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Registros recuperados: 35
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An optmized protocol for simultaneous extraction of DNA and RNA from soils BJM
Costa,Rodrigo; Gomes,Newton C.M.; Milling,Annett; Smalla,Kornelia.
In this work we report an optimized protocol for simultaneous extraction of DNA and RNA from soil matrices. Treatment of soil matrices with ethanol followed by bead-beating worked as a successful strategy to lyse the cells without considerable degradation of nucleic acids, resulting in DNA and RNA of good yield and integrity. The reverse transcribed RNA could be amplified with primers targeting a glutamine synthetase (glnA) gene fragment. From both DNA and cDNA, 16S rDNA fragments were amplified and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). The method was applied to soil and rhizosphere (strawberry and oilseed rape) samples. Two other protocols for the extraction of nucleic acids from soil were applied to the same set of samples in order...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNA extraction; Nucleic acids; Rhizosphere; Microbial communities.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200011
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Aspergillus niveus Blochwitz 4128URM: new source for inulinase production BABT
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Porto,Ana Lúcia Figueiredo; Moreira,Keila Aparecida; Lima Filho,José Luiz de.
Aspergillus niveus Blochwitz 4128 URM isolated from sunflower rhizosphere demonstrated a new source of inulinase. The enzyme was produced in culture medium containing inulin as substrate in the concentrations: 10, 15 and 20g L-1. Maximum enzyme activity was obtained in medium containing 20g L-1 inulin. The enzyme was partially purified using ammonium sulphate precipitation, followed by ion charge (DE-32) and molecular exclusion (Sephadex) chromatography. The results showed the optimal pH and temperature of inulinase from crude extract were 4.0 and 4.8 and 45ºC, respectively. The enzyme was purified 34.65 fold with yield of 53.63%. A. niveus 4128URM can be used in the inulinase production with use in the food industries.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niveus; Rhizosphere; Inulinase; Inulin; Purification.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300003
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Assessing the diversity of bacterial communities associated with plants BJM
Andreote,Fernando Dini; Azevedo,João Lúcio; Araújo,Welington Luiz.
Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant-bacteria interactions; Molecular techniques; Multivariate analysis; Endophytes; Rhizosphere.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300001
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Bacterial diversity associated with the rhizosphere of wheat plants (Triticum aestivum): Toward a metagenomic analysis Phyton
Velázquez-Sepúlveda,I; Orozco-Mosqueda,MC; Prieto-Barajas,CM; Santoyo,G.
Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gamma­proteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Rhizosphere; Wheat; 16S ribosomal genes.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100012
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Bacterial diversity in the rhizosphere of a transgenic versus a conventional maize (Zea mays) Phyton
Vital-López,L; Cruz-Hernández,MA; Fernández-Dávila,S; Mendoza-Herrera,A.
Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Glufosinate; Metagenomic DNA; Rhizosphere; Single-strand conformation polymorphism.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200006
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Biological nitrogen fixation and agronomic features of soybean (Glycine max (L.)Merr.) crop under different doses of inoculant Acta Agron. (Palmira)
Pedrozo,Alessandro; Girelli de Oliveira,Nelson João; Alberton,Odair.
Abstract Soybean is one of the most important crops in the world and presents great versatility. This study aims to investigate the effect of the Bradyrhizobium japonicum inoculant at 0, 600 and 1200 mL ha1 on soybean crop, with and without 1 L ha-1 calcium (Ca 41%) + 0.4 L ha-1 boron (B 14%). Plants were grown for 90 days in plastic pots with 3 kg of sand and vermiculite (v/v) autoclaved at 121oC for 1 h. The experiment was conducted in a completely randomized design with five replications in a greenhouse. Soybean yield parameters were evaluated, such as, dry weight of shoot, root and total; plant height, nitrogen in the shoot; number and dry weight of nodules; chlorophyll index in the leaves; number of pods per plant (NPPL); number of grains per pod...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradyrhizobium; Inoculation; Plant-soil relations; Rhizosphere; Soil microbiology; Symbiosis.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200297
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Characterization of Rhizobacteria from field grown Genetically Modified (GM) and non-GM maizes BABT
Bumunang,Emmanuel Wihkochombom; Babalola,Olubukola Oluranti.
This study was done to examine the rhizobacteria from field grown Genetically Modified (GM) maize and its non-GM counterpart. Rhizospheric soil samples were collected at 30 days after sowing (DAS) and at post-harvest from two experimental fields in Gauteng, South Africa. Total rhizobacteria (cfu/g) in GM and non-GM soil samples was not significantly different across the different media 30 DAS and at post-harvest. Rhizobacterial isolates obtained were biochemically characterized using the analytical profile index. Species of Pseudomonas, Aeromonas, Sphingomonas, Burkholderia, Stenotrophomonas, Achromobacter, Ewingella and Bacillus were screened in vitro for plant growth promoting traits such as, ammonia production, catalase activity, indole acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetically modified maize; Plant growth promoting traits; Rhizosphere; Rhizobacteria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100001
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Combined application of bio-organic phosphate and phosphorus solubilizing bacteria (Bacillus strain MWT 14) improve the performance of bread wheat with low fertilizer input under an arid climate BJM
Tahir,Muhammad; Khalid,Umaira; Ijaz,Muhammad; Shah,Ghulam Mustafa; Naeem,Muhammad Asif; Shahid,Muhammad; Mahmood,Khalid; Ahmad,Naveed; Kareem,Fazal.
Abstract This study was aimed to investigate the effect of bio-organic phosphate either alone or in combination with phosphorus solubilizing bacteria strain (Bacillus MWT-14) on the growth and productivity of two wheat cultivars (Galaxy-2013 and Punjab-2011) along with recommended (150–100 NP kg ha−1) and half dose (75–50 NP kg ha−1) of fertilizers. The combined application of bio-organic phosphate and the phosphorous solubilizing bacteria strain at either fertilizer level significantly improved the growth, yield parameters and productivity of both wheat cultivars compared to non-inoculated control treatments. The cultivar Punjab-2011 produced the higher chlorophyll contents, crop growth rate, and the straw yield at half dose of NP fertilizer; while...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphorous; Phosphorous solubilizing bacteria; Bioavailability; Rhizosphere; Low fertilizer input.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500015
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Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum) Phyton
Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G.
Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenomics; Rhizosphere; Wheat.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018
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Disponibilização de K de rochas silicáticas para plantas de soja inoculadas com fungos micorrízicos arbusculares (FMA). Infoteca-e
ANDRADE, L. R. M. de; FALEIRO, A. S. G.; SANTOS SOBRINHO, D. A.; NASCIMENTO, M. T. DO; SOUZA, A. L. DE.
bitstream/item/75813/1/bolpd-303.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Fungo micorrízico arbuscular; Nutrientes; Arbuscular mycorrhizal fungus.; Rizosfera; Soja.; Nutrients; Soybeans.; Rhizosphere.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/931541
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Effect of Bacillus thuringiensis on microbial functional groups in sorghum rhizosphere PAB
Brasil,Carlos; Matsumoto,Leopoldo Sussumu; Nogueira,Marco Antonio; Spago,Flavia Regina; Rampazo,Luís Gustavo; Cruz,Marcio Ferreira; Andrade,Galdino.
The objective of this work was to assess the effect of two strains of Bacillus thuringiensis var. kurstaki on sorghum rhizosphere microorganisms. The strains were HD1, that produces the bioinsecticidal protein, and 407, that is a mutant non-producer. The strains do not influence microbial population, but reduce plant growth and improve mycorrhizal colonization and free living fixing N2 community.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bioinsecticide; Sorgum; Rhizosphere; Biocontrol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000500022
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Effect of plant photosynthesis, carbon sources and ammonium availability on nitrogen fixation rates in the rhizosphere of Zostera noltii ArchiMer
Welsh, Dt; Bourgues, S; Dewit, R; Auby, Isabelle.
Rates of nitrogen fixation (measured as acetylene reduction) in the rhizosphere of the seagrass Zostera noltii were highly dependent upon plant photosynthetic activity being significantly stimulated at elevated CO2 concentrations and by light, both in the short-term and over diurnal cycles. Stimulation by light became insignificant when 5 mM sucrose was added to the sediment porewater, indicating that in the absence of added carbon sources, light stimulation was due to direct inputs of plant photosynthate to the rhizosphere. Addition of a range of carbon sources to the rhizosphere sediment stimulated rates of acetylene reduction, with this stimulation being significant for sucrose and lactate. Surprisingly, whilst low additions of ammonium to the sediment...
Tipo: Text Palavras-chave: Acetylene reduction; Nitrogen fixation; Rhizosphere; Zostera noltii; Plant photosynthesis; Root exudates; Carbon sources; Ammonium.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00151/26266/24350.pdf
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Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile Ciencia e Investigación Agraria
Cáceres,Pablo; Cordero,Cecilia; González,Gloria; Quiroz,Karla; Bobadilla,Juan C; Bravo,Carmen; Caligari,Peter D.S; Carrasco,Basilio; García-Gonzales,Rolando.
A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use in biodiversity studies. Similarly, the effect of liquid nitrogen on the process of cell disruption in all of the protocols that were studied. DNA yields ranged from 12.4 ng g-1 of processed soil to 9620 ng g-1 using the modified lysis buffer and commercial extraction kit, respectively. The quality of the DNA was determined by the ability of the DNA to produce efficient and reproducible polymerase chain reaction (PCR) products, using primers for universal 16S...
Tipo: Journal article Palavras-chave: Hydrophilic forests; 16S ribosomal RNA; PCR; Rhizosphere; Soil DNA extraction.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018
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Enhancement of clover growth by inoculation of P-solubilizing fungi and arbuscular mycorrhizal fungi Anais da ABC (AABC)
Souchie,Edson L.; Azcón,Rosario; Barea,Jose M.; Silva,Eliane M.R.; Saggin-Júnior,Orivaldo J..
This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araxá apatite; Phosphate solubilization; Co-inoculation; Plant growth promotion; Rhizosphere.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000300023
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Exploring interactions of plant microbiomes Scientia Agricola
Andreote,Fernando Dini; Gumiere,Thiago; Durrer,Ademir.
A plethora of microbial cells is present in every gram of soil, and microbes are found extensively in plant and animal tissues. The mechanisms governed by microorganisms in the regulation of physiological processes of their hosts have been extensively studied in the light of recent findings on microbiomes. In plants, the components of these microbiomes may form distinct communities, such as those inhabiting the plant rhizosphere, the endosphere and the phyllosphere. In each of these niches, the "microbial tissue" is established by, and responds to, specific selective pressures. Although there is no clear picture of the overall role of the plant microbiome, there is substantial evidence that these communities are involved in disease control, enhance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial communities; Rhizosphere; Endophytes; Phyllosphere.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000600013
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Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis Electron. J. Biotechnol.
Kumar Gothwal,Raj; Kumar Nigam,Vinod; Mohan,M. Krishna; Sasmal,Dinakar; Ghosh,Purnendu.
A reliable method for characterizing microbial communities on the basis of their differences in the 16S ribosomal RNA (rRNA) gene sequences in the hot arid zone sandy soils has been optimized. A desert plant (Calligonum polygonoides) was chosen to provide the rhizospheric soil samples, collected from three different agro-ecological locations. Total community DNA was efficiently extracted at small-scale level using direct lysis with hot sodium dodecyl sulphate (SDS), glass bead beating and finally subjecting the sandy soil to liquid nitrogen freeze-thaw cycles. To amplify V3 region of bacterial 16S rRNA gene, universal conserved primers were used. Second round polymerase chain reaction (PCR) was attempted to increase product concentration and to minimize...
Tipo: Journal article Palavras-chave: Arid soils; Microbial community; PCR-DGGE; Rhizosphere; Soil DNA extraction.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300007
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Fluorescent Pseudomonads associated with the rhizosphere of crops: an overview BJM
Botelho,Gloria R.; Mendonça-Hagler,Leda Cristina.
Environmental concerns have led to the need of sustainable use of natural resources. The conventional agriculture caused considerable impacts on soils and waters. It is important to change certain agricultural managements to environmental cleaner techniques. The sustainable agriculture has pointed many approaches and techniques to reduce environmental impact. One of those strategies is the utilization of the soil microbiota to induce plant growth, control of plant diseases and biodegradation of xenobiotic compounds. Studies on the relationship between roots and microbiota are essential to achieve viable agricultural applications. These studies indicate that one of the most abundant microorganisms in the rhizosphere (area around the roots) is fluorescent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth promoting; Biological control; Fluorescent Pseudomonads; Rhizosphere.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400001
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Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin BJM
Souza-Motta,Cristina Maria de; Cavalcanti,Maria Auxiliadora de Queiroz; Fernandes,Maria José dos Santos; Lima,Débora Maria Massa; Nascimento,João Paulo; Laranjeira,Delson.
Filamentous fungi able to hydrolyse inulin have been isolated from the rhizosphere of plants whose roots contain this polysaccharide. This study reports results concerning the isolation and identification of filamentous fungi from the soil used for sunflower cultivation and from the sunflower rhizosphere cultivated in field and in greenhouse. Fungi were evaluated according to their capacity to hydrolyse inulin and the variation in the diversity of these fungi during the plant's life cycle was also accessed. Forty-nine species of filamentous fungi were isolated. Penicillium and Aspergillus were the genera that presented higher number of species, nine and seven, respectively. At the end of the sunflower life cycle, cultivated both in field and in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Rhizosphere; Soil; Inulinase; Sunflower.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300018
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Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing BJM
Naveed,Muhammad; Mubeen,Samavia; khan,SamiUllah; Ahmed,Iftikhar; Khalid,Nauman; Suleria,Hafiz Ansar Rasul; Bano,Asghari; Mumtaz,Abdul Samad.
In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Root nodules; 16S rRNA gene sequence; Glucose dehydrogenase; Pyrroloquinolone quinone.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300031
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Interaction between Thermotolerant Coliforms and Rhizobacteria in Soil Fertilized with Treated Domestic Wastewater Rev. Bras. Ciênc. Solo
Fortes Neto,Paulo; Fortes,Nara Lúcia Perondi; Silva,Eliana Maria de Araújo Mariano da; Brambatti,Fabiana.
ABSTRACT Studies on the survival of pathogenic microorganisms in the soil after use of wastewater for fertilization of agricultural crops report the effects of moisture, pH, organic matter, and soil temperature on microorganisms. There are few studies that assess the survival of these microorganisms in the rhizosphere of plants fertilized with wastewater. Thus, the aim of this study was to quantify the number of fecal coliforms and rhizobacteria (fluorescent Pseudomonas spp., Bacillus spp,) in the rhizosphere of winter and summer crops fertilized with wastewater. In the experiment, we used 20 plots, and each plot occupied an area of 200 m2. The treatments used in the winter crop consisted of uncultivated plots and single crops of wheat, triticale, black...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Reclaimed water; Wastewater.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100414
Registros recuperados: 35
Primeira ... 12 ... Última
 

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