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Registros recuperados: 31
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A lethal effect associated with polymorphism of the NOR-bearing chromosomes in rainbow trout (Oncorhynchus mykiss) 74
Porto-Foresti,Fábio; Oliveira,Claudio; Gomes,Eduardo A.; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
Cytogenetic analysis of a rainbow trout stock showed that the nucleolar organizing regions were located subterminally on the long arm of a submetacentric chromosome pair and occurred as a single chromosomal segment (phenotype N1) or as two chromosomal segments separated by a short euchromatic segment (phenotype N2). Cytogenetic analysis also showed that there were N1N1 and N1N2 individuals but no N2N2 individuals. Analysis of the different includedphenotypes incluted that the population was not in Hardy-Weinberg equilibrium (chi² = 19.333; p < 0.01), and that a higher frequency of individuals had the N1N2 phenotype. Experimental crosses involving four males (two N1N1 and two N1N2) and four females (one N1N1 and three N1N2) yielded eight broods. There...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NOR polymorphism; Paracentric inversion; Fish cytogenetics; Rainbow trout.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100009
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Analysis of NOR distribution in cultivated and naturalized stocks of rainbow trout (Oncorhynchus mykiss) 74
Porto-Foresti,Fábio; Oliveira,Claudio; Tabata,Yara A.; Rigolino,Marcos G.; Foresti,Fausto.
In salmonid species, nucleolar organizing regions (NORs) have frequently been found on a single chromosome pair. However, some other, smaller yet active NORs have also been described. In the present study, NORs were found to be located in different positions on the chromosomes of 12 rainbow trout samples (11 cultivated and one naturalized stocks). Three phenotypes were found: 1) NORs located on two submetacentric chromosomes, in a subterminal position on the short arm (AA); 2) NORs located on two submetacentric chromosomes, in a subterminal position on the long arm (BB); and 3) NORs located on two submetacentric chromosomes, one in a subterminal position on the short arm, and the other in a subterminal position on the long arm (AB). Phenotype AA was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NOR polymorphism; Fish cytogenetics; Rainbow trout.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400009
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Analysis of the association between spawning time QTL markers and the biannual spawning behavior in rainbow trout (Oncorhynchus mykiss) 74
Colihueque,Nelson; Cárdenas,Rosy; Ramírez,Lorena; Estay,Francisco; Araneda,Cristian.
The rainbow trout is a salmonid fish that occasionally exhibits broodstocks with biannual spawning behavior, a phenomenon known as a double annual reproductive cycle (DARC). Spawning time quantitative trait loci (SPT-QTLs) affect the time of the year that female rainbow trout spawn and may influence expression of the DARC trait. In this study, microsatellite markers linked and unlinked to SPT-QTLs were genotyped to investigate the underlying genetics of this trait. SPT-QTLs influenced the DARC trait since in two case-control comparisons three linked markers (OmyFGT12TUF, One3ASC and One19ASC) had significant levels of allelic frequency differentiation and marker-character association. Furthermore, alleles of One3ASC and One19ASC had significantly higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Association analysis; Biannual spawning; Microsatellite markers; Rainbow trout.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300032
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Anti-peptide antibodies: A tool for detecting IL-8 in salmonids 69
Santana,Paula; Palacios,Claudia; Narváez,Edgar; Guzmán,Fanny; Gallardo,José A; Mercado,Luis.
Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conserved in several vertebrate groups, pointing its importance. Analysis of the amino acid sequence of IL-8, projected from cDNA of Salmo salar, presents homology with the sequences of mammals, poultry and lamprey, indicating the presence of a homologous molecule in higher fish. However, there is no information at protein level, which allows characterizing the regulatory role of this molecule during the immune response in fish. Results: In this work, we designed and...
Tipo: Journal article Palavras-chave: Anti-IL-8; Fish antibodies; Fish chemokine; Rainbow trout; Synthetic peptide epitope.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500020
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Combined effects of water quality and stocking density on welfare and growth of rainbow trout (Oncorhynchus mykiss) 5
Person, Jeannine; Labbe, Laurent; Le Bayon, Nicolas; Severe, Armelle; Le Roux, Annick; Le Delliou, Herve; Quemener, Loic.
An 84-day experiment assessed the combined effects of two fresh water quality levels (H: 1.71 +/- 0.15 mg O(2)l(-1) and 0.28 +/- 0.01 mg l(-1) total ammonia nitrogen (T-AN), L: 5.15 +/- 0.07 mg O2l(-1) and 0.54 +/- 0.01 mg T- AN l(-1)) and 3 stabilized stocking densities: 24.8 +/- 0.2, 74.2 +/- 0.5 and 120.0 +/- 0.9 kg m(-3)) on rainbow trout. Fish were fed using demand feeders with rewards proportional to stocking density. Mass increase was significantly affected by water quality and stocking density, being highest in H water and the lowest at 120 kg m(-3). There was no significant difference in final weight between 25 and 74 kg m(-3), but at 120 kg m(-3) it was 27% and 19% lower in H and L water respectively than at 25 kg m(-3). Feed intake (FI) from day...
Tipo: Text Palavras-chave: Growth; Welfare; Stocking density; Water quality; Rainbow trout.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4555.pdf
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Comparison of brown trout (Salmo trutta) reared in fresh water and sea water to freshwater rainbow trout (Oncorhynchus mykiss) .1. Growth and nitrogen balance 5
Dosdat, Antoine; Metailler, Robert; Desbruyeres, Elisabeth; Huelvan, Christine.
Brown trout and rainbow trout (average weight 100 g) were reared in fresh water at 12 degrees C under the same conditions before transfer of brown trout to sea water, in order to compare nitrogen utilisation in the two species. Apparent protein digestibility (ADC), nitrogen (ammonia and urea) excretion, protein productive value (PPV) and actual observed nitro en mass balance were determined. Rainbow trout raised in fresh water had a higher growth rate (1.1 vs 0.8%. d(-1)), better food conversion ratio (0.7 vs 1.0), better ADC (91 vs 85%) and PPV (45 vs 35%) and lower ammonia excretion rates than brown trout reared in fresh water. Transferring brown trout to sea water induced lower PPV (30%) and ammonia and urea excretion. Salinity did not modify metabolic...
Tipo: Text Palavras-chave: Truite commune; Truite arc-en-ciel; Excrétion; Ammoniaque; Urée; Digestibilité; Croissance; Rétention protéique; Rainbow trout; Brown trout; Excretion; Ammonia; Urea; Digestibility; Growth; Protein productive value.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00188/29885/28342.pdf
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Comparison of fatty acid content of fresh and frozen fillets of rainbow trout (Oncorhynchus mykiss) Walbaum 52
Chávez-Mendoza,Celia; García-Macías,José Arturo; Alarcón-Rojo,Alma Delia; Ortega-Gutiérrez,Juan Ángel; Holguín-Licón,Celia; Corral-Flores,Gabriela.
The aim of this study was to evaluate and compare the percentage content of fatty acids (FA) of 54 rainbow trout fresh and frozen fillets. Frozen fillets were stored at -15ºC for 45 and 90 days. Saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA), omega-6 (n-6) and omega-3 (n-3) fatty acids were determined by gas chromatograph coupled to mass spectrometry. The results indicated that during frozen storage, SFA and MUFA content increased by 32.63 and 9.25%, respectively, while PUFA content decreased by 25.3%, n-6 by 12.4% and n-3 by 32.55%. These changes were more significant (P ≤ 0.05) during the first 45 d of storage. It was concluded that the frozen storage had a negative impact on meat quality of rainbow trout due to the reduction of PUFA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Frozen storage; Rainbow trout; Fatty acid profile.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100015
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Densité d'élevage chez la truite arc-en-ciel (Onchorynchus mykiss W.). Conséquences sur la qualité de la chair et l'état apparent 5
Sampietro Coloh, Sonia.
A triploid strain of rainbow trout (Onchorynchus mykiss) was reared in two experiments testing density effect on growth and flesh quality. Physiological index and corporal condition were also considered. Environmental  parameters were maintained identical between densities. Two fold 20, 50 and 80 Kg m·3 stocking densities were reared at 1.3% daily ration. No differences were found in fish growth neither in fish quality. A second experience compared two densities (50 and 110 kg m-3 ) and oxygen supplementation. Fish were fed ad libitum. Weight gain diminished at higher densities and oxygen supplementation had a favourable effect on fish growth. No differences were found in flesh quality parameters tested.
Tipo: Text Palavras-chave: Rainbow trout; Onchorynchus mykiss stocking density; Flesh quality; Fin condition; Body condition.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00443/55473/57003.pdf
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Déterminisme nutritionnel et génétique de la teneur en lipides musculaires chez la truite arc-en-ciel (Oncorhynchus mykiss) [Texte imprimé] : étude par analyse de l'expression de gènes candidats, du protéome et du transcriptome du foie et du muscle 5
Kolditz, Catherine-inès.
The objective of the study was to identify genes and proteins that are involved in the control of muscle fat deposition in rainbow trout. We analyzed the combined effects exerted by genetic selection and dietary treatment, which are the two main factors that can be used to manage body fat content. Two lines of rainbow trout, obtained after 3 generations of divergent selection for high or low muscle fat content, were fed diets containing either 10% or 23% lipids (% dry matter), for six months. We analyzed the activity and gene expression of key enzymes involved in energy utilization, and performed a more global approach through transcriptome (nylon microarray) and proteome (twodimensional electrophoresis) analysis. We analyzed the liver, which is the centre...
Tipo: Text Palavras-chave: Proteomics; Transcriptomics; Selective breeding; Dietary energy; Rainbow trout; Protéome; Transcriptome; Sélection génétique; Apport énergétique alimentaire; Truite arc en ciel.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-6794.pdf
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Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss) liver 5
Panserat, S.; Hortopan, G. A.; Plagnes-juan, E.; Kolditz, C.; Lansard, M.; Skiba-cassy, S.; Esquerre, D.; Geurden, I.; Medale, Francoise; Kaushik, Sadasivam; Corraze, Genevieve.
Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100% plant-based diet (0% FM, 0% FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A...
Tipo: Text Palavras-chave: Rainbow trout; Transcriptome; Metabolism; Liver; Vegetable products; Marine ingredients.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6684.pdf
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Discrimination of bile acids by the rainbow trout olfactory system: Evidence as potential pheromone 48
GIAQUINTO,PERCILIA C; HARA,TOSHIAKI J.
Electro-olfactogram recording was used to determine whether the olfactory epithelium of adult rainbow trout is specifically sensitive to bile acids, some of which have been hypothesized to function as pheromones. Of 38 bile acids that had been pre-screened for olfactory activity, 6 were selected. The rainbow trout-specific bile acids, taurocholic acid (TCA), and taurolithocholic acid 3-sulfate (TLS) were the most potent compounds tested. TLS had a distinctive dose-response curve. Cross-adaptation experiments demonstrated that sensitivity to bile acids is attributable to at least 3 independent classes of olfactory receptor sites. Our data suggest that bile acids are discriminated by olfaction in rainbow trout, supporting the possibility that these compounds...
Tipo: Journal article Palavras-chave: Bile acids; Chemical signals; Electro-olfactogram; Olfaction; Pheromones; Rainbow trout.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100005
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Effects of autoclaving on the apparent digestibility coefficient of dehulled pea seed meal (Pisum sativum L.) in rainbow trout (Oncorhynchus mykiss W.) 118
Hernández,Adrián; Bórquez,Aliro; Alcaíno,Leonardo; Morales,Jorge; Dantagnan,Patricio; Saez,Patricio.
The effect of autoclaving on the nutrients' apparent digestibility coefficient (ADC), digestible protein and energy of pea seed meal (P. sativum) fed to Rainbow trout (O. mykiss) was examined. Two samples of the pea meal were autoclaved at 121°C and 1.1 atm for 5 min (5'APM) or 15 min (15'APM), respectively. A third sample, used as control, was not treated (RPM). One reference diet (Basal diet) and 3 experimental diets were elaborated and labelled based on autoclaving time applied to the ingredient (RPM, 5'APM and 15'APM). The four diets were assigned using a completely randomised design, with each treatment having three replicates. 12 tanks were stocked each with 15 trouts with an average weight of 235 ± 10.4 g. Faeces were collected over a 7-day period...
Tipo: Journal article Palavras-chave: Autoclaving; Apparent digestibility coefficient; Pea seed meal; Rainbow trout.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300003
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Effects of dietary propolis and vitamin E on growth performance and antioxidant status in blood of juvenile Rainbow trout, Oncorhynchus mykiss (Teleostei: Salmoniformes) under different flow rates 103
Kelestemur,Gülüzar Tuna; Seven,Pinar Tatli; Yilmaz,Seval.
The present study investigated the effects of propolis and vitamin E supplementation in diets of juvenil rainbow trout subjected to two different flow rates with or without flow stress (0.9 and 2.1 l/min, respectively) on final weigth (FW), condition factor (CF), feed conservation ratio (FCR), protein efficiency ratio (PER) and vitamin A, C and E concentrations and malondialdehyde (MDA) levels in serum as well as plasma superoxide dismutase (SOD) activity. The experimental groups were as follows: group C was fed a basal diet, group P10 was fed a basal diet supplemented with 10 g propolis/kg, group P30 was fed a basal diet supplemented with 30 g propolis/kg, group E60 was fed a basal diet supplemented with 60 mg vitamin E/kg. CF and PER were not different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipid peroxidation; Rainbow trout; Superoxide dismutase; Vitamin E.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702012000200001
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Effects of rapeseed meal-glucosinolates on thyroid metabolism and feed utilization in rainbow trout 5
Burel, Christine; Boujard, Thierry; Kaushik, Sadasivam; Boeuf, Gilles; Mol, K; Van Der Geyten, S; Darras, V; Kuhn, E; Pradet Balade, B; Querat, B; Quinsac, A; Krouti, M; Ribaillier, D.
Two rapeseed meals (RM1 and RM2), containing glucosinolates at a concentration of 26 and 40 mumol/g, respectively, were incorporated at increasing levels (10, 20, and 30% for RM1 and 30 and 50% for RM2) in diets of juvenile rainbow trout. Disturbances in the thyroid axis appeared after 14 days of feeding (with a dietary incorporation level of 10%). The dietary supplementation with T-3 or iodine induced an increase in plasma T-3 levels, compared to that in fish fed the RM diets, and reduced the deleterious effect of RM on growth. When trout were reared in seawater, there was also a slight increase in thyroid hormone levels. TSH treatment had no effect on the thyroid hormone plasma levels. The incorporation of 30% of RM1, which induced a lower dietary...
Tipo: Text Palavras-chave: Rainbow trout; Iodine; TSH; Deiodinase activity; Thyroid function; Growth; Glucosinolates; Rapeseed.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-677.pdf
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Effet du taux et de la nature des lipides alimentaires sur les mécanismes intervenant dans la constitution des dépôts lipidiques (transport, captage, synthèse) chez la truite arc-en-ciel et le bar 5
Richard, Nadège.
Replacement of dietary fish oil by a blend of vegetable oils for more than a year does not affect growth, hepatic lipogenesis and tissue lipid uptake in rainbow trout and European seabass. However, this replacement decreases plasma cholesterol level in both species and LDL amount in rainbow trout. Such replacement does not affect muscle lipid content of fish but modifies fatty acid composition of fish flesh which reflects in most part that of dietary lipid source. Increasing dietary lipid level inhibits hepatic lipogenesis but does not modify tissue lipid uptake in seabass and in two trout lines selected for muscle lipid level. In seabass, dietary lipid level had a lower impact on growth and lipid metabolism than rearing temperature. In this species,...
Tipo: Text Palavras-chave: Seabass; Rainbow trout; Lipoproteins; Lipoprotein lipase; Lipogenesis; Dietary lipid; Truite arc en ciel; Lipoprotéine lipase; Lipoprotéines; Lipogenèse; Lipides alimentaires; Bar.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-2297.pdf
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Genetic variability in residual feed intake in rainbow trout clones and testing of indirect selection criteria 5
Grima, Laure; Quillet, E; Boujard, Thierry; Robert Granie, C; Chatain, Beatrice; Mambrini, M.
Little is known about the genetic basis of residual feed intake (RFI) variation in fish, since this trait is highly sensitive to environmental influences, and feed intake of individuals is difficult to measure accurately. The purpose of this work was (i) to assess the genetic variability of RFI estimated by an X-ray technique and (ii) to develop predictive criteria for RFI. Two predictive criteria were tested: loss of body weight during feed deprivation and compensatory growth during re-feeding. Ten heterozygous rainbow trout clones were used. Individual intake and body weight were measured three times at three-week intervals. Then, individual body weight was recorded after two cycles of a three-week feed deprivation followed by a three-week re-feeding....
Tipo: Text Palavras-chave: Selection; Indirect criteria; Residual feed intake; Clone; Rainbow trout.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4777.pdf
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Hematologia comparada entre diplóides e triplóides de truta arco-íris, Oncorhynchus mykiss walbaum (Pisces, Salmonidae) 104
Ranzani-Paiva,Maria José Tavares; Tabata,Yara Aiko; Eiras,Augusta Cocuzza das.
This Study was carried out at the Salmoniculture Experimental Station "Dr. Ascanio de Faria", in Campos do Jordão, São Paulo. Brazil. The aim was to compared the hematology of triploid and diploid rainbow trout, Oncorhynchus mykiss Walbaum, 1792, by sex and gonadal maturation stages. Blood was obtained from 144 individuais for determinati-ons of: haemoglobin rate (Hb), haematocrit (Ht), erythrocyte count (Er), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MHC), mean corpuscular hemoglobin concentration (MCHC), and differential leucocyte counts. The ploid was determinated by measuring the erythrocyte major axis, in fresh and stainncd smears. Triploid rainbow trout had a large eiythrocyte diameter, mean erythrocyte volume and mean haemoglobin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oncorhynchus mykiss; Diploid; Triploid; Hematolology; Rainbow trout.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751998000400026
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Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss) 5
Panserat, Stéphane; Plagnes Juan, E; Breque, Jeanine; Kaushik, Sadasivam.
Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form, In gluconeogenic tissues such as liver, kidney and intestine,...
Tipo: Text Palavras-chave: Gluconeogenesis; Nutrition; Metabolism; Glucose; Oncorhynchus mykiss; Rainbow trout; Phosphoenolpyruvate carboxykinase.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-414.pdf
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How to assess fin damage in rainbow trout, Oncorhynchus mykiss? 5
Person, Jeannine; Le Bayon, Nicolas; Gros, Sylvie.
Fin condition was examined in 100-500 g hatchery-reared rainbow trout sampled in a large range of environmental conditions of an experimental farm. A method that is simple to use, requires little training, and does not need costly sophisticated equipment or reference to a model is proposed to assess fin erosion (defined as a decrease in size and profile alteration of fins). A procedure to reliably measure the length of the longest ray present in two selected fins (dorsal and pectoral) is described. Fin general profile is assessed using five levels of erosion based on the precise rank of intact or eroded rays to limit subjective bias. The suitability of the two descriptors of fin erosion under laboratory, farm or field conditions is discussed.
Tipo: Text Palavras-chave: Fin erosion; Oncorhynchus mykiss; Rainbow trout.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2585.pdf
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Lack of significant long-term effect of dietary carbohydrates on hepatic glucose-6-phosphatase expression in rainbow trout (Oncorhynchus mykiss) 5
Panserat, Stéphane; Medale, Francoise; Breque, Jeanine; Plagnes Juan, E; Kaushik, Sadasivam.
Hepatic glucose-6-phosphatase (G6Pase) plays an important role in glucose metabolism because it catalyzes the release of glucose to the circulatory system in the processes of glycogenolysis and gluconeogenesis. The present study was initiated to analyze the regulation of hepatic G6Pase expression by dietary carbohydrates in rainbow trout. The first step in our study was the identification of a partial G6Pase cDNA in rainbow trout that was highly homologous to that of mammals. Hepatic G6Pase activities and mRNA levels were measured in trout fed one of the experimental diets, with or without carbohydrates. We found no significant effect of intake of dietary carbohydrates on G6Pase Expression (mRNA and activity) 6 hours and 24 hours after feeding. These...
Tipo: Text Palavras-chave: Dietary carbohydrates; Rainbow trout; Fish nutrition; Glucose 6 phosphatase expression.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-696.pdf
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