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A new species of bird’s nest fungi: characterisation of Cyathus subglobisporus sp. nov. based on morphological and molecular data Naturalis
Zhao, R.-L.; Desjardin, D.E.; Soytong, K.; Hyde, K.D..
Recent collections of bird’s nest fungi (i.e. Crucibulum, Cyathus, Mycocalia, Nidula, and Nidularia species) in northern Thailand resulted in the discovery of a new species of Cyathus, herein described as C. subglobisporus. This species is distinct by a combination of ivory-coloured fruiting bodies covered with shaggy hairs, plications on the inner surface of the peridium and subglobose basidiospores. Phylogenetic analyses based on ITS and LSU ribosomal DNA sequences using neighbour-joining, maximum likelihood and weighted maximum parsimony support Cyathus subglobisporus as a distinct species and sister to a clade containing C. annulatus, C. renweii and C. stercoreus in the Striatum group.
Tipo: Article / Letter to the editor Palavras-chave: Bird’s nest fungi; Gasteromycetes; New species; Phylogeny; RDNA.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/532073
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Análise molecular do rDNA de Hemileia vastatrix. Repositório Alice
SANTANA, M. F.; ZAMBOLIM, E. M.; OLIVEIRA, L. O. de; CAIXETA, E. T.; ZAMBOLIM, L..
Os espaçadores transcritos internos (ITS1 e ITS2) e o gene 5,8S do rDNA nuclear de 15 populações de H. vastatrix foram amplificados por PCR com o objetivo de estudar a diversidade genética. Os fragmentos amplificados foram clonados, e dos clones resultantes, 4 a 7 foram seqüenciados. As 82 seqüências resultantes revelaram a existência de 68 haplótipos definidos por 63 substituições de base e cinco indels. Entre os 68 haplótipos, 64 foram exclusivos, isto é, cada um deles foi detectado em uma única lavoura, dois (1 e 2) foram compartilhados entre lavouras distintas e dois (19 e 29) foram exclusivos, mas detectados duas vezes na mesma lavoura. Os haplótipos geraram uma rede única mostrando que 65 deles são de origem mais recente e de distribuição restrita, e...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Hemileia vastatrix; Haplótipos; ITS (Internal transcribed spacer); RDNA; Diversidade genética.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/906053
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Caracterização de bactérias endofíticas isoladas do milho cultivado no agroecossistema cerrado. Repositório Alice
COELHO, V. T. S.; QUINTÃO, P. L.; PAOLI, H. C.; MELO, M. A. V.; LAGE, G. A.; FONSECA, P. C.; BRESSAN, W.; GUIMARAES, C. T.; GOMES, E. A.; FIGUEIREDO, J. E. F..
2002
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: SDS-PAGE; RAPD-PCR; RDNA; Sequenciamento; Taxinomia.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485688
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Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species Electron. J. Biotechnol.
Tosto,Daniela; Hopp,Esteban.
Oxalis tuberosa is an octoploid Andean tuber crop called "oca" that belongs to the worldwide distributed genus Oxalis. The genus is very heterogeneous and its systematics is still problematic. It has been proposed that O. tuberosa evolved by polyploidization of a still not defined ancestor that belongs to an alliance of species sharing the same basic chromosome number (x = 8). Nuclear rDNA units of O. tuberosa and a selected group of four related diploid species were characterised by RFLP using different restriction endonucleases and southern hybridization probes to produce a restriction map for EcoRI and BamHI. The major rDNA unit length in O. tuberosa was estimated at 10.7 kbp. As expected, restriction site variation was observed mainly in the intergenic...
Tipo: Journal article Palavras-chave: Andean crops; Oca; Oxalis; RDNA; RFLPs.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300002
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Chromatin differentiation between Vigna radiata (L.) R. Wilczek and V. unguiculata (L.) Walp. (Fabaceae). Repositório Alice
BORTOLETI, K. C. de A.; ISEPPON, A. M. B.; MELO, N. F. de; VIDAL, A. C. B..
2012
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: RDNA; CMA3/DAPI; Heterocromatina; Feijão caupi; Vigna unguiculata; Vigna radiata; Feijão.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/919299
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Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae) Neotropical Ichthyology
Marques,Debora Karla; Venere,Paulo Cesar; Galetti Junior,Pedro Manoel.
The mitotic chromosomes of the pirarucu Arapaima gigas inhabiting the middle Araguaia River and collected in the municipality of Araguaiana (MT, Brazil) were studied. The chromosomes were analyzed through Giemsa staining, C-banding, Ag-NOR staining and in situ hybridization using an 18S rRNA gene probe. The karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. No cytologically distinguishable sex chromosome was identified. A single NOR-bearing chromosome pair was detected by Ag-NOR staining and confirmed by 18S rDNA- FISH. Faint constitutive heterochromatin was C-banded in the centromeric region of some chromosomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pirarucu; Karyotype; RDNA; Heterochromatin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252006000200007
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Chromosome mapping of ribosomal genes and histone H4 in the genus Radacridium (Romaleidae) Genet. Mol. Biol.
Anjos,Allison; Loreto,Vilma; Souza,Maria José de.
In this study, two species of Romaleidae grasshoppers, Radacridium mariajoseae and R.nordestinum, were analyzed after CMA3/DA/DAPI sequential staining and fluorescence in situ hybridization (FISH) to determine the location of the 18S and 5S rDNA and histone H4 genes. Both species presented karyotypes composed of 2n = 23, X0 with exclusively acrocentric chromosomes. CMA3+ blocks were detected after CMA3/DA/DAPI staining in only one medium size autosome bivalent and in the X chromosome in R. mariajoseae. On the other hand, all chromosomes, except the L1 bivalent, of R. nordestinum presented CMA3+ blocks. FISH analysis showed that the 18S genes are restricted to the X chromosome in R. mariajoseae, whereas these genes were located in the L2,S9 and S10...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; FISH; Fluorochromes; Grasshoppers; RDNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300006
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Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences BABT
Lopez,Ana Maria Queijeiro; Lucas,John Alexander.
Thirty six isolates of fungi obtained from anthracnose lesions of cashew and associated host plants in Brazil, were compared by their cultural, morphological and partial sequences of the 28S ribosomal DNA characters. They showed a high degree of cultural variability. The average mycelial growth rate on all tested media ranged from 10.2-13.3 mm/day between the isolates. Most of them produced perithecia (sterile and fertile) and some produced setae (sterile and fertile). All the isolates produced acervuli with predominantly cylindrical conidia (12.4-17.7 µmX 4.8-6.0 µm in width) with round ends, which became septate on germination, and produced unlobed or slightlylobed appressoria. Comparison of the D2 domain of the large subunit (LSU) rDNA sequences with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracnose; Inflorescence blight; Colletotrichum; RDNA; Cashew; Anacardium occidentale.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400001
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Cytogenetic analyses of two endemic fish species from the São Francisco River basin: Conorhynchus conirostris and Lophiosilurus alexandri (Siluriformes) Genet. Mol. Biol.
Marques,Marilza Barbosa de Almeida; Moreira-Filho,Orlando; Garcia,Caroline; Margarido,Vladimir Pavan.
Two Siluriformes species endemic to the São Francisco River basin were characterized by conventional and differential cytogenetic analyses involving C-banding, Ag-nucleolar organizer region (NOR) and chromomycin A3 (CMA3) staining, and FISH (fluorescent in situ hybridization) with 18S and 5S rDNA probes. Conorhynchus conirostris presents a higher diploid number (2n = 60) than those detected in Pimelodidae representatives, whereas Lophiosilurus alexandri, with a karyotype of 2n = 54 chromosomes, presents a chromosomal constitution similar to that found in the family Pseudopimelodidae. Plesiomorphic characteristics such as single NORs at terminal positions are found in both species, as revealed by CMA3 and silver nitrate staining, and FISH with a 18S rDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Incertae sedis; Pimelodidae; Pseudopimelodidae; RDNA; Siluriformes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200008
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Cytogenetic analysis of Astyanax laticeps (Cope, 1894) (Ostariophysi: Characidae) from the laguna dos Patos system Neotropical Ichthyology
Rosa,Renata da; Rubert,Marceléia; Malabarba,Luiz R.; Martins-Santos,Isabel Cristina; Giuliano-Caetano,Lucia.
The genus Astyanax is prominent among Characiformes, due to the large number of species found and its wide geographic distribution. In this work, Astyanax laticeps specimens from the laguna dos Patos system were cytogenetically analyzed. A diploid number of 2n = 50 chromosomes distributed into 6m+16sm+16st+12a (FN = 88) was found, without differences between males and females. A few small heterochromatin blocks were observed, besides three more conspicuous C-bands, corresponding to NORs, as confirmed by silver nitrate and CMA3 staining, FISH, and DAPI negative staining. These regions were located in a medium-sized subtelocentric and in a large subtelocentric chromosomal pair, probably because of a deletion of this region in one homologous chromosome, or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax scabripinnis species complex; RDNA; Homologous transposition.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400007
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Cytogenetic analysis of Baryancistrus xanthellus (Siluriformes: Loricariidae: Ancistrini), an ornamental fish endemic to the Xingu River, Brazil Neotropical Ichthyology
Medeiros,Larissa A.; Ginani,Eduardo G.; Sousa,Leandro M.; Py-Daniel,Lúcia H. Rapp; Feldberg,Eliana.
ABSTRACT Baryancistrus xanthellus is a species from the Ancistrini tribe known commonly as "amarelinho " or "golden nugget pleco". It is one of the most popular and valued ornamental fishes due to its color pattern. Also, it is an endemic species from the Xingu River occurring from Volta Grande do Xingu, region where the Belo Monte Hydropower Dam is being built, to São Félix do Xingu. The current study aimed to cytogenetically characterize B. xanthellus . Results point to the maintenance of 2n=52, which is considered the most common condition for the tribe, and a single nucleolus organizer region (NOR). Mapping of the 18S rDNA confirmed the NOR sites, and the 5S rDNA was mapped in the interstitial position of a single chromosome pair. The 18S and 5S rDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FISH; Habitat endangered; RDNA; Volta Grande do Xingu.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200212
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Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil Genet. Mol. Biol.
Nogueira,Lídia; Zanoni,Juliani Bruna; Solé,Mirco; Affonso,Paulo Roberto Antunes de Mello; Siqueira,Sérgio; Sampaio,Iracilda.
Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amphibians; Chromosomes; Fluorochromes; Heterochromatin; RDNA.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156
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Cytogenetic variation of repetitive DNA elements in Hoplias malabaricus (Characiformes - Erythrinidae) from white, black and clear water rivers of the Amazon basin Genet. Mol. Biol.
Santos,Fabíola Araújo dos; Marques,Diego Ferreira; Terencio,Maria Leandra; Feldberg,Eliana; Rodrigues,Luís Reginaldo R..
Abstract Hoplias malabaricus is a common fish species occurring in white, black and clear water rivers of the Amazon basin. Its large distribution across distinct aquatic environments can pose stressful conditions for dispersal and creates possibilities for the emergence of local adaptive profiles. We investigated the chromosomal localization of repetitive DNA markers (constitutive heterochromatin, rDNA and the transposable element REX-3) in populations from the Amazonas river (white water), the Negro river (black water) and the Tapajós river (clear water), in order to address the variation/association of cytogenomic features and environmental conditions. We found a conserved karyotypic macrostructure with a diploid number of 40 chromosomes (20...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenomics; FISH; RDNA; REX 3; Tapajós river.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100040
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Determinação da seqüência parcial de nucleotídeos do gene do RNA ribossômico 18S de caprinos (Capra hiscus). Repositório Alice
SOUZA, R. B.; ARRUDA, F. V. S.; PINHEIRO, R. R.; CUNHA, R. M. S. da.
2005
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Caprino; Genética animal; Melhoramento genético animal; RDNA; Viabilidade genética.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/handle/doc/533424
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Diferenciação de biótipos de Bemísia tabaci utilizando PCR-RFLP e sequenciamento da região ITS1 rDNA. Infoteca-e
RABELLO, A. R.; QUEIROZ, P. R.; SIMÕES, K. C.; HIRAGI, C. O.; LIMA, L. H. C.; OLIVEIRA, M. R. V.; MEHTA, A..
2005
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Bemísia tabaci; Mosca branca; Biótipos; PCR-RFLP; ITS1; RDNA; Bemisia; Biotype.
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/187042
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Differential transcription of ribosomal cistrons denoting nucleolar dominance in hybrids of Drosophila mulleri and Drosophila navojoa (mulleri complex, Repleta group) Genet. Mol. Biol.
Commar,Leliane Silva; Bicudo,Hermione E.M.C.; Rahal,Paula; Ceron,Carlos Roberto.
The fruit flies Drosophila mulleri and Drosophila navojoa are included in the mulleri complex of the mulleri subgroup and Repleta group. Although there is no demonstration that interspecific crosses between them occur in nature, they intercross in the laboratory in both cross directions. Previous data have shown the occurrence of nucleolar dominance in interspecific hybrids of some species in the mulleri complex. We investigated nucleolar dominance in D. mulleri/D. navojoa hybrids using the transcription profiles of the rDNA internal transcribed spacer (ITS-1) region. The results showed that the ribosomal cistrons present in the X chromosome and in the microchromosome of D. navojoa are exclusively or preferentially transcribed in these hybrids depending on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila mulleri; D. navojoa; Interspecific hybrids; Nucleolar dominance; RDNA.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600028
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Diversidade genética de Colletotrichum graminicola isolados da cultura do sorgo no ecossistema agrícola tropical revelada por SDS-PAGE, RAPD e análise da sequência do RDNA 18s e região intergênica 18s-28s Repositório Alice
PAOLI, H.C.; QUINTAO, P.L.; COELHO, V.T.S.; LAGE, G.A.; FONSECA, P.C.; FERREIRA, A.S.; CASELA, C.R.; GUIMARAES, C. T.; GOMES, E.A.; FIGUEIREDO, J. E. F..
2002
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: RDNA; RAPD-PCR; ARDRA; Sequenciamento; SDS-PAGE.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485693
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Efficiency of IRAP and ITS-RFLP marker systems in accessing genetic variation of Pyrenophora graminea Genet. Mol. Biol.
Zein,Imad; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin.
The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% polymorphic bands, respectively, with a corresponding mean polymorphic information content (PIC) of 0.38 and 0.36. The IRAP marker index ratio (2.41) was higher than ITS-RFLP (1.50). On one hand, the quality nature of data (QND) was higher for ITS-RFLP (0.169) than IRAP (0.093). However, correlation between both marker similarity matrices was significant (r = 0.34, p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyrenophora graminea; IRAP; Internal transcribed spacer; RDNA.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200021
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential. Repositório Alice
MAIA, N. da C.; SOUZA, P. N. da C.; GODINHO, B. T. V.; MOREIRA, S. I.; ABREU, L. M. de; JANK, L.; CARDOSO, P. G..
This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Antagonism; Molecular identification; RDNA; Grasses; Plant pathogens.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110371
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential R. Bras. Zootec.
Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303
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