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A simple and rapid method for collecting saliva to assess sodium status in sheepum Ciência Rural
Ortolani,Enrico Lippi.
Fifteen lambs were fed diet with normal (0.11%) or deficient levels of sodium ( 0.035%), respectively, for five weeks on each diet. At the end of each period saliva was collected by three different methods: swabbing with a 5 x 5 x 1.25cm synthetic sponge, aspiration with a 15cm long plastic tube connected to a 20ml syringe and sucking with a 4mm x 45cm long plastic tube through a water-jet aspirator pump or an eletric motor pump. The volume of saliva collected/min and the salivary sodium: potassium ratio were analysed. Larger volume of saliva was obtained with the developed sucking apparatus than with the sponge or the syringe. There was no influence of the method of saliva collection on the values of salivary Na:K ratio in both periods. Nevertheless, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saliva; Collection; Methods; Sheep; Salivary sodium: potassium ratio.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781997000200012
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Análise do CAEV em saliva e dinâmica viral em sêmen de reprodutores nas fases aguda e crônica da infecção. Repositório Alice
SOUZA, K. C. de..
Resumo: A detecção do material genético do vírus da artrite encefalite caprina (CAEV) em sêmen de machos recentemente infectados, pode ocorrer antes mesmo da resposta sorológica. Adicionalmente, a transmissão do vírus por inseminação artificial com sêmen infectado foi recentemente comprovada. Contudo, há necessidade de buscar maior conhecimento do que acontece neste fluido em todas as fases da infecção. Além do sêmen, deve-se atentar a presença do vírus em outras amostras como em saliva, que tem permitido o diagnóstico de doenças infecciosas em humanos e animais. Dessa forma, o primeiro objetivo deste estudo foi investigar a presença do DNA proviral do CAEV em amostras de saliva de reprodutores infectados. Para tanto dez machos caprinos divididos em dois...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Vírus da artrite encefalite caprina; CAEV; Infecção aguda; Infecção crônica; Doença crônica; Caprino; Saliva; Sêmen; Transmissão de doença; Goats; Infectious diseases; Acute course; Chronic course; Saliva; Semen; Caprine arthritis encephalit virus.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082451
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Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis BJM
Rosalem Junior,Wilson; Souza,Rodrigo Carvalho de; Andrade,Arnaldo Feitosa Braga de; Colombo,Ana Paula Vieira.
Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Generalized aggressive periodontitis; PCR; Actinobacillus actinomycetemcomitans; Saliva; Leukotoxin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006
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Characterization of mouse salivary polypeptide secretion after oral administration of pilocarpine RChHN
LÓPEZ-SOLÍS,REMIGIO; PUENTE,MARGARITA; DURÁN,VIVIANA; MORALES-BOZO,IRENE; KEMMERLING,ULRIKE; PARDO,RODRIGO; WENK,CAROLINA.
Mouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the range 40-400 µg provoked a dose-dependent secretory response. The secretory response lasted for about 40 minutes and was constituted by four clearly defined stages, namely: lag (5-10 min), maximal flow (10 min), slowering (15-20 min) and postsecretory rest. In this response, usual parameters were: maximal flow rate, 30-40 µl/min; total volume of saliva, 250-350 µl/mouse and total salivary protein, about 700 µg/mouse. Temporal desensitization of the secretory...
Tipo: Journal article Palavras-chave: Mouse; Saliva; Secretion; Pilocarpine; Polypeptides.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023
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Contaminação salivar da extrusa em novilhos alimentados com diferentes volumosos, com e sem suplementação Arq. Bras. Med. Vet. Zootec.
Gomes,S.P.; Leão,M.I.; Valadares Filho,S.C.; Paulino,M.F..
Determinaram-se as alterações nos teores de proteína bruta (PB), compostos nitrogenados não protéicos (NNP), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), lignina (LIG), cinzas, cálcio (Ca), fósforo (P), magnésio (Mg), potássio (K) e sódio (Na) de alimentos usados para novilhos, provocadas pela contaminação da extrusa pela saliva. Foram utilizados cinco novilhos fistulados no rúmen e esôfago, cinco dietas e cinco períodos experimentais. Os tratamentos foram: capim-elefante picado (CE), capim-elefante obtido em pastejo simulado (CES), capim-braquiária obtido em pastejo simulado (CBS), pré-secado de capim-tifton 85 (PS), feno de capim-braquiária (FB1), feno de capim-tifton 85 (FT), feno de capim-braquiária e concentrado a 0,5% PV (FB2) e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Novilho; Contaminação da extrusa; Saliva; Volumoso; Concentrado.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600032
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Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. Repositório Alice
SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S..
[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: CAEV; PCR; Artrite-encefalite Caprina; Caprino; Doença animal; Vírus; Transmissão de doença; Disease transmission; Goats; Animal diseases; Caprine arthritis encephalit virus; PCR; Saliva; DNA sequence.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032574
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Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes J. Venom. Anim. Toxins incl. Trop. Dis.
Rasmussen,LT; Labio,RW de; C Neto,A; Silva,LC; Queiroz,VF; Smith,MAC; Payão,SLM.
Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter pylori; CagA; VacA; Dental plaque; Dyspeptic patient; Saliva.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200008
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Detection of hepatitis C virus RNA in saliva samples from patients with seric anti-HCV antibodies BJID
Gonçalves,Patrícia L.; Cunha,Carla B.; Busek,Solange C. U.; Oliveira,Guilherme C.; Ribeiro-Rodrigues,Rodrigo; Pereira,Fausto EL.
We examined the frequency of HCV-RNA in saliva samples from anti-HCV positive patients. Both plasma and saliva samples from 39 HCV patients (13 with normal liver enzymes, 19 with abnormal liver enzymes and 13 with cirrhosis) were investigated. Stimulated saliva and fresh plasma were centrifuged (900 x g,10 min) and stored at -70ºC, after the addition of guanidine isothiocyanate RNA extraction buffer. HCV-RNA was detected by RT- nested-PCR (amplification of HCV-cDNA for two rounds, using HCV primers 939/209 and 940/211). HCV genotyping was carried out by RFLP (using Mva I and Hinf 1 or Hae III and Rsa I restriction enzymes). Thirty-two out of 39 (82%; 95% CI=70-94%) anti-HCV-positive patients had HCV-RNA in plasma samples. Eight out of 39 (20.5%; 95%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HCV-RNA in saliva; Hepatitis C; Saliva.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100006
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Efeitos da saliva na composição química da forragem ingerida por bubalinos fistulado no esôfago. Repositório Alice
CAMARÃO, A. P.; DIAS, E..
1991
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Forragem; Saliva; Bubalino.
Ano: 1991 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1017401
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Haematophagous arthropod saliva and host defense system: a tale of tear and blood Anais da ABC (AABC)
Andrade,Bruno B.; Teixeira,Clarissa R.; Barral,Aldina; Barral-Netto,Manoel.
The saliva from blood-feeding arthropod vectors is enriched with molecules that display diverse functions that mediate a successful blood meal. They function not only as weapons against host's haemostatic, inflammatory and immune responses but also as important tools to pathogen establishment. Parasites, virus and bacteria taking advantage of vectors' armament have adapted to facilitate their entry in the host. Today, many salivary molecules have been identified and characterized as new targets to the development of future vaccines. Here we focus on current information on vector's saliva and the molecules responsible to modify host's hemostasis and immune response, also regarding their role in disease transmission.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saliva; Bites; Hemostasis; Host; Vector; Infection.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400008
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Measles, rubella, mumps and Toxoplasma gondii antibodies in saliva of vaccinated students of schools and universities in São Paulo City, Brazil BJID
Sampaio,Barbara Carvalho Fialho; Rodrigues,Jaqueline Polizeli; Meireles,Luciana Regina; Andrade Junior,Heitor Franco de.
ABSTRACT Introduction: Vaccines are well-established public health interventions with major impacton the prevalence of infectious diseases, but outbreaks are occurring frequently due to pri-mary and secondary failures, despite high coverage. Surveillance of efficacy and duration ofinduced immunity is a difficult task as it requires invasive blood sampling in children andteenagers. Saliva can be an acceptable alternative source of IgG to assess vaccine efficacyand toxoplasmosis incidence. We investigated IgG response for measles, mumps, rubella,and T. gondii in saliva samples of vaccinated young people. Methods: Saliva was collected from 249 public schools students from São Paulo, Brazil, aged7 to 13 years old, during an interactive exhibition on hygiene....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vaccine; Prevalence; Non-invasive; Saliva; IgG; Toxoplasmosis.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100051
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Minor Salivary Glands Morphology in Xerostomia Patients International Journal of Morphology
Liquidato,Bianca Maria; Barros,Mirna Duarte; Filho,Ivo Bussoloti; Pereira,Celina Siqueira Barbosa; Macéa,Maria Inêz Marcondes.
The minor salivary glands are found scattered throughout the oral mucosa, especially in the lips and soft palate mucosa. Several factors can cause xerostomia, whereas the salivary glands histological characteristics are also considered as factors for defining the etiology. Thus, the minor salivary glands biopsy represents an essential tool for attending the required diagnosis criterion in the classification of Sjögren's Syndrome patients, since it does not present risk for the patient. The objective of this study is to determine the histological description of the minor salivary glands obtained from the biopsies of xerostomia patients and to classify the minor salivary gland histological aspect as the Sjögren's Syndrome. Forty laminas of xerostomia...
Tipo: Journal article Palavras-chave: Sjögren's syndrome; Xerostomia; Salivary glands; Saliva; Diagnosis.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400031
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Mucocele salivar complexa em cão - relato de caso MV&Z
Santana, C. L.; Kowalesky, J.; Villamizar-Martinez, L. A.; Nascimento, N. A.; Fugita, M. S.; Gioso, M. A..
Mucocele consiste em um acúmulo de saliva no tecido subcutâneo adjacente a uma glândula ou ducto salivar. Raramente se identifica a causa da doença, no entanto alguns autores atribuem à origem causa traumática, corpo estranho, sialolitos. Este trabalho tem como objetivo apresentar um relato de mucocele complexa em cão, atendida no Laboratório de Odontologia Comparada (LOC) da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ – USP).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saliva; Glândula; Ducto; Mucocele.
Ano: 2014 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/24107
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Niveis de fosforo para caprinos: perda endogena fecal e exigencia liquida para mantenca. Repositório Alice
BUENO, M.S.; VITTI, D.M.S.S..
Foram utilizados, no experimento, nove caprinos machos castrados, da raca Alpina, em dois periodos. Os animais permaneceram 28 dias em dieta experimental composta de feno e concentrado, sem suplementacao de P, ou com 1g ou 2g de P, na forma de fosfato bicalcico. No 21 dia os animais receberam injecao de 7,4 MBq de 32P na jugular, e coletaram-se amostras de sangue, fezes e urina, por sete dias, com o objetivo de avaliar o metabolismo do P. O aumento do consumo de P levou a aumento linear significativo do P excretado nas fezes, do P absorvido, do P endogeno fecal, da eficiencia de absorcao, e dos teores de P nas fezes e no plasma. O aumento do P endogeno fecal (Y) em funcao do consumo de P (X) pode ser descrito pela equacao Y=10,36 + 0,58X (r=0,94); a perda...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fezes; Fosforo radioativo; Plasma; Saliva; Urina; Feces; Radioactive phsophorus; Urine.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/94150
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Níveis de fósforo para caprinos: perda endógena fecal e exigência líquida para mantença PAB
Bueno,Mauro Sartori; Vitti,Dorinha Miriam Silber Schmidt.
Foram utilizados, no experimento, nove caprinos machos castrados, da raça Alpina, em dois períodos. Os animais permaneceram 28 dias em dieta experimental composta de feno e concentrado, sem suplementação de P, ou com 1g ou 2 g de P, na forma de fosfato bicálcico. No 21º dia os animais receberam injeção de 7,4 MBq de 32P na jugular, e coletaram-se amostras de sangue, fezes e urina, por sete dias, com o objetivo de avaliar o metabolismo do P. O aumento do consumo de P levou a aumento linear significativo do P excretado nas fezes, do P absorvido, do P endógeno fecal, da eficiência de absorção, e dos teores de P nas fezes e no plasma. O aumento do P endógeno fecal (Y) em função do consumo de P (X) pode ser descrito pela equação Y= 10,36 + 0,58X (r=0,94); a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fezes; Fósforo radioativo; Plasma; Saliva; Urina.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999000400020
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Oral health, organic and inorganic saliva composition of men with Schizophrenia: case-control study. Repositório Alice
ROSA, L. K.; COSTA, F. S.; HAUAGGE, C. M.; MOBILE, R. Z.; LIMA, A. A. S. de; AMARAL, C. D. B. do; MACHADO, R. C.; NOGUEIRA, A. R. de A.; BRANCHER, J. A.; ARAUJO, M. R..
Background: Schizophrenia (SCZ) presents complex challenges related to diagnosis and clinical monitoring. The study of conditions associated with SCZ can be facilitated by using potential markers and patterns that provide information to support the diagnosis and oral health. Methods: The salivary composition of patients diagnosed with SCZ (n = 50) was evaluated and compared to the control (n = 50). Saliva samples from male patients were collected and clinical parameters were evaluated. The concentration of total proteins and amylase were determined and salivary macro- and microelements were quantified by ICP OES and ICP-MS. Exploratory data analysis based on artificial intelligence tools was used in the investigation. Results: There was a significant...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Self-organizing map; Exploratory data analysis; Saliva; Trace elements; Schizophrenia.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132841
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Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection BJM
Souto,Renata; Silva-Boghossian,Carina M.; Colombo,Ana Paula Vieira.
P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Acinetobacter spp; Subgingival biofilm; Saliva; Periodontitis; PCR.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200017
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Salivary testosterone and immunoglobulin A were increased by resistance training in adults with Down syndrome BJMBR
Fornieles,G.; Rosety,M.A.; Elosegui,S.; Rosety,J.M.; Alvero-Cruz,J.R.; Garcia,N.; Rosety,M.; Rodriguez-Pareja,T.; Toro,R.; Rosety-Rodriguez,M.; Ordonez,F.J.; Rosety,I..
This study was designed to assess the influence of resistance training on salivary immunoglobulin A (IgA) levels and hormone profile in sedentary adults with Down syndrome (DS). A total of 40 male adults with DS were recruited for the trial through different community support groups for people with intellectual disabilities. All participants had medical approval for participation in physical activity. Twenty-four adults were randomly assigned to perform resistance training in a circuit with six stations, 3 days per week for 12 weeks. Training intensity was based on functioning in the eight-repetition maximum (8RM) test for each exercise. The control group included 16 age-, gender-, and BMI-matched adults with DS. Salivary IgA, testosterone, and cortisol...
Palavras-chave: Down syndrome; Resistance training; Saliva; Immunoglobulin A; Testosterone; Cortisol.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400345
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