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| Registros recuperados: 35 | |
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| Viancelli,Aline; Kunz,Airton; Esteves,Paulo Augusto; Bauermann,Fernando Viçosa; Furukawa,Kenji; Fujii,Takao; Antônio,Regina Vasconcellos; Vanotti,Matias. |
| The present study aimed to describe the bacterial community present at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated with the sludge from the anaerobic pond of a swine slurry treatment system. The description was based on the molecular DNA techniques using primers for amplification of complete 16S rRNA gene and also new primers to amplify smaller fragments from 16S rRNA. During the bioreactor operation time, the bacterial community changed significantly, increasing the nitrogen removal efficiency, reaching after 500 days a removal rate of 94%. The complete PCR amplification of 16S rRNA gene generated 17 clones, where three presented similarity with Candidatus Jettenia asiatica (97%), twelve with Janthinobacterium (99%) and two with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ANAMMOX; Anaerobic pond; Sequencing; Swine manure. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500022 |
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| Ruiz,Ana Zuley. |
| The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cloning; NMDA receptor; Ovis aries; Ram; Sequencing. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183 |
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| Villarreal,LYB; Brandão,PEB; Chacón,JLV; Doretto Junior,L; Ito,N; Gama,NS; Ishizuka,MM; Luchese,A; Buchala,F; Astolfi-Ferreira,CS; Ferreira,AJP. |
| Avian Infectious Laryngotracheitis, caused by Infectious Laryngotracheitis Virus (ILTV), has been reported for decades in Brazilian laying and broiler flocks. More recently, outbreaks have occurred in São Paulo State. This study reports the application of PCR and DNA sequencing targeted to the p32 gene of ILTV using laying chicken samples from Bastos, São Paulo, Brazil. Three out of four field samples were positive by PCR. DNA sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of ILTV. The results definitely confirmed the presence of ILTV in the birds during the outbreak. Further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian infectious laryngotracheitis; Brazil; P32; PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400011 |
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| SANTOS,LIDIANE F. DOS; LANA,ROGÉRIO P.; SILVA,MARLIANE C.S. DA; VELOSO,TOMÁS G.R.; KASUYA,MARIA CATARINA M.; RIBEIRO,KARINA G.. |
| Abstract Effective microorganisms (EM) are inoculants formed by fungi and bacteria isolated from soil. EM are commonly used by farmers on agronomic crops to stimulate plant growth, but their composition and their benefits has been controverted. This study aimed to analyze the diversity of microorganisms growing in three EM inoculants, as well as to evaluate their efficiency in the germination of palisade grass seeds. The total DNA of the three EM inoculants was extracted, the 16S rRNA and ITS genes were amplified by PCR and sequenced on the Illumina MiSeq platform. Germination tests were conducted with three type of the EM, in three concentration and two times of the immersion. The bacterial group was the most abundant in EM, followed by fungi. Bacterial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diversity; Dormancy; Sequencing; Sulfuric acid. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000201002 |
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| Hernández Deheza, María Guadalupe. |
| En Yurécuaro, Michoacán, en el ciclo de cultivo verano otoño 2009 y 2011 se encontraron en cultivos de chile síntomas de variegado, nervaduras cloróticas, deformación de la lámina foliar y fruto, albinismo y escaldadura, síndrome al cual se le denominó “variegado del chile”. A simple vista, esta serie de síntomas indicaban que el problema era causado por virus fitopatógenos; sin embargo, en ensayos previos se detectó a Candidatus Liberibacter solanacearum, por lo que el objetivo del presente trabajo fue determinar si esta bacteria es el agente causal del variegado del chile. Mediante transmisión por injerto, transmisión con el vector Bactericera cockerelli y reacción en cadena de la polimerasa con el par de iniciadores OA2/CLi.po.F y OI2, se confirmo que... |
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Palavras-chave: Candidatus Liberibacter; PCR; Secuenciación; Sequencing; Entomología y Acarología; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1844 |
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| Chibeu,Andrew; Lavigne,Rob; Mattheus,Wesley; Matthijs,Sandra; Cornelis,Pierre; Volckaert,Guido. |
| We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries. |
| Tipo: Journal article |
Palavras-chave: Cosmid; PRG930cm; Pseudomonas; Sequencing. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200010 |
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| Deef, Lamiaa Elsayed Mokhtar. |
| This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra, Gallinulla angulata, Gallinulla chloropus and Porphyrio porphyrio. Mitochondrial cytochrome oxidase subunit I (COI) sequences were used as an effective marker in this study. DNA of Rallidae species were extracted, amplified using polymerase chain reaction (PCR) and then sequenced. The results obtained from information based on COI sequences revealed that Gallinulla angulata and Gallinulla chloropus fall into two separate clades and they are not monophyletic. This suggests that, two moorhens could not be laid into the same genus. In addition, Porphyrio porphyrio was included in the same genus with Porphyrio alleni but they were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Gruiformes; PCR; Phylogeny; Sequencing; Species.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37184 |
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| Miagostovich,M.P.; Santos,F.B. dos; Simone,T.S. de; Costa,E.V.; Filippis,A.M.B.; Schatzmayr,H.G.; Nogueira,R.M.R.. |
| The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Dengue viruses type 3; Sequencing; RSS-PCR; Rio de Janeiro; Brazil. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800002 |
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| Chudobova,Dagmar; Cihalova,Kristyna; Guran,Roman; Dostalova,Simona; Smerkova,Kristyna; Vesely,Radek; Gumulec,Jaromir; Masarik,Michal; Heger,Zbynek; Adam,Vojtech; Kizek,Rene. |
| ABSTRACT BACKGROUND: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n = 50) presenting with bacterial infection. METHODS: After successful cultivation, bacteria were processed and analyzed. Initially the identification of the strains was performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on comparison of protein profiles (2-30 kDa) with database. Subsequently, bacterial strains from infected wounds were identified by both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial strains; MALDI-TOF; Sequencing; Superficial wounds. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600604 |
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| Coêlho,Mariza M.; Ferreira-Nozawa,Monica S.; Nozawa,Sérgio R.; Santos,André L.W.. |
| Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Sequencing; Bacillus; Pantoea. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400021 |
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| Gombac, Mitja; Kusar, D.; Ocepek, M.; Pogacnik, Milan; Arzul, Isabelle; Couraleau, Yann; Jenclc, V.. |
| Among 1280 cultured and wild adult Mediterranean mussels, Mytilus galloprovincialis, collected over a 1-year surveillance period from the Slovene Adriatic Sea, 0.3% were histologically positive for the presence of Marteilia spp. The infection was concentrated in winter. Employing the molecular methods of PCR, cloning, DNA restriction and sequencing, only Marteilia refringens type M was detected in all the infected mussels. Although all life-cycle stages of M. refringens severely infected digestive glands, only sporadic disruption of epithelial cells of digestive tubules and focal destruction of digestive tubules were observed in the infected mussels. This was the first detection of M. refringens in M. galloprovincialis from the Slovene Adriatic Sea with... |
| Tipo: Text |
Palavras-chave: Prevalence; Marteilia refringens type M.; Mediterranean mussels Mytilus galloprovincialis; Sequencing; Severe infection; Slovene Adriatic Sea. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00165/27640/26064.pdf |
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| Registros recuperados: 35 | |
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