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1-MCP efficiency in quality of ornamental peppers Horticultura Brasileira
Ribeiro,Wellington S; Carneiro,Clebson dos S; França,Christiane de Fátima M; Pinto,Cleide Maria F; Lima,Paula Cristina C; Finger,Fernando L.
ABSTRACT 1-MCP has been effectively used as an antagonist of ethylene deleterious actions in flowers and ornamental plants. The objective was to evaluate the efficiency of 1-MCP (0.0, 0.5, 1.0 and 1.5 g m-3) in maintaining post-production quality of exogenous ethylene sensitive ornamental pepper. Two commercial varieties were evaluated: Pimenta Colorida and Pimentão Ornamental, both Capsicum annuum. The pre-treatment with 1-MCP, regardless of the applied concentration, was effective in blocking the ethylene action reducing the leaves abscission, flowers and fruits of both genotypes by 100%. Plants pretreated with 1-MCP had postproduction life increased by eight and nine days for Pimenta Colorida and Pimentão Ornamental, respectively. The 1-MCP application...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capsicum annuum; Ornamental plants; Sensitivity; Transport.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400510
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A mezőgazdasági területek érzékenységének és adaptációs képességének mérési lehetőségei AgEcon
Farkas-Fekete, Maria.
A tanulmány alapvető célja az, hogy hozzájáruljon az érzékenység, sérülékenység és adaptációs képesség vizsgálatának módszertanához. A kutatások eredményei felhívják a figyelmet arra, hogy bár az európai vidék összessége egyre sérülékenyebbé válik a különböző globális kihívásokra, a veszélyeztetettség mértéke régiónként eltérő. A kutatások eredményei megerősítették az IPCC 3. jelentésében megfogalmazott hipotéziseket, melyek szerint a gazdag országok ugyanolyan veszélyeztetettség mellett is kevésbé sérülékenyek, mivel nagyobb az alkalmazkodóképességük. A klímaváltozás, amely pozitív és negatív hatást is gyakorol Európa mezőgazdaságára, módosítja az eddigi komparatív előnyöket, illetve hátrányokat. Gazdasági szempontból Dél- és Közép-Kelet-Európa minden...
Tipo: Journal Article Palavras-chave: Fenntarthatóság; Klímaváltozás; Érzékenység; Sérülékenység; Veszélyeztetettség; Adaptáció; Sustainability; Climate change; Vulnerability; Sensitivity; Adaptability; Agricultural and Food Policy; Environmental Economics and Policy.
Ano: 2009 URL: http://purl.umn.edu/92481
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Accommodating covariates in receiver operating characteristic analysis AgEcon
Janes, Holly; Longton, Gary M.; Pepe, Margaret S..
Classification accuracy is the ability of a marker or diagnostic test to discriminate between two groups of individuals, cases and controls, and is commonly summarized by using the receiver operating characteristic (ROC) curve. In studies of classification accuracy, there are often covariates that should be incorporated into the ROC analysis. We describe three ways of using covariate information. For factors that affect marker observations among controls, we present a method for covariate adjustment. For factors that affect discrimination (i.e., the ROC curve), we describe methods for modeling the ROC curve as a function of covariates. Finally, for factors that contribute to discrimination, we propose combining the marker and covariate information, and we...
Tipo: Article Palavras-chave: Roccurve; Comproc; Rocreg; Receiver operating characteristic analysis; ROC; Covariates; Sensitivity; Specificity; Research Methods/ Statistical Methods.
Ano: 2009 URL: http://purl.umn.edu/122695
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Antifungal activity of lectins against yeast of vaginal secretion BJM
Gomes,Bruno Severo; Siqueira,Ana Beatriz Sotero; Maia,Rita de Cássia Carvalho; Giampaoli,Viviana; Teixeira,Edson Holanda; Arruda,Francisco Vassiliepe Sousa; Nascimento,Kyria Santiago do; Lima,Adriana Nunes de; Souza-Motta,Cristina Maria; Cavada,Benildo Sousa; Porto,Ana Lúcia Figueiredo.
Lectins are carbohydrate-binding proteins of non-imune origin. This group of proteins is distributed widely in nature and they have been found in viruses, microorganisms, plants and animals. Lectins of plants have been isolated and characterized according to their chemical, physical-chemical, structural and biological properties. Among their biological activities, we can stress its fungicidal action. It has been previously described the effect of the lectins Dviol, DRL, ConBr and LSL obtained from the seeds of leguminous plants on the growth of yeasts isolated from vaginal secretions. In the present work the experiments were carried out in microtiter plates and the results interpreted by both methods: visual observations and a microplate reader at 530nm....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Sensitivity; Lectins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200042
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Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite BJM
Iñiguez-Moreno,Maricarmen; Gutiérrez-Lomelí,Melesio; Guerrero-Medina,Pedro Javier; Avila-Novoa,María Guadalupe.
Abstract The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37 °C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p > 0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61 ± 0.13 Log10 CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91 ± 0.44 Log10 CFU/cm2 (p < 0.05). The mono and dual-species biofilms were subjected to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilms; Salmonella spp.; Staphylococcus aureus; Disinfectants; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200310
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Chemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México BJM
Aguilar-Barragan,Alejandra; García-Torres,Ana Elisa; Odriozola-Casas,Olga; Macedo-Raygoza,Gloria; Ogura,Tetsuya; Manzo-Sánchez,Gilberto; James,Andrew C.; Islas-Flores,Ignacio; Beltrán-García,Miguel J..
The chemical management of the black leaf streak disease in banana caused by Mycosphaerella fijiensis (Morelet) requires numerous applications of fungicides per year. However this has led to fungicide resistance in the field. The present study evaluated the activities of six fungicides against the mycelial growth by determination of EC50 values of strains collected from fields with different fungicide management programs: Rustic management (RM) without applications and Intensive management (IM) more than 25 fungicide application/year. Results showed a decreased sensitivity to all fungicides in isolates collected from IM. Means of EC50 values in mg L-1 for RM and IM were: 13.25 ± 18.24 and 51.58 ± 46.14 for azoxystrobin, 81.40 ± 56.50 and 1.8575 ± 2.11 for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banana; Fungicides; Mycosphaerella fijiensis; Sensitivity; Sigatoka.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100051
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Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD BJM
Leal,C.A.G.; Carvalho-Castro,G.A.; Cottorello,A.C.; Leite,R.C.; Figueiredo,H.C.P..
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clinical samples; Sensitivity; Real-time PCR; WSSV; WSD.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
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Comparative assessment of three common algorithms for estimating the variance of the area under the nonparametric receiver operating characteristic curve AgEcon
Cleves, Mario A..
The area under the receiver operating characteristic (ROC) curve is often used to summarize and compare the discriminatory accuracy of a diagnostic test or modality, and to evaluate the predictive power of statistical models for binary outcomes. Parametric maximum likelihood methods for fitting of the ROC curve provide direct estimates of the area under the ROC curve and its variance. Nonparametric methods, on the other hand, provide estimates of the area under the ROC curve, but do not directly estimate its variance. Three algorithms for computing the variance for the area under the nonparametric ROC curve are commonly used, although ambiguity exists about their behavior under diverse study conditions. Using simulated data, we found similar asymptotic...
Tipo: Journal Article Palavras-chave: Receiver operating characteristic (ROC) curve; Trapezoidal rule; Sensitivity; Specificity; Discriminatory accuracy; Predictive power; Research Methods/ Statistical Methods.
Ano: 2002 URL: http://purl.umn.edu/116008
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Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan BJM
Khan,Anwar Sheed; Ali,Sajid; Khan,Muhammad Tahir; Ahmed,Sajjad; Khattak,Yasir; Abduljabbar,; Irfan,Muhammad; Sajjad,Wasim.
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n = 390) to 47% (n = 347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GeneXpert; LED-FM microscopy; Sensitivity; Specificity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400909
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Convenient preparation of picolinyl derivatives from fatty acid esters ArchiMer
Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal.
It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation....
Tipo: Text Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf
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Desiccation tolerance of Tapirira obtusa seeds collected from different environments Rev. bras. sementes
Pereira,Wilson Vicente Souza; Faria,José Márcio Rocha; Tonetti,Olivia Alvina Oliveira; Silva,Edvaldo Aparecido Amaral da.
This study was aimed at evaluating the desiccation sensitivity in seeds of the tree Tapirira obtusa (Benth.) J. D. Mitchell collected from three different environments and subjected to two distinct drying speeds. Seeds were collected from a rocky area, in the "Cerrado", and in a riparian forest area, in the region of municipality of Lavras, State of Minas Gerais. The seeds were subjected to drying with magnesium chloride (slow drying) or silica gel (fast drying), into closed environment, until moisture contents of 40%, 30%, 20% and 10%, considering as control, the percentage of germination at the initial moisture content in each environment, which varied from 47% to 50%. Percentages of germination and normal seedlings as well as germination speed index...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Drying; Germination; Sensitivity; Dehydration.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000300005
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Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit ArchiMer
Garcia-martinez, Gonzalo; Alonso Bustabad, Enrique; Perrot, Hubert; Gabrielli, Claude; Bucur, Bogdan; Lazerges, Mathieu; Rose, Daniel; Rodriguez-pardo, Loreto; Farina, Jose; Compere, Chantal; Arnau Vives, Antonio.
This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.
Tipo: Text Palavras-chave: DNA sequence detection; Quartz crystal microbalance; Electronic oscillator; Sensitivity; Frequency noise; Resolution.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00044/15487/12886.pdf
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Differences in performance among four indices used to evaluate diversity in planktonic ecosystems ArchiMer
Beaugrand, G; Edwards, M.
The performance of four common estimators of diversity are investigated using calanoid copepod data from the Continuous Plankton Recorder (CPR) survey. The region of the North Atlantic and the North Sea was divided into squares of 400 nautical miles for each 2-month period. For each 144 possible cases, Pielou's pooled quadrat method was performed with the aims of determining asymptotic diversity and investigating the CPR sample-size dependence of diversity estimators. It is shown that the performance of diversity indices may greatly vary in space and time (at a seasonal scale). This dependence is more pronounced in higher diverse environments and when the sample size is small. Despite results showing that all estimators underestimate the 'actual'...
Tipo: Text Palavras-chave: Programme continuous plankton recorder; Copépodes; Indices de diversité; Taille de l’échantillonnage; Sensibilité; Continuous Plankton Recorder survey; Copepods; Diversity indices; Sample size; Sensitivity.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00322/43343/42884.pdf
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DIFFERENTIAL TOLERANCE OF PUMPKIN SPECIES TO BENTAZON, METRIBUZIN, TRIFLURALIN, AND OXYFLUORFEN Planta Daninha
NOSRATTI,I.; MAHDAVI-RAD,S.; HEIDARI,H.; SAEIDI,M..
ABSTRACT Response of pumpkin species including Cucurbita pepo convar. Pepo, Cucurbita moschata Duch, Cucurbita pepo, Cucurbita maxima, and Lagenaria vulgaris to bentazon, trifluralin, metribuzin, and oxyfluorfen was evaluated in Outdoor pot experiments in 2014 and 2015. Different postemergence doses (bentazon and oxyfluorfen) and preplant incorporated (metribuzin and trifluralin) herbicides were evaluted on pumpkin species at various growth stages. Results showed that the sensitivity of pumpkins species to applied herbicide varied greatly among tested species. On overall, dry weights of Cucurbita spp. were reduced by 12.50%, 48.60%, 23%, and 73.13% when pumpkin was treated with trifluralin, metribuzin, bentazon and oxyfluorfen, respectively. Pumpkin crops...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broadleaf weeds; Crop; Dominant weeds; Sensitivity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100264
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Estimation of sensitivity and specificity arising from validity studies with incomplete designs AgEcon
Reichenheim, Michael E.; Ponce de Leon, Antonio.
This insert introduces valides, validesi, and validesu, a set of commands that allow the calculations of point and precision estimates of sensitivity and specificity obtained in validity studies with incomplete designs.
Tipo: Journal Article Palavras-chave: Validity; Sensitivity; Specificity; Incomplete design; Research Methods/ Statistical Methods.
Ano: 2002 URL: http://purl.umn.edu/116004
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Evaluation of a 1-h 75-g oral glucose tolerance test in the diagnosis of gestational diabetes BJMBR
Campos,M.A.A.; Reichelt,A.A.J.; Façanha,C.; Forti,A.C.; Schmidt,M.I..
In order to evaluate the performance of a 1-h 75-g oral glucose tolerance test (OGTT) for the diagnosis of gestational diabetes mellitus (GDM), a cohort of 4998 women, 20 years or older, without previous diabetes being treated in prenatal care clinics in Brazil answered a questionnaire and performed a 75-g OGTT including fasting, 1-h and 2-h glucose measurements between their 24th and 28th gestational weeks. Pregnancy outcomes were transcribed from medical registries. GDM was defined according to WHO criteria (fasting: ≥126 mg/dL; 2-h value: ≥140 mg/dL) and macrosomia as a birth weight equal to or higher than 4000 g. Areas under the receiver operator characteristic curve (AUC) were compared and diagnostic properties of various cut-off points were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gestational diabetes; Diagnosis; Macrosomia; 1-h OGTT; Sensitivity; Specificity.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000800008
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Evaluation of the performance of different plastics used to seal nylon cDNA arrays Ciência e Agrotecnologia
Costa Netto,Antônio Paulino da; Drummond,Rodrigo Duarte; Felix,Juliana de Maria; Jorge,Renato Atílio; Menossi,Marcelo.
cDNA arrays are a powerful tool for discovering gene expression patterns. Nylon arrays have the advantage that they can be re-used several times. A key issue in high throughput gene expression analysis is sensitivity. In the case of nylon arrays, signal detection can be affected by the plastic bags used to keep membranes humid. In this study, we evaluated the effect of five types of plastics on the radioactive transmittance, number of genes with a signal above the background, and data variability. A polyethylene plastic bag 69 μm thick had a strong shielding effect that blocked 68.7% of the radioactive signal. The shielding effect on transmittance decreased the number of detected genes and increased the data variability. Other plastics which were thinner...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Signal detection; Nylon arrays; Sensitivity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000700031
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Initial identification and sensitivity to antimicrobial agents of Salmonella sp.isolated from poultry products in the state of Ceara, Brazil Rev. Bras. Ciênc. Avic.
Oliveira,WF; Cardoso,WM; Salles,RPR; Romão,JM; Teixeira,RSC; Câmara,SR; Siqueira,AA; Marques,LCL.
The objective of this research was to isolate and to verify the sensitivity to antimicrobial agents of strains of Salmonella sp. isolated from poultry products in the state of Ceara, Brazil. A total number of 114 samples was collected from 63 broiler carcasses derived from two processing plants and two supermarkets, and 51 excreta samples were collected in broiler farms located in the state of Ceara, which used three live production stages. Each excreta sample consisted of a fresh excreta pool from 100 birds. Samples were submitted to microbiological analyses, and the isolated Salmonella strains were tested for antimicrobial sensitivity. No Salmonella was isolated from excreta samples, while broiler carcass samples showed a high contamination rate of11.8%....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella sp; Identification; Sensitivity; Poultry products.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300010
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Insights into the Short-Term Tidal Variability of Multibeam Backscatter from Field Experiments on Different Seafloor Types ArchiMer
Montereale-gavazzi, Giacomo; Roche, Marc; Degrendele, Koen; Lurton, Xavier; Terseleer, Nathan; Baeye, Matthias; Francken, Frederic; Van Lancker, Vera.
Three experiments were conducted in the Belgian part of the North Sea to investigate short-term variation in seafloor backscatter strength (BS) obtained with multibeam echosounders (MBES). Measurements were acquired on predominantly gravelly (offshore) and sandy and muddy (nearshore) areas. Kongsberg EM3002 and EM2040 dual MBES were used to carry out repeated 300-kHz backscatter measurements over tidal cycles (similar to 13 h). Measurements were analysed in complement to an array of ground-truth variables on sediment and current nature and dynamics. Seafloor and water-column sampling was used, as well as benthic landers equipped with different oceanographic sensors. Both angular response (AR) and mosaicked BS were derived. Results point at the high...
Tipo: Text Palavras-chave: Multibeam echosounder; Seafloor; Backscatter; Monitoring; Short-term variability; Sensitivity; High-frequency.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59637/62660.pdf
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Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG + 874 A&gt;T polymorphism BJID
Carneiro,Valdirene Leão; Bendicho,Maria Teresita; Santos,Rosalina Guedes; Casela,Marilda; Netto,Eduardo M.; Mota,Scarlet Torres Moraes; Pina,Iza Cristina Araújo; Nascimento,Roberto Meyer; Freire,Songeli Menezes; Barbosa,Theolis.
ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis infection; Genetic variation; Sensitivity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300202
Registros recuperados: 37
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