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A new approach for obtaining rapid uniformity in rice (Oryza sativa L.) via a 3x x 2x cross Genet. Mol. Biol.
Xing,Shaochen; Cai,Yuhong; Zhou,Kaida.
A triploid (2n = 3x = 36) rice plant was obtained by screening a twin seedling population in which each seed germinated to two or three sprouts that were then crossed with diploid plants. One diploid plant was chosen among the various F1 progenies and developed into an F2 population via self-pollination. Compared with the control variety Shanyou 63, this F2 population had a stable agronomical performance in field trials, as confirmed by the F-test. The stability of the F2 population was further substantiated by molecular analysis with simple sequence repeat markers. Specifically, of 160 markers assayed, 37 (covering all 12 chromosomes) were polymorphic between the parental lines. Testing the F1 hybrid individually with these markers showed that each PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: F-test; Polyploidy; Rice; SSR marker; Stability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200020
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Molecular markers useful to discriminate Coffea arabica cultivars with high genetic similarity. Repositório Alice
SOUSA, T. V.; CAIXETA, E. T.; ALKIMIM, E. R.; OLIVEIRA, A. C. B. de; PEREIRA, A. A.; ZAMBOLIM, L.; SAKIYAMA, N. S..
New cultivars are released every year to meet market demands. However, in species with a narrow genetic base, such as Coffea arabica, the cultivars are closely related and phenotypically similar. This hinders the accurate discrimination of genotypes sing morphological descriptors in distinctness, uniformity, and stability (DUS) testing, which is required for the registration and protection of new cultivars. In this sense, molecular markers are an auxiliary tool for accurate and precise discrimination of cultivars. This study aimed to verify the informative capacity and effectiveness of a molecular marker set to discriminate among C. arabica varieties, create a database of DNA profiles and allele frequencies, analyze the genetic diversity in this...
Tipo: Artigo de periódico Palavras-chave: Molecular profiling; Protection of plant cultivars; Cultivars register; SSR marker; Coffea; DNA fingerprinting; Genetic variation; Cultivar identification.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1067171
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Unraveling candidate genes underlying biomass digestibility in elephant grass (Cenchrus purpureus). Repositório Alice
ROCHA, J. R. do A. S. de C.; MARÇAL, T. de S.; SALVADOR, F. V.; SILVA, A. C. da; CARNEIRO, P. C. S.; RESENDE, M. D. V. de; CARNEIRO, J. da C.; AZEVEDO, A. L. S.; PEREIRA, J. F.; MACHADO, J. C..
Background: Elephant grass [Cenchrus purpureus (Schumach.) Morrone] is used for bioenergy and animal feed. In order to identify candidate genes that could be exploited for marker-assisted selection in elephant grass, this study aimed to investigate changes in predictive accuracy using genomic relationship information and simple sequence repeats for eight traits (height, green biomass, dry biomass, acid and neutral detergent fiber, lignin content, biomass digestibility, and dry matter concentration) linked to bioenergetics and animal feeding. Results: We used single-step, genome-based best linear unbiased prediction and genome association methods to investigate changes in predictive accuracy and find candidate genes using genomic relationship information....
Tipo: Artigo de periódico Palavras-chave: Gene annotation; Napier grass; SSR marker; Trait-marker association; Pennisetum Purpureum; Capim Elefante; Biomassa; Digestibilidade.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116956
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