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Registros recuperados: 76
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2-Styrylchromones: novel strong scavengers of reactive oxygen and nitrogen species IPB - Escola Superior Agrária
Gomes, Ana; Fernandes, Eduarda; Silva, Artur; Pinto, Diana; Santos, Clementina M.M.; Cavaleiro, José; Lima, José Costa.
2-Styrylchromones are a small group of naturally occurring chromones, vinylogues of flavones (2-phenylchromones). Natural and synthetic 2-styrylchromones have been tested in different biological systems, showing activities with potential therapeutic applications. In particular, the potential and hitherto understudied antioxidant behavior of these compounds has been raised as a matter of interest. Thus the present work consisted in the study of the in vitro scavenging activities for reactive oxygen species (ROS) and reactive nitrogen species (RNS) of various 2-styrylchromone derivatives and structurally similar flavonoids. Some of the studied 2-styrylchromones proved to be extremely efficient scavengers of the different ROS and RNS, showing, in some cases,...
Tipo: Article Palavras-chave: 2-Styrylchromones; Reactive oxygen species; Reactive nitrogen species; Scavenging activity; Antioxidant activity.
Ano: 2007 URL: http://hdl.handle.net/10198/3922
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A mechanistic view of mitochondrial death decision pores BJMBR
Belizário,J.E.; Alves,J.; Occhiucci,J.M.; Garay-Malpartida,M.; Sesso,A..
Mitochondria increase their outer and inner membrane permeability to solutes, protons and metabolites in response to a variety of extrinsic and intrinsic signaling events. The maintenance of cellular and intraorganelle ionic homeostasis, particularly for Ca2+, can determine cell survival or death. Mitochondrial death decision is centered on two processes: inner membrane permeabilization, such as that promoted by the mitochondrial permeability transition pore, formed across inner membranes when Ca2+ reaches a critical threshold, and mitochondrial outer membrane permeabilization, in which the pro-apoptotic proteins BID, BAX, and BAK play active roles. Membrane permeabilization leads to the release of apoptogenic proteins: cytochrome c, apoptosis-inducing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondrial outer membrane permeabilization; Permeability transition pore; Cytochrome c; Reactive oxygen species; Caspases; BCL-2.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800001
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Abscisic acid and the antioxidant system are involved in germination of Butia capitata seeds Acta Botanica
Bicalho,Elisa Monteze; Santos,Talita R. Silva dos; Garcia,Queila Souza.
ABSTRACT Seed germination is an important step for plants without vegetative propagation and is a physiological process that begins with specific environmental cues resulting in biochemical responses. Breaking-dormancy is necessary to study germination in dormant seeds with asynchronous germination. We investigated the processes of breaking dormancy and germination of Butia capitata (Arecaceae) seeds, in which germination is slow and asynchronous, by operculum removal. This treatment increased germination of B. capitata to 90 %. Embryos of dry, imbibed, 24-hours post-operculum removal and early-germinated seeds were collected for biochemical analysis of the following: quantification of abscisic acid (ABA) and hydrogen peroxide (H2O2), activities of...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Breaking-dormancy; Haustorium; Hormones; Palm seeds; Operculum; Physiological dormancy; Reactive oxygen species.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100174
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Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli BABT
Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de.
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020
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Ambient particulate air pollution from vehicles promotes lipid peroxidation and inflammatory responses in rat lung BJMBR
Pereira,C.E.L.; Heck,T.G.; Saldiva,P.H.N.; Rhoden,C.R..
Oxidative stress plays a major role in the pathogenesis of particle-dependent lung injury. Ambient particle levels from vehicles have not been previously shown to cause oxidative stress to the lungs. The present study was conducted to a) determine whether short-term exposure to ambient levels of particulate air pollution from vehicles elicits inflammatory responses and lipid peroxidation in rat lungs, and b) determine if intermittent short-term exposures (every 4 days) induce some degree of tolerance. Three-month-old male Wistar rats were exposed to ambient particulate matter (PM) from vehicles (N = 30) for 6 or 20 continuous hours, or for intermittent (5 h) periods during 20 h for 4 consecutive days or to filtered air (PM <10 µm; N = 30). Rats...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxidative stress; Reactive oxygen species; Lipid peroxidation; Particulate air pollution; Lung inflammation; Acute effects.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000008
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An overview of chagasic cardiomyopathy: pathogenic importance of oxidative stress Anais da ABC (AABC)
Zacks,Michele A.; Wen,Jian-Jun; Vyatkina,Galina; Bhatia,Vandanajay; Garg,Nisha.
There is growing evidence to suggest that chagasic myocardia are exposed to sustained oxidative stress-induced injuries that may contribute to disease progression. Pathogen invasion- and replication-mediated cellular injuries and immune-mediated cytotoxic reactions are the common source of reactive oxygen species (ROS) in infectious etiologies. However, our understanding of the source and role of oxidative stress in chagasic cardiomyopathy (CCM) remains incomplete. In this review, we discuss the evidence for increased oxidative stress in chagasic disease, with emphasis on mitochondrial abnormalities, electron transport chain dysfunction and its role in sustaining oxidative stress in myocardium. We discuss the literature reporting the consequences of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagasic cardiomyopathy; Reactive oxygen species; Inflammation; Mitochondria; Oxidant/antioxidant status; Oxidative damage.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400009
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Angiotensin II stimulates MCP-1 production in rat glomerular endothelial cells via NAD(P)H oxidase-dependent nuclear factor-kappa B signaling BJMBR
Pan,Q.; Yang,X.-H.; Cheng,Y.-X..
Angiotensin II (Ang II) plays a crucial role in the pathogenesis of renal diseases. The objective of the present study was to investigate the possible inflammatory effect of Ang II on glomerular endothelial cells and the underlying mechanism. We isolated and characterized primary cultures of rat glomerular endothelial cells (GECs) and observed that Ang II induced the synthesis of monocyte chemoattractant protein-1 (MCP-1) in GECs as demonstrated by Western blot. Ang II stimulation, at concentrations ranging from 0.1 to 10 µm, of rat GECs induced a rapid increase in the generation of reactive oxygen species as indicated by laser fluoroscopy. The level of p47phox protein, an NAD(P)H oxidase subunit, was also increased by Ang II treatment. These effects of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ang II; Glomerular endothelial cells; MCP-1; Reactive oxygen species; AT1 receptor blocker; NF-κB.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600009
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Antigenotoxic activity and antioxidant properties of organic and aqueous extracts of pequi fruit (Caryocar brasiliense Camb.) pulp Genet. Mol. Biol.
Miranda-Vilela,Ana L.; Resck,Inês S.; Grisolia,Cesar K..
The daily consumption of natural antioxidants protects against oxidative damage caused by reactive oxygen species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The pulp of pequi (Caryocar brasiliense Camb.) fruit, a tree native to the Brazilian savannah, contains several compounds with antioxidant properties, including carotenoids, vitamin C, phenolic compounds such as flavonoids, saponins and tannins, and essential oils. In this work, we examined the ability of organic and aqueous extracts of pequi fruit pulp to protect against the genotoxicity induced by two antineoplastic drugs, cyclophosphamide (CP) and bleomycin (BLM). Micronucleus tests with mouse bone marrow cells and single-cell gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antigenotoxic; Antioxidant; Caryocar brasiliense; Comet assay; Micronucleus; Pequi pulp extracts; Reactive oxygen species; TBARS assay.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500025
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Antioxidant and free radical scavenging effects in extracts of the medicinal herb Achyrocline satureioides (Lam.) DC. ("marcela") BJMBR
Desmarchelier,C.; Coussio,J.; Ciccia,G..
Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010
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Antioxidant response of cowpea co-inoculated with plant growth-promoting bacteria under salt stress BJM
Santos,Alexandra de Andrade; Silveira,Joaquim Albenísio Gomes da; Bonifacio,Aurenivia; Rodrigues,Artenisa Cerqueira; Figueiredo,Márcia do Vale Barreto.
Abstract Soil salinity is an important abiotic stress worldwide, and salt-induced oxidative stress can have detrimental effects on the biological nitrogen fixation. We hypothesized that co-inoculation of cowpea plants with Bradyrhizobium and plant growth-promoting bacteria would minimize the deleterious effects of salt stress via the induction of enzymatic and non-enzymatic antioxidative protection. To test our hypothesis, cowpea seeds were inoculated with Bradyrhizobium or co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and then submitted to salt stress. Afterward, the cowpea nodules were collected, and the levels of hydrogen peroxide; lipid peroxidation; total, reduced and oxidized forms of ascorbate and glutathione; and superoxide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant-bacteria interactions; Reactive oxygen species; Salinity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300513
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Antioxidant system response induced by aluminum in two rice cultivars Braz. J. Plant Physiol.
Ribeiro,Cleberson; Cambraia,José; Peixoto,Paulo Henrique Pereira; Fonseca Júnior,Élcio Meira da.
The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2•- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2•- only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aluminum; Enzymes; Reactive oxygen species; Oryza sativa; Oxidative stress.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200004
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Antioxidative enzymes of Cucumis sativus seeds are modulated by Leucaena leucocephala extracts Biological Sciences
Ribeiro, Vandjore de Mattos; Spiassi, Ariane; Marcon, Thaís Regina; Lima, Gislaine Piccolo de; Corsato, Jaqueline Malagutti; Fortes, Andréa Maria Teixeira.
 This study aimed to analyze oxidative stress produced in cucumber seeds and seedlings when exposed to aqueous extract from dried leaves of leucaena, as well as its effect on the germination behavior, early growth and the antioxidant enzymes activity. It was evaluated the percentage, the speed index, the average time, the frequency and germination synchronization, the root length and shoot, as well as the catalase and peroxidase enzymes activity. There was no significant inhibitory effect of the leaf extracts on the germination percentage. However, there was delay in the seeds germination, as the extract proportion increased. A stimulatory effect of the extract compared to the shoot length was observed, however the root growth was significantly reduced....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Botânica allelopathy; Reactive oxygen species; Antioxidant enzymes. Fisiologia Vegetal.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34801
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Arachidonic acid triggers an oxidative burst in leukocytes BJMBR
Pompeia,C.; Cury-Boaventura,M.F.; Curi,R..
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013
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Atrazine and Mesotrione-Induced Oxidative Stress and Impact on Antioxidant Enzymes and Chlorophyll Contents in Bermudagrass Planta Daninha
WANG,Y.; YU,J.; ZHOU,B.; SAPKOTA,S.; WEI,F.; WANG,Z..
ABSTRACT: The effect of atrazine, mesotrione, and joint activity of atrazine plus mesotrione on pigment, lipid peroxidation, and antioxidant enzyme activity was studied. Atrazine and mesotrione treatments significantly reduced chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoid concentrations, and protein content in bermudagrass (Cynodon dactylon L.) plants, whereas they significantly enhanced lipid peroxidation. The treatment of atrazine plus mesotrione caused greater phototoxic effect on bermudagrass than either herbicide alone, which was evident from the significantly decreased membrane stability noted as a function of the enhanced singlet oxygen and malondialdehyde (MDA) contents, as well as from the greater reduction in Chl a, Chl b, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Carotenoids; Herbicide; Reactive oxygen species.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100337
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Biomass Accumulation, Photosynthetic Pigments, Osmotic Adjustments and Antioxidant Activities of Leymus chinensis in Response to BA, BR, and GA Planta Daninha
XUE-FENG,Z.; JUN,L.V.; SHAHID,M.; ANJUM,S.A.; NA-JIA,L.; XIU-JUAN,H.; YU,X.; XIAO,W.; SAN-GEN,W..
ABSTRACT: Abiotic stresses and poor biomass accumulation are chief constraints to accomplish potential yield in Leymus chinensis. An experiment was conducted to improve biomass and correlation of biomass accumulating attributes with photosynthetic pigments, osmotic substances and antioxidants under foliar application of different plant growth substances. The experiment was conducted at inner Mongolia Xilinguole, China, using a Randomized Complete Block Design and 5 replications. The treatments consisted of water (control); BA5 = application of BA (6-benzylaminopurine) at 5 mg L-1; BA25 = application of BA at 25 mg L-1; BA50 = application of BA at 50 mg L-1; BR0.02 = application of BR (brassinosteroid) at 0.02 g L-1; BR0.2 = application of BR at 0.2 mg L 1;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Reactive oxygen species; Phytoregulators.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100256
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Biphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defense responses in resistant and susceptible coffee cultivars. Repositório Alice
RAMIRO, D. A.; ESCOUTE, J.; PETITOT, A.-S.; NICOLE, M.; MALUF, M. P.; FERNANDEZ, D..
The objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced ?pioneer? haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed ?secondary haustoria? which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coffea arabica; Pathogenesis-related genes; Quantitative real-time PCR; Reactive oxygen species.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/880368
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Candesartan inhibits inflammation through an angiotensin II type 1 receptor independent way in human embryonic kidney epithelial cells Anais da ABC (AABC)
YU,YING; JIANG,HAIFENG; NIU,YANGYANG; ZHANG,XIAOQIN; ZHANG,YINGYING; LIU,XI; QI,TAO; YU,CHEN.
Abstract: Besides stimulating vasoconstriction, Angiotensin II is also well known in inducing reactive oxygen species and promoting inflammatory phenotype switch via its type 1 receptor. In clinic, Angiotensin II type 1 (AT1) receptor blocker like candesartan has been widely applied as an antihypertensive medication. We previous have demonstrated that a higher dose of candesartan plays a protective role after kidney injury. However, whether candesartan could exhibit anti-inflammatory effects remains unclear. Here, by stimulating isolated human embryonic kidney epithelial cells with tumor necrosis factor-α (TNF-α), we observed the anti-inflammation capacity of candesartan ex vivo. It was found that pre-treat with candesartan significantly suppressed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II type 1 receptor blockers; Candesartan; Inflammation; Reactive oxygen species.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300701
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Characterization and mapping of a spotted leaf mutant in rice (Oryza sativa) Genet. Mol. Biol.
Xu,Xue; Zhang,Lili; Liu,Binmei; Ye,Yafeng; Wu,Yuejin.
Spotted leaf mutant belongs to a class of mutants that can produce necrotic lesions spontaneously in plants without any attack by pathogens. These mutants have no beneficial effect on plant productivity but provide a unique opportunity to study programmed cell death in plant defense responses. A novel rice spotted leaf mutant (spl30) was isolated through low-energy heavy ion irradiation. Lesion expression was sensitive to light and humidity. The spl30 mutant caused a decrease in chlorophyll and soluble protein content, with marked accumulation of reactive oxygen species (ROS) around the lesions. In addition, the spl30 mutant significantly enhanced resistance to rice bacterial blight (X. oryzae pv. oryzae) from China (C1-C7). The use of SSR markers showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene mapping; Reactive oxygen species; Rice (Orzya sativa); Rice bacterial blight; Spotted leaf mutant.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300013
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Cyclic voltammetric analysis of 2-styrylchromones: Relationship with the antioxidant activity IPB - Escola Superior Agrária
Gomes, Ana; Fernandes, Eduarda; Garcia, M. Beatriz; Silva, Artur; Pinto, Diana; Santos, Clementina M.M.; Cavaleiro, José; Lima, José Costa.
2-Styrylchromones (2-SC) are a chemical family of oxygen heterocyclic compounds, vinylogues of flavones (2-phenylchromones), whose occurrence in nature has been reported. Recently, several 2-SC derivatives were demonstrated to have antioxidant properties, namely, xanthine oxidase inhibition, hepatoprotection against pro-oxidant agents in cellular and non-cellular systems and scavenging activity against reactive oxygen and reactive nitrogen species (ROS and RNS). Considering these antioxidant properties, it may be hypothesised that the electrochemical redox behaviour of 2-SC contributes significantly to their activity. To test this hypothesis, the electrochemical behaviour of different 2-SC was studied, together with a number of flavonoids with well-known...
Tipo: Article Palavras-chave: 2-Styrylchromones; Cyclic voltammetry; Oxidation mechanism; Scavenging activity; Reactive oxygen species; Reactive nitrogen species.
Ano: 2008 URL: http://hdl.handle.net/10198/4011
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Defenses against ROS in crops and weeds: the effects of interference and herbicides. Repositório Alice
CAVERZAN, A.; PIASECKI, C.; CHAVARRIA, G.; STEWART JR, C. N.; VARGAS, L..
bitstream/item/203387/1/ID44704-2019v20n5IntJMolSci.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Herbicida; Erva Daninha; Herbicide resistance; Oxidative stress; Weeds; Reactive oxygen species.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113370
Registros recuperados: 76
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