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Registros recuperados: 11
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Characterization of fusion products from protoplasts of yeasts and their segregants by electrophoretic karyotyping and RAPD Rev. Microbiol.
Martins,Cleide Viviane Buzanello; Horii,Jorge; Pizzirani-Kleiner,Aline Aparecida.
In order to characterize fusion products from yeast protoplasts and their segregants, with important features to the wine making industry, electrophoretic karyotyping and RAPD (Random Amplified Polymorphic DNA) were utilized. Electrophoretic karyotyping was performed by the CHEF ("contour-clamped homogeneous electric field electrophoresis") method, which allowed the detection of chromosomal band complementation in fusion products and the presence of patterns of both parental and intermediary strains in segregants. By utilizing two primers, an amplification pattern of DNA fragments was obtained. While fusion products (diploid) showed a pattern of complementary bands, segregants showed bands of either parental strains or even intermediary bands
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protoplast fusion; Electrophoretic karyotyping; RAPD.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100014
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Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts. Repositório Alice
BONA, C.M. de; GOULD, J.H.; MILLER JR.; J.C.; STELLY, D.; LOUZADA, E.S..
The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus sp.; Gamma irradiation; Grapefruit; Protoplast fusion; Somatic hybridization; Sweet orange; Irradiação gama; Pomelo; Fusão de protoplasto; Hibridação somática; Laranja doce.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/125867
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Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts PAB
Bona,Claudine Maria de; Gould,Jean Howe; Miller Jr,J. Creighton; Stelly,David; Louzada,Eliezer Silva.
The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sp.; Gamma irradiation; Grapefruit; Protoplast fusion; Somatic hybridization; Sweet orange.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500004
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Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum BJM
Varavallo,Maurilio Antonio; Queiroz,Marisa Vieira de; Lana,Taís Guimarães; Brito,Admilson Toscano Ribeiro de; Gonçalves,Daniel Bonoto; Araújo,Elza Fernandes de.
Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protoplast fusion; Pectinases; Penicillium.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100011
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Pathogenicity and its mode of action in different sedentary stages of Heterodera glycines (Tylenchida: Heteroderidae) by Verticillium lecanii hybrid strains OAK
SHINYA, Ryoji; AIUCHI, Daigo; KUSHIDA, Atsuhiko; TANI, Masayuki; KURAMOCHI, Katsuhisa; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 倉持, 勝久; 小池, 正徳.
The current study was conducted to investigate the pathogenicity and its mode of action by Verticillium lecanii hybrid strains in different sedentary stages of Heterodera glycines. Three different sedentary stages (pale yellow female, yellow brown cyst, and dark brown cyst) of H. glycines were treated with V. lecanii and incubated for 3 weeks on water agar. After 3 weeks of incubation, eggs were investigated for the following parameters: (i) the infection frequencies of eggs, (ii) the number of eggs laid, and (iii) the number of mature and healthy eggs. Subsequently, the fecundity of H. glycines treated with V. lecanii was investigated in greater detail. Consequently, some strains had a greater ability to infect eggs and this significantly reduced the...
Palavras-chave: Soybean cyst nematode; Protoplast fusion; Fecundity; Egg laying; Biological control.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2849
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Protoplast fusion as a means of producing new nucleo-cytoplasmic combinations in rice International Rice Research Institute
Villegas, Alicia N..
83 l. : ill. Thesis (M.S.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Hybrids; Protoplast fusion; Varieties.
Ano: 1989 URL: http://hdl.handle.net/123456789/1094
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Screening of Verticillium lecanii (Lecanicillium spp.) Hybrid Strains Based on Evaluation of Pathogenicity against Cotton Aphid and Greenhouse Whitefly, and Viability on the Leaf Surface OAK
相内, 大吾; 馬場, ゆき子; 稲見, 圭吾; 新屋, 良治; 谷, 昌幸; 倉持, 勝久; 堀江, 早弥佳; 小池, 正徳.
In a previous study, protoplast fusion was performed among 3 strains of the entomopathogenic fungi Verticillium lecanii, as a result of which 174 hybrid strains were obtained. The aim of the present study was to screen desirable hybrid strains that have a wide host range or durable effects. Initially, 43 hybrid strains were used in bioassays against the cotton aphid. Of these strains, 30 induced equal or higher mortality as compared to Vertalec (41.9%). Secondly, 50 hybrid strains were used in bioassays against the greenhouse whitefly. Of these strains, 37 exhibited an equal or higher infection rate as compared to that of Mycotal (36.2%). Finally, 50 hybrid strains were applied to cucumber leaves in order to test strain viability under low humidity...
Palavras-chave: Biological control; Lecanicillium spp; Protoplast fusion; Screening; Verticillium lecanii.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2587
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Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck . Repositório Alice
CALIXTO, M.C.; MOURÃO FILHO, F. de A.A.; MENDES, B.M.J.; VIEIRA, M.L.C..
This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genetic improvement; Protoplast fusion; Rootstock; Melhoramento genético; Fusão de protoplastos; Porta-enxerto.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/112201
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Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck PAB
Calixto,Marcia Cristina; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Vieira,Maria Lúcia Carneiro.
This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic improvement; Protoplast fusion; Rootstock.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000700015
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Somatic hybridization by electrofusion of banana protoplasts. Repositório Alice
MATSUMOTO, K.; VILARINHOS, A. D.; OKA, S..
bitstream/item/177828/1/ID-240621.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Protoplasto fusão; Huibrido Embryogenesis; Somatic hybrid; Banana; Embriogénese; Musa; Protoplast fusion.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/176785
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Variation in growth at different temperatures and production and size of conidia in hybrid strains of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) OAK
AIUCHI, Daigo; BABA, Yukiko; INAMI, Keigo; SHINYA, Ryoji; TANI, Masayuki; KOIKE, Masanori; 相内, 大吾; 谷, 昌幸; 小池, 正徳.
In a previous study, we confirmed alterations in several fungal traits in hybrid strains of Verticillium lecanii generated via protoplast fusion. In the present study, we surveyed radial colony growth in different temperature regimes as well as conidia productivity and conidia size of hybrid strains of V. lecanii to elucidate the various primary fungal properties of these strains and to determine the correlation between these properties and virulence to insect pests. Minimum temperature thresholds for all tested strains (except Vertalec®) were below 5°C. Optimal temperatures for colony growth were 20–25°C. Colony growth at high temperature was good or poor depending on the genotype. Temperature and strain significantly affected colony growth. Conidial...
Palavras-chave: Lecanicillium spp; Phenotypic variation; Protoplast fusion; Temperature growth response; Verticillium lecanii.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2848
Registros recuperados: 11
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