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Adhesion and production of degrading enzymes by bacteria isolated from biofilms in raw milk cooling tanks Ciênc. Tecnol. Aliment.
Flach,Juliana; Grzybowski,Valeska; Toniazzo,Geciane; Corção,Gertrudes.
Biofilms in milk cooling tanks compromise product quality even on farms. Due to the lack of studies of this topic, this study evaluated the microbiological conditions of raw milk cooling tanks on farms and characterized the microorganisms isolated from these tanks. Samples were wiped off with sterile swabs from seven milk cooling tanks in three different points in each tank. Mesophiles and psychrotrophic counts were performed in all samples. The isolation of Pseudomonas spp., Bacillus cereus and atypical colonies formed on selective media were also performed, totalizing 297 isolates. All isolates were tested for protease and lipase production and biofilm formation. Of the total isolates, 62.9% produced protease, 55.9% produced lipase, and 50.2% produced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psychrotrophic bacteria; Cleaning process; Lipase; Protease.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000300020
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Detection of airborne psychrotrophic bacteria and fungi in food storage refrigerators BJM
Altunatmaz,Sema Sandikci; Issa,Ghassan; Aydin,Ali.
The purpose of this study was to determine the microbiological air quality (psychrotrophic bacteria and airborne fungi) and distribution of fungi in different types of ready-to-eat (RTE) food-storage refrigerators (n=48) at selected retail stores in the city of Edirne, Turkey. Refrigerators were categorized according to the type of RTE food-storage: meat products, vegetables, desserts, or a mix of food types. Microbiological quality of air samples was evaluated by using a Mas-100 Eco Air Sampler. Four refrigerators (all containing meat products, 8.3%) produced air samples with undetectable microorganisms. The highest detected mean value of airborne psychrotrophic bacteria and fungi was 82.3 CFU/m³ and 54.6 CFU/m³, respectively and were found in mixed-food...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Airborne fungi; Psychrotrophic bacteria; Ready-to-eat food; Air sampler; Refrigerator.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400027
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Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese Ciênc. Tecnol. Aliment.
Frozi,Jesieli Braz; Domingues,Josiane Roberto; Esper,Luciana Maria Ramires; Corrêa da Rosa,Joel Maurício; Silva,Ana Luiza Sant’Anna da Costa; Gonzalez,Alice Gonçalves Martins.
Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains (isolated by cattle’s faeces and a reference strain, EDL933), were inoculated into pasteurized milk (102 and 103 cells.mL–1) to prepare the Minas frescal cheese. As control was used uninfected milk. Physicochemical and microbiological analyses were performed to milk and elaborated cheese. The O157:H7 strains were quantified in the stages of cheese processing and during 0, 2, 4, 5, 7, 10 and 15 storage days at 8 °C onto Sorbitol MacConkey Agar supplemented with potassium tellurite and cefixime (CT-SMAC). O157:H7 was not present in the pasteurised milk prior to the artificial inoculation. At the end of the processing the cheese had 10 to 100 times more STEC O157:H7 than the initial inoculum....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheese; Psychrotrophic bacteria; Foodborne disease.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100108
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