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| Registros recuperados: 66 | |
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| Belizário,J.E.; Alves,J.; Occhiucci,J.M.; Garay-Malpartida,M.; Sesso,A.. |
| Mitochondria increase their outer and inner membrane permeability to solutes, protons and metabolites in response to a variety of extrinsic and intrinsic signaling events. The maintenance of cellular and intraorganelle ionic homeostasis, particularly for Ca2+, can determine cell survival or death. Mitochondrial death decision is centered on two processes: inner membrane permeabilization, such as that promoted by the mitochondrial permeability transition pore, formed across inner membranes when Ca2+ reaches a critical threshold, and mitochondrial outer membrane permeabilization, in which the pro-apoptotic proteins BID, BAX, and BAK play active roles. Membrane permeabilization leads to the release of apoptogenic proteins: cytochrome c, apoptosis-inducing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial outer membrane permeabilization; Permeability transition pore; Cytochrome c; Reactive oxygen species; Caspases; BCL-2. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800001 |
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| Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de. |
| Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020 |
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| Miranda-Vilela,Ana L.; Resck,Inês S.; Grisolia,Cesar K.. |
| The daily consumption of natural antioxidants protects against oxidative damage caused by reactive oxygen species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The pulp of pequi (Caryocar brasiliense Camb.) fruit, a tree native to the Brazilian savannah, contains several compounds with antioxidant properties, including carotenoids, vitamin C, phenolic compounds such as flavonoids, saponins and tannins, and essential oils. In this work, we examined the ability of organic and aqueous extracts of pequi fruit pulp to protect against the genotoxicity induced by two antineoplastic drugs, cyclophosphamide (CP) and bleomycin (BLM). Micronucleus tests with mouse bone marrow cells and single-cell gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antigenotoxic; Antioxidant; Caryocar brasiliense; Comet assay; Micronucleus; Pequi pulp extracts; Reactive oxygen species; TBARS assay. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500025 |
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| Desmarchelier,C.; Coussio,J.; Ciccia,G.. |
| Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010 |
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| Santos,Alexandra de Andrade; Silveira,Joaquim Albenísio Gomes da; Bonifacio,Aurenivia; Rodrigues,Artenisa Cerqueira; Figueiredo,Márcia do Vale Barreto. |
| Abstract Soil salinity is an important abiotic stress worldwide, and salt-induced oxidative stress can have detrimental effects on the biological nitrogen fixation. We hypothesized that co-inoculation of cowpea plants with Bradyrhizobium and plant growth-promoting bacteria would minimize the deleterious effects of salt stress via the induction of enzymatic and non-enzymatic antioxidative protection. To test our hypothesis, cowpea seeds were inoculated with Bradyrhizobium or co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and then submitted to salt stress. Afterward, the cowpea nodules were collected, and the levels of hydrogen peroxide; lipid peroxidation; total, reduced and oxidized forms of ascorbate and glutathione; and superoxide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant-bacteria interactions; Reactive oxygen species; Salinity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300513 |
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| Ribeiro,Cleberson; Cambraia,José; Peixoto,Paulo Henrique Pereira; Fonseca Júnior,Élcio Meira da. |
| The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2•- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2•- only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aluminum; Enzymes; Reactive oxygen species; Oryza sativa; Oxidative stress. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200004 |
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| Ribeiro, Vandjore de Mattos; Spiassi, Ariane; Marcon, Thaís Regina; Lima, Gislaine Piccolo de; Corsato, Jaqueline Malagutti; Fortes, Andréa Maria Teixeira. |
| This study aimed to analyze oxidative stress produced in cucumber seeds and seedlings when exposed to aqueous extract from dried leaves of leucaena, as well as its effect on the germination behavior, early growth and the antioxidant enzymes activity. It was evaluated the percentage, the speed index, the average time, the frequency and germination synchronization, the root length and shoot, as well as the catalase and peroxidase enzymes activity. There was no significant inhibitory effect of the leaf extracts on the germination percentage. However, there was delay in the seeds germination, as the extract proportion increased. A stimulatory effect of the extract compared to the shoot length was observed, however the root growth was significantly reduced.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Botânica allelopathy; Reactive oxygen species; Antioxidant enzymes. Fisiologia Vegetal. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34801 |
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| Pompeia,C.; Cury-Boaventura,M.F.; Curi,R.. |
| The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arachidonic acid; NADPH oxidase; Nitroblue tetrazolium; Leukocytes; Reactive oxygen species. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013 |
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| WANG,Y.; YU,J.; ZHOU,B.; SAPKOTA,S.; WEI,F.; WANG,Z.. |
| ABSTRACT: The effect of atrazine, mesotrione, and joint activity of atrazine plus mesotrione on pigment, lipid peroxidation, and antioxidant enzyme activity was studied. Atrazine and mesotrione treatments significantly reduced chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoid concentrations, and protein content in bermudagrass (Cynodon dactylon L.) plants, whereas they significantly enhanced lipid peroxidation. The treatment of atrazine plus mesotrione caused greater phototoxic effect on bermudagrass than either herbicide alone, which was evident from the significantly decreased membrane stability noted as a function of the enhanced singlet oxygen and malondialdehyde (MDA) contents, as well as from the greater reduction in Chl a, Chl b, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant; Carotenoids; Herbicide; Reactive oxygen species. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100337 |
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| XUE-FENG,Z.; JUN,L.V.; SHAHID,M.; ANJUM,S.A.; NA-JIA,L.; XIU-JUAN,H.; YU,X.; XIAO,W.; SAN-GEN,W.. |
| ABSTRACT: Abiotic stresses and poor biomass accumulation are chief constraints to accomplish potential yield in Leymus chinensis. An experiment was conducted to improve biomass and correlation of biomass accumulating attributes with photosynthetic pigments, osmotic substances and antioxidants under foliar application of different plant growth substances. The experiment was conducted at inner Mongolia Xilinguole, China, using a Randomized Complete Block Design and 5 replications. The treatments consisted of water (control); BA5 = application of BA (6-benzylaminopurine) at 5 mg L-1; BA25 = application of BA at 25 mg L-1; BA50 = application of BA at 50 mg L-1; BR0.02 = application of BR (brassinosteroid) at 0.02 g L-1; BR0.2 = application of BR at 0.2 mg L 1;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Reactive oxygen species; Phytoregulators. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100256 |
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| YU,YING; JIANG,HAIFENG; NIU,YANGYANG; ZHANG,XIAOQIN; ZHANG,YINGYING; LIU,XI; QI,TAO; YU,CHEN. |
| Abstract: Besides stimulating vasoconstriction, Angiotensin II is also well known in inducing reactive oxygen species and promoting inflammatory phenotype switch via its type 1 receptor. In clinic, Angiotensin II type 1 (AT1) receptor blocker like candesartan has been widely applied as an antihypertensive medication. We previous have demonstrated that a higher dose of candesartan plays a protective role after kidney injury. However, whether candesartan could exhibit anti-inflammatory effects remains unclear. Here, by stimulating isolated human embryonic kidney epithelial cells with tumor necrosis factor-α (TNF-α), we observed the anti-inflammation capacity of candesartan ex vivo. It was found that pre-treat with candesartan significantly suppressed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Angiotensin II type 1 receptor blockers; Candesartan; Inflammation; Reactive oxygen species. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300701 |
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| Hameed,Asiya; Qadri,Tabasum N.; Mahmooduzzafar,; Siddiqi,T.O.; Iqbal,M.. |
| Cadmium and Mercury induced varying responses in Abelmoschus esculentus L. in relation to enzymes (ascorbate peroxidase (APX, 1.11.1.11), catalase (CAT, 1.11.1.6), glutathione reductase (GR, 1.6.4.2) and superoxide dismutase (SOD, 1.15.1.1) which are most related to the levels of Hg and Cd applied and concentrations of thiol groups already present or induced upon treatment. In the present investigation varying concentrations of CdCl2 and HgCl2 (0, 0.05, 0.10, 0.50, 1 and 2mM respectively) applied to plant in the soil shows a significant increase in ascorbate peroxidase, glutathione reductase and superoxide dismutase activities, and the respective metal accumulation. It reveals a mechanism for constant detoxification of H2O2 that have to be associated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidative enzymes; Metals detoxification; Reactive oxygen species. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000100007 |
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| Pinto,S.C.C.; Almeida,D.S.; Alves,M.B.R.; Florez-Rodriguez,S.A.; Abreu Júnior,G.S.; Alves,N. Britto e; Celeghini,E.C.C.; Laskoki,L.M.; Souza,F.A.. |
| ABSTRACT The aim of this study was to evaluate the addition of vitamin C, reduced glutathione and the association thereof to the bovine semen cryopreservation extender. The ejaculate from nine bulls were divided into four fractions, each corresponding to a treatment, namely: control group-semen diluted with Tris-yolk extender; vitamin C group-semen diluted in Tris-yolk extender supplemented with vitamin C (2.5mmol/mL); glutathione group-semen diluted in Tris-yolk extender supplemented with reduced glutathione (2.5mmol/mL) and associated group-semen diluted in Tris-yolk extender supplemented with vitamin C (1.25mmol/mL) and reduced glutathione (1.25mmol/mL). Afterwards, the semen was packed into French straws and submitted to cryopreservation using... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reactive oxygen species; Sperm membranes; Spermatozoa. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100009 |
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| Paino,I.M.M.; Ximenes,V.F.; Fonseca,L.M. da; Kanegae,M.P.P.; Khalil,N.M.; Brunetti,I.L.. |
| The release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-steroidal anti-inflammatory drugs; Neutrophils; Myeloperoxidase; Hypochlorous acid; Reactive oxygen species; Chemiluminescence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400007 |
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| Registros recuperados: 66 | |
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