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Medeiros,M.A.; Canteras,N.S.; Suchecki,D.; Mello,L.E.A.M.. |
In laboratory animals, acupuncture needs to be performed on either anesthetized or, if unanesthetized, restrained subjects. Both procedures up-regulate c-Fos expression in several areas of the central nervous system, representing therefore a major pitfall for the assessment of c-Fos expression induced by electroacupuncture. Thus, in order to reduce the effect of acute restraint we used a protocol of repeated restraint for the assessment of the brain areas activated by electroacupuncture in adult male Wistar rats weighing 180-230 g. Repeated immobilization protocols (6 days, 1 h/day and 13 days, 2 h/day) were used to reduce the effect of acute immobilization stress on the c-Fos expression induced by electroacupuncture at the Zusanli point (EA36S). Animals... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acupuncture; Zusanli point; Immediate early genes; C-Fos; Restraint; Habituation. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200009 |
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Andrade,Maria Cristina Caldart de; Hünning,Paula Stieven; Pereira,Fabiana Quartiero; Dutra,Kadigia Pitol; Pigatto,João Antonio Tadeu. |
ABSTRACT: The purpose of this study was to verify the effect of the upper lip twitch restraint on intraocular pressure (IOP) of healthy horses. In this study, forty five Criollo horses, aged between two to 20 years (male or female) were evaluated with rebound tonometer, with and without upper lip twitch restraint. A previous ophthalmic examination was performed with Schirmer tear test, fluorescein test, slit lamp biomicroscopy and direct ophthalmoscopy in all horses. Only healthy animals with no ocular findings were used. The order of the IOP measurements (with or without twitch) and order of the eye (right or left) were randomized. Three measurements of each eye were made and the mean was calculated. Head position was kept above the heart level and no... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine; Intraocular pressure; Restraint; Tonovet.. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801486 |
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Padovan,C.M.; Guimarães,F.S.. |
Rodents submitted to restraint stress show decreased activity in an elevated plus-maze (EPM) 24 h later. The objective of the present study was to determine if a certain amount of time is needed after stress for the development of these changes. We also wanted to verify if behavioral tolerance of repeated daily restraint would be detectable in this model. Male Wistar rats were restrained for 2 h and tested in the EPM 1, 2, 24 or 48 h later. Another group of animals was immobilized daily for 2 h for 7 days, being tested in the EPM 24 h after the last restraint period. Restraint induced a significant decrease in the percent of entries and time spent in the open arms, as well as a decrease in the number of enclosed arm entries. The significant effect in the... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Stress; Restraint; Elevated plus-maze; Behavioral changes; Tolerance. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100011 |
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