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| Registros recuperados: 100 | |
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| MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
| Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
| Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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| Chauffaille,M.L.L.F.; Figueiredo,M.S.; Beltrani,R.; Antunes,S.V.; Yamamoto,M.; Kerbauy,J.. |
| Acute promyelocytic leukemia (AML M3) is a well-defined subtype of leukemia with specific and peculiar characteristics. Immediate identification of t(15;17) or the PML/RARA gene rearrangement is fundamental for treatment. The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. Bone marrow samples were submitted to karyotype G-banding, FISH and RT-PCR. At diagnosis, cytogenetics was successful in 10 of 12 samples, 8 with t(15;17) and 2 without. FISH was positive in 11/12 cases (one had no cells for analysis) and positivity varied from 25 to 93% (mean: 56%). RT-PCR was done... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acute promyelocytic leukemia; Karyotype; FISH; RT-PCR; PML/RARA genem rearrangement. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600006 |
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| Almeida-Val,Vera Maria Fonseca; Oliveira,Alice Reis; Silva,Maria de Nazaré Paula da; Ferreira-Nozawa,Monica Stropa; Araújo,Roziete Mendes; Val,Adalberto Luis; Nozawa,Sérgio Ricardo. |
| Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such as erythropoietin, transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis (the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon; Fishes; Gene expression; RT-PCR; LDH-A; Hypoxia; Anoxia. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200025 |
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| Meira,DA; Almeida,RAMB; Barbosa,AN; de Souza,LR; Olivo,TET; Henriques,RMS; Golim,MA; Araújo Jr,JP; Nagoshi,LR; Orikaza,CM; Calvi,SA. |
| A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RT-PCR; ELISA; Cytokines; HIV; AIDS; HAART; Apoptosis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011 |
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| Negrão,F.J.; Alfieri,A.A.; Alfieri,A.F.. |
| Urine and leucocytes were comparatively evaluated as clinical samples for ante mortem detection of the canine distemper virus (CDV) by a reverse transcription-polymerase chain reaction (RT-PCR) assay. One hundred and eighty eight dogs with clinical symptoms of distemper, were distributed in three groups. The group A was constituted of 93 dogs with systemic signs of distemper; the group B by 11 dogs with neurological signs, and the group C by 84 dogs that presented simultaneously systemic and neurological signs. In 66.5% (125/188) of the dogs was amplified an amplicon with 287 base pair of the CDV nucleoprotein gene. In 60.8% (76/125) of the animals the CDV was detected simultaneously in the urine and leucocytes, and in 39.2% (49/125) of the dogs just a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Cinomose; Diagnóstico; RT-PCR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100042 |
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| Gioria,Ricardo; Braga,Renato de Souza; Krause-Sakate,Renate; Roullier,Caroline; Rosa,Daniel Dias; Moura,Monika Fecury; Souza-Dias,José Alberto Caram de; Sawazaki,Haiko Enok; Camargo,Luis Eduardo Aranha; Rezende,Jorge Alberto Marques. |
| Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potyvirus; RT-PCR; ELISA; Resistance gene. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000200017 |
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| Macedo, Carla Isabel; Peixoto, Zélia Maria Pinheiro; Castilho, Juliana Galera; Oliveira, Rafael de Novaes; Rodrigues, Adriana Cândido; Achkar, Samira Maria. |
| Canine distemper (CD) is one of the most important infectious diseases in domestic dogs. In Brazil, CD is still the principal cause of mortality of dogs in some urban populations. Although different viral gene sequences have been identified for detecting the canine distemper virus (CDV), the N gene appears to be a better target for the amplification of specific fragments from all CDV strains. Using RT-PCR targeted to the CDV N gene, during the year 2014 we analyzed 190 central nervous system (CNS) samples of dogs from the state of São Paulo, Brazil, with symptoms suggestive of encephalitis and sent them to the Pasteur Institute for the diagnosis of rabies. Positivity for CD was over 50%, indicating that the disease remains an important cause of canine... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine Distemper; RT-PCR; Encephalitis; Diagnosis cinomose canina; RT-PCR; Encefalite; Diagnóstico. |
| Ano: 2016 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31032 |
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| AYALA VÁSQUEZ,MARILUZ; GONZÁLEZ JAIMES,PAOLA; GUTIÉRREZ,PABLO ANDRÉS; COTES,JOSÉ MIGUEL; MARÍN MONTOYA,MAURICIO. |
| El tomate de árbol (Solanum betaceum) es una fruta andina altamente nutritiva, con propiedades organolépticas únicas para el procesamiento industrial y el mercado internacional. En Antioquia, Colombia, este cultivo presenta diferentes problemas fitosanitarios, siendo especialmente limitante la Virosis, causada por un complejo viral del que hacen parte entre otras, especies del género Potyvirus. En este trabajo se evaluaron mediante pruebas de ELISA los niveles de incidencia de este grupo viral en cultivos de siete zonas del departamento de Antioquia y se determinó la identidad taxonómica de dos de los potyvirus asociados al cultivo. Las pruebas serológicas muestran la presencia de potyvirus en seis de las siete zonas evaluadas, alcanzando niveles... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Potyvirus; PVY; RT-PCR; Tamarillo. |
| Ano: 2010 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300011 |
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| Maciel,Sheila Conceição; Silva,Ricardo Ferreira da; Reis,Marcelo Silva; Jadão,Adriana Salomão; Rosa,Daniel Dias; Giampan,José Segundo; Kitajima,Elliot Watanabe; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo. |
| Catharanthus roseus is a perennial, evergreen herb in the family Apocynaceae, which is used as ornamental and for popular medicine to treat a wide assortment of human diseases. This paper describes a new potyvirus found causing mosaic symptom, foliar malformation and flower variegation in C. roseus. Of 28 test-plants inoculated mechanically with this potyvirus, only C. roseus and Nicotiana benthamiana developed systemic mosaic, whereas Chenopodium amaranticolor and C. quinoa exhibited chlorotic local lesions. The virus was transmitted by Aphis gossypii and Myzus nicotianae. When the nucleotide sequence of the CP gene (768nt) was compared with other members of the Potyviridae family, the highest identities varied from 67 to 76 %. For the 3' UTR (286nt),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RT-PCR; Apocynaceae; Periwinkle; Diagnose; Genome sequencing. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600013 |
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| Camargos,A.; Wohlres-Viana,S.; Costa,I.F.; Camargo,L.S.; Ferreira,J.C.; Ramos,A.A.; Oba,E.. |
| ABSTRACT The present study investigated the hormonal profile and expression of prostaglandin F2α (PGF2α), oxytocin and estrogen receptors in uterine tissues of postpartum cows treated with cloprostenol. Twenty Holstein-Zebu crossbred cows were treated with saline solution (treatment CONT) or cloprostenol (treatment CLO), both administered two and five days postpartum. Blood samples were collected on days two, seven, 14, 21 and 28 postpartum for progesterone, PGF2α metabolite (PGFM) and estradiol determination, and endometrial biopsy was performed in order to quantify the expression of oxytocin receptor (OXTR), prostaglandin F receptor (PTGFR) and estrogen receptor 1 (ERS1) genes. In the CLO treatment, expression of OXTR was reduced (P<0.05) but no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PTGFR; ESR1; Progesterone; Estradiol; RT-PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000400821 |
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| Shu,Canwei; Sun,Si; Chen,Jieling; Chen,Jianyi; Zhou,Erxun. |
| Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and contain a large quantity of polysaccharides, lipids, proteins and pigments. In order to extract high-quality total RNA from the sclerotia of R. solani, five methods, including E.Z.N.A.™ Fungal RNA Kit, sodium dodecyl sulfate (SDS)-sodium borate, SDS-polyvinylpyrrolidone (PVP), guanidinium thiocyanate (GTC) and modified Trizol, were compared in this study. Results The electrophoresis results showed that it failed to extract total RNA from the... |
| Tipo: Journal article |
Palavras-chave: Polyvinylpyrrolidone; Rhizoctonia solani; RT-PCR; Sclerotia; Sodium dodecyl sulfate; Total RNA extraction. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100009 |
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| Dubois, Eric. |
| Les rotavirus font partie des principaux virus entériques humains susceptibles d'être rencontrés dans l'environnement. Leur mise en évidence par culture cellulaire dans des prélèvements est longue et peu sensible, ce qui explique le manque de données sur la contamination du littoral. La RT-seminested PCR a été développée pour pallier ces inconvénients. Après une mise au point méthodologique cette technique a été utilisée lors d'études in vitro et in situ. Les études in vitro sur le devenir des rotavirus en eau de mer, suivi par RT-PCR quantitative et par multiplication en culture cellulaire, montrent que dans des conditions proches de celles du milieu naturel (eau de mer non stérile et à une température relativement basse), la détection d'ARN viral peut... |
| Tipo: Text |
Palavras-chave: Rotavirus; Contamination; Coquillages; Eaux de stations d'épuration; Echantillons cliniques; RT-PCR; RFLP; Epidémiologie moléculaire. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00442/55376/56894.pdf |
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| Decai,X; Zhiyong,Z; Bin,Z; Zhongcheng,H; Quanshu,W; Jing,L. |
| ABSTRACT Quantitative RT-PCR was applied to measure the relative expression levels of the adipose differentiation-related protein (ADFP) gene, fatty acid transport protein 1 (FATP1) gene and apolipoprotein B (ApoB) gene in subcutaneous fat, abdominal fat, liver and muscle at five growth stages (28, 49, 70, 91 and 112 d) to determine the effect of the expression ofthese genes on fat deposition in Daweishan Mini chickens.The relative expression of ADFP gene mRNA in the abdominal fat andthe liver was significantly different between 49 d and 70 d (p<0.05). The relative ApoB gene expression on 91d was higher in the liver, followed by muscles,subcutaneous fat, and abdominal fat, and was significantly higher in the liver than in the other three tissues. FATP1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ADFP; FATP1; ApoB; Daweishan Mini chicken; Fat traits; RT-PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100151 |
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| Registros recuperados: 100 | |
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