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| Registros recuperados: 31 | |
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| NODA, R. W.; GUIMARAES, C. T.; GOMES, E. A.; CARNEIRO, N. P.; LANA, U. G. de P.; MAGALHAES, J. V. de; COSTA, M. M. do C.; SILVA, F. R. D.; BRAMMER, S. P.; LÂNGARO, N. C.; CARVALHO, L. J. C. B.; BEVITORI, R.; PURCINO, A. A. C.. |
| bitstream/item/25479/1/Aluminum-induced.pdf |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Tolerância a alumínio; Sequence analysis; Genetics; Aluminum; Genes. |
| Ano: 2010 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/873120 |
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| TISKI, I.; MARRACCINI, P.; POT, D.; VIEIRA, L. G.; PEREIRA, L. F. P.. |
| In higher plants there are two independent pathways for isoprenoid biosynthesis, located in the cytosol (mevalonic acid or MVA pathway) or in the plastids (methylerythritol phosphate ? MEP pathway). The 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) is the first committed step in the MVA pathway. Using the information available from the Brazilian Coffee Genome Project, we found 13 ESTs that originated two isoforms, CaHMGR1 and CaHMGR2, for the enzyme HMGR of Coffea arabica. A complementary DNA encoding the isoform CaHMGR1 was cloned, and its complete nucleotide sequence determined. The full-length cDNA of CaHMGR1 was 2242 bp containing a 1812-bp ORF encoding 604 amino acids. Bioinformatic analyses revealed that the deduced CaHMGR1 had extensive homology... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Gene expression; Sequence analysis; Expressed sequence tag (EST); Molecular characterization; Coffee; Ydroxy; Methyglutaryl-CoA reductase; Isoprenoids. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/902481 |
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| Liu,YongHong; Yang,Bo; Li,XianQiang; Wang,XueFeng; Zhang,LuYao; Li,Fei; Zhao,Li. |
| ABSTRACT In China, Peste des petits ruminants (PPR) was officially first reported in 2007. From 2010 until the outbreak of 2013, PPRV infection was not reported. In November 2013, PPRV re-emerged in Xinjiang and rapidly spread to 22 P/A/M (provinces, autonomous regions and municipalities) of China. In the study, suspected PPRV-infected sheep in a breeding farm of South Xinjiang in 2014 were diagnosed and the characteristics of complete sequence of N protein gene of PPRV was analyzed. The sheep showed PPRV-infected signs, such as fever, orinasal secretions increase, dyspnea and diarrhea, with 60% of morbidity and 21.1% of fatality rate. The macroscopic lesions after autopsy and histopathological changes were observed under light microscope including... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PPR; Diagnosis; N gene; Sequence analysis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501105 |
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| LOBO, F. P.; CINTRA, L. C.; VARELA, E. S.; ALVES, A. L.; VILLELA, L. C. V.; SILVA, N. M. A. da; PAIVA, S. R.; CAETANO, A. R.. |
| The Tambaqui (Colossoma macropomum) is a freshwater fish species naturally found in the Amazon river basin which has historically been widely exploited by community and commercial fishing. Recent efforts to domesticate, breed and raise the species in aquaculture systems has led to significant increases in production (>10-fold) over the last ten years. Current production is above 120,000 metric tons per year with a strong growth trend, making it the most important native aquaculture species in Brazil. Data for generating the draft assembly were produced from shotgun libraries with two different insert sizes and mate-paired libraries with four different sizes sequenced (2x150bps) with Illumina HiSeq2000 technology. A total of 124.8Gbp quality-filtered... |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Sequência genômica; Tambaqui; Genome assembly; Sequence analysis; Colossoma macropomum. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1013201 |
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| CRUZ, I. R. da C.; GERALDO, J. A.; LEITE, L. R.; ZERLOTINI, A.; ARAÚJO, F.; SILVA, M. V. G. B.; COIMBRA, R. S.; CARVALHO, M. R. S.; OLIVEIRA, G.. |
| Recently, we produced the first draft assemblies of Guzerá and Gir genomes. Two Zebu (Bos indicus) breeds adapted to tropical climate and under genetic evaluation to milk purposes in Brazil. |
| Tipo: Resumo em anais de congresso (ALICE) |
Palavras-chave: Sequenciamento de genoma de zebu; Plataforma SOLiD; Genome; Zebu; Sequence analysis. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/946528 |
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| Pérez Ruíz, Elizabeth. |
| El Factor 9 de Crecimiento y Diferenciación (GDF9) es miembro de la súperfamilia β de factores de crecimiento (TGFβ) y su expresión en el ovocito es esencial para el desarrollo y crecimiento folicular. Diferentes mutaciones en el gen GDF9 han sido asociadas con el incremento de tasa de ovulación y/o prolificidad en algunas razas de ovejas, por lo que el objetivo de este estudio fue la búsqueda de polimorfismos de una sola base (SNPs) en el gen GDF9, así como la asociación entre polimorfismos del gen GDF9 y el índice de prolificidad en ovejas de la raza Pelibuey. Se tomaron muestras sanguíneas de la vena yugular de 16 ovejas y fueron conservadas en papel FTA® (Whatman Mini Card). El exón dos del gen fue amplificado mediante la técnica de Polimerase Chain... |
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Palavras-chave: Análisis de secuencias; GDF9; Mutación; Polimorfismo; PCR; SNP; Sequence analysis; Mutation; Polymorphism; Maestría; Ganadería. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1712 |
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| Streck,André Felipe; Souza,Carine Kunzler de; Gonçalves,Karla Rathje; Zang,Luciana; Pinto,Luciane Dubina; Canal,Cláudio Wageck. |
| The presence of canine parvovirus type 2 (CPV-2), 2a and 2b has been described in Brazil, however, the type 2c had not been reported until now. In the current study, seven out of nine samples from dogs with diarrhea were characterized as CPV-2c, indicating that this virus is already circulating in the Brazilian canine population. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine parvovirus type 2c; Sequence analysis; Brazil. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300008 |
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| VOORT, M. van der; MEIJER, H. J. G.; SCHMIDT, Y.; WATROUS, J; DEKKERS, E.; MENDES, R.; DORRESTEIN, P.C; GROSS, H.; RAAIJMAKERS, J. M.. |
| Abstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Pseudomonads; Genomes sequencing; Biocontrol; Rizosfera; Bactéria; Pseudomonas sp; Peptídeo; Beterraba; Controle biológico; Beneficial microorganisms; Rhizosphere bacteria; Antimicrobial peptides; Sequence analysis; Mass spectrometry; Biological control. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1032807 |
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| SILVA, J. M. da; GIACHETTO, P. F.; SILVA, L. O. C. da; CINTRA, L. C.; PAIVA, S. R.; CAETANO, A. R.; YAMAGISHI, M. E. B.. |
| High density genotyping panels have been used in a wide range of applications. From population genetics to genome-wide association studies, this technology still offers the lowest cost and the most consistent solution for generating SNP data. However, in spite of the application, part of the generated data is always discarded from final datasets based on quality control criteria used to remove unreliable markers. Some discarded data consists of markers that failed to generate genotypes, labeled as missing genotypes. A subset of missing genotypes that occur in the whole population under study may be caused by technical issues but can also be explained by the presence of genomic variations that are in the vicinity of the assayed SNP and that prevent... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Polimorfismo de nucleotídeo único; Sequenciamento de DNA; Gado de corte; Single nucleotide polymorphism; Bos indicus; Sequence analysis; Cattle. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1033982 |
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| Wang,Xiran; Pei,Yu; Dou,Jingtao; Lu,Juming; Li,Jian; Lv,Zhaohui. |
| Osteogenesis imperfecta (OI) is a family of genetic disorders associated with bone loss and fragility. Mutations associated with OI have been found in genes encoding the type I collagen chains. People with OI type I often produce insufficient α1-chain type I collagen because of frameshift, nonsense, or splice site mutations in COL1A1 or COL1A2. This report is of a Chinese daughter and mother who had both experienced two bone fractures. Because skeletal fragility is predominantly inherited, we focused on identifying mutations in COL1A1 and COL1A2 genes. A novel mutation in COL1A1, c.700delG, was detected by genomic DNA sequencing in the mother and daughter, but not in their relatives. The identification of this mutation led to the conclusion that they were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Osteogenesis imperfecta; Chinese OI type 1 family; Type I collagen; Sequence analysis; Frameshift mutation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100001 |
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| Jie,Li; Zheng,Ping-Ping; Song,Jiao-Lian; Rui,Jin-Long; Nie,Liu-Wang. |
| The Sox family of genes shares a high sequence similarity with the HMG box region of the human Y chromosomal gene, SRY. We used highly degenerate primers to clone and sequence seven Eremias breuchleyi Sox genes (EbSox2, EbSox3, EbSox4, EbSox11, EbSox12, EbSox14 and EbSox21). A database search for the cloned sequences revealed the following percentage identity with the homologous human SOX genes: EbSox2 = 96%, EbSox3 = 88%, EbSox4 = 94%, EbSox11 = 99%, EbSox12 = 96%, EbSox14 = 98%, EbSox21 = 97%. Cluster analysis indicates that they seem to belong to group B and group C of Sox gene family, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eremias breuchleyi; PCR; Sequence analysis; Sox genes; SSCP. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300031 |
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| VIEIRA, A. F.; PANTALIÃO, G. F.; ABREU, F. M.; SILVEIRA, R. D.; GARCIA, A. L. B.; NEVES, L. G.; VIANELLO, R. P.; CASTRO, A. P. de; BRONDANI, C.. |
| Breeding for water-deficit tolerance is fundamental to guarantee the sustainability of upland rice production, mainly due to the possibility of an increased frequency of drought episodes due to climate change. This work aimed to identify single nucleotide polymorphism (SNP) markers, derived from RNA sequencing (RNA-Seq), genome-wide association study (GWAS) and candidate genes from Arabidopsis, with potential for use in marker-assisted selection (MAS) for drought tolerance. RNA-Seq and GWAS were efficient in identifying useful SNP markers from the data obtained from three years of field experiments for 175 upland rice accessions, which were sequenced using 32 genes by Capture-Seq. Three genes were equally able to generate SNP markers that discriminated 95%... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Arroz; Melhoramento genético vegetal; Resistência a seca; Rice; Genotyping by sequencing; Plant breeding; Genetic techniques and protocols; Single nucleotide polymorphism; Nucleotide sequences; Sequence analysis; Drought tolerance. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088846 |
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| Henkes,Luiz Ernani; Silva Jr,Wilson Araújo; Moraes,José Carlos Ferrugem; Weimer,Tania Azevedo. |
| The genetic diversity of 277 nucleotides in the mitochondrial DNA control region (nt 15,964 to 16,240 in reference sequence) was analyzed in crossbreed beef cattle (Brangus-Ibage, 5/8 Bos primigenius taurus x 3/8 Bos primigenius indicus) as well as in some Nellore samples (B. p. indicus). Fifty-seven mutations were found in Brangus-Ibage comprising 18 haplotypes (haplotype diversity, h = 0.851 ± 0.041 and nucleotide diversity, ntd = 0.009 ± 0.006) and 66 in Nellore (h = 1.00 ± 0.27, ntd = 0.014 ± 0.012). These data indicated sequence identities of 99.6 and 92.1% between the B. p. taurus' reference sequence and Brangus-Ibage and Nellore, respectively. The comparison of our data with sequence data for 612 individuals recovered from GenBank showed a total of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine mtDNA; Maternal lineage; Sequence analysis; Beef cattle; Genetic diversity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100011 |
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| Réblová, M.; Réblová, K.; Štěpánek, V.. |
| Thirteen morphologically similar strains of barbatosphaeria- and tectonidula-like fungi were studied based on the comparison of cultural and morphological features of sexual and asexual morphs and phylogenetic analyses of five nuclear loci, i.e. internal transcribed spacer rDNA operon (ITS), large and small subunit nuclear ribosomal DNA, β-tubulin, and second largest subunit of RNA polymerase II. Phylogenetic results were supported by in-depth comparative analyses of common core secondary structure of ITS1 and ITS2 in all strains and the identification of non-conserved, co-evolving nucleotides that maintain base pairing in the RNA transcript. Barbatosphaeria is defined as a well-supported monophyletic clade comprising several lineages and is placed in the... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Phylogenetics; Ramichloridium; Sequence analysis; Spacer regions; Sporothrix; Tectonidula. |
| Ano: 2015 |
URL: http://www.repository.naturalis.nl/record/588698 |
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| Registros recuperados: 31 | |
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