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Registros recuperados: 9
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Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children BJM
Mardegan,Rita de Cássia; Klein,Marlise Inêz; Golvea,Magda Baglione; Rodrigues,Janaina Aparecida Oliveira; Gonçalves,Reginaldo Bruno; Höfling,José Francisco.
Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Genotyping; Proteinase; Phospholipase; Virulence.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100005
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Etude de la variabilité spatio-temporelle des caractéristiques physiologiques des jeunes stades de vie de la seiche Sepia officinalis L. en Manche. ArchiMer
Safi, Georges.
The common cuttlefish Sepia officinalis, is a major exploited marine resource in the English Channel. It has strong seasonal and interannual variations in recruitment. Mechanisms affecting the renewal of the stock by the arrival of new “recruits” and their number need to be better understood. Thus the study of the spatial and temporal variability of “pre-recruits” physiological characteristics was conducted in four spawning sites of the Channel [i.e. Agon Coutainville (FR), Baie de Seine (FR), Selsey (UK) and Torbay (UK)]. The studied parameters were the eggs quality and hatching rate then, the digestive and immune performance of juveniles in relation to local environmental conditions. English eggs have a delayed hatching time and higher hatching rate when...
Tipo: Text Palavras-chave: Seiche; Recrutement (biologie); Oeufs--incubation; Enzymes digestives; Protéases; Lysozyme; Écophysiologie; Manche (mer).; Cuttelfish; Recruitment (Population biology); Eggs--incubation; Digestive enzymes; Proteinase; Lysozyme; Ecophysiology; English Channel.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00161/27232/25439.pdf
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Isolation and characterization of a cDNA encoding a serine proteinase from the root-knot nematode Meloidogyne incognita. Repositório Alice
FRAGOSO, R. da R.; BATISTA, J. A. N.; OLIVEIRA NETO, O. B. O.; GROSSI-de-SÁ, M. F..
Tipo: Separatas Palavras-chave: Nematóide de planta; Proteinase; Clonagem; Meloidogyne Incognita.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/185896
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Pathogenicity characteristics of stocked and fresh yeasts strains BJM
Chaves,Guilherme Maranhão; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo.
In order to evaluate the pathogenicity of yeasts of medical importance, 15 strains stocked in mineral oil at the URM Culture Collection of the Department of Mycology, Biological Sciences Centre, Federal University of Pernambuco and 15 fresh strains isolated from AIDS patients interned at the Clinical Hospital, Centre of Health Sciences, Federal University of Pernambuco, were selected. As pathogenicity characteristics, the ability to grow at 37ºC and production of phospolipase and proteinase were tested. All samples grew at 37ºC and exhibited proteinase activity. However when tested on solid medium, the proteinase activity was negative. Among 15 stocked samples, 13 (86.66%) were phospholipase positive and two (13.34%) were phospholipase negative. From the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeasts; Pathogenicity; Temperature; Phospholipase; Proteinase.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300003
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Presence of Staphylococcus spp. and Candida spp. in the human oral cavity BJM
Martins,Clélia Aparecida de Paiva; Koga-Ito,Cristiane Yumi; Jorge,Antonio Olavo Cardoso.
The presence of yeasts and staphylococci in the oral cavity is important because they can act as supplementary microbiota and in certain situations can cause oral or systemic diseases. The aim of this work was to study the prevalence of Candida spp. and Staphylococcus spp. in the human oral cavity. Oral rinses were collected from sixty-eight individuals according to the technique described by Samaranayake and MacFarlane and then cultured on Sabouraud medium supplemented with chloramphenicol and Baird-Parker agar. After the incubation period, the microorganisms were isolated and identified through biochemical tests. The data obtained were statistically analysed by ANOVA. Candida spp. were isolated from 61.76% of the examined individuals and C. albicans was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Candida; Phospholipase; Proteinase; Killer system; Oral cavity.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300009
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Proregion of Acanthoscelides obtectus cysteine proteinase: a novel peptide with enhanced selectivity toward endogenous enzymes. Repositório Alice
SILVA, F. B.; MONTEIRO, A. C. S.; DEL SARTO, R. P.; MARRA, B. M.; DIAS, S. C.; FIGUEIRA, E. L. Z.; OLIVEIRA, G. R.; ROCHA, T. L.; SOUZA, D. S. L.; SILVA, M. C. M. da; FRANCO, O. L.; GROSSI DE SA, M. F..
Tipo: Separatas Palavras-chave: Defesa da planta; Propeptide; Proteinase; Acanthoscelides obtectus.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188774
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Resistência de berinjela transgênica contendo o gene orizacistatina a Mechanitis polymnia e Mechanitis lysimnia - DOI: 10.4025/actasciagron.v27i4.1345 Agronomy
Ribeiro, Ana Paula de Oliveira; UFV; Otoni, Wagner Campos; UFV; Picanço, Marcelo Coutinho; Universidade Federal de Viçosa, Centro de Ciências Biológicas e da Saúde; Galvan, Tederson Luiz; UFV; Pereira, Eliseu José Guedes; UFV; Picoli, Edgard Augusto de Toledo; UFV; Silva, Derly José Henriques da; UFV.
Esta pesquisa objetivou avaliar a resistência da berinjela (Solanum melongena) transgênica, contendo o gene orizacistatina, a Mechanitis polymnia L. e Mechanitis lysimnia Fabr (Lepidoptera: Nymphalidae). Foram avaliadas a área foliar consumida, a mortalidade e duração das fases larval e pupal de M. polymnia e M. lysimnia. A berinjela transgênica não apresentou resistência aos Lepidoptera M. polymnia e M. lysimnia
Palavras-chave: 5.01.02.00-1 Fitossanidade Solanum melongena; Lepidoptera; Proteinase; Biotecnologia; Resistência de plantas 5.01.02.00-1 Fitossanidade.
Ano: 2005 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1345
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Screening of entomopathogenic Metarhizium anisopliae isolates and proteomic analysis of secretion synthesized in response to cowpea weevil (Callosobruchus maculatus) exoskeleton. Repositório Alice
MURAD, A. M.; LAUMANN, R. A.; LIMA, T. de A.; SARMENTO, R. B. C.; NORONHA, E. F.; ROCHA, T. L.; VALADARES-INGLIS, M. C.; FRANCO, O. L..
Tipo: Separatas Palavras-chave: Secretome; Two-dimensional gels; Proteinase; Metarhizium Anisopliae; Chitinase.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/188239
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Treatment of serum with supernatants from cultures of Candida albicans reduces its serum-dependent phagocytosis BJM
Santos,Aderbal Antonio dos; Sá,Eglius Alexandre Colignese de; Gaziri,Luis Carlos Jabur; Felipe,Ionice.
Candida albicans is a potent activator of the complement system, and heat labile opsonins produced by activation of C3 (C3b and iC3b) enhance phagocytosis of C. albicans mediated by complement receptors. In this study we treated mouse serum with supernatants from cultures of a protease producer strain of C. albicans and evaluated the ability of this serum to enhance phagocytosis of C. albicans. Cell-free supernatants from cultures of C. albicans were concentrated 5 fold and added to mouse serum for 30 min at 37ºC, before using this serum for opsonization of glutaraldehyde-fixed yeast cells. We observed that normal mouse serum increased about 3 fold the phagocytosis of C. albicans by mice peritoneal macrophages, whereas supernatant-treated serum did not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Phagocytosis; Proteinase.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100016
Registros recuperados: 9
Primeira ... 1 ... Última
 

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