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| Nieddu,Mariella; Mezzanotte,Roberto; Pichiri,Giuseppina; Coni,Pier Paolo; Dedola,Gian Luca; Dettori,Maria Luisa; Pazzola,Michele; Vacca,Giuseppe Massimo; Robledo,Renato. |
| Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovidae; Satellite DNA; Fluorescence in situ hybridization; Pericentromeric region. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513 |
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| MONDIN, M.; SEREJO, J. A. dos S.; BERTÃO, M. R.; LABORDA, P.; PIZZAIA, D.; AGUIAR-PERECIN, M. L. R.. |
| Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic... |
| Tipo: Parte de livro |
Palavras-chave: Heterochromatic Knobs; Milho; DNA; Chromosomes; Satellite DNA; Centromeres. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097537 |
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| Kavalco,Karine Frehner; Pazza,Rubens; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando. |
| Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astyanax; Fluorescence in situ hybridization (FISH); Heterochromatin; Karyotypic diversity; Satellite DNA. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005 |
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