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Registros recuperados: 79
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An optmized protocol for simultaneous extraction of DNA and RNA from soils BJM
Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia.
In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011
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Análise da diversidade genética da população de Paenibacillus na rizosfera de milho plantado em solo de cerrado e de várzea, através de métodos moleculares. Repositório Alice
SILVA, K.R.; ROSADO, A.S.; MARRIEL, I. E.; PAIVA, E.; SELDIN, L..
1999
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Milho; Rizosfera; Cerrado; Varzea; Diversidade genetica; Zea mays; Paenibacillus; Maize; Rhizosphere; Genetic diversity; Savanna.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483538
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Aspergillus niveus Blochwitz 4128URM: new source for inulinase production BABT
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de.
Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
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Assessing the diversity of bacterial communities associated with plants BJM
Andreote,Fernando Dini; Azevedo,João Lúcio; Araújo,Welington Luiz.
Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant-bacteria interactions; Molecular techniques; Multivariate analysis; Endophytes; Rhizosphere.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300001
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Avaliação do pH da rizosfera em dois genótipos de sorgo, em três saturações de alumínio e três níveis de potássio. Repositório Alice
PITTA, G.V.E.; BALIGAR, V.C.; CLARK, R.B.; RITCHIE, K.D.; ALVES, V. M. C.; VASCONCELLOS, C.A..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sorgo; PH; Rizosfera; Aluminio; Solo acido; Potassio; Sorghum bicolor; Acid soils; Aluminium; Potassium; Rhizosphere.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484409
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Back to the roots: revealing beneficial interactions between the rhizosphere microbiome and wild wheat. Repositório Alice
ROSSMANN, M.; PÉREZ-JARAMILLO, J. E.; CHIARAMONTE, J. B.; KAVAMURA, V. N.; DUMACK, K.; FIORE-DONNO, A. M.; MENDES, L. W.; BONKOWSKI, M.; MAUCHLINE, T.; RAAIJMAKERS, J. M.; MENDES, R..
Domestication of plants may have impacted the interactions between plants and their rhizosphere microbiome. Considering that plants rely on the rhizosphere microbiome for functions related with plant growth, nutrition and health, the objective of this work was to analyse the rhizosphere microbiome of wild relatives and modern varieties of wheat and to investigate whether wild relatives recruit a higher proportion of beneficial microbes than modern cultivars. By 16S-rRNA gene amplicon, ITS and 18SrRNA gene amplicon, we assessed the bacterial, fungal and cercozoa rhizosphere community composition of five modern cultivars and three wheat ancestors cultivated in forest and agricultural soils. Results revealed a pronounced soil type and rhizosphere effect on...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Wild wheat; Trigo; Rizosfera; Rhizosphere; Wheat; Microbiome.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103217
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Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis Phyton
Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G.
Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gamma­proteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012
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Bacterial diversity in the rhizosphere of a transgenic versus a conventional maize (Zea mays) Phyton
Vital-López,L; Cruz-Hernández,MA; Fernández-Dávila,S; Mendoza-Herrera,A.
Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Glufosinate; Metagenomic DNA; Rhizosphere; Single-strand conformation polymorphism.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200006
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Biological nitrogen fixation and agronomic features of soybean (Glycine max (L.)Merr.) crop under different doses of inoculant Acta Agron. (Palmira)
Pedrozo,Alessandro; Girelli de Oliveira,Nelson João; Alberton,Odair.
Abstract Soybean is one of the most important crops in the world and presents great versatility. This study aims to investigate the effect of the Bradyrhizobium japonicum inoculant at 0, 600 and 1200 mL ha1 on soybean crop, with and without 1 L ha-1 calcium (Ca 41%) + 0.4 L ha-1 boron (B 14%). Plants were grown for 90 days in plastic pots with 3 kg of sand and vermiculite (v/v) autoclaved at 121oC for 1 h. The experiment was conducted in a completely randomized design with five replications in a greenhouse. Soybean yield parameters were evaluated, such as, dry weight of shoot, root and total; plant height, nitrogen in the shoot; number and dry weight of nodules; chlorophyll index in the leaves; number of pods per plant (NPPL); number of grains per pod...
Tipo: Journal article Palavras-chave: Bradyrhizobium; Inoculation; Plant-soil relations; Rhizosphere; Soil microbiology; Symbiosis.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200297
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Breeding for resistance to soil-borne pathogen impacts rhizosphere microbiome functions in common bean. Repositório Alice
MENDES, L. W.; MENDES, R.; RAAIJMAKERS, J. M.; TSAI, S. M..
Over the past century, plant breeding programs have substantially improved plant growth and health, but have not yet considered the potential effects on the plant microbiome. Here, we conducted a metatranscriptome analysis to determine if and how breeding for resistance of common bean against the root pathogen Fusarium oxysporum (Fox) affected gene expression in the rhizobacterial community. Our data revealed that rhizobacterial community assembly of Fox-resistant and susceptible bean cultivars follows niche-based mechanisms, presenting lower diversity and distinct community structure compared to the bulk soil. The microbiome of the Fox-resistant bean cultivar presented a significantly higher expression of bacterial genes associated with nutrient...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Rizosfera; Feijão; Microbiome; Rhizosphere; Beans; Breeding.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103238
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Characterization of Rhizobacteria from field grown Genetically Modified (GM) and non-GM maizes BABT
Bumunang,Emmanuel Wihkochombom; Babalola,Olubukola Oluranti.
This study was done to examine the rhizobacteria from field grown Genetically Modified (GM) maize and its non-GM counterpart. Rhizospheric soil samples were collected at 30 days after sowing (DAS) and at post-harvest from two experimental fields in Gauteng, South Africa. Total rhizobacteria (cfu/g) in GM and non-GM soil samples was not significantly different across the different media 30 DAS and at post-harvest. Rhizobacterial isolates obtained were biochemically characterized using the analytical profile index. Species of Pseudomonas, Aeromonas, Sphingomonas, Burkholderia, Stenotrophomonas, Achromobacter, Ewingella and Bacillus were screened in vitro for plant growth promoting traits such as, ammonia production, catalase activity, indole acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetically modified maize; Plant growth promoting traits; Rhizosphere; Rhizobacteria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100001
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Combined application of bio-organic phosphate and phosphorus solubilizing bacteria (Bacillus strain MWT 14) improve the performance of bread wheat with low fertilizer input under an arid climate BJM
Tahir,Muhammad; Khalid,Umaira; Ijaz,Muhammad; Shah,Ghulam Mustafa; Naeem,Muhammad Asif; Shahid,Muhammad; Mahmood,Khalid; Ahmad,Naveed; Kareem,Fazal.
Abstract This study was aimed to investigate the effect of bio-organic phosphate either alone or in combination with phosphorus solubilizing bacteria strain (Bacillus MWT-14) on the growth and productivity of two wheat cultivars (Galaxy-2013 and Punjab-2011) along with recommended (150–100 NP kg ha−1) and half dose (75–50 NP kg ha−1) of fertilizers. The combined application of bio-organic phosphate and the phosphorous solubilizing bacteria strain at either fertilizer level significantly improved the growth, yield parameters and productivity of both wheat cultivars compared to non-inoculated control treatments. The cultivar Punjab-2011 produced the higher chlorophyll contents, crop growth rate, and the straw yield at half dose of NP fertilizer; while...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphorous; Phosphorous solubilizing bacteria; Bioavailability; Rhizosphere; Low fertilizer input.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500015
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Comparison of Paenibacillus azotofixans strains isolated from rhizoplane, rhizosphere, and non-root-associated soil from maize planted in two different Brazilian soils. Repositório Alice
SELDIN, L.; ROSADO, A. S.; CRUZ, D. W. da; NOBREGA, A.; ELSAS, J. D. van; PAIVA, E..
Paenibacillus azotofixans is a nitrogen-fixing bacterium often found in soil and in the rhizospheres of different grasses. In this study, two Brazilian clay soils were planted with cross-hybrid maize (BR-201) and four stages of plant growth were analysed to characterize the P. azotofixans populations present in the rhizoplanes, rhizospheres, and non-root-associated soils (herein called nonrhizospheres). A total of 106 strains were isolated and identified as P. azotofixans with an API 50CH kit, by classical biochemical tests, and via the use of specific primers based on the 16S rRNA gene in PCRs. To compare the isolated strains, phenotypic characteristics were determined and three different probes were used in hybridization experiments: two nif probes and...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Maize; Soil types; Cerrado soils; Clay soils; Rhizosphere.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/489147
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Composition of arbuscular mycorrhizal fungi communities signals generalist species in soils cultivated with strawberry. Repositório Alice
CHIOMENTO, J. L. T.; STRÜRMER, S. L.; CARRENHO, R.; COSTA, R. C. da.; SCHEFFER-BASSO, S. M.; ANTUNES, L. E. C.; NIENOWA, A. A.; CALVETE, E. O..
bitstream/item/197053/1/Luis-Eduardo-Composition-of-arbuscular-mycorrhizal.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fragaria X ananassaduch Duch; Diversity; Natural ecosystems; Agricultural ecosystems; Rhizosphere.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108828
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Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum) Phyton
Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G.
Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenomics; Rhizosphere; Wheat.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018
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Cross-kingdom similarities in microbiome ecology and biocontrol of pathogens. Repositório Alice
BERG, G.; KRAUSE, R.; MENDES, R..
Abstract: The concept of "gut and root microbiota commonalities" was already presented by Ramírez-Puebla et al.(2013); they discussed a lot of similar functional traits, host-bacteria interactions as well as evolutionary trends but also several differences. Based on deeper insights obtained by omics technologies, Mendes and Raaijmakers (2015) recently presented their concept that the structure and function of rhizosphere and gut microbiomes showcross-kingdom similarities. In parallel, Hacquard et al. (2015) analyzed similarities of the microbiota composition across plant and animal kingdoms and found only little overlap comparing fish gut and plant root communities. They explained the differences by various start inoculants and abiotic, niche-specific...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ecological theories; Rizosfera; População microbiana; Bactéria; Controle biológico; Microbiome; Biological control agents; Rhizosphere; Pathogens.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032834
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Deciphering rhizosphere microbiome assembly of wild and modern common bean (Phaseolus vulgaris) in native and agricultural soils from Colombia. Repositório Alice
PEREZ-JARAMILLO, J. E.; HOLLANDER, M. de; RAMÍREZ, C. A.; MENDES, R.; RAAIJMAKERS, J. M.; CARRIÓN, V. J..
Abstract: Background: Modern crop varieties are typically cultivated in agriculturally well-managed soils far from the centers oforigin of their wild relatives. How this habitat expansion impacted plant microbiome assembly is not well understood. Results: Here, we investigated if the transition from a native to an agricultural soil affected rhizobacterial communityassembly of wild and modern common bean (Phaseolus vulgaris) and if this led to a depletion of rhizobacterialdiversity. The impact of the bean genotype on rhizobacterial assembly was more prominent in the agriculturalsoil than in the native soil. Although only 113 operational taxonomic units (OTUs) out of a total of 15,925 wereshared by all eight bean accessions grown in native and agricultural...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Common bean; Wild and modern accessions; Domestication; Rhizosphere; Core microbiome; Networks.; Feijão; Rizosfera; Phaseolus; Microbiome; Rhizosphere; Domestication.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115028
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Disentangling the influence of earthworms in sugarcane rhizosphere. Repositório Alice
BRAGA, L. P. P.; YOSHIURA, C. A.; BORGES, C. D.; HORN, M. A.; BROWN, G. G.; DRAKE, H. L.; TSAI, S. M..
For the last 150 years many studies have shown the importance of earthworms for plant growth, but the exact mechanisms involved in the process are still poorly understood. Many important functions required for plant growth can be performed by soil microbes in the rhizosphere. To investigate earthworm influence on the rhizosphere microbial community, we performed a macrocosm experiment with and without Pontoscolex corethrurus (EW+ and EW?, respectively) and followed various soil and rhizosphere processes for 217 days with sugarcane. In EW+ treatments, N2O concentrations belowground (15 cm depth) and relative abundances of nitrous oxide genes (nosZ) were higher in bulk soil and rhizosphere, suggesting that soil microbes were able to consume earthworm-induced...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Crescimento de plantas; Minhoca; Solo; Rizosfera; Biologia do solo; Earthworms; Rhizosphere.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1067637
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Disponibilização de K de rochas silicáticas para plantas de soja inoculadas com fungos micorrízicos arbusculares (FMA). Infoteca-e
ANDRADE, L. R. M. de; FALEIRO, A. S. G.; SANTOS SOBRINHO, D. A.; NASCIMENTO, M. T. DO; SOUZA, A. L. DE.
2011
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Soja; Fungo micorrízico arbuscular; Rizosfera; Nutrientes; Soybeans; Arbuscular mycorrhizal fungus; Rhizosphere; Nutrients.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/931541
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Diversidade metabólica de microrganismos em rizosfera de linhagens e híbridos de milho contrastantes para fósforo - campo. Repositório Alice
OLIVEIRA, C.A. de; MARRIEL, I. E.; ALVES, V. M. C.; PARENTONI, S. N.; PEREIRA FILHO, I. A.; SCHAFFERT, R. E.; MUZZI, M.R.S.; SA, N.M.H. de.
A avaliacao da diversidade microbiana e um bom indicador da qualidade dos solos e de diferencas existentes entre solos de rizosfera de plantas. O objetivo desse trabalho foi avaliar a diversidade metabolica de microrganismos totais da rizosfera, pelo sistema BIOLOG. O solos rizosferico de genotipos de milho, eficientes (E) e ineficientes (I) para fosforo, em alta e baixo P foram coletados na Embrapa Milho e Sorgo, Sete Lagoas, MG, Os hibridos avaliados foram: HT, HS1, HS2, eficientes e L22, ineficientes. Como amostras testemunhas: cerrado natural (mata), plantio direto e solo nao rizosferico dos hibridos e linhagens. As comunidades microbianas tivera, perfil distinto de utilizacao das fontes de carbono, conforme a analise de agrupamento e PCA. Amostras de...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Rizosfera; Diversidade; Microorganismos; Fosforo; Cerrado; Zea mays; Maize; Rhizosphere; Diversity; Microorganisms; Phosphorus; Savanna.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/474378
Registros recuperados: 79
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