Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 54
Primeira ... 123 ... Última
Imagem não selecionada

Imprime registro no formato completo
A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D.
Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this...
Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf
Imagem não selecionada

Imprime registro no formato completo
A rapid screening method for cutinase producing microorganisms BJM
Macedo,Gabriela Alves; Pio,Tatiana Fontes.
Both cutinase and lipase belong to the esterase group of enzymes (EC 3.1.1.X), which are capable of catalyzing the hydrolysis of ester bonds. Cutinase catalyzes the hydrolysis of cutin, an insoluble biopolyester which is the structural component of plant cuticles. As cutinase is an efficient catalyst in watery or organic media, it is potentially appropriate for the detergent, food and cosmetic industries. The objective of this work was to isolate microorganisms from plants and perform a pre-selection of molds showing esterase producing ability. The selected strains were then submitted to fermentation in media containing cutin. The lipolytic and cutinolytic activities of the supernatant were determined in order to differentiate lipase producers from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutinase; Esterase; Microbial enzymes; Screening.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400016
Imagem não selecionada

Imprime registro no formato completo
A reliable begomovirus inoculation method for screening Lycopersicon esculentum lines Horticultura Brasileira
Inoue-Nagata,Alice K; Nagata,Tatsuya; Ávila,Antonio C de; Giordano,Leonardo de B.
Due to failures of infection after mechanical inoculation of begomovirus on tomato (Lycopersicon esculentum Mill.) plants, an efficient and reliable begomovirus inoculation method was developed using the whitefly vector (Bemisia tabaci). Virus acquisition was carried out using a 'Tube-cage', made of a polypropylene tube, containing one to two day-old whiteflies and a tomato plant apex infected with a begomovirus isolate. After 48 h of acquisition access period, inoculation was done using a 'Ring-cage', containing viruliferous whiteflies, attached to the abaxial side of the leaf of young tomato plants. 48 hours after acquisition, the whiteflies were eliminated and the inoculated plants were incubated for 28 days under greenhouse conditions. Three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Lycopersicon esculentum; Inoculation; Resistance; Screening; Geminivirus.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000300024
Imagem não selecionada

Imprime registro no formato completo
A simple screening technique for salinity tolerance in rice: germination rate under stress International Rice Research Institute
Abeysiriwardena, D. S. de Z..
p.78-79
Palavras-chave: Salinity; Salt tolerance; Germination; Screening; Cultivars.
Ano: 2004 URL: http://hdl.handle.net/123456789/1068
Imagem não selecionada

Imprime registro no formato completo
Accuracy and prognostic ability of the SARC-F questionnaire and Ishii's score in the screening of sarcopenia in geriatric inpatients BJMBR
Li,Min; Kong,Yan; Chen,Hongcun; Chu,Aiqin; Song,Guiqi; Cui,Yan.
Sarcopenia remains poorly managed in clinical practice due to the lack of simple and accurate screening tools. This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow-up data were obtained by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sarcopenia; Muscle mass; Geriatric inpatients; Screening; Score.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900606
Imagem não selecionada

Imprime registro no formato completo
An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation. Repositório Alice
PAULO, E. M.; VASCONCELOS, M. P.; OLIVEIRA, I. S.; AFFE, H. M. J.; NASCIMENTO, R. dos S.; MELO, I. S. de; ROQUE, M. R. de A.; ASSIS, S. A. de.
Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triagem; Exopolissacarídeos; Análise de laboratório; Polissacarídeo; Screening; Exopolysaccharides; Lactic acid bacteria.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/963060
Imagem não selecionada

Imprime registro no formato completo
An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation Ciênc. Tecnol. Aliment.
Paulo,Elinalva Maciel; Vasconcelos,Murilo Pinho; Oliveira,Ivelise Santiago; Affe,Helen Michelle de Jesus; Nascimento,Rosely; Melo,Itamar Soares de; Roque,Milton Ricardo de Abreu; Assis,Sandra Aparecida de.
The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Screening; Exopolysaccharides; Lactic acid bacteria.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400011
Imagem não selecionada

Imprime registro no formato completo
Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women BJID
Ferreira,Michele Berger; de-Paris,Fernanda; Paiva,Rodrigo Minuto; Nunes,Luciana de Souza.
ABSTRACT Group B Streptococcus is a causative agent of invasive neonatal infections. Maternal colonization by Streptococcus agalactiae is a necessary condition for vertical transmission, with efficient screening of pregnant women playing an essential role in the prevention of neonatal infections. In this study, we aimed to compare the performance of conventional polymerase chain reaction and real-time PCR assays as screening methods for S. agalactiae in pregnant women against the microbiological culture method considered as the gold-standard. A total of 130 samples from pregnant women were analyzed for sensitivity, specificity, positive predictive value, and negative predictive value. Statistical analysis was performed using the SPSS software, version...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus agalactiae; Pregnant women; Screening; Conventional PCR; Real-time PCR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600449
Imagem não selecionada

Imprime registro no formato completo
Avaliação da precocidade de emissão da raiz primária em milho e parâmetros de parte aérea para secreening de Al, e, solução nutritiva. Repositório Alice
DURAES, F. O. M.; OLIVEIRA, A. C. de; NEIS, L.; VERGINASSI, A.; BRITTO, D.D. de S..
2001
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Aluminio; Tolerancia; Screening; Zea mys; Maize; Aluminium; Tolerance.
Ano: 2001 URL: http://www.alice.cnptia.embrapa.br/handle/doc/485132
Imagem não selecionada

Imprime registro no formato completo
Avaliação e seleção de acessos tropicais de milho em solos de baixa fertilidade com limitações em fósforo. Repositório Alice
SANTOS, M.X.; CARVALHO, H.W.L.; LEITE, C.E.P.; ANDRADE, R.V.; VASCONCELLOS, C.A..
1998
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Melhoramento; Tropical; Fosforo; Deficiencia mineral; Zea mays; Maize; Screening; Mineral deficiency; Phosphorus; Breeding.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/480583
Imagem não selecionada

Imprime registro no formato completo
Biodiversity of the oleaginous microorganisms in Tibetan Plateau BJM
Li,Shi Lin; Lin,Qiang; Li,Xin Ran; Xu,Hui; Yang,Yun Xi; Qiao,Dai Rong; Cao,Yi.
Microbial lipids, which are also known as single cell oils (SCO), are produced by oleaginous microorganisms including oleaginous bacteria, yeast, fungus and algae through converting carbohydrates into lipids under certain conditions. Due to its unique environment having extremely low temperature and anoxia, the Tibetan Plateau is amongst the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring, and lawn. By using a rapid, convenient screening method, we identified 31 strains of oleaginous microorganisms from different habitats in the Tibetan Plateau, which include wetlands, lawn, hot spring, alpine permafrost, and saline-alkali soil. Molecular identity analysis showed that they belong to 15 different species, 7...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oleaginous microorganisms; Screening; The Tibetan Plateau; Biodiversity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200026
Imagem não selecionada

Imprime registro no formato completo
CHARACTERIZATION OF THE OXIDATIVE ENZYME POTENTIAL IN WILD WHITE ROT FUNGI FROM THE SUBTROPICAL FOREST OF MISIONES (ARGENTINA) Acta biol.Colomb.
FONSECA,María Isabel; ZAPATA,Pedro Darío; VILLALBA,Laura Lidia; FARIÑA,Julia Inés.
This research aimed to evaluate the potential of several native white rot fungi (WRF) isolated from subtropical environments of Misiones (Argentina) to produce different ligninolytic enzymes. Coriolus versicolor f. antarcticus BAFC 266, Pycnoporus sanguineus BAFC 2126 and Phlebia brevispora BAFC 633 showed the highest phenoloxidase activity. Ganoderma applanatum strain E, P. sanguineus BAFC 2126 and P. brevispora BAFC 633 revealed marked laccase and peroxidase activity. C. versicolor f. antarcticus, G. applanatum (strain A) and Trametes villosa, gave high positive reactions with 2,6-dimethoxyphenol oxidation at the lowest tested pH. C. versicolor f. antarcticus, G. applanatum strains D and F, T. elegans BAFC 2127and T. villosa, showed the highest manganese...
Tipo: Journal article Palavras-chave: Oxidative enzymes; Screening; White-rot fungi.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100006
Imagem não selecionada

Imprime registro no formato completo
Comparison of methods for urinary albumin determination in patients with type 1 diabetes BJMBR
Khawali,C.; Andriolo,A.; Ferreira,S.R.G..
We tested the correlation of the albumin-to-creatinine ratio (A/C) in an early-morning urine sample, measured with a commercial kit (DCA 2000®), with the conventional immunoturbidimetric determination in the laboratory and with overnight albumin excretion rate (reference method). Fifty-five type 1 diabetic adolescents had their first-morning urine collected on the 1st and 8th day of the period. Urinary albumin and creatinine were determined immediately using the DCA 2000® kit. Samples were also stored for laboratory analysis. To evaluate the correlation between early-morning urinary A/C ratio and overnight albumin excretion rate, 16 subjects had a timed overnight urine collection. A/C ratios determined with the DCA 2000® kit and by the laboratory method...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetic nephropathy; Microalbuminuria; Screening.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300008
Imagem não selecionada

Imprime registro no formato completo
Competing Screening Rules AgEcon
Hendrikse, George W.J.; Hu, Yamei.
Various studies show that agricultural cooperatives behave differently than their investor-owned counterparts. One explanation may be that the internal decision making process differs in these two governance structures. A model is developed to explore how endogenous screening rules affect efficient organizational choices and industrial structures. It is shown that screening level choice may outweigh architecture choice and that screening rules are strategic substitutes. Conditions are derived under which cooperatives are efficient organizational forms. It is also shown that competition may increase the attractiveness of investor-owned firms and circumstances are determined in which cooperatives and investor owned firms coexist in equilibrium.
Tipo: Conference Paper or Presentation Palavras-chave: Architecture; Screening; Cooperatives; Duopoly; Agribusiness; Q13.
Ano: 2005 URL: http://purl.umn.edu/24522
Imagem não selecionada

Imprime registro no formato completo
Corn and sorghum insects 1983 annual report Thai Agricultural
Meksongsee, B.; Kongkanjana, M.; Chawanapong, C.; Chunhawang, W.; Jumrernma, K.; Wongkobrat, A..
6 tables
Palavras-chave: Corn; Maize; Sorghum; Insect pests; Stem borers; Ostrinia furnacalis; Shoot fly; Atherigona soccata; Chemical control; Variety trials; Yields; Screening; ข้าวโพด; ข้าวฟ่าง; แมลงศัตรูพืช; การควบคุมด้วยสารเคมี; ไตรโคเดอร์มา; หนอนเจาะลำต้น; หนอนแมลงวันเจาะยอดข้าวฟ่าง.
Ano: 1983 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4144
Imagem não selecionada

Imprime registro no formato completo
Demonstrating the need for chemical exposure characterisation in a microplate test system: Toxicity screening of sixteen pesticides on two marine microalgae ArchiMer
Dupraz, Valentin; Stachowski-haberkorn, Sabine; Wicquart, Jérémy; Tapie, Nathalie; Budzinski, Hélène; Akcha, Farida.
Pesticides used in viticulture create a potential risk for the aquatic environment due to drift during application, runoff and soil leaching. The toxicity of sixteen pesticides and one metabolite were evaluated on the growth of two marine microalgae, Tisochrysis lutea and Skeletonema marinoi, in 96-h exposure assays conducted in microplates. For each substance, concentrations of stock solutions were analytically measured and abiotic assays were performed to evaluate the chemical stability of pesticides in microplates. For two chemicals, microalgae exposures were run simultaneously in microplates and culture flasks to compare EC50 calculated from the two exposure systems. Results from chemical analyses demonstrated the low stability of hydrophobic...
Tipo: Text Palavras-chave: Microalgae; Microplates; Screening; Pesticides; Toxicity; Exposure characterisation.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00474/58558/61113.pdf
Imagem não selecionada

Imprime registro no formato completo
Detecção de condições de estresse em plantas e potencial para "screening" em milho através da fluorescência da clorofila. Repositório Alice
DURAES, F. O. M.; OLIVEIRA, A. C.; MAGALHAES, P. C.; MARTINEZ, C.A..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Milho; Estresse; Ecofisiologia; Screening; Zea mays; Maize; Stress; Ecophysiology.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484141
Imagem não selecionada

Imprime registro no formato completo
Development of preharvest sprouting tolerance screening technique in rice International Rice Research Institute
Mahbub, A. A.; Rahman, M. S.; Khanam, M.; Gomosta, A. R..
p.50-51
Palavras-chave: Preharvest sprouting; Tolerance; Screening; Varieties; Techniques; Bangladesh.
Ano: 2005 URL: http://hdl.handle.net/123456789/1140
Imagem não selecionada

Imprime registro no formato completo
Downy mildew screening on S2 lines of Puerto Rico S1 lines of Thai composite white Thai Agricultural
Virtucio, Robert H..
1 table
Palavras-chave: Corn; Maize; S1 lines; Puerto Rico; S2 lines; Thai composite white; Downy mildew; Screening; Disease resistance; ข้าวโพด; โรคราน้ำค้าง; ลูกเอส 1; เปอร์โตริโก; พันธุ์ผสมเปิดสีขาว.
Ano: 1971 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3785
Imagem não selecionada

Imprime registro no formato completo
Evaluation of anti-Wnt/β-catenin signaling agents by pGL4-TOP transfected stable cells with a luciferase reporter system BJMBR
Chuang,K.A.; Lieu,C.H.; Tsai,W.J.; Wu,M.H.; Chen,Y.C.; Liao,J.F.; Wang,C.C.; Kuo,Y.C..
Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for the screening of anti-Wnt/β-catenin agents, we designed a bi-functional pGL4-TOP reporter plasmid that contained 3X β-catenin/LEF/TCF binding sites and a selectable marker. After transfection and hygromycin B selection, HEK 293-TOP and Jurkat-TOP stable clones were established. The luciferase activity in the stable clone was enhanced by the recombinant Wnt-3A (rWnt-3A; 100-400 ng/mL) and GSK3β inhibitor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer; Plasmids; Reporter assay; Screening; Wnt; Β-catenin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000003
Registros recuperados: 54
Primeira ... 123 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional