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Registros recuperados: 9
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A practical approach to the understanding and teaching of RNA silencing in plants 69
Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián.
Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach...
Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
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A rapid screening method for grain iron content in Pearl Millet 30
Velu, G..
Palavras-chave: Wood decay; Genotypes; Grain; Millets; Protocols; Flours; Sampling; Iron; Absorption; Potassium.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3210
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Direct somatic embryogenesis and organogenesis pathway of plant regeneration can seldom occur simultaneously within the same explant of sorghum 30
Girijashankar, V..
Palavras-chave: Somatic embryos; Transgenics; Somatic embryogenesis; Auxins; Regeneration; Protocols; Sorghum; Cytokinins; Embryonic development; Transgenic plants.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3300
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Guide de la mensuration des poissons, mollusques, crustacés et mammifères marins en halieutique 5
Badts, Vincent; Bertrand, Jacques.
This guide presents the rules of measurement of fishes, mollusks, shellfishes and marine mammals applicable to the halieutic observations coordinated by lfremer, including the actions driven within the framework of control networks coordinated at the international level, in particular within the European regulation on the data collection regulation (OCR).
Tipo: Text Palavras-chave: Mensuration; Taille; Poissons; Mollusques; Crustacés; Mammifères marins; Halieutique; Protocoles; Measurement; Size; Fishes; Mollusks; Shellfishes; Marine mammals; Halieutic; Protocols.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00503/61477/65257.pdf
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Lessons learned from an intercalibration exercise on the quantification and characterisation of microplastic particles in sediment and water samples 5
Cadiou, Jean-francois; Gerigny, Olivia; Koren, Š.; Zeri, C.; Kaberi, H.; Alomar, C.; Panti, C.; Fossi, M.c.; Adamopoulou, A.; Digka, N.; Deudero, S.; Concato, M.; Carbonell, A.; Baini, M.; Galli, M.; Galgani, Francois.
An intercalibration exercise on the characterisation of microplastics in marine sediment and water samples was carried out among five laboratories involved in the implementation of the Marine Strategy Framework Directive (MSFD) in their country. The samples were prepared by mixing cleaned natural sediment and sea water with microplastics sets made of particles of various polymers, shapes and colours. Overall, the errors on total counts were under 25% in absolute value. The risk of non-detection and loss of particles is greater than the risk of contamination during sample analysis. Significant differences are observed among particle types. It appears difficult to obtain reliable and comparable data on the colour of microplastics. A comparison of the errors...
Tipo: Text Palavras-chave: Microplastics; Intercalibration; Protocols; Sediment; Seawater.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00622/73397/72812.pdf
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Seed viability of active collections in ex-situ genebanks: an analysis of sorghum germplasm conserved at ICRISAT genebank 30
Sastry, D.V.S.S.R..
Palavras-chave: Seed viability; Gene banks; Sorghum; Grain; Germplasm; Regeneration; Relative humidity; Protocols; Seed treatment; Germplasm conservation.
Ano: 2008 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3116
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Standardization of a Protocol to Screen for Salinity Tolerance in Groundnut 30
Vadez, V..
Palavras-chave: Genotypes; Root nodulation; Tolerance; Biomass; Protocols; Leaf area; Groundnuts; Chlorophylls; Field capacity; Tissue analysis.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3263
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Summary proceedings of the ICRISAT/NARS workshop on methods and joint impact targets in Western and Central Africa 30
J..
Palavras-chave: Pigeonpeas; Millets; Protocols; Sorghum; Nutrient management; Cowpeas; Policies; Seed production; Investment; Technology.
Ano: 1996 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4188
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Variation in sensitivity to salinity in groundnut cultivars during seed germination and early seedling growth 30
Singh,R.
Palavras-chave: Viruses; Groundnuts; Tolerance; Vegetative stage; Water; Genotypes; Seed testing; Protocols; Germinability; Irrigation water.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3357
Registros recuperados: 9
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