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| Muheem,Abdul; Shakeel,Faiyaz; Zafar,Sobiya; Jahangir,Mohammed Asadullah; Warsi,Musarrat Husain; Jain,Gaurav Kumar; Ahmad,Farhan Jalees. |
| ABSTRACT A novel, accurate, precise and economical stability indicating Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method, was developed and validated for the quantitative determination of ubidecarenone (UDC) in bulk drug, UDC marketed formulation and UDC loaded cubosomes (CBMs) nanocarriers through Response surface methodology (RSM) design with three factors and three levels was performed to optimize the chromatographic variables followed by forced degradation studies of UDC were performed to detect degradation peak. RP-HPLC separation was achieved using mobile phase consisting of Acetonitrile:Tetrahydrofuran:Deionised water in the ratio 55:42:3 and a flow rate of 1.0 mL/min was optimized with a standard retention time (Rt) of 2.15... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High Performance Liquid Chromatography/validation/stability; Response surface methodology (RSM); Ubidecarnenone (UDC)/evaluation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000400614 |
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| Meena,Ganga Sahay; Kumar,Nitin; Majumdar,Gautam Chandra; Banerjee,Rintu; Meena,Pankaj Kumar; Yadav,Vijesh. |
| The culture conditions viz. additional carbon and nitrogen content, inoculum size, age, temperature and pH of Lactobacillus acidophilus were optimized using response surface methodology (RSM) and artificial neural network (ANN). Kinetic growth models were fitted to cultivations from a Box-Behnken Design (BBD) design experiments for different variables. This concept of combining the optimization and modeling presented different optimal conditions for L. acidophilus growth from their original optimization study. Through these statistical tools, the product yield (cell mass) of L. acidophilus was increased. Regression coefficients (R²) of both the statistical tools predicted that ANN was better than RSM and the regression equation was solved with the help of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Response surface methodology (RSM); Artificial neural network (ANN); Genetic algorithms (GA); Box-behnken besign (BBD). |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100003 |
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| Teixeira,Sirlei Dias; Fiorio,Jhonatan Luiz; Galvan,Diego; Sefstrom,Carolina; Cogo,Priscila Morgana; Sales Junior,Valber; Rodrigues,Márcio Barreto; Hendges,Ana Paula Palaro Klein; Maia,Beatriz Helena L. de Noronha Sales; Benghi,Thalita Gilda Santos. |
| ABSTRACT: The extraction of essential oils obtained by the hydrodistillation of needles/twigs waste of Pinus taeda L. was optimized by applying response surface methodology (RSM), with 24 full factorial design, in order to improve oil essential production, and aggregate value to the production chain of pine wood. Through the model it was possible to ascertain the influence of the variables in the average amount of essential oil (0.1032 mL), being the variables analyzed: biomass - Bm (x1), extraction time - ET (x2), Bm:ET (x1x2) and sample size - SS: drying times - DT (x3x4). Only linear terms (biomass and extraction time) and your interaction demonstrated significant positive values (0.0344, 0.0206 and 0.0131). The major components of the essential oil... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pinus; Byproduct; Essential oil; Response surface methodology (RSM); Mathematical modeling. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100603 |
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| Espinosa,Wilfred Edilberto; Garzón,Luis Carlos Arturo; Medina,Oscar Julio. |
| ABSTRACT Due to their nutraceutical properties, the fruits of the species Vaccinium meridionale, which inhabit the Andean region, are of scientific interest. Microwave-assisted extraction has been applied to different vegetal matrices for to extract efficiently polyphenolic compounds. In this work we study in microwave assisted extraction processes, the effect on the extraction of total polyphenols in dried fruits of Vaccinium meridionale, using response surface methodology. The main objective of this research was to analyze the relationships between power, temperature, solid-liquid ratio, time and ethanol concentration in aqueous media on the effectiveness of total polyphenols recovery in dry fruits of Vaccinium meridionale. A central composite design... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Folin-Ciocalteu method; Response surface methodology (RSM); Nutraceutical properties. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000600701 |
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| Dong,Bing Xue; Zhang,Yu Hong; Li,Gang; Fang,Jin Tao; Zheng,Ji Huan; Mao,Run Qian. |
| ABSTRACT To seek a simple, rapid and sensitive Coprinus cinereus Peroxidase (CIP) activity assay, a convenient one-factor-at-a-time (OFAT) method and a response surface methodology (RSM) were used. The recombinant CIP expressed in Pichia pastoris was purified with the Ni-NTA spin column. Based on the results of catalytic efficiency (kcat/Km) analysis, 2,2'-azinobis (ethylbenzthiazoline -6-sulfonate) (ABTS) was selected as the optimal enzyme substrate. Results of the OFAT method showed that enzymatic reaction performed in 0.1 mol/L sodium acetate (pH 5.0) buffer in a 200-µl reaction mixture containing 0.5 mmol/L ABTS, 10 mmol/L hydrogen peroxide (H2O2), 49.7 ng CIP at 25°C gave an average CIP activity of 88 U/mL. The ABTS and H2O2 concentrations were then... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic activity assay; One-factor-at-a-time (OFAT) method; Response surface methodology (RSM); Coprinus cinereus peroxidase (CIP). |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100421 |
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