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| Registros recuperados: 34 | |
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| Campos,M.P.; Madeira,M.F.; Silva,D.A.; Solcà,M.S.; Espíndola,O.M.; Mendes Júnior,A.A.V.; Figueiredo,F.B.. |
| ABSTRACT The purpose of the present work was to evaluate the accuracy of quantitative polymerase chain reaction (qPCR) performed on samples of fresh frozen tissue (FT) and formalin-fixed, paraffin-embedded (FFPE) healthy skin. This is a validation study conducted with samples from 46 dogs from an endemic area in Brazil. After sample collection, DNA extractions were conducted using commercial kits and qPCR was oriented to kinetoplast DNA (kDNA) targets of the Leishmania infantum species. The results obtained for the FFPE samples showed 63.6% sensitivity and 77.1% specificity, whereas those obtained for the FT samples showed 100% and 48.6%, respectively. Poor agreement was observed for the results of the qPCR technique with FT and FFPE samples. Our results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnosis; QPCR; Canine visceral leishmaniasis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601443 |
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| Rouze, Heloise; Lecellier, Gael J.; Saulnier, Denis; Planes, Serge; Gueguen, Yannick; Wirshing, Herman H.; Berteaux-lecellier, Veronique. |
| The adaptative bleaching hypothesis (ABH) states that, depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to “switch” or “shuffle” their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community and, thus, contribute to the coral’s overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations and to detect low levels of coral-associated Symbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional molecular methods, to assess the presence of... |
| Tipo: Text |
Palavras-chave: Corals; French polynesia; Clade B; Symbiodinium; QPCR; Flexibility; Generalist; Faithful clade. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00365/47652/47683.pdf |
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| Roué, Mélanie; Smith, Kirsty F.; Sibat, Manoella; Viallon, Jérôme; Henry, Kévin; Ung, André; Biessy, Laura; Hess, Philipp; Darius, Hélène Taiana; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed... |
| Tipo: Text |
Palavras-chave: Ciguatera monitoring; Gambierdiscus; Ciguatoxins; SPATT passive samplers; HP20 resin; CBA-N2a; LC-MS; MS; WS artificial substrate; QPCR; HTS metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74143/73670.pdf |
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| Torres,Mariana de Medeiros; Almeida,Arleana do Bom Parto Ferreira de; Sorte,Eveline da Cruz Boa; Paula,Daphine Ariadne Jesus de; Oliveira,Anderson Castro Soares de; Pescador,Caroline Argenta; Mendonça,Adriane Jorge; Nakazato,Luciano; Sousa,Valéria Régia Franco. |
| A leishmaniose visceral canina é uma doença grave e a morte ocorre por falência renal, considerando que os métodos diagnósticos convencionais não possibilitam a classificação clínica do animal. O objetivo deste estudo foi associar a carga parasitária renal aos achados clínicos e histopatológicos em cães com leishmaniose visceral. A análise microscópica revelou predomínio de nefrite intersticial mononuclear de graus variados em 59,3% dos cães avaliados. Entretanto, não houve diferença entre a carga parasitária renal de sintomáticos e oligossintomáticos (P= 0,35). As lesões renais foram de ordem inflamatória e a quantidade de parasitos não influenciaram na característica dessas lesões e nem nas alterações bioquímicas, mesmo em cães com diferentes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rim; QPCR; Cão; Leishmania sp.. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000500023 |
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| Jorquera,Esteban; Morales,Paz; Tapia,David; Torres,Pamela; Eissler,Yoanna; Espinoza,Juan C; Conejeros,Pablo; Kuznar,Juan. |
| Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to determine the conservation of the primer-binding regions used in polymerase chain reaction (PCR)-based diagnostic methods proposed in the literature. Thus, the robustness of each technique was studied, particularly the eventual effect of further mutations within the primer-binding sites. Results: On analysis, most methods currently used to detect Chilean IPNV varieties were deemed adequate. However, the primers were designed to be genogroup specific, implying that most detection methods pose some risk of detecting all strains prevalent in... |
| Tipo: Journal article |
Palavras-chave: IPNV detection; Mismatch's Tm analysis; QPCR. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200005 |
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| Díaz-asencio, Lisbet; Clausing, Rachel J.; Vandersea, Mark; Chamero-lago, Donaida; Gómez-batista, Miguel; Hernández-albernas, Joan I.; Chomérat, Nicolas; Rojas-abrahantes, Gabriel; Litaker, Wayne; Tester, Patricia; Diogène, Jorge; Alonso-hernández, Carlos M.; Dechraoui Bottein, Marie-yasmine. |
| In Cuba, ciguatera poisoning associated with fish consumption is the most commonly occurring non-bacterial seafood-borne illness. Risk management through fish market regulation has existed in Cuba for decades and consists of bans on selected species above a certain weight; however, the actual occurrence of ciguatoxins (CTXs) in seafood has never been verified. From this food safety risk management perspective, a study site locally known to be at risk for ciguatera was selected. Analysis of the epiphytic dinoflagellate community identified the microalga Gambierdiscus. Gambierdiscus species included six of the seven species known to be present in Cuba (G. caribaeus, G. belizeanus, G. carpenteri, G. carolinianus, G. silvae, and F. ruetzleri). CTX-like... |
| Tipo: Text |
Palavras-chave: Caribbean; Ciguatoxicity; QPCR; Trophic transfer; Ish; Food safety; Food security; Science-based management; Foodborne disease. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00597/70947/69189.pdf |
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| Resende,M.V.; Lucio,A.C.; Perini,A.P.; Oliveira,L.Z.; Almeida,A.O.; Alves,B.C.A.; Moreira-Filho,C.A.; Santos,I.W.; Hossepian de Lima,V.F.M.. |
| The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05). The percentage of female embryos in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Sperm sexing; Centrifugation; Embryo sexing; QPCR; PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300002 |
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| Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da. |
| ABSTRACT: The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine visceral leishmaniasis; Genital pathology; Immunohistochemistry; QPCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102029 |
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| STAGGEMEIER,RODRIGO; ARANTES,THALITA; CAUMO,KARIN S.; ROTT,MARILISE B.; SPILKI,FERNANDO R.. |
| ABSTRACT Acanthamoeba is the most common free-living environmental amoeba, it may serve as an important vehicle for various microorganisms living in the same environment, such as viruses, being pathogenic to humans. This study aimed to detect and quantify human adenoviruses (HAdV) in Acanthamoebas isolated from water samples collected from swimming pools in the city of Porto Alegre, Southern Brazil. Free-living amoebae of the genus Acanthamoeba were isolated from water samples, and isolates (n=16) were used to investigate the occurrence of HAdVs. HAdV detection was performed by quantitative real-time polymerase chain reaction (qPCR). HAdVs were detected in 62.5% (10/16) of Acanthamoeba isolates, ranging from 3.24x103 to 5.14x105 DNA copies per milliliter... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acanthamoeba; Free-living amoebae; Human adenovirus; QPCR; Swimming pools. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200635 |
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| Moraes,DMC; Duarte,SC; Bastos,TSA; Rezende,CLG; Leandro,NSM; Café,MB; Stringhini,JH; Andrade,MA. |
| Abstract Conventional bacteriology techniques and quantitative polymerase-chain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: QPCR; Eggshells; Albumen; Yolk. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100117 |
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| Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry. |
| Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one... |
| Tipo: Text |
Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares). |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf |
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| Agnew, M. Victoria; Friedman, Carolyn S.; Langdon, Christopher; Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Degremont, Lionel; Dhar, Arun K.; Kirkland, Peter; Dumbauld, Brett; Burge, Colleen A.. |
| Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the... |
| Tipo: Text |
Palavras-chave: OsHV-1; Mu vars; Oyster; Virus; Tolerance; Virulence; QPCR. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00665/77739/79862.pdf |
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| Majidi-Mosleh,A; Sadeghi,AA; Mousavi,SN; Chamani,M; Zarei,A. |
| ABSTRACT The objective of the present study was to evaluate the effects of in ovo infusion of probiotic strains (Bacillus subtilis, Enterococcus faecium, and Pediococcus acidilactici ) on jejunal microbial population and mucin gene expression in broiler chicken. In a completely randomized design, 0.5 ml of mediums containing 107 cfu of different probiotic strains, was administered into amniotic fluid of the 480 Cobb fetus (day 18 of incubation), with four treatments, five replicates with twenty four eggs each. For mucin gene expression, samples from the jejunum were taken on day 21 of incubation and day 3 post-hatch. Microbial profile was determined for total lactobacillus and E. coli by sampling jejunal contents on days 1 and 3 of age. Expression of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In ovo infusion; Jejunum; Microbial population; QPCR; Probiotic. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500097 |
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| Mulero, Stephen; Boissier, Jérôme; Allienne, Jean‐françois; Quilichini, Yann; Foata, Joséphine; Pointier, Jean‐pierre; Rey, Olivier. |
| Under ongoing climate changes, the development of large‐scale monitoring tools for assessing the risk of disease emergence constitutes an urging challenge. This is particularly the case for snail‐borne diseases such as the urogenital bilharziasis that emerged in Corsica and threat European countries. The expansion of this tropical disease mainly relies on the local presence of competent snail hosts such as Bulinus truncatus. Unfortunately, very little is known about the actual repartition of freshwater snails worldwide which makes new emergences difficult to predict. In this study, we developed two ready‐to‐use environmental DNA‐based methods for assessing the distribution of B. truncatus from water samples collected in the field. We used two approaches, a... |
| Tipo: Text |
Palavras-chave: Bulinus truncatus; Corsica; DdPCR; Environmental DNA; Environmental monitoring; QPCR; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00593/70554/68723.pdf |
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| Zhang,Zhen; Wen,Bin; Xu,Yuan; Jiang,En-ze; Liu,Jia-yu; Zhu,Ke-li; Ning,Fang-yong; Du,Zhi-Heng; Bai,Xiu-Juan. |
| Abstract Rabbit with hypercholesterolaemia is an important model for studying cholesterol metabolism disease. This study aimed to evaluate the expression stability of nine reference genes for quantitative PCR (qPCR) analysis in adrenal gland, liver, spleen, and kidney tissue from rabbits with hypercholesterolaemia. In total, 30 male Harbin Large White (HLW) rabbits were fed a normal feed (n = 15) or a high cholesterol feed (n = 15) for 8 weeks to induce hypercholesterolaemia. Nine reference genes were verified by qPCR using cDNA extracted from rabbit tissue samples. For qPCR analysis, reference genes were evaluated using the RefFinder and GeNorm algorithms. Overall, seven rabbits with hypercholesterolaemia were identified based on body weight and total... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Evaluation; Reference genes; QPCR; Rabbits; Hypercholesterolaemia. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100428 |
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| Guimarães-Dias,Fábia; Neves-Borges,Anna Cristina; Viana,Antonio Americo Barbosa; Mesquita,Rosilene Oliveira; Romano,Eduardo; Grossi-de-Sá,Maria de Fátima; Nepomuceno,Alexandre Lima; Loureiro,Marcelo Ehlers; Alves-Ferreira,Márcio. |
| Metabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Drought resistance; QPCR; Metabolic pathway; Bioinformatics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200004 |
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| Registros recuperados: 34 | |
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