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Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco. |
Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005 |
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Chaves,Guilherme Maranhão; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo. |
In order to evaluate the pathogenicity of yeasts of medical importance, 15 strains stocked in mineral oil at the URM Culture Collection of the Department of Mycology, Biological Sciences Centre, Federal University of Pernambuco and 15 fresh strains isolated from AIDS patients interned at the Clinical Hospital, Centre of Health Sciences, Federal University of Pernambuco, were selected. As pathogenicity characteristics, the ability to grow at 37ºC and production of phospolipase and proteinase were tested. All samples grew at 37ºC and exhibited proteinase activity. However when tested on solid medium, the proteinase activity was negative. Among 15 stocked samples, 13 (86.66%) were phospholipase positive and two (13.34%) were phospholipase negative. From the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yeasts; Pathogenicity; Temperature; Phospholipase; Proteinase. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300003 |
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Ribeiro, Ana Paula de Oliveira; UFV; Otoni, Wagner Campos; UFV; Picanço, Marcelo Coutinho; Universidade Federal de Viçosa, Centro de Ciências Biológicas e da Saúde; Galvan, Tederson Luiz; UFV; Pereira, Eliseu José Guedes; UFV; Picoli, Edgard Augusto de Toledo; UFV; Silva, Derly José Henriques da; UFV. |
Esta pesquisa objetivou avaliar a resistência da berinjela (Solanum melongena) transgênica, contendo o gene orizacistatina, a Mechanitis polymnia L. e Mechanitis lysimnia Fabr (Lepidoptera: Nymphalidae). Foram avaliadas a área foliar consumida, a mortalidade e duração das fases larval e pupal de M. polymnia e M. lysimnia. A berinjela transgênica não apresentou resistência aos Lepidoptera M. polymnia e M. lysimnia |
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Palavras-chave: 5.01.02.00-1 Fitossanidade Solanum melongena; Lepidoptera; Proteinase; Biotecnologia; Resistência de plantas 5.01.02.00-1 Fitossanidade. |
Ano: 2005 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1345 |
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