA, F. O.; FRANCO, O. L.; REIS, A. M. dos.
bitstream/item/156256/1/art3A10.10072Fs11105-012-0425-7.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: 2-DE; Protein profile; Molecular markers; Mass spectrometry; Somatic embryogenesis.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/950104
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Quality of horse F(ab′)2 antitoxins and antirabies immunoglobulins: protein content and anticomplementary activity J. Venom. Anim. Toxins incl. Trop. Dis.
Squaiella-Baptistão,Carla Cristina; Magnoli,Fábio Carlos; Marcelino,José Roberto; Sant’Anna,Osvaldo Augusto; Tambourgi,Denise V..
Abstract Background Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and on the quality control are necessary to reduce the amount of protein aggregates. These may trigger adverse reactions in patients by activating the complement system and inducing the generation of anaphylatoxins. Herein, we used immunochemical methods to predict the quality of horse F(ab′)2 anti-botulinum AB, anti-diphtheric, antitetanic and anti-rabies immunoglobulins, in terms of amount of proteins and protein aggregates. Methods Samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heterologous immunoglobulin; F(ab′)2 fragment; Antitoxins; Anti-rabies; Protein profile; Complement system.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100309
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Reserve metabolism of stored and germinated Araucaria angustifolia seeds Agronomy
Araldi, Cristhyane Garcia; Coelho, Cileide Maria Medeiros.
Germination metabolism of recalcitrant seeds of Araucaria angustifolia is activated in storage, which complicates the seeds conservation and utilization. This study aimed to identify the changes in the reserve metabolites of A. angustifolia seeds throughout storage in order to understand the processes of hydrolysis caused by germination metabolism. Mature seeds were harvested in southern Brazil and stored in an ambient environment and cold chamber conditions. Biochemical analyses were performed for embryos and megagametophytes from seeds stored for 0, 15, 30, 45, and 90 days. Due to seeds being in advanced germination in storage, they were evaluated at 90 days in different early developmental categories: I – seeds with mature embryos, II – seeds with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian pine; Conifer; Protein profile; Reserve mobilization..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42707
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Soil drying and rewatering applied at three grain developmental stages affect differentially growth and grain protein deposition in wheat (Triticum aestivum L.) Braz. J. Plant Physiol.
Beltrano,José; Ronco,Marta Guillermina; Arango,María Cecilia.
Water deficits cause large yield losses in wheat. Although anthesis is generally considered the most vulnerable period, water deficit during grain filling can also cause yield losses. The objective of this study was to investigate the effect of water stress and rewatering, at three different grain developmental stages, on physiological and grain filling parameters and on yield components. Wheat plants were subjected to water deficit and rewatering at the watery ripe, milk and soft dough stages. In the flag leaf, water stress decreased the relative water content, the chlorophyll and protein content and increased the leakage of solutes, at all three studied grain filling stages. Water stress at the watery ripe and milk stages reduced the final grain dry mass...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Grain filling; Growth rate; Logistic model; Protein profile; Water stress.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200011
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Study of comparative proteome between normal and inverted karyotypes of human mesenchymal stem cells Biological Sciences
de Souza Santos, John Lenon; Câmara, Alice Barros; Meira, Isabella Tanus Job e; Oliveira, Jonas Ivan Nobre.
 Multipotent mesenchymal stem cells have been expanded in vitro for cellular therapy in numerous clinical settings without standardized culture conditions or quality-control schemes. The in vitro expansion is necessary to obtain sufficient cells for clinical applications. However, the expansion may induce genetic and functional abnormalities which may affect the safety and functionality of MSC, especially the chromosomal stability. This study aimed to investigate the protein profile of umbilical cord-derived MSC with normal and inverted karyotypes after expansion in the laboratory. Mass spectrometry analysis was performed and the Bradford method, Scaffold software, String and Cytoscape databases were employed to measure and characterize the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic instability; Protein profile; Umbilical cord; Interaction network. genetic instability; Protein profile; Umbilical cord; Interaction network..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/50260
Registros recuperados: 9
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