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| Registros recuperados: 65 | |
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| OLIVEIRA, J. C. de; SILVA, C. C. da; SOUZA, A. P. de; CAMPOS, T. de. |
| The development of specific protocols for extraction of high-quality RNA are key advances in molecular techniques for elucidating biological processes and mechanisms. The forage peanut has significant economic value for use in mixed pastures. However, it is difficult to extract high-quality RNA from forage peanuts due to high contents of tannins, polysaccharides, and other secondary metabolites. Here, it is described an efficient method for obtaining high-quality, high-yield total RNA that is usable in downstream transcriptome analysis. This modifications helped eliminate the problems associated with the presence of high concentrations of secondary metabolites in forage peanut leaves, and lacked signs of degradation. O desenvolvimento de protocolos... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Amendoim forrageiro; Forage peanut; Cacahuetes forrajeros; Ácido ribonucleico; Metabólico secundário; Secondary metabolic; High quality; Alta calidad; RNA extraction; Extracción de ARN; Leguminosa Forrageira; Melhoramento Genético Vegetal; RNA; Extração; Rendimento; Qualidade; Forage legumes; Arachis pintoi; Plant breeding. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125058 |
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| PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P.. |
| An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN ≥ 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes β-tubulin and T197 were ranked as the most stable among the sample sets... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Gene normalization; Molecular breeding; Grain RNA extraction; Feijão; Phaseolus vulgaris; Melhoramento; RNA; Beans; Molecular breeding; Gene expression. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076282 |
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| SOUZA, J. R. T; IBELLI, A. M. G.; REGITANO, L. C. de A.. |
| Amostras de RNA com elevado grau de pureza e integridade são necessárias em diversos métodos de análise da expressão gênica. Em virtude da possibilidade de degradação enzimática, métodos eficazes de extração e armazenamento dessas amostras são necessários. Existem hoje no mercado reagentes que permitem a extração e a purificação de RNA de diversas amostras, com rapidez e eficiência, pois contêm simultaneamente desnaturantes, como o isotiocianato de guanidina, que impedem a ação enzimática, e solventes orgânicos que isolam o RNA do DNA e das proteínas. O objetivo deste projeto foi comparar a qualidade e integridade de RNA extraídos a partir de tecidos armazenados em RNAlater. e de tecidos crioliofilizados, e testar diferentes métodos de armazenamento para... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: RNA; Métodos de conservação; Crioliofilização. |
| Ano: 2007 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/48100 |
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| VITOR, A. B.; COSTA NETO, B. P. da; FERREIRA, J. D.; MARTINS, D. R. P. S.; CHAVES, A. R. de M.; AIDAR, S. de T.; MORGANTE, C. V.. |
| Este trabalho foi desenvolvido com o objetivo de avaliar a resposta fisiológica de dois acessos de Manihot esculenta submetidos ao estresse hídrico, em plantas com 4 e 2 meses, e estabelecer um protocolo para a extração de RNA total de folhas e raízes. Foi realizado um experimento em casa de vegetação com 96 plantas dos acessos Cacau e 2020 do Banco de Germoplasma da Embrapa Mandioca e Fruticultura, distribuídas em três blocos casualizados. Foram medidos parâmetros de crescimento como expansão foliar, diâmetro do caule, altura da planta e a massa seca de folhas, caules, raízes e raízes comerciais. Foram também realizadas medidas do teor e fluorescência da clorofila. Observou-se que, no geral, o comportamento em resposta ao estresse hídrico é semelhante... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Estresse hídrico; RNA; Ácido nucleico; Acesso; Mandioca; Melhoramento genético; Manihot esculenta. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1013003 |
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| SILVA, J. B. da; SILVA, R. L. de O.; FERREIRA NETO, J. R. C.; ARAÚJO, F. T. de; PANDOLFI, V.; ARAÚJO, A. C. C. de; LEMOS, A. B.; RÊGO, M. de S.; OLIVEIRA, M. F. de; MELO, N. F. de; ISEPPON, A. M. B.. |
| Este trabalho objetivou testar diferentes protocolos de extração de RNA para tecidos foliares de videira infectadas por X. campestres e identificar o mais eficiente para o tecido avaliado.. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Vitis vinifera; Xanthomonas campestres; RNA; Uva; Melhoramento genético; Folha; Doença; Bactéria; Genetic improvement. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1057616 |
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| DRESCH,DAIANE M.; MASETTO,TATHIANA E.; SCALON,SILVANA P.Q.. |
| The aim of this study was to evaluate the sensitivity of Campomanesia adamantium seeds to desiccation by drying in activated silica gel (fast) and under laboratory conditions (slow). To assess the sensitivity of the seeds to desiccation, we used drying with silica gel and drying under laboratory conditions (25 °C), in order to obtain seeds with moisture content of 45, 35, 30, 25, 20, 15, 10 and 5%. The physiological potential of the seeds after desiccation was evaluated by measuring primary root protrusion, percentage of normal seedlings, germination seed index, seedling length, total seedling dry mass, electrical conductivity and DNA and RNA integrities. The C. adamantium seeds were sensitive to desiccation and to a reduction in moisture content to 21.1%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; DNA; Drying; RNA; Viability. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000502217 |
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| García-Nogales,Paula; Serrano,Alicia; Secchi,Sonia; Gutiérrez,Serafín; Arís,Anna. |
| Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isolation of bacterial RNA from spiked UHT milk samples. The kits were compared in terms of extraction efficiency and RNA purity using two bacterial species, the Gram negative Escherichia coli and the Gram positive Staphylococcus aureus. Two kits are based in silica-matrix extraction, and the other two in the guanidinium thiocyanate-phenol-chloroform extraction. In our... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Milk; Purification; RNA. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500019 |
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| VILLELA, L. C. V.; ALVES, A. L.; VARELA, E. S.; YAMAGISHI, M. E. B.; GIACHETTO, P. F.; SILVA, N. M. A. da; PONZETTO, J. M.; PAIVA, S. R.; CAETANO, A. R.. |
| The cachara (Pseudoplatystoma reticulatum) is a Neotropical freshwater catfish from family Pimelodidae (Siluriformes) native to Brazil. The species is of relative economic importance for local aquaculture production and basic biological information is under development to help boost efforts to domesticate and raise the species in commercial systems. The complete cachara mitochondrial genome was obtained by assembling Illumina RNA-seq data from pooled samples. The full mitogenome was found to be 16,576 bp in length, showing the same basic structure, order, and genetic organization observed in other Pimelodidae, with 13 protein-coding genes, 2 rNA genes, 22 trNAs, and a control region. Observed base composition was 24.63% T, 28.47% C, 31.45% A, and 15.44% G.... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Cachara; Peixe-gato; Mitogenome; Aquicultura; Filogenia; Melhoramento genético animal; Peixe de água doce; RNA; Pseudoplatystoma reticulatum; Mitochondrial genome; Siluriformes; Phylogeny; Catfish; Single nucleotide polymorphism. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074198 |
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| Teixeira,S.M.R.. |
| The study of mechanisms which control gene expression in trypanosomatids has developed at an increasing rate since 1989 when the first successful DNA transfection experiments were reported. Using primarily Trypanosoma brucei as a model, several groups have begun to elucidate the basic control mechanisms and to define the cellular factors involved in mRNA transcription, processing and translation in these parasites. This review focuses on the most recent studies regarding a subset of genes that are expressed differentially during the life cycle of three groups of parasites. In addition to T. brucei, I will address studies on gene regulation in a few species of Leishmania and the results obtained by a much more limited group of laboratories studying gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma; Leishmania; Gene expression; RNA; Antigenic variation; VSG. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200001 |
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| Registros recuperados: 65 | |
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