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Registros recuperados: 13
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Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa OAK
NGUYEN, Thu-Thuy; MOTSIRI, Mono Sophie; TAIOE, Moeti Oriel; MTSHALI, Moses Sibusiso; GOTO, Yasuyuki; KAWAZU, Shin-Ichiro; Molifi THEKISOE, Oriel Matlhahane; INOUE, Noboru; 河津, 信一郎; 井上, 昇.
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant...
Palavras-chave: Animal African trypanosomosis; ELISA; Immunochromatographic test; Serodiagnosis; South Africa.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4034
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Canine visceral leishmaniosis in an area of fishing tourism, Bonito Municipality, Mato Grosso do Sul, Central-West Brazil Ciência Rural
Brilhante,Andreia Fernandes; Dorval,Maria Elizabeth Cavalheiros; Galati,Eunice Aparecida Bianchi; Castro,Vivianne de Oliveira Landgraf de; Rocca,Maria Elizabeth Ghizzi; Facco,Gilberto Gonçalves; Souza,Celeste da Silva Freitas de; Nunes,Vânia Lúcia Brandão.
ABSTRACT: The study aimed to investigate Leishmania infection in a population of dogs (92 animals) in a fishing area of Bonito Municipality, and evaluate comparatively the serological methods used, immunoenzymatic assay (ELISA), indirect immunofluorescence antibody test (IFAT), and rapid Dual Path-Platform test (DPP®). Blood and aspirate of bone marrow samples were used and parasitological investigation was also performed, such as parasite isolation in Neal, Novy, Nicolle (NNN) medium culture, Woo technique, Giemsa stained smears and specific identification by polymerase chain reaction (PCR). IFAT revealed 56/92 seropositive, the ELISA 8/92 and the DPP®41/92. Regarding the agreement between the serological tests by the Kappa index, there was a slight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Domestic dogs; Serodiagnosis; Ecotourism.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300501
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Conventional serological performance in diagnosis of Chagas' disease in southern Brazil BJID
Araújo,Anelise Bergmann; Berne,Maria Elisabeth Aires.
Results of Chagas' disease diagnosis show disagreement. The aim of this study was to compare commercial tests for Chagas' disease serodiagnosis in southern Brazil. A total of 161 samples were evaluated. Three enzyme-linked immunosorbent assays, one indirect hemagglutination and one indirect immunofluorescence were assessed. Trypomastigote excreted-secreted antigen-blot was a confirmatory method. From 161 samples, 65.84% were positive in all tests, while 34.16% presents mismatch result in at least one of the tests. All techniques tested presented false-positive and/or false-negative results as follows: Enzyme-linked immunosorbent assay 1 had more false-positive results (lower specificity), indirect immunofluorescence had the highest rate of false-negative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Chagas' disease; Serodiagnosis; Immunoassays.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200010
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Epidemiologic and clinical characteristics of pregnant women living with HIV/AIDS in a region of Southern Brazil where the subtype C of HIV-1 infection predominates BJID
Manenti,Sandra Aparecida; Galato Júnior,João; Silveira,Elizângela da Silva; Oenning,Roberto Teixeira; Simões,Priscyla Waleska Targino de Azevedo; Moreira,Jeverson; Fochesato,Celine Maria; Brígido,Luís Fernando de Macedo; Rodrigues,Rosângela; Romão,Pedro Roosevelt Torres.
Southern Brazil has the highest prevalence rate of AIDS in the country and is the only region in the Americas where HIV-1 subtype C prevails. OBJECTIVE: We evaluated the epidemiologic and clinical characteristics of pregnant women living with HIV/AIDS in the South region of Santa Catarina, Brazil. METHODS: All pregnant women with HIV infection attending the obstetric outpatient clinic of Criciúma, State of Santa Catarina, in 2007 (n = 46) were invited to participate. Data of 36 eligible participants were obtained through a standardized questionnaire. RESULTS: The great majority were young, with a steady partner, low family income, low education level and referring early first sexual intercourse. Many reported use of illicit non-injecting drugs (55.5%) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS; Serodiagnosis; HIV infections; Pregnancy; HIV-1 subtype C; Southern Brazil.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400008
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Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis OAK
Abdelbaset, Abdelbaset Eweda; Alhasan, Hend; Salman, Doaa; Karram, Mohamed Hassan; Rushdi, Mahmoud Abd Ellah; Xuan Xuenan; Igarashi, Makoto.
Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in...
Palavras-chave: ELISA; Recombinant proteins; Serodiagnosis; Toxoplasmosis.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4574
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Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis) Ciência Rural
Paulin,Lília Márcia Silva; Samartino,Luis Ernesto; Conde,Sandra Beatriz; Federsoni,Igor Stefan Poppovic; Ferreira,Fernando; Amaku,Marcos; Ferreira Neto,José Soares.
The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also calculated. The best combinations of relative sensitivity (SEr) and relative specificity (SPr) and Kappa were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serological tests; Serodiagnosis; Buffaloes; Brucellosis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900017
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Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis OAK
Jia, Honglin; Zhou, Jinlin; Ikadai, Hiromi; Matsuu, Aya; Suzuki, Hiroshi; Fujisaki, Kozo; Xuan, Xuenan; 鈴木, 宏志; 五十嵐, 郁男; 玄, 学南.
Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These...
Palavras-chave: Babesia gibsoni; Secreted Antigen 1; Identification; Serodiagnosis.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1655
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Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis BJMBR
Celeste,B.J.; Angel,S.O.; Castro,L.G.M.; Gidlund,M.; Goto,H..
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Leishmania infantum; Sp 83; ELISA; Serodiagnosis; Tegumentary leishmaniasis; Chagas' disease.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100001
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Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent BJM
Hoshino-Shimizu,Sumie; Vaz-de-Lima,Lourdes Rehder de Andrade; Oliveira,Maria Isabel de; Pereira,Carlos Augusto; Moura,Anísio de; Mendonça,Ronaldo Zucatelli.
Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL, using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Measles; IgM-measles reagent; Reagent quality control; Serodiagnosis; Seroepidemiology.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016
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Preliminary studies for development of an immunochromatographic test (ICT) for detection of antibody against salivarian trypanosomes by using recombinant ribosomal P0 protein OAK
Cheng, Z; Goto, Y; Nguyen, T; Sakurai, T; Zang, J; Kawazu, S; Inoue, N; 河津, 信一郎; 井上, 昇.
http://www.obihiro.ac.jp/~protozoa/index.html
Palavras-chave: Trypanosomosis; Serodiagnosis; Immunechromatography.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3540
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Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis? BJMBR
Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A..
Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705
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Serodiagnosis of Helicobacter pylori infection by Cobas Core ELISA in adults from Minas Gerais, Brazil BJMBR
Rocha,G.A.; Oliveira,A.M.R.; Queiroz,D.M.M.; Mendes,E.N.; Moura,S.B.; Oliveira,C.A.; Ferrari,T.C.A..
We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from a developing country in view of variations in sensitivity and specificity reported for different populations. We studied 97 non-consecutive patients who underwent endoscopy for evaluation of dispeptic symptoms. The presence of H. pylori was determined in antral biopsy specimens by culture, by the preformed urease test and in carbolfuchsin-stained smears. Patients were considered to be H. pylori positive if at least two of the three tests presented a positive result or if the culture was positive, and negative if the three tests were negative. Sixty-five adults (31 with peptic ulcer) were H. pylori positive and 32 adults were H. pylori negative....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter pylori; Serodiagnosis; ELISA.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000005
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The importance of serological assays in diagnosing acute pulmonary histoplasmosis J. Venom. Anim. Toxins incl. Trop. Dis.
Freitas,RS; Carvalho-Vivi,JO; Zamboni,IM; Assis,CM; Costa-Martins,JE; Vicentini-Moreira,AP.
Histoplasmosis is a systemic mycosis caused by inhalation of Histoplasma capsulatum microconidia. The disease does not normally affect immunocompetent individuals after a single, transient inhalation exposure. However, longer exposure may cause chronic or disseminated acute pulmonary infection. Herein, we report the case of a 24-year-old immunocompetent patient, who presented fever, cough and dyspnea for one month. The chest radiography revealed interstitial infiltrate and diffuse micronodules. The patient reported having had close and prolonged contact with bats. Diagnosis was confirmed by positive double immunodifusion and immunoblotting assays. She was treated with ketoconazole (400 mg) and there was complete resolution of the disease.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histoplasmosis; Histoplasma capsulatum; Immunocompetence; Serodiagnosis; Bats.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200010
Registros recuperados: 13
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