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| Registros recuperados: 12 | |
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| Mercey, R.; Lantier, I.; Maurel, M.C.; Grosclaude, J.; Lantier, F.; Marc, D.. |
| One of the unsolved problems in prion diseases relates to the physiological function of cellular prion protein (PrP), of which a misfolded isoform is the major component of the transmissible spongiform encephalopathies agent. Knowledge of the PrP-binding molecules may help in elucidating its role and understanding the pathological events underlying prion diseases. Because nucleic acids are known to bind PrP, we attempted to identify the preferred RNA sequences that bind to the ovine recombinant PrP. An in vitro selection approach (SELEX) was applied to a pool of 80-nucleotide(nt)-long RNAs containing a randomised 40-nt central region. The most frequently isolated aptamer, RM312, was also the best ligand (20 nM KD value), according to both surface plasmon... |
| Tipo: Journal Article |
Palavras-chave: SEQUENCE; AMPLIFICATION; TRANSCRIPTION. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007b7bf6e45&uri=/notices/prodinra1/2010/10/ |
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| Grimplet, J.; Gaspar, J.W.; Gancel, A.L.; Sauvage, F.X.; Romieu, C.. |
| In order to improve protein assignment from peptide mass fingerprints (PMF) in species with incompletely sequenced genomes, the genus-specific mutations deduced from Expressed sequence tag (EST) sequences were included in the complete reading frames of orthologous proteins, resulting in a new searchable in silico protein database. Using this method in tests on four plant species, the MOWSE score of at least 20% more proteins was improved compared to conventional approaches on crude, total proteins, for middle-sized EST projects. Larger contigs are assembled in more important EST projects and this improves the conventional assignment of the most abundant proteins. However, contigs from minor transcripts remain shorter and the assignment of less abundant... |
| Tipo: Journal Article |
Palavras-chave: IDENTIFICATION; PROTEINE; SEQUENCE; GRAPPE DE RAISIN; CITRON ANNOTATION; EXPRESSED SEQUENCE TAG; NON MODEL GENOME; PEPTIDE MASS FINGER-PRINTING. |
| Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200799d3dd0b&uri=/notices/prodinra1/2007/02/ |
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| Dequin, S.. |
| The end of the twentieth century was marked by major advances in life technology, particularly in areas related to genetics and more recently genomics. Considerable progress was made in the development of genetically improved yeast strains for the wine, brewing and baking industries. In the last decade, recombinant DNA technology widened the possibilities for introducing new properties. The most remarkable advances, which are discussed in this Mini-Review, are improved process performance, off-flavor elimination, increased formation of by-products, improved hygienic properties or extension of substrate utilization. Although the introduction of this technology into traditional industries is currently limited by public perception, the number of... |
| Tipo: Journal Article |
Palavras-chave: GLUCOSE; SACCHAROMYCES CERIVISIAE; SEQUENCE; OENOLOGIE. |
| Ano: 2001 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025011103344&uri=/notices/prodinra1/2010/09/ |
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| Registros recuperados: 12 | |
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