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Costa, S.C.P.; Ribeiro, C.; Girard, P.A.; Zumbihl, R.; Brehélin, M.. |
Haemocytes are the main immunocompetent cells in insect cellular immune reactions. Here, we show that in Spodoptera littoralis, granular haemocytes are the primary phagocyte haemocytes, both in vivo and in vitro. The "trigger" and "zipper" modes of engulfment known in mammal macrophages are active, in vivo, in S. littoralis granular haemocytes, together with macropinocytosis. Lipopolysaccharide as well as lipoteichoic acid inhibit the binding of both Gram-positive (Corynebacterium xerosis) and Gramnegative (Escherichia coli) bacteria on granular haemocytes. In addition, different ligands can inhibit the binding of E coli. Most of these inhibitors are known as ligands of scavenger receptors in mammal macrophages and we hypothesise that one of the receptors... |
Tipo: Journal Article |
Palavras-chave: SPODOPTERA LITTORALIS; NOCTUELLE MACROPINOCYTOSIS; TRIGGER; ZIPPER; RECEPTOR; INSECT. |
Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201069bfa10d&uri=/notices/prodinra1/2011/05/ |
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Ramoni, R.; Vincent, F.; Grolli, S.; Conti, V.; Malosse, C.; Boyer, F.D.; Nagnan-Le Meillour, P.; Spinelli, S.; Cambillau, C.; Tegoni, M.. |
Bovine odorant-binding protein (bOBP) is a dimeric lipocalin present in large amounts in the respiratory and olfactory nasal mucosa. The structure of bOBP refined at 2.0-Å resolution revealed an elongated volume of electron density inside each buried cavity, indicating the presence of one (or several) naturally occurring copurified ligand(s) (Tegoni et al. (1996)Nat. Struct. Biol. 3, 863–867; Bianchet et al.(1996) Nat. Struct. Biol. 3, 934–939). In the present work, by combining mass spectrometry, x-ray crystallography (1.8-Å resolution), and fluorescence, it has been unambiguously established that natural bOBP contains the racemic form of 1-octen-3-ol. This volatile substance is a typical component of bovine breath and in general of odorous body... |
Tipo: Journal Article |
Palavras-chave: TECHNIQUE ANALYTIQUE; SPECTROMÉTRIE DE MASSE; CHROMATOGRAPHIE EN PHASE GAZEUSE; STRUCTURE TRIDIMENSIONNELLE; LIGAND FLUORESCENCE SPECTROSCOPY; URINARY PROTEINS; NASAL-MUCOSA; AFFINITIES; MECHANISM; LIPOCALIN; MOSQUITOS; RECEPTOR. |
Ano: 2001 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200012319094103&uri=/notices/prodinra1/2010/10/ |
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