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| MACHADO, A. F. de F.; ANTONIASSI, R.; GUEDES, A. M. M.; WILHELM, A. E.. |
| Os tococromanóis são normalmente analisados por cromatografia líquida de alta eficiência (HPLC) em fase normal, conforme indicado pelos métodos oficiais. Considerando que a cromatografia em fase reversa (RP) apresenta algumas vantagens em relação à fase normal, neste trabalho um método baseado em RP-HPLC foi adaptado e validado para análise de azeites de oliva brasileiros. Para garantir a seletividade e a sensibilidade do método, foi selecionado o detector de fluorescência. O método apresentou boa linearidade (R2?0,995), com limites de detecção e quantificação de 2,5?0,7 ng e 7,4?2,1 ng de ?-tocoferol, respectivamente. O teor de tocoferóis totais nas amostras de azeite analisadas foi de 146-385 mg/kg, sendo ?-tocoferol o principal componente (78-96 %). A... |
| Tipo: Comunicado Técnico (INFOTECA-E) |
Palavras-chave: HPLC-FLR; RP-HPLC; Azeites brasileiros; Tecnologia de Alimento; Vitamina E; Azeite. |
| Ano: 2018 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1104313 |
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| Marques-Marinho,Flávia Dias; Zanon,José Carlos da Costa; Sakurai,Emilia; Reis,Ilka Afonso; Lima,Angélica Alves; Vianna-Soares,Cristina Duarte. |
| Simvastatin is commercially available as tablets and compounded capsules in Brazil. Very few reports regarding these capsules' quality, and consequently their efficacy, are available. The pharmaceutical quality of 30 batches of 20 mg simvastatin capsules from the market was evaluated by weight determination, content uniformity, disintegration (Brazilian Pharmacopeia), assay and dissolution test (USP32 tablet monograph). A HPLC method was developed for assay, content uniformity and dissolution test, and specifications were also established. Out of the 30 batches evaluated, 29 showed capsule disintegration within 45 min and individual weight variation was within ±10% or ±7.5% relative to average weight, for < or > 300 mg, respectively. Only 27... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compounded simvastatin capsules; Quality control; RP-HPLC. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300007 |
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| MAINARDES,RUBIANA M; PALMIRA D. GREMIÃO,MARIA. |
| The development and validation of a simple and accurate method based on HPLC with ultraviolet detection for the quantification of zidovudine in rat plasma and its application to a pharmacokinetic study following a single intranasal dose zidovudine is described. Zidovudine was extracted from the plasma using a single-step deproteinization. Chromatographic separation of zidovudine from interfering components was achieved with a C-18 reverse phase column, a mobile phase consisting of a mixture of sodium acetate buffer (55mM) with pH adjusted to 7.0 and acetonitrile (91:9 v/v) and UV detection set at 265 nm. The method was linear from 100 to 10000 ng.mL"¹ (r² > 0.9995), and zidovudine had a mean recovery from plasma of 92.8%. The coefficient of variation of... |
| Tipo: Journal article |
Palavras-chave: RP-HPLC; Validation; AZT; Pharmacokinetics; Intranasal delivery. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300010 |
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