Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 22
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo BJMBR
Wang,X.M.; Gao,S.J.; Guo,X.F.; Sun,W.J.; Yan,Z.Q.; Wang,W.X.; Xu,Y.Q.; Lu,D..
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CIAPIN1 gene; Multidrug resistance; RNA interference; MDR1 gene; Breast neoplasms.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400273
Imagem não selecionada

Imprime registro no formato completo
Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells BJMBR
Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L..
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548
Imagem não selecionada

Imprime registro no formato completo
Fruit fly management research, transcriptome analysis and first evidence of RNAi in Anastrepha fraterculus (Diptera: Tephritidae). Repositório Alice
DIAS, N. P..
bitstream/item/211497/1/Dori-tese-Nayma-revisada.pdf
Tipo: Tese/dissertação (ALICE) Palavras-chave: RNA-Seq; RNAi-functional; South American fruit fly; Systematic review; RNA interference.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120911
Imagem não selecionada

Imprime registro no formato completo
Gene silencing and mortality in larval stages of Anastrepha fraterculus induced by RNAi. Repositório Alice
DIAS, N. P.; CAGLIARI, D.; SMAGGHE, G.; NAVA, D. E.; ZOTTI, M. J..
bitstream/item/211314/1/Dori1-Anais-Siconbiol-2019.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: South American fruit fly; RNA interference; DsRNA viruses.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1120675
Imagem não selecionada

Imprime registro no formato completo
In vitro and in vivo inhibition of rabies virus replication by RNA interference BJM
Ono,Ekaterina A. Durymanova; Iamamoto,Keila; Castilho,Juliana G.; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Achkar,Samira M.; Carrieri,Maria L.; Kotait,Ivanete; Brandão,Paulo E..
Rabies is a zoonotic disease that affects all mammals and leads to more than 55,000 human deaths every year, caused by rabies virus (RABV) (Mononegavirales: Rhabdoviridae: Lyssavirus). Currently, human rabies treatment is based on the Milwaukee Protocol which consists on the induction of coma and massive antiviral therapy. The aim of this study was to assess the decrease in the titer of rabies virus both in vitro and in vivo using short-interfering RNAs. To this end, three siRNAs were used with antisense strands complementary to rabies virus nucleoprotein (N) mRNA. BHK-21 cells monolayers were infected with 1000 to 0.1 TCID50 of PV and after 2 hours the cells were transfected with each of tree RNAs in separate using Lipofectamine-2000. All three siRNAs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; RNA interference; SiRNAs; Antiviral; Treatment.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300034
Imagem não selecionada

Imprime registro no formato completo
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas ArchiMer
Huvet, Arnaud; Fleury, Elodie; Corporeau, Charlotte; Quillien, Virgile; Daniel, Jean-yves; Riviere, Guillaume; Boudry, Pierre; Fabioux, Caroline.
We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated...
Tipo: Text Palavras-chave: Marine bivalve; Crassostrea gigas; Reproduction; RNA interference; Transforming growth factor-beta.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19794/17486.pdf
Imagem não selecionada

Imprime registro no formato completo
Increase in the efficacy of gene silencing using viroid-like dsRNAs in the insect pest cotton boll weevil. Repositório Alice
GARCIA, R. A.; MACEDO, L. L. P.; SA, M. F. G. de.
bitstream/item/190915/1/GROSSI-2.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Insect controle; Viroid structure; RNA interference.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104490
Imagem não selecionada

Imprime registro no formato completo
Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis Genet. Mol. Biol.
Chiang,Hsin-I; Berghman,Luc R.; Zhou,Huaijun.
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA interference; NF-kBp50; Macrophage; Salmonella; Chicken.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013
Imagem não selecionada

Imprime registro no formato completo
Inhibition of STAT3 by RNA interference suppresses angiogenesis in colorectal carcinoma BJMBR
Qian,W.F.; Guan,W.X.; Gao,Y.; Tan,J.F.; Qiao,Z.M.; Huang,H.; Xia,C.L..
In order to investigate signal transduction and activation of transcription 3 (STAT3) signaling on angiogenesis in colorectal carcinoma (CRC) after inhibiting STAT3 expression, we constructed the HT-29-shSTAT3 cell line by lentivirus-mediated RNAi. Cell growth was assessed with MTT and the cell cycle distribution by flow cytometry. CRC nude mouse models were established and tumor growth was monitored periodically. On day 30, all mice were killed and tumor tissues were removed. Microvessel density (MVD) was determined according to CD34-positive staining. The expression of vascular endothelial growth factor A (VEGFA), matrix metalloproteinase-2 (MMP2) and basic fibroblast growth factor (FGF2) was monitored by quantitative real-time PCR and Western blot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: STAT3; RNA interference; Colorectal carcinoma; Angiogenesis; VEGFA; MMP2; FGF2.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200005
Imagem não selecionada

Imprime registro no formato completo
Insights into the antiviral functions of the RNAi machinery in penaeid shrimp ArchiMer
Labreuche, Yannick; Warr, Gregory W..
Over the last decade, RNA interference pathways have emerged in eukaryotes as critical regulators of many diverse biological functions including, among others, transcriptional gene regulation, post-transcriptional gene silencing, heterochromatin remodelling, suppression of transposon activity, and antiviral defences. Although this gene silencing process has been reported to be relatively well conserved in species of different phyla, there are important discrepancies between plants, invertebrates and mammals. In penaeid shrimp, the existence of an intact and functional RNAi machinery is supported by a rapidly growing body of evidence. However, the extent to which this process participates to the host immune responses remains poorly defined in this non-model...
Tipo: Text Palavras-chave: Shrimp; Innate immune response; In vertebrate immunity; RNA interference; Virus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00119/23063/20899.pdf
Imagem não selecionada

Imprime registro no formato completo
Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer Biol. Res.
Zhao,Xiaolan; Chen,Man; Tan,Jishan.
BACKGROUND: Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS: Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluate ZFR gene expression in human pancreatic tumors. Then short hairpin RNA (shRNA) targeting ZFR was designed and delivered into PANC-1 pancreatic cancer cells to knock down ZFR expression. Cell viability, cell proliferation and cell cycle analysis after ZFR knockdown were determined by MTT, colony forming and FACS, respectively. In addition, cell migration and invasion were assessed using the Transwell system. RESULTS: The expression of ZFR was significantly...
Tipo: Journal article Palavras-chave: Pancreatic cancer; RNA interference; Targeted therapy; Zinc finger RNA binding protein.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100026
Imagem não selecionada

Imprime registro no formato completo
Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp ArchiMer
Labreuche, Yannick; O'Leary, Nuala A.; De La Vega, Enrique; Veloso, Artur; Gross, Paul S.; Chapman, Robert W.; Browdy, Craig L.; Warr, Gregory W..
injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection...
Tipo: Text Palavras-chave: Shrimp; Virus; RNA interference; Invertebrate immunity; Innate immune response; Toll like receptor; DSRNA.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6428.pdf
Imagem não selecionada

Imprime registro no formato completo
Lentiviral-mediated RNAi targeting caspase-3 inhibits apoptosis induced by serum deprivation in rat endplate chondrocytes in vitro BJMBR
Ding,L.; Wu,J.P.; Xu,G.; Zhu,B.; Zeng,Q.M.; Li,D.F.; Lu,W..
Current studies find that degenerated cartilage endplates (CEP) of vertebrae, with fewer diffusion areas, decrease nutrient supply and accelerate intervertebral disc degeneration. Many more apoptotic cells have been identified in degenerated than in normal endplates, and may be responsible for the degenerated grade. Previous findings suggest that inhibition of apoptosis is one possible approach to improve disc regeneration. It is postulated that inhibition of CEP cell apoptosis may be responsible for the regeneration of endplates. Caspase-3, involved in the execution phase of apoptosis, is a candidate for regulating the apoptotic process. In the present study, CEP cells were incubated in 1% fetal bovine serum. Activated caspases were detected to identify...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cartilaginous endplate; Chondrocytes; RNA interference; Apoptosis; Caspase-3; Serum deprivation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600445
Imagem não selecionada

Imprime registro no formato completo
Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication ArchiMer
Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters...
Tipo: Text Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf
Imagem não selecionada

Imprime registro no formato completo
Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1 Genet. Mol. Biol.
Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin.
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prostate cancer; DNA methylation; DNMT3; RNA interference.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024
Imagem não selecionada

Imprime registro no formato completo
Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase ArchiMer
Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf
Imagem não selecionada

Imprime registro no formato completo
Review: potential biotechnological assets related to plant immunity modulation applicable in engineering disease-resistant crops. Repositório Alice
SILVA, M. S.; ARRAES, F. B. M.; CAMPOS, M. de A.; GROSSI-DE-SA, M.; FERNANDEZ, D.; CÂNDIDO, E. de S.; CARDOSO, M. H.; FRANCO, O. L.; GROSSI-DE-SA, M. F..
bitstream/item/177697/1/1-s2.0-S0168945217309421-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pathogen-associated molecular pattern-triggered immunity (PTI); Effector-triggered susceptibility (ETS); Effector-triggered immunity (ETI); Genetically modified (GM) crop; RNA interference; Disease resistance.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1091968
Imagem não selecionada

Imprime registro no formato completo
RNAi-mediated silencing of the Bmi-1 gene causes growth inhibition and enhances doxorubicin-induced apoptosis in MCF-7 cells Genet. Mol. Biol.
Wu,Xiang-mei; Liu,Xing; Bu,You-quan; Sengupta,Joyeeta; Cui,Hong-juan; Yi,Fa-ping; Liu,Tao; Yuan,Chen-fu; Shi,Yan-yan; Song,Fang-zhou.
The oncogene Bmi-1 is a member of the Polycomb group gene family. Its expression is found to be greatly increased in a number of malignant tumors including breast cancer. This could suggest Bmi-1 as a potent therapeutic target. In this study, RNAi was introduced to down-regulate the expression of Bmi-1 in a highly malignant breast adenocarcinoma cell line, MCF-7. A thorough study of the biological behavior and chemosensitivity changes of the MCF-7 cells was carried out in context to the therapeutic potential of Bmi-1. The results obtained indicated that siRNA targeting of Bmi-1 could lead to an efficient and specific inhibition of endogenous Bmi-1 activity. The mRNA and protein expression of Bmi-1 were determined by RT-PCR and Western blot, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA interference; Bmi-1; Retrovirus vector; Doxorubicin; Breast cancer.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400004
Imagem não selecionada

Imprime registro no formato completo
Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA BJM
Ono,Ekaterina Alexandrovna Durymanova; Taniwaki,Sueli Akemi; Brandão,Paulo.
Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; Treatment; RNA interference.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300566
Imagem não selecionada

Imprime registro no formato completo
Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment ArchiMer
Beguel, Jean-philippe; Huvet, Arnaud; Quillien, Virgile; Lambert, Christophe; Fabioux, Caroline.
Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted...
Tipo: Text Palavras-chave: Antioxidant capacity; Crassostrea gigas; Reproduction; RNA interference; ROS.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00116/22744/21494.pdf
Registros recuperados: 22
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional