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Registros recuperados: 16
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Anastomosis groups and molecular variation in Pseudocercospora griseola. Repositório Alice
SILVA, K. J. D. e; SOUZA, E. A.; FREIRE, C. N. S.; ISHIKAWA, F. H..
The fungus Pseudocercospora griseola is the causal agent of angular leaf spot, a common bean (Phaseolus vulgaris L.) disease. The successful development of angular leaf spot-resistant cultivars depends on understanding the levels of variability in pathogen populations. P. griseola shows great pathogenic and genetic variation using inoculation on differential bean cultivars and molecular markers. Nevertheless, how this variability develops is not clearly understood. Parasexuality has been presented as a mechanism used by asexual fungi to increase variation. Hyphal fusion is the first step for the parasexual cycle, and it can be considered an additional trait for population studies. The aim of this study was to identify hyphal fusion (anastomosis) among P....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Feijoeiro comum; Ciclo parassexual; Mancha angular; Marcador molecular; Angular leaf spot; Common bean; RAPD markers; Parasexual cycle; Phaeoisariopsis griseola.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1030017
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Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers Genet. Mol. Biol.
Silveira,Sheila Recepute; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Sera,Tumoru; Carvalho,Valdemar de Paula; Coelho,Alexandre Siqueira Guedes.
The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee breeding; Genetic variability; Molecular variance; RAPD markers.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018
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Description and analysis of genetic diversity among squash accessions BABT
Tsivelikas,Athanasios L.; Koutita,Olga; Anastasiadou,Anastasia; Skaracis,George N.; Traka-Mavrona,Ekaterini; Koutsika-Sotiriou,Metaxia.
In this work, the part of the squash core collection, maintained in the Greek Gene Bank, was assessed using the morphological and molecular data. Sixteen incompletely classified accessions of the squash were characterized along with an evaluation of their resistance against two isolates of Fusarium oxysporum. A molecular analysis using Random Amplified Polymorphic DNA (RAPD) markers was also performed, revealing high level of polymorphism. To study the genetic diversity among the squash accessions, a clustering procedure using Unweighed Pair Group Method and Arithmetic Average (UPGMA) algorithm was also adopted. Two independent dendrograms, one for the morphophysiological and one for molecular data were obtained, classifying the accessions into two and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cluster analysis; Gower's distance; Morphophysiological characteristics; Principal Component Analysis; RAPD markers; Winter squash.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200003
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Distância genética entre linhagens avançadas de germoplasma de algodão com uso de marcadores de RAPD e microssatélites. Repositório Alice
MENEZES, I.P.P. de; HOFFMANN, L.V.; ALVES, M.F.; MORELLO, C. de L.; BARROSO, P.A.V..
O objetivo deste trabalho foi selecionar coeficientes de similaridade para serem aplicados a conjuntos de genótipos de algodoeiro com baixa diversidade genética. Analisou-se um conjunto de 65 linhagens e 4 cultivares de algodão, com marcadores de RAPD e SSR, e estimou-se a similaridade genética de acordo com sete coeficientes de similaridade: Coincidência Simples, Rogers & Tanimoto, Ochiai, Hamman, Jaccard, Dice e Russel & Rao. A adequação dos coeficientes ao conjunto de genótipos foi verificada por correlação entre as matrizes de distância, índice de consenso entre os dendrogramas e otimização de Tocher. As análises mostraram que o coeficiente de Russel e Rao foi divergente em relação aos demais e seu uso não é recomendável. Entre os parâmetros...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Gossypium hirsutum; Diversidade genética; Marcadores RAPD; Marcadores SSR; Genetic diversity; RAPD markers; SSR markers.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122221
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Genetic distances in soybean based on RAPD markers Bragantia
CORRÊA,RONAN XAVIER; ABDELNOOR,RICARDO VILELA; FALEIRO,FÁBIO GELAPE; CRUZ,COSME DAMIÃO; MOREIRA,MAURILIO ALVES; BARROS,EVERALDO GONÇALVES DE.
Four methods were applied to determine pairwise genetic distances among five soybean genotypes which are potential genitors for a mapping population. Additionally, individual plants from the most divergent pair of genotypes were evaluated by the RAPD technique to determine their degree of homozygosity. Genetic distances based on RAPD data were calculated by the modified Rogers' distance, and also by the following arithmetical complements of similarity: simple match, Nei and Li, and Gower. These genetic distances were similar, presenting a correlation coefficient ranging from 0.99 to 1.00. In all four methods lines UFV 91-717 and Ichigowase were the most divergent ones (4.53 to 21.43%). DNA samples from five plants from each of the two most divergent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Genetic diversity; RAPD markers; Estimation methods.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051999000100003
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Genetic diversity analysis of rice cultivars (Oryza sativa L.) differing in salinity tolerance based on RAPD and SSR markers Electron. J. Biotechnol.
Kanawapee,Nantawan; Sanitchon,Jirawat; Srihaban,Pranee; Theerakulpisut,Piyada.
Thirty rice cultivars were evaluated for salinity tolerance during the seedling stage and were divided into five tolerance groups including tolerant (T), moderately tolerant (MT), moderately susceptible (MS), susceptible (S) and highly susceptible (HS) which comprised 5, 10, 9, 4 and 2 cultivars respectively. Genetic diversity of all rice cultivars was evaluated using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers. The cultivars were evaluated for polymorphisms after amplification with 20 random decamer primers and 20 SSR primer pairs. A total of 161 RAPD markers and 190 SSR alleles were produced which revealed 68.94% and 89.47% polymorphism respectively. Mean genetic similarity coefficient was 0.82 for RAPD and 0.70 for...
Tipo: Journal article Palavras-chave: Genetic similarity; RAPD markers; Rice; Salinity tolerance; SSR markers.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600002
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Genetic variability among cashew hybrids and prediction of superior combinations based on agronomic performance PAB
Hawerroth,Maraisa Crestani; Bordallo,Patricia do Nascimento; Oliveira,Luis Cláudio Pessoa; Vale,Egnesio Holanda; Vidal Neto,Francisco das Chagas; Melo,Dheyne Silva.
Abstract: The objective of this work was to evaluate the use of RAPD and ISSR molecular markers to determine the genetic variability among cashew (Anacardium spp.) genotypes, and to indicate possible promising crosses based on cashew genetic variability and phenotypic performance. Ten hybrids - derived from the crosses CCP 76 x BGC 589, CCP 76 x BRS 226, CCP 76 x HAC 276-1, CCP 76 x Embrapa 51, CCP 76 x BRS 253, CCP 76 x HAC222-4, and BRS226 x Embrapa 51 - and their parents were assessed at the molecular level. The hybrids were evaluated for nut yield, mean nut weight, bored nuts, and powdery mildew on nuts (scale 0-4). The RAPD and ISSR markers were efficient in the determinaton of the genetic variability among cashew genotypes, allowing of the grouping...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anacardium; Cashew breeding program; ISSR markers; Nut health; RAPD markers.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102802
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Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers Genet. Mol. Biol.
Rahman,S.M. Zakiur; Khan,Mukhlesur Rahman; Islam,Shahidul; Alam,Samsul.
Genetic variation is a key component for improving a stock through selective breeding programs. Randomly amplified polymorphic DNA (RAPD) markers were used to assess genetic variation in three wild population of the catla carp (Catla catla Hamilton 1822) in the Halda, Jamuna and Padma rivers and one hatchery population in Bangladesh. Five decamer random primers were used to amplify RAPD markers from 30 fish from each population. Thirty of the 55 scorable bands were polymorphic, indicating some degree of genetic variation in all the populations. The proportion of polymorphic loci and gene diversity values reflected a relatively higher level of genetic variation in the Halda population. Sixteen of the 30 polymorphic loci showed a significant (p < 0.05, p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catla catla; Genetic variation; RAPD markers.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100030
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Morphometric and genetic differentiation among populations of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from central Brazil Iheringia, Sér. Zool.
Silva,Daniela de M. e; Cruz,Aparecido D. da; Bastos,Rogério P.; Telles,Mariana P. de C.; Diniz-Filho,José Alexandre F..
To assess genetic structure and phenotypic diversity of Eupemphix nattereri Steindachner, 1863, morphometric and molecular analyses were carried out for nine populations from the State of Goiás. A total of 11 morphometric traits were evaluated and genetic information was estimated using RAPD markers. Genetic and phenotypic distances were determined as a function of geographical origin. Correlation among genetic, morphometric, micro, and macroenviromental were analyzed by the Mantel test. Genetic data indicated high levels of genetic diversity (Φst= 0.3) among the nine populations. Mantel tests did not reveal a significant positive correlation between genetic and geographical distances, indicating that locally geographical populations were not genetically...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eupemphix nattereri; Morphology; RAPD markers; Gene flow; Population structure.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212008000400013
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Population structure analysis of Pimelodus maculatus (Pisces, Siluriformes) from the Tietê and Paranapanema Rivers (Brazil) Genet. Mol. Biol.
Almeida,Fernanda Simões de; Sodré,Leda Maria Koelblinger; Contel,Eucleia Primo Betioli.
Pimelodus maculatus populations from the Tietê and Paranapanema rivers were sampled and had their genetic structure analyzed by using RAPD markers, with the aim of contributing to future conservation studies. The proportion of polymorphic loci was greater than 50% in the populations of both rivers. Genetic diversity data showed that, in spite of its nine hydroelectric plants, the Tietê river population was genetically homogeneous, whereas the Paranapanema river population was structured. This might be due to the presence of high waterfalls distributed all along its course. These data may serve as indicators for future conservation studies on the Tietê and Paranapanema rivers.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD markers; Population structure; Conservation; Genetic variability; Pimelodus.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300014
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Population structure of Eupemphix nattereri (Amphibia, Anura, Leiuperidae) from Central Brazil Genet. Mol. Biol.
Silva,Daniela de Melo e; Cruz,Aparecido Divino da; Bastos,Rogério Pereira; Reis,Raquel Loren; Telles,Mariana Pires de Campos; Diniz-Filho,José Alexandre Felizola.
This study reports on 156 specimens of the amphibian Eupemphix nattereri, a widely distributed leiuperid, obtained from 11 municipalities of central Brazil. The extent of genetic variation was quantified by determining the mean number of alleles per locus and the proportion of polymorphic loci. An analysis of molecular variance (AMOVA) was performed on the random amplified polymorphic DNA (RAPD) haplotypes. The genetic distances obtained by calculating pairwise phist among local samples were used to determine population relationships using the unweighted pair-group method (UPGMA) and non-metric multidimensional scaling (NMDS). The cophenetic correlation was calculated to confirm agreement between the genetic matrix and the unweighted pair group method with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eupemphix nattereri; Gene flow; Population structure; RAPD markers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600022
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Presence of the yeast Candida tropicalis in figs infected by the fruit fly Zaprionus indianus (dip.: Drosophilidae) BJM
Gomes,Luiz H.; Echeverrigaray,Sergio; Conti,José H.; Lourenço,Marcus Vinicius M.; Duarte,Keila M.R..
In the last years, the fruit fly Zapronius indianus became the most important plague of Brazilian fig production. A fermentation process is associated with infection of the fruit by this fly. A single yeast species, Candida tropicalis, was identified in all the infected figs. The presence of one species and the low genetic variability (RAPD) of the isolates indicates an uncommonly strict interaction between C. tropicalis and Z. indianus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zaprionus indianus; Candida tropicalis; RAPD markers; Yeast-insect association.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100002
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Proximal causes of genetic variation between and within populations of rauli (Nothofagus nervosa) Ciencia e Investigación Agraria
Carrasco,Basilio; Garnier,Maurice; Eaton,Lafayette; Guevara,Rafael; Carú,Margarita.
Random Amplified Polymorphic DNA(RAPD)markers were used to assess the genetic diversity of 587 individuals, belonging to 22 populations of Nothofagus nervosa that were distributed through the Coastal (38°S to 41 °S) and Andes Mountains in Central-Southern Chile (36°S to 40°S). The objective of this study was to complement the genetic inferences previously determined by isozyme analysis, in order to obtain more accurate genetic diversity estimations. We scored 81.8% of the polymorphic loci of the samples tested. The average incidence of genetic polymorphism within populations was high, with values ranging between 33% and 63%. Analysis of molecular variance (AMOVA) showed most of the genetic variation was distributed within populations (87.6%), but FST...
Tipo: Journal article Palavras-chave: Genetic structure; Nothofagus; RAPD markers.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000200007
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Selection of common bean segregating populations using genetic and phenotypic parameters and RAPD markers. Repositório Alice
MENDONÇA, H. A.; SANTOS, J. B. dos; RAMALHO, M. A. P..
In an attempt to identify strategies for choosing common bean segregating populations, the diallel parameters, the contribution of homozygous (m+a?) and heterozygous (d) loci, the heritability (h2a), the genetic distance (RAPD), and the selection potential (Z) were estimated in a study carried out in two stages. In the first, 30 hybrid combinations (partial diallel) were assessed in the F2 and F3 generations. Increasing grain yield was due dominance effect (d) although, both GCA and SCA were significant. d varied greatly and gave an information like SCA, however h2a alone was not indicative of success in a breeding program. In the second stage, five populations with lesser d and five with higher were selected, and twenty-seven families were taken from...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Parâmetro fenotípico; Marcador RAPD; RAPD markers; Melhoramento genético vegetal; Feijão; Phaseolus vulgaris; Parâmetro genético; Marcador molecular; Plant breeding; Beans; Genetic markers; Fitomejoramiento; Frijoles; Marcadores genéticos.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/508871
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Somaclonal variation in Cereus peruvianus Mill. (Cactaceae): its potential to generate new varieties and broaden the species's genetic basis JBAG
Resende Gazoli,Adriana; Mangolin,Claudete Aparecida; Machado,Maria de Fátima.
In current study Random Amplified DNA-Polymorphism markers were used to detect genetic variations in C. peruvianus somaclones and to examine relationships among somaclones and plants grown from seeds in cultivated populations. The possible association of DNA fragments with morphological traits of somaclones was also investigated. Polymorphism in somaclones (62.24%) was higher than that detected in cultivated plants (42.57%). The casual grouping of somaclones presented atypical and typical shoot phenotypes showed that the random amplifi ed DNA-fragment polymorphisms detected in present study were not associated with shoot morphologies. Similarity in somaclones and cultivated plants ranged from 70.2 to 93.7% and 76.7 to 86.8%, respectively. Thus, in vitro...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cactus; Genetic diversity; Mandacaru; RAPD markers; Somaclonal variation.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332010000100004
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การประเมินความสัมพันธ์ทางพันธุกรรมของพลับในประเทศไทยด้วยเครื่องหมาย RAPD Thai Agricultural
Kriengsak Thaipong.
Special Problem (M.Sc. in Agriculture)
Tipo: PhysicalObject Palavras-chave: Persimmon; Diospyros kaki; Diospyros lotus; Diospyros glandulosa; Genetics; Genetics relationships; Cultivar identification; RAPD markers; Random amplified polymorphic dna; Evaluation; Thailand; พลับ; สายพันธุ์; พันธุกรรม; ความสัมพันธ์ทางพันธุกรรม; การจำแนกพันธุ์; เครื่องหมายทางพันธุกรรม; เทคนิคสุ่มขยายปริมาณชิ้นดีเอ็นเอ; ประเทศไทย.
Ano: 2002 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5495
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