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Registros recuperados: 9
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Atividade fungitóxica de Momordica charantia L. no controle de Sclerotium rolfsii Sacc - DOI: 10.4025/actasciagron.v31i3.364 Agronomy
Faria, Flaviana Andrade; UNESP, Ilha Solteira; Bueno, César Júnior; APTA; Papa, Marli de Fátima Stradioto; UNESP, Ilha Solteira.
O fungo Sclerotium rolfsii causa grandes perdas em algumas culturas econômicas. Por produzir estruturas de resistência (escleródios), este fungo é de difícil controle. Há escassez de novos ingredientes ativos eficientes para o controle deste patógeno. Assim, o objetivo do presente trabalho foi verificar se existe atividade fungitóxica na planta Momordica charantia (melão-de-são-caetano), com potencial futuro para ser estudado no controle de S. rolfsii. Para isso, dois ensaios foram realizados, um in vitro (laboratório) e outro in vivo (câmara de crescimento). Em in vitro, escleródios do patógeno ficaram em contato com extratos hidroetanólico e aquoso de folhas e ramos de M. charantia e sem extrato por 7, 14, 21 e 28 dias. A sobrevivência dos escleródios...
Palavras-chave: 5.01.00.00-9 Agronomia extratos de plantas; Melão-de-são-caetano; Escleródios; Fungo fitopatogênico habitante do solo plants extract; Bitter gourd; Sclerotia; Soilborne phytopathogenic fungi.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/364
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Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani Electron. J. Biotechnol.
Shu,Canwei; Sun,Si; Chen,Jieling; Chen,Jianyi; Zhou,Erxun.
Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and contain a large quantity of polysaccharides, lipids, proteins and pigments. In order to extract high-quality total RNA from the sclerotia of R. solani, five methods, including E.Z.N.A.™ Fungal RNA Kit, sodium dodecyl sulfate (SDS)-sodium borate, SDS-polyvinylpyrrolidone (PVP), guanidinium thiocyanate (GTC) and modified Trizol, were compared in this study. Results The electrophoresis results showed that it failed to extract total RNA from the...
Tipo: Journal article Palavras-chave: Polyvinylpyrrolidone; Rhizoctonia solani; RT-PCR; Sclerotia; Sodium dodecyl sulfate; Total RNA extraction.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100009
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Compatibility and ectomycorrhiza formation among Pisolithus Isolates and Eucalyptus spp Rev. Bras. Ciênc. Solo
Pereira,Olinto Liparini; Costa,Maurício Dutra; Borges,Arnaldo Chaer; Araújo,Elza Fernandes; Kasuya,Maria Catarina Megumi.
Twenty-nine isolates of the ectomycorrhiza fungus Pisolithus sp. from different geographical and host origins were tested for their ability to form ectomycorrhizae on Eucalyptus grandis and E. urophylla seedlings under greenhouse conditions. The ectomycorrhiza-forming capacity of isolates varied greatly from one eucalypt species to the other. All isolates from Eucalyptus, nine from Pinus spp. and two isolates from unknown hosts formed mycorrhizae with E. grandis and E. urophylla. Root colonization rates varied from 0 to 5.2 % for all Pinus isolates and those from unknown hosts. Colonization rates for these isolates were lower than those observed for Eucalyptus isolates (0.8 to 89.4 %). Three isolates from unknown hosts formed mycorrhizae with neither...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ectomycorrhiza colonization; Eucalyptus grandis; Eucalyptus urophylla; Morphological characterization; Sclerotia.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832005000300003
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Cultivo de morchella esculenta (L.) Pers. y obtención de esclerocios in vitro Colegio de Postgraduados
Alvarado Castillo, Gerardo.
Morchella esculenta es un hongo comestible de alto valor comercial y la obtención de esclerocios es considerada como la clave para su cultivo. En este trabajo se evaluó la cepa IE-750 en ocho medios de cultivo sólido y seis en forma líquida, utilizando como parámetros el crecimiento micelial, la producción de biomasa, la habilidad para producir esclerocios y su biomasa. El mejor crecimiento micelial se obtuvo en el tratamiento con composta (T7: 53.87 cm2), y en cuanto a la biomasa, el medio de cultivo con levadura fue mejor (T3: 80.3 mg). Los tratamientos con gallinaza y composta, presentaron 40% y 80% de esclerocios, respectivamente, tanto en medio sólido como líquido. La mayor cantidad de biomasa de estas estructuras se presentó en el...
Tipo: Tesis Palavras-chave: Esclerocios; Composta; Medios de cultivo; Morchella esculenta; Maestría; Agroecosistemas Tropicales; Sclerotia; Compost; Culture media.
Ano: 2008 URL: http://hdl.handle.net/10521/1414
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Impact of Roundup Ready® Canola on Plant Pathogen and Its Suitability as Animal Feed: A case study in Canada Taiwan Agricultural Research Institute
H.C. Huang; R. Sharma; R.E. Blackshaw.
[[abstract]]The steady increase in world production of genetically modified (GM) or transgenic crops since their introduction in the 1990s has caused some concerns, regarding the bio-safety on persistence and stability of recombinant DNA from GM crops. A field experiment was established in 2000 at the Agriculture and Agri-Food Canada Lethbridge Research Centre to investigate the long-term environmental impacts of some GM crops approved for production in Canada, including GM canola, corn and potatoes. One of the objectives of this study was to investigate horizontal gene transfer (HGT) from Roundup Ready®(RR ) canola (Brassica napus event RT73) to Sclerotinia sclerotiorum , causal agent of stem blight of canola . The investigations focused on the cp4...
Palavras-chave: Bio-safety; Cp4 EPSPS gene; Sclerotia; Sclerotinia sclerotiorum; Canola; Brassica napus; Horizontal gene transfer; Roundup Ready® canola; Glyphosate Ecological and Environmental Biosafety of Transgenic Plants : proceedings of international symposium [[classification]]9.
Ano: 2006
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Liquid culture production of microsclerotia and submerged conidia by Trichoderma harzianum active against damping-off disease caused by Rhizoctonia solani. Repositório Alice
KOBORI, N. N.; MASCARIN, G. M.; JACKSON, M. A.; SCHISLER, D. A..
Media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L -1) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This is the first report of MS formation by T. harzianum or any species of Trichoderma. Furthermore, submerged conidia formation was induced by liquid culture media, but yields, desiccation tolerance, and storage stability varied with C:N ratio and carbon rate. Air-dried MS granules (<4 % moisture) retained excellent shelf life under cool and unrefrigerated storage...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Rhizoctonia solani; Trichoderma harzianum; Biopesticides; Soil-borne diseases; Sclerotia; Carbon nitrogen ratio.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1011851
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Production of caffeine alkaloid by Claviceps sorghi Trop. Plant Pathol.
Bogo,Amauri; Mantle,Peter Gregory; Boff,Mari Inês C.; Amarante,Cassandro V. T. do.
The ergot disease of sorghum (Sorghum bicolor), caused by the fungus Claviceps sorghi, restricted to the Indian sub-continent, is a disease in which the pathogen infects the florets, colonizing the unfertilized ovaries. Losses are higher in hybrid seed production fields due to a higher susceptibility of male sterile lines. The sclerotia of C. sorghi have never been found to contain alkaloids with a tetracyclic ergoline ring system, which is normal in most ergot pathogens. In this work, we show that sclerotia of C. sorghi contain caffeine alkaloid and the ability to produce it in vitro.
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro growth; Sclerotia; Alkaloid; Sorghum bicolor.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582003000400019
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Selection of Trichoderma spp. strains for the control of Sclerotinia sclerotiorum in soybean PAB
Haddad,Patrícia Elias; Leite,Luis Garrigós; Lucon,Cleusa Maria Mantovanello; Harakava,Ricardo.
Abstract: The objective of this work was to evaluate, in vitro and in vivo, the potential of Trichoderma spp. strains to control Sclerotinia sclerotiorum in soybeans (Glycine max) and to perform the molecular identification of the best perfoming strains. The effect of 120 strains of Trichoderma spp. on the viability of S. sclerotiorum sclerotia was evaluated in vitro through immersion in suspension of conidia from the antagonists and plating in culture medium. The best performing strains were evaluated in vivo, in a greenhouse, for control of the pathogen inoculated on 'Pintado' soybean seeds and plants. Of the 120 strains tested in vitro, 22 strains of Trichoderma spp. caused 100% inhibition of sclerotia germination. In the greenhouse, five strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Biological control; DNA sequencing; Sclerotia; White mold.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201140
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菌核鮑魚菇產生菌核之研究 Taiwan Agricultural Research Institute
陳美杏; 彭金騰; Meei-Hsing Chen;Jin-Torng Peng.
[[abstract]]利用稻草、山黃麻木屑或棉子殼等介質栽培菌核鮑魚菇 (Pleurotus tuber-regium),結果顯示以棉子殼栽培之菌核最大,而以山黃麻木屑栽培最小。就生物效率而言,以棉子殼栽培和稻草栽培兩者並未達顯著之差異,分別為31.73%及34.35%。以棉子殼為栽培基質,評估溫度對菌核鮑魚菇之菌核產生的影響,發現菌核的產量及生物效率隨著溫度的升高而有顯著降低的趨勢,其中菌核最適生產的溫度為20℃。進一步,將棉子殼之含水量調整於65%(w/w) 所產生之菌核產量最大。添加不同比例之米糠於棉子殼中,結果以不添加米糠者產量(150.04g/盆)及生物效率(42.87%)最高,其次是添加10%米糠者。
Palavras-chave: 菌核鮑魚菇 菌核 棉子殼 栽培介質 Pleurotus tuber-regium; Sclerotia; Cotton seed hull [[classification]]10.
Ano: 1999
Registros recuperados: 9
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