SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

SANTOS, S. N.; MELO, I. S. de.

Investigating bioactive components from microbial metabolites can provide potential source for drug discovery. There are a number of traditional antitumor compounds screening assays already described. In this study, we have developed an efficient method to search for antitumor compounds using the Oomycete Pythium aphanidermatum as model on agar plates for high throughtput screening. Antioomycete compounds showed cytotoxic effects in human cancer cells lines due to the possible similarity between the lipid cell membrane of the Pythium species and cancerous cells, suggesting the compounds act through similar mechanisms of inhibition. In this assay, i t is possible to evaluate hundreds of samples, with results in few hours. The Pythium assay was shown to be...

Tipo: Separatas

Palavras-chave: Antitumoral screening; Antifungal assay.; Pythium Aphanidermatum.; Antineoplastic agents; Screening; Secondary metabolites..

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1087004

A rapid screening method for cutinase producing microorganisms

BJM

Macedo,Gabriela Alves; Pio,Tatiana Fontes.

Both cutinase and lipase belong to the esterase group of enzymes (EC 3.1.1.X), which are capable of catalyzing the hydrolysis of ester bonds. Cutinase catalyzes the hydrolysis of cutin, an insoluble biopolyester which is the structural component of plant cuticles. As cutinase is an efficient catalyst in watery or organic media, it is potentially appropriate for the detergent, food and cosmetic industries. The objective of this work was to isolate microorganisms from plants and perform a pre-selection of molds showing esterase producing ability. The selected strains were then submitted to fermentation in media containing cutin. The lipolytic and cutinolytic activities of the supernatant were determined in order to differentiate lipase producers from...

A reliable begomovirus inoculation method for screening Lycopersicon esculentum lines

Palavras-chave: Cutinase; Esterase; Microbial enzymes; Screening.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400016

Horticultura Brasileira

Inoue-Nagata,Alice K; Nagata,Tatsuya; Ávila,Antonio C de; Giordano,Leonardo de B.

Due to failures of infection after mechanical inoculation of begomovirus on tomato (Lycopersicon esculentum Mill.) plants, an efficient and reliable begomovirus inoculation method was developed using the whitefly vector (Bemisia tabaci). Virus acquisition was carried out using a 'Tube-cage', made of a polypropylene tube, containing one to two day-old whiteflies and a tomato plant apex infected with a begomovirus isolate. After 48 h of acquisition access period, inoculation was done using a 'Ring-cage', containing viruliferous whiteflies, attached to the abaxial side of the leaf of young tomato plants. 48 hours after acquisition, the whiteflies were eliminated and the inoculated plants were incubated for 28 days under greenhouse conditions. Three...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Lycopersicon esculentum; Inoculation; Resistance; Screening; Geminivirus.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000300024

Accuracy and prognostic ability of the SARC-F questionnaire and Ishii's score in the screening of sarcopenia in geriatric inpatients

Li,Min; Kong,Yan; Chen,Hongcun; Chu,Aiqin; Song,Guiqi; Cui,Yan.

Sarcopenia remains poorly managed in clinical practice due to the lack of simple and accurate screening tools. This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow-up data were obtained by...

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation.

Palavras-chave: Sarcopenia; Muscle mass; Geriatric inpatients; Screening; Score.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900606

An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation

PAULO, E. M.; VASCONCELOS, M. P.; OLIVEIRA, I. S.; AFFE, H. M. J.; NASCIMENTO, R. dos S.; MELO, I. S. de; ROQUE, M. R. de A.; ASSIS, S. A. de.

Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs,...

Tipo: Separatas

Palavras-chave: Triagem; Exopolissacarídeos; Análise de laboratório; Polissacarídeo; Screening; Exopolysaccharides; Lactic acid bacteria.

Ano: 2012

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/963060

Ciênc. Tecnol. Aliment.

Paulo,Elinalva Maciel; Vasconcelos,Murilo Pinho; Oliveira,Ivelise Santiago; Affe,Helen Michelle de Jesus; Nascimento,Rosely; Melo,Itamar Soares de; Roque,Milton Ricardo de Abreu; Assis,Sandra Aparecida de.

The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was...

Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Palavras-chave: Screening; Exopolysaccharides; Lactic acid bacteria.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400011

BJID

Ferreira,Michele Berger; de-Paris,Fernanda; Paiva,Rodrigo Minuto; Nunes,Luciana de Souza.

ABSTRACT Group B Streptococcus is a causative agent of invasive neonatal infections. Maternal colonization by Streptococcus agalactiae is a necessary condition for vertical transmission, with efficient screening of pregnant women playing an essential role in the prevention of neonatal infections. In this study, we aimed to compare the performance of conventional polymerase chain reaction and real-time PCR assays as screening methods for S. agalactiae in pregnant women against the microbiological culture method considered as the gold-standard. A total of 130 samples from pregnant women were analyzed for sensitivity, specificity, positive predictive value, and negative predictive value. Statistical analysis was performed using the SPSS software, version...

Biodiversity of the oleaginous microorganisms in Tibetan Plateau

Palavras-chave: Streptococcus agalactiae; Pregnant women; Screening; Conventional PCR; Real-time PCR.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600449

BJM

Li,Shi Lin; Lin,Qiang; Li,Xin Ran; Xu,Hui; Yang,Yun Xi; Qiao,Dai Rong; Cao,Yi.

Microbial lipids, which are also known as single cell oils (SCO), are produced by oleaginous microorganisms including oleaginous bacteria, yeast, fungus and algae through converting carbohydrates into lipids under certain conditions. Due to its unique environment having extremely low temperature and anoxia, the Tibetan Plateau is amongst the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring, and lawn. By using a rapid, convenient screening method, we identified 31 strains of oleaginous microorganisms from different habitats in the Tibetan Plateau, which include wetlands, lawn, hot spring, alpine permafrost, and saline-alkali soil. Molecular identity analysis showed that they belong to 15 different species, 7...

Comparison of methods for urinary albumin determination in patients with type 1 diabetes

Palavras-chave: Oleaginous microorganisms; Screening; The Tibetan Plateau; Biodiversity.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200026

Khawali,C.; Andriolo,A.; Ferreira,S.R.G..

We tested the correlation of the albumin-to-creatinine ratio (A/C) in an early-morning urine sample, measured with a commercial kit (DCA 2000®), with the conventional immunoturbidimetric determination in the laboratory and with overnight albumin excretion rate (reference method). Fifty-five type 1 diabetic adolescents had their first-morning urine collected on the 1st and 8th day of the period. Urinary albumin and creatinine were determined immediately using the DCA 2000® kit. Samples were also stored for laboratory analysis. To evaluate the correlation between early-morning urinary A/C ratio and overnight albumin excretion rate, 16 subjects had a timed overnight urine collection. A/C ratios determined with the DCA 2000® kit and by the laboratory method...

Competing Screening Rules

Palavras-chave: Diabetic nephropathy; Microalbuminuria; Screening.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300008

AgEcon

Hendrikse, George W.J.; Hu, Yamei.

Various studies show that agricultural cooperatives behave differently than their investor-owned counterparts. One explanation may be that the internal decision making process differs in these two governance structures. A model is developed to explore how endogenous screening rules affect efficient organizational choices and industrial structures. It is shown that screening level choice may outweigh architecture choice and that screening rules are strategic substitutes. Conditions are derived under which cooperatives are efficient organizational forms. It is also shown that competition may increase the attractiveness of investor-owned firms and circumstances are determined in which cooperatives and investor owned firms coexist in equilibrium.

Tipo: Conference Paper or Presentation

Palavras-chave: Architecture; Screening; Cooperatives; Duopoly; Agribusiness; Q13.

Ano: 2005

URL: http://purl.umn.edu/24522

Demonstrating the need for chemical exposure characterisation in a microplate test system: Toxicity screening of sixteen pesticides on two marine microalgae

ArchiMer

Dupraz, Valentin; Stachowski-haberkorn, Sabine; Wicquart, Jérémy; Tapie, Nathalie; Budzinski, Hélène; Akcha, Farida.

Pesticides used in viticulture create a potential risk for the aquatic environment due to drift during application, runoff and soil leaching. The toxicity of sixteen pesticides and one metabolite were evaluated on the growth of two marine microalgae, Tisochrysis lutea and Skeletonema marinoi, in 96-h exposure assays conducted in microplates. For each substance, concentrations of stock solutions were analytically measured and abiotic assays were performed to evaluate the chemical stability of pesticides in microplates. For two chemicals, microalgae exposures were run simultaneously in microplates and culture flasks to compare EC50 calculated from the two exposure systems. Results from chemical analyses demonstrated the low stability of hydrophobic...

Tipo: Text

Palavras-chave: Microalgae; Microplates; Screening; Pesticides; Toxicity; Exposure characterisation.

Ano: 2019

URL: https://archimer.ifremer.fr/doc/00474/58558/61113.pdf

Downy mildew screening on S2 lines of Puerto Rico S1 lines of Thai composite white

Thai Agricultural

Virtucio, Robert H..

1 table

Palavras-chave: Corn; Maize; S1 lines; Puerto Rico; S2 lines; Thai composite white; Downy mildew; Screening; Disease resistance; ข้าวโพด; โรคราน้ำค้าง; ลูกเอส 1; เปอร์โตริโก; พันธุ์ผสมเปิดสีขาว.

Ano: 1971

URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3785

Estimativa do número de amostras de solo para caracterizar elementos químicos utilizando fluorescência de raios X portátil em áreas contaminadas: estudo de caso em Resende.

Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system

TROYACK, I. B.; VÖN MUHLEN, C.; VASQUES, G. de M.; TEIXEIRA, W. G..

A representatividade da amostragem é fundamental para embasar a tomada de decisão, uma vez que a heterogeneidade da matriz é o principal fator para incerteza dos dados nos estudos de gerenciamento de áreas contaminadas. Neste estudo, coletaram-se 112 amostras de solo superficial em malha quadrada de 1 x 1 m, em uma área 15 x 6 m, no campus regional de Resende da Universidade do Estado do Rio de Janeiro. Anteriormente, no terreno funcionaram empresas de produção de filmes radiográficos e purificação de prata metálica. As amostras de solo foram analisadas por pXRF, método de screening para metais. Com base nos dados levantados, calculou-se, por meio de métodos estatísticos, a quantidade de amostras que permitiriam uma caracterização representativa da área,...

Tipo: Artigo de periódico

Palavras-chave: Investigação ambiental; Fluorescência de raios X portátil; Contaminação por metais; Solo; Amostragem; Screening.

Ano: 2021

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135482

Chuang,K.A.; Lieu,C.H.; Tsai,W.J.; Wu,M.H.; Chen,Y.C.; Liao,J.F.; Wang,C.C.; Kuo,Y.C..

Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for the screening of anti-Wnt/β-catenin agents, we designed a bi-functional pGL4-TOP reporter plasmid that contained 3X β-catenin/LEF/TCF binding sites and a selectable marker. After transfection and hygromycin B selection, HEK 293-TOP and Jurkat-TOP stable clones were established. The luciferase activity in the stable clone was enhanced by the recombinant Wnt-3A (rWnt-3A; 100-400 ng/mL) and GSK3β inhibitor...

Evaluation of Resistance of Cucumber Cultivars to the Vegetable Leafminer (Liriomyza sativae Blanchard) (Diptera: Agromyzidae) in Greenhouse

Palavras-chave: Cancer; Plasmids; Reporter assay; Screening; Wnt; Β-catenin.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000003

Chilean J. Agric. Res.

Basij,Moslem; Askarianzaeh,Alireza; Asgari,Shahriyar; Moharramipou,Saeid; Rafezi,Ramin.

The leafminer, Liriomyza sativae Blanchard, is distributed around the world and is an important pest of vegetables and ornamentals. Given the resistance potential of the leafminer to current insecticides, the use of resistant plant cultivars and parasitoids could be effective integrated pest management (IPM) strategies against it. Cucumber (Cucumis sativus L.) is a preferred host for this insect. Seventeen cultivars of cucumber have been evaluated to study resistance mechanisms to L. sativae. All cucumber cultivars were evaluated in screening tests in greenhouse with indices such as the number of leafminer stings, the number of larval mines, the proportion of larval mines to leafminer stings, and the rate of injury. Significant differences (p < 0.01)...

Tipo: Journal article

Palavras-chave: Cucumber cultivar; Screening; Antibiosis; Screening.

Ano: 2011

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300008

Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase.

Interference of propylene glycol with the hole-board test

SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F..

Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase,...

Tipo: Artigo de periódico

Palavras-chave: Enzima; Clonagem; Programa de Computador; Peptídeo; Metagenomics; Enzymes; Cloning (animals); Signal peptide; Prevotella; Computer software; Screening.

Ano: 2021

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129624

Lourenço da Silva,A.; Elisabetsky,E..

Experimental drugs and/or plant extracts are often dissolved in solvents, including propylene glycol. Nevertheless, there is evidence for psychoactive properties of this alcohol. In this study we found that in the hole-board test 10% propylene glycol did not modify the head-dipping behavior. However, 30% propylene glycol induced an increase in the number of head-dips (46.92 ± 2.37 compared to 33.83 ± 4.39, P<0.05, ANOVA/Student-Newman-Keuls), an effect comparable to that obtained with 0.5 mg/kg diazepam (from 33.83 ± 4.39 to 54 ± 3.8, P<0.01, ANOVA/Student-Newman-Keuls). These results demonstrate that 30% propylene glycol has significant anxiolytic effects in this model and therefore cannot be used as an innocuous solvent.

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Screening; Anxiolytic drugs; Propylene glycol; Diazepam; Solvents; Hole-board.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400016

Iron Uptake by Fungi Isolated from Arcelor Mittal -Annaba- in the Northeast of Algeria

Rev. Bras. Ciênc. Avic.

Bourzama,G; Ouled-Haddar,H; Marrouche,M; Aliouat,A.

ABSTRACT The Metal pollution is one of the major risks in the world nowadays. Iron is an essential metal for growth and proliferation of a vast majority of organisms, but it can be toxic to human health and other living beings in the environment at high concentrations due to its increased industrial activity. Fungi have a remarkable capacity to uptake and detoxify iron metal using different mechanisms such as bioaccumulation. Thus, the aim of this work is to study the ability of iron uptake by the fungal strains isolated from Arcelor Mittal -Annaba- in Algeria. Three strains were screened at high concentration of iron (1 g/l) and their capacity to uptake iron has been studied on Czapek Yeast Agar medium. The amounts of uptaken iron ions were estimated in...