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Double-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line. Repositório Alice
ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L..
Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G)...
Tipo: Artigo de periódico Palavras-chave: Rhabdovirus; Feijão; Phaseolus Vulgaris; Organismo Transgênico; Vírus; Mosaico Dourado; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants; Bean golden mosaic virus.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104582
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First isolation of hirame rhabdovirus from freshwater fish in Europe ArchiMer
Borzym, E.; Matras, M.; Maj-paluch, J.; Baud, M.; De Boisseson, C.; Talbi, Chiraz; Olesen, N. J.; Bigarre, L..
A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, Thymallus thymallus (L.), originating from a fish farm affected by a mortality episode in Poland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in Europe, nor to vesiculovirus-like species, except perch rhabdovirus (PRhV) with which it shared moderate serological relations. However, RT-PCR with PRhV probes gave negative results. To identify the virus, a random-priming sequence-independent single primer amplification was adopted. Surprisingly, two of the obtained sequences exhibited a high identity (>99%) with hirame rhabdovirus (HIRRV), a novirhabdovirus usually found in fish in marine Asiatic countries, for instance...
Tipo: Text Palavras-chave: Outbreak; Brown trout; Rhabdovirus; Sequence-independent single primer amplification; Molecular tracing; Grayling.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00197/30852/32727.pdf
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Predatory activity of Phytoseiid mites on the developmental stages of coffee ringspot mite (Acari: Phytoseiidae: Tenuipalpidae) Anais da SEB
Reis,Paulo R.; Teodoro,Adenir V.; Pedro Neto,Marçal.
By means of bioassays performed in arenas of 3 cm in diameter, manufactured with coffee leaves floating on water, the phases of the coffee ringspot mite Brevipalpus phoenicis (Geijskes) were studies on the preference by several developmental stages of the predaceous mites Euseius alatus DeLeon and Iphiseiodes zuluagai Denmark & Muma. The experiment was conducted in laboratory at 25 ± 2ºC, 70 ± 10% RH and 14 h photophase. The preferred stage of coffee ringspot vector virus was the larva followed by nymph and egg. Adults were least preyed on. In general, the most aggressive phase of the predators was the adult female followed by the nymph; the larval phase was the least aggressive. The average predation of E. alatus and I. zuluagai for the different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhabdovirus; Biological control; Flat-mite; Brevipalpus phoenicis; Euseius alatus; Iphiseiodes zuluaga.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80592000000300017
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Viral membrane fusion: is glycoprotein G of rhabdoviruses a representative of a new class of viral fusion proteins? BJMBR
Da Poian,A.T.; Carneiro,F.A.; Stauffer,F..
Enveloped viruses always gain entry into the cytoplasm by fusion of their lipid envelope with a cell membrane. Some enveloped viruses fuse directly with the host cell plasma membrane after virus binding to the cell receptor. Other enveloped viruses enter the cells by the endocytic pathway, and fusion depends on the acidification of the endosomal compartment. In both cases, virus-induced membrane fusion is triggered by conformational changes in viral envelope glycoproteins. Two different classes of viral fusion proteins have been described on the basis of their molecular architecture. Several structural data permitted the elucidation of the mechanisms of membrane fusion mediated by class I and class II fusion proteins. In this article, we review a number of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Membrane fusion; Fusion proteins; Rhabdovirus; G protein; Glycoprotein.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600002
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